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P33274

- CP4F1_RAT

UniProt

P33274 - CP4F1_RAT

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Protein

Cytochrome P450 4F1

Gene

Cyp4f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

heme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei328 – 3281Heme (covalent; via 1 link)1 Publication
Metal bindingi468 – 4681Iron (heme axial ligand)1 Publication

GO - Molecular functioni

  1. arachidonic acid binding Source: RGD
  2. arachidonic acid monooxygenase activity Source: RGD
  3. aromatase activity Source: UniProtKB-EC
  4. heme binding Source: RGD
  5. iron ion binding Source: InterPro

GO - Biological processi

  1. arachidonic acid metabolic process Source: RGD
  2. icosanoid metabolic process Source: RGD
  3. response to toxic substance Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4F1 (EC:1.14.14.1)
Alternative name(s):
CYPIVF1
Cytochrome P450-A3
Gene namesi
Name:Cyp4f1
Synonyms:Cyp4f-1, Cyp4f2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi70926. Cyp4f1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. intracellular membrane-bounded organelle Source: RGD
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Cytochrome P450 4F1PRO_0000051849Add
BLAST

Proteomic databases

PaxDbiP33274.
PRIDEiP33274.

Expressioni

Developmental stagei

The level of P450 4F1 is found to increase dramatically in hepatic tumors.

Gene expression databases

GenevestigatoriP33274.

Interactioni

Protein-protein interaction databases

MINTiMINT-4569528.

Structurei

3D structure databases

ProteinModelPortaliP33274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP33274.
PhylomeDBiP33274.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33274-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQLSLSWLG LGPEVAFPWQ TLLLFGASWI LAQILTQIYA AYRNFRRLRG
60 70 80 90 100
FPQPPKRNWL MGHVGMVTPT EQGLKELTRL VGTYPQGFLM WIGPMVPVIT
110 120 130 140 150
LCHSDIVRSI LNASAAVALK DVIFYTILKP WLGDGLLVSA GDKWSRHRRM
160 170 180 190 200
LTPAFHFNIL KPYVKIFNDS TNIMHAKWKR LISEGSSRLD MFEHVSLMTL
210 220 230 240 250
DSLQKCVFSF DSNCQEKSSE YIAAILELSA LVAKRHQQPL LFMDLLYNLT
260 270 280 290 300
PDGMRFHKAC NLVHEFTDAV IRERRRTLPD QGLDEFLKSK AKSKTLDFID
310 320 330 340 350
VLLLTKDEDG KELSDEDIRA EADTFMFEGH DTTASGLSWI LYNLANDPEY
360 370 380 390 400
QERCRQEVQE LLRDRDPEEI EWDDLAQLPF LTMCIKESLR LHPPVTVISR
410 420 430 440 450
CCTQDILLPD GRTIPKGIIC LISIFGIHHN PSVWPDPEVY NPFRFDPENI
460 470 480 490 500
KDSSPLAFIP FSAGPRNCIG QTFAMSEMKV ALALTLLRFR LLPDDKEPRR
510 520
QPELILRAEG GLWLRVEPLT AGAQ
Length:524
Mass (Da):59,868
Last modified:February 1, 1994 - v1
Checksum:i572CEA2617CE632D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94548 mRNA. Translation: AAA41040.1.
AF200361 Genomic DNA. Translation: AAF20822.1.
PIRiS29723.
UniGeneiRn.5722.

Genome annotation databases

UCSCiRGD:70926. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94548 mRNA. Translation: AAA41040.1 .
AF200361 Genomic DNA. Translation: AAF20822.1 .
PIRi S29723.
UniGenei Rn.5722.

3D structure databases

ProteinModelPortali P33274.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4569528.

Proteomic databases

PaxDbi P33274.
PRIDEi P33274.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:70926. rat.

Organism-specific databases

RGDi 70926. Cyp4f1.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000233833.
HOVERGENi HBG000182.
InParanoidi P33274.
PhylomeDBi P33274.

Gene expression databases

Genevestigatori P33274.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of a new P450 subfamily, CYP4F1, expressed in rat hepatic tumors."
    Chen L., Hardwick J.P.
    Arch. Biochem. Biophys. 300:18-23(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Hepatoma.
  2. "Structure and transcriptional activity of the CYP4F1 leukotriene omega hydroxylase gene."
    Donelson E.L., Wu Y., Vernell R., Chen L., Hardwick J.P.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  3. "Covalent attachment of the heme prosthetic group in the CYP4F cytochrome P450 family."
    LeBrun L.A., Xu F., Kroetz D.L., Ortiz de Montellano P.R.
    Biochemistry 41:5931-5937(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: COVALENT HEME ATTACHMENT.

Entry informationi

Entry nameiCP4F1_RAT
AccessioniPrimary (citable) accession number: P33274
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 26, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3