Reviewed, UniProtKB/Swiss-Prot P33282 (URIC_EMENI)

Names and origin

Protein names

Recommended name:
    Uricase
    EC=1.7.3.3
Alternative name(s):
    Urate oxidase

Gene names

Name:	uaZ
ORF Names:	AN9470

Organism

Emericella nidulans (Aspergillus nidulans)

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which spontaneously decomposes to form allantoin.
Catalytic activity	Urate + O2 + H2O = 5-hydroxyisourate + H2O2.
Pathway	Purine metabolism; uric acid degradation; (S)-allantoin from uric acid: step 1/3.
Subcellular location	Peroxisome.
Sequence similarities	Belongs to the uricase family.

Ontologies

Keywords
Biological process	Purine metabolism
Cellular component	Peroxisome
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	urate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Uricase		PRO_0000165996
Regions
	Region	228 – 229	2	Substrate binding By similarity
	Motif	299 – 301	3	Microbody targeting signal Potential
Sites
	Active site	177	1	Charge relay system By similarity
	Active site	229	1	Charge relay system By similarity
	Binding site	58	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33282-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: BA46DD84925BCD9F	FASTA	301	34,003
	10 20 30 40 50 60 MSTVAAARYG KDNVRVYKVH KDPKTGVQTV TEMTVCVLLE GEIDTSYTKA DNSVIVATDS 70 80 90 100 110 120 IKNTIFILAK QNPVTPPELF GSILGTHFIN KYKHIHVAHT NIITHRWTRL NIDGKPHSHS 130 140 150 160 170 180 FVRDSEETRN VQVDVTEGVG IDIKSSINKL TVLKSTGSQF WGFVRDEYTT LPEVWDRILS 190 200 210 220 230 240 TDVEATWAWK RFSGLDEVRG NVPKFDETWE AARNITLKTF AEEESASVQA TMYKMGEQIL 250 260 270 280 290 300 AYQPLLETVE YSLPNKHYFE IDLSWHKGLK NTGKDAEVFV PQTNPNGLIK CTVGRKSKAK L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, regulation, and mutational analysis of the gene encoding urate oxidase in Aspergillus nidulans." Oestreicher N., Scazzocchio C. J. Biol. Chem. 268:23382-23389(1993) [PubMed: 8226863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X72210 Genomic DNA. Translation: CAA51009.1. AACD01000194 Genomic DNA. Translation: EAA57588.1.
PIR	A48879.
3D structure databases
HSSP	HSSP built from PDB template 1UOX based on UniProtKB Q00511.
SMR	P33282. Positions 2-295.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.3.3. 3859.
Family and domain databases
InterPro	IPR002042. Uricase. IPR019842. Uricase_CS. [Graphical view]
Gene3D	G3DSA:3.10.270.10. Uricase. 1 hit.
PANTHER	PTHR10395:SF1. Uricase. 1 hit.
Pfam	PF01014. Uricase. 2 hits. [Graphical view]
PRINTS	PR00093. URICASE.
ProDom	PD003367. Uricase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03383. urate_oxi. 1 hit.
PROSITE	PS00366. URICASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URIC_EMENI

Accession

Primary (citable) accession number: P33282
Secondary accession number(s): Q5AQG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)