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Protein

Cytochrome P450 2B12

Gene

Cyp2b12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. This isozyme seems responsible for metabolism of 2,2',4,4',5,5'-hexachlorobiphenyl.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi437 – 4371Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. arachidonic acid epoxygenase activity Source: RGD
  2. aromatase activity Source: UniProtKB-EC
  3. heme binding Source: GO_Central
  4. iron ion binding Source: InterPro
  5. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  6. oxygen binding Source: GO_Central
  7. steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  1. epoxygenase P450 pathway Source: RGD
  2. exogenous drug catabolic process Source: GO_Central
  3. oxidation-reduction process Source: GO_Central
  4. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2B12 (EC:1.14.14.1)
Alternative name(s):
CYPIIB12
Gene namesi
Name:Cyp2b12
Synonyms:Cyp2b-12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620088. Cyp2b12.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Cytochrome P450 2B12PRO_0000051687Add
BLAST

Proteomic databases

PaxDbiP33272.

Expressioni

Tissue specificityi

Preputial gland, but not in liver.

Gene expression databases

GenevestigatoriP33272.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000048784.

Structurei

3D structure databases

ProteinModelPortaliP33272.
SMRiP33272. Positions 29-492.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
KOiK07412.
PhylomeDBiP33272.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33272-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEFGVLLLLT LTVGFLLFLV SQSQPKTHGH LPPGPRPLPF LGNLLQMNRR
60 70 80 90 100
GFLNSFMQLQ EKYGDVFTVH LGPRPVVILC GTDTIREALV DQAEAFSGRG
110 120 130 140 150
TVAVLHPVVQ GYGVIFATGE RWKTLRRFSL VTMKEFGMGK RSVDERIKEE
160 170 180 190 200
AQCLVEELKK YKGAPLNPTF LFQSIAANTI CSIVFGERFD YKDHQFLHLL
210 220 230 240 250
DLVYKTSVLM GSLSSQVFEL YSGFLKYFPG AHKQIFKNLQ EMLNYIGHIV
260 270 280 290 300
EKHRATLDPS APRDFIDTYL LRMEKEKSNH HTEFNHQNLV ISVLSLFFAG
310 320 330 340 350
TETTSTTLRC TFLIMLKYPH VAEKVQKEID QVIGSHRLPT PDDRTKMPYT
360 370 380 390 400
DAVIHEIQRF ADLTPIGLPH RVTKDTVFRG YLLPKNTEVY PILSSALHDP
410 420 430 440 450
RYFEQPDTFN PEHFLDANGA LKKSEAFLPF STGKRICLGE GIARNELFIF
460 470 480 490
FTAILQNFTL ASPVAPEDID LTPINIGVGK IPSPYQINFL SR
Length:492
Mass (Da):55,797
Last modified:February 1, 1994 - v1
Checksum:iA21B1D4F9D77445B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63545 mRNA. Translation: CAA45107.1.
PIRiS27160.
RefSeqiNP_058852.1. NM_017156.1.
UniGeneiRn.144570.

Genome annotation databases

GeneIDi29295.
KEGGirno:29295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63545 mRNA. Translation: CAA45107.1.
PIRiS27160.
RefSeqiNP_058852.1. NM_017156.1.
UniGeneiRn.144570.

3D structure databases

ProteinModelPortaliP33272.
SMRiP33272. Positions 29-492.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000048784.

Proteomic databases

PaxDbiP33272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29295.
KEGGirno:29295.

Organism-specific databases

CTDi29295.
RGDi620088. Cyp2b12.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
KOiK07412.
PhylomeDBiP33272.

Miscellaneous databases

NextBioi608695.

Gene expression databases

GenevestigatoriP33272.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of a novel cytochrome CYP2B cDNA coding for a protein which is expressed in a sebaceous gland, but not in the liver."
    Friedberg T., Grassow M.A., Bartlomowicz-Oesch B., Siegert P., Arand M., Adesnik M., Oesch F.
    Biochem. J. 287:775-783(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiCP2BC_RAT
AccessioniPrimary (citable) accession number: P33272
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.