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P33274

- CP4F1_RAT

UniProt

P33274 - CP4F1_RAT

Protein

Cytochrome P450 4F1

Gene

Cyp4f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei328 – 3281Heme (covalent; via 1 link)
    Metal bindingi468 – 4681Iron (heme axial ligand)

    GO - Molecular functioni

    1. arachidonic acid binding Source: RGD
    2. arachidonic acid monooxygenase activity Source: RGD
    3. aromatase activity Source: UniProtKB-EC
    4. heme binding Source: RGD
    5. iron ion binding Source: InterPro

    GO - Biological processi

    1. arachidonic acid metabolic process Source: RGD
    2. icosanoid metabolic process Source: RGD
    3. response to toxic substance Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 4F1 (EC:1.14.14.1)
    Alternative name(s):
    CYPIVF1
    Cytochrome P450-A3
    Gene namesi
    Name:Cyp4f1
    Synonyms:Cyp4f-1, Cyp4f2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi70926. Cyp4f1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. intracellular membrane-bounded organelle Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 524524Cytochrome P450 4F1PRO_0000051849Add
    BLAST

    Proteomic databases

    PaxDbiP33274.
    PRIDEiP33274.

    Expressioni

    Developmental stagei

    The level of P450 4F1 is found to increase dramatically in hepatic tumors.

    Gene expression databases

    GenevestigatoriP33274.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4569528.

    Structurei

    3D structure databases

    ProteinModelPortaliP33274.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000233833.
    HOVERGENiHBG000182.
    InParanoidiP33274.
    PhylomeDBiP33274.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33274-1 [UniParc]FASTAAdd to Basket

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    MSQLSLSWLG LGPEVAFPWQ TLLLFGASWI LAQILTQIYA AYRNFRRLRG    50
    FPQPPKRNWL MGHVGMVTPT EQGLKELTRL VGTYPQGFLM WIGPMVPVIT 100
    LCHSDIVRSI LNASAAVALK DVIFYTILKP WLGDGLLVSA GDKWSRHRRM 150
    LTPAFHFNIL KPYVKIFNDS TNIMHAKWKR LISEGSSRLD MFEHVSLMTL 200
    DSLQKCVFSF DSNCQEKSSE YIAAILELSA LVAKRHQQPL LFMDLLYNLT 250
    PDGMRFHKAC NLVHEFTDAV IRERRRTLPD QGLDEFLKSK AKSKTLDFID 300
    VLLLTKDEDG KELSDEDIRA EADTFMFEGH DTTASGLSWI LYNLANDPEY 350
    QERCRQEVQE LLRDRDPEEI EWDDLAQLPF LTMCIKESLR LHPPVTVISR 400
    CCTQDILLPD GRTIPKGIIC LISIFGIHHN PSVWPDPEVY NPFRFDPENI 450
    KDSSPLAFIP FSAGPRNCIG QTFAMSEMKV ALALTLLRFR LLPDDKEPRR 500
    QPELILRAEG GLWLRVEPLT AGAQ 524
    Length:524
    Mass (Da):59,868
    Last modified:February 1, 1994 - v1
    Checksum:i572CEA2617CE632D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94548 mRNA. Translation: AAA41040.1.
    AF200361 Genomic DNA. Translation: AAF20822.1.
    PIRiS29723.
    UniGeneiRn.5722.

    Genome annotation databases

    UCSCiRGD:70926. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94548 mRNA. Translation: AAA41040.1 .
    AF200361 Genomic DNA. Translation: AAF20822.1 .
    PIRi S29723.
    UniGenei Rn.5722.

    3D structure databases

    ProteinModelPortali P33274.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4569528.

    Proteomic databases

    PaxDbi P33274.
    PRIDEi P33274.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:70926. rat.

    Organism-specific databases

    RGDi 70926. Cyp4f1.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000233833.
    HOVERGENi HBG000182.
    InParanoidi P33274.
    PhylomeDBi P33274.

    Gene expression databases

    Genevestigatori P33274.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a new P450 subfamily, CYP4F1, expressed in rat hepatic tumors."
      Chen L., Hardwick J.P.
      Arch. Biochem. Biophys. 300:18-23(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Hepatoma.
    2. "Structure and transcriptional activity of the CYP4F1 leukotriene omega hydroxylase gene."
      Donelson E.L., Wu Y., Vernell R., Chen L., Hardwick J.P.
      Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
    3. "Covalent attachment of the heme prosthetic group in the CYP4F cytochrome P450 family."
      LeBrun L.A., Xu F., Kroetz D.L., Ortiz de Montellano P.R.
      Biochemistry 41:5931-5937(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: COVALENT HEME ATTACHMENT.

    Entry informationi

    Entry nameiCP4F1_RAT
    AccessioniPrimary (citable) accession number: P33274
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3