Reviewed, UniProtKB/Swiss-Prot P33280 (ARGI_NEUCR)

Names and origin

Protein names

Recommended name:
    Arginase
    EC=3.5.3.1

Gene names

Name:	car-1
Synonyms:	aga
ORF Names:	NCU02333

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Subunit structure	Homohexamer.
Subcellular location	Cytoplasm. Ref.1
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Cellular component	Cytoplasm
Coding sequence diversity	Alternative initiation
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arginase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 41 kDa (identifier: P33280-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 36 kDa (identifier: P33280-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Arginase		PRO_0000002087
Regions
	Region	176 – 180	5	Substrate binding By similarity
	Region	187 – 189	3	Substrate binding By similarity
Sites
	Metal binding	146	1	Manganese 1 By similarity
	Metal binding	174	1	Manganese 1 By similarity
	Metal binding	174	1	Manganese 2 By similarity
	Metal binding	176	1	Manganese 2 By similarity
	Metal binding	178	1	Manganese 1 By similarity
	Metal binding	280	1	Manganese 1 By similarity
	Metal binding	280	1	Manganese 2 By similarity
	Metal binding	282	1	Manganese 2 By similarity
	Binding site	233	1	Substrate By similarity
	Binding site	325	1	Substrate By similarity
Natural variations
	Alternative sequence	1 – 33	33	Missing in isoform 36 kDa.		VSP_018650
Experimental info
	Sequence conflict	351 – 358	8	CALGESLL → SRSRRNVL in AAC82503. Ref.1
	Sequence conflict	351 – 358	8	CALGESLL → SRSRRNVL in AAC82504. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 41 kDa [UniParc]. Last modified August 31, 2004. Version 3. Checksum: 259CF279863D6977	FASTA	358	38,265
	10 20 30 40 50 60 MSPSLVDNHA AAYIAAPSSA KAPMIQKPGN TFGMSSPIES KFLSQPRDLG IVAVGFSGGQ 70 80 90 100 110 120 CKPGVDAAPS ALIESGLLTQ LREELGYRLH GDDEVHLYTD LVPKEDPPHR NMKNPRAVSN 130 140 150 160 170 180 VTKRIAEQVH SHAKEGRLVL TLGGDHSIAI GTIAGSAKAI KERLGREIAV IWVDAHADIN 190 200 210 220 230 240 TPETSGSGNI HGMPVSFLTG LASEDKEEFF GWLKPDHLLS VKKLVYIGLR DVDPGEKRIL 250 260 270 280 290 300 RENGIKAFSM HDIDKHGIGR VMEMALGHIG NDTPIHLSFD VDALDPMWAP STGTPVRGGL 310 320 330 340 350 TLREGDFICE CVHETGSLVA VDLVEVNPTL AAPNDVGAHE TVRAGCSLVR CALGESLL
	Isoform 36 kDa. Checksum: E6FFB8F34AAEDC8C	FASTA	325	34,939
	10 20 30 40 50 60 MSSPIESKFL SQPRDLGIVA VGFSGGQCKP GVDAAPSALI ESGLLTQLRE ELGYRLHGDD 70 80 90 100 110 120 EVHLYTDLVP KEDPPHRNMK NPRAVSNVTK RIAEQVHSHA KEGRLVLTLG GDHSIAIGTI 130 140 150 160 170 180 AGSAKAIKER LGREIAVIWV DAHADINTPE TSGSGNIHGM PVSFLTGLAS EDKEEFFGWL 190 200 210 220 230 240 KPDHLLSVKK LVYIGLRDVD PGEKRILREN GIKAFSMHDI DKHGIGRVME MALGHIGNDT 250 260 270 280 290 300 PIHLSFDVDA LDPMWAPSTG TPVRGGLTLR EGDFICECVH ETGSLVAVDL VEVNPTLAAP 310 320 NDVGAHETVR AGCSLVRCAL GESLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple forms of arginase are differentially expressed from a single locus in Neurospora crassa." Marathe S.V., Yu Y.G., Turner G.E., Palmier C., Weiss R.L. J. Biol. Chem. 273:29776-29785(1998) [PubMed: 9792692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE INITIATION, SUBCELLULAR LOCATION. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"Purification and characterization of arginase from Neurospora crassa." Borkovich K.A., Weiss R.L. J. Biol. Chem. 262:7081-7086(1987) [PubMed: 2953715] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L20687 Genomic DNA. Translation: AAC82503.1. L20687 Genomic DNA. Translation: AAC82504.1. AABX02000010 Genomic DNA. Translation: EAA30523.2. Different initiation.
PIR	T47228. T47229.
3D structure databases
HSSP	HSSP built from PDB template 1D3V based on UniProtKB P07824.
ModBase	Search...
Protein-protein interaction databases
STRING	P33280.
Genome annotation databases
NMPDR	fig|5141.1.peg.9042.
UCSC	Y18D10A.17. c. elegans.
Enzyme and pathway databases
BRENDA	3.5.3.1. 266.
Family and domain databases
InterPro	IPR005924. Arginase. IPR014033. Arginase_sub. IPR006035. Ureohydrolase. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. rocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGI_NEUCR

Accession

Primary (citable) accession number: P33280
Secondary accession number(s): Q7RV08, Q9URL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	August 31, 2004
Last modified:	September 1, 2009
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)