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P33274 (CP4F1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytochrome P450 4F1
EC=1.14.14.1
Alternative name(s):
CYPIVF1
Cytochrome P450-A3
Gene names
Name:Cyp4f1
Synonyms:Cyp4f-1, Cyp4f2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Developmental stage

The level of P450 4F1 is found to increase dramatically in hepatic tumors.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 524524Cytochrome P450 4F1
PRO_0000051849

Sites

Metal binding4681Iron (heme axial ligand)
Binding site3281Heme (covalent; via 1 link)

Sequences

Sequence LengthMass (Da)Tools
P33274 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 572CEA2617CE632D

FASTA52459,868
        10         20         30         40         50         60 
MSQLSLSWLG LGPEVAFPWQ TLLLFGASWI LAQILTQIYA AYRNFRRLRG FPQPPKRNWL 

        70         80         90        100        110        120 
MGHVGMVTPT EQGLKELTRL VGTYPQGFLM WIGPMVPVIT LCHSDIVRSI LNASAAVALK 

       130        140        150        160        170        180 
DVIFYTILKP WLGDGLLVSA GDKWSRHRRM LTPAFHFNIL KPYVKIFNDS TNIMHAKWKR 

       190        200        210        220        230        240 
LISEGSSRLD MFEHVSLMTL DSLQKCVFSF DSNCQEKSSE YIAAILELSA LVAKRHQQPL 

       250        260        270        280        290        300 
LFMDLLYNLT PDGMRFHKAC NLVHEFTDAV IRERRRTLPD QGLDEFLKSK AKSKTLDFID 

       310        320        330        340        350        360 
VLLLTKDEDG KELSDEDIRA EADTFMFEGH DTTASGLSWI LYNLANDPEY QERCRQEVQE 

       370        380        390        400        410        420 
LLRDRDPEEI EWDDLAQLPF LTMCIKESLR LHPPVTVISR CCTQDILLPD GRTIPKGIIC 

       430        440        450        460        470        480 
LISIFGIHHN PSVWPDPEVY NPFRFDPENI KDSSPLAFIP FSAGPRNCIG QTFAMSEMKV 

       490        500        510        520 
ALALTLLRFR LLPDDKEPRR QPELILRAEG GLWLRVEPLT AGAQ

« Hide

References

[1]"Identification of a new P450 subfamily, CYP4F1, expressed in rat hepatic tumors."
Chen L., Hardwick J.P.
Arch. Biochem. Biophys. 300:18-23(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Hepatoma.
[2]"Structure and transcriptional activity of the CYP4F1 leukotriene omega hydroxylase gene."
Donelson E.L., Wu Y., Vernell R., Chen L., Hardwick J.P.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
[3]"Covalent attachment of the heme prosthetic group in the CYP4F cytochrome P450 family."
LeBrun L.A., Xu F., Kroetz D.L., Ortiz de Montellano P.R.
Biochemistry 41:5931-5937(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: COVALENT HEME ATTACHMENT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M94548 mRNA. Translation: AAA41040.1.
AF200361 Genomic DNA. Translation: AAF20822.1.
IPIIPI00189753.
PIRS29723.
UniGeneRn.5722.

3D structure databases

ProteinModelPortalP33274.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-4569528.

Proteomic databases

PaxDbP33274.
PRIDEP33274.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:70926. rat.

Organism-specific databases

RGD70926. Cyp4f1.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000233833.
HOVERGENHBG000182.
InParanoidP33274.
OrthoDBEOG40CHGX.

Gene expression databases

ArrayExpressP33274.
GenevestigatorP33274.
GermOnlineENSRNOG00000004786. Rattus norvegicus.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. Cytochrome_P450. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP4F1_RAT
AccessionPrimary (citable) accession number: P33274
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 29, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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