P33269 (CP4D1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 4d1
EC=1.14.-.-

Alternative name(s):
CYPIVD1

Gene names

Name:	Cyp4d1
Synonyms:	CYT-P450-D1
ORF Names:	CG3656

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Developmental stage	Expressed throughout development, with the highest levels occurring during late larval stages, then falling drastically during pupariation. Ref.1
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lipid particle Inferred from direct assay PubMed 16543254. Source: FlyBase
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P33269-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P33269-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-186: MFLVIGAILA...DWINLCTMDT → MWLLLSLVLL...EAIHLCALDA

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Cytochrome P450 4d1		PRO_0000051832
Sites
	Metal binding	456	1	Iron (heme axial ligand) By similarity
	Binding site	316	1	Heme (covalent; via 1 link) By similarity
Natural variations
	Alternative sequence	1 – 186	186	MFLVI…CTMDT → MWLLLSLVLLLAIIALEMRR FLRNMRTIPGPLPLPLLGNA HIFLGLTPAEACLKIGELAE RHGDTFGLFLGPSYSVMLFN PRDVERVLGSSQLLTKSQEY SFLGRWLNEGLLVSNGRKWH RRRKIITPAFHFRILEPYVE IFDRQSLRLVEELALRISRG QERINLGEAIHLCALDA in isoform Short.		VSP_000614
	Natural variant	170	1	E → D in strain: CAM-2, CAM-3, CAM-8, CAM-12, CAM-41, CAM-44, CAM-48 and Berkeley.
	Natural variant	426	1	S → I in strain: CAM-8, CAM-44, CAM-48 and Berkeley.
Experimental info
	Sequence conflict	68	1	Y → I in CAA47887. Ref.1
	Sequence conflict	316	1	E → K in CAA47887. Ref.1
	Sequence conflict	468 – 509	42	AIVAN…FKVRE → PSWPMCSGTTRLTLWATSFG TTRADRRTYSAYQGPLSSRC G in CAA47887. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified December 15, 1998. Version 2. Checksum: F7B089734231A6A3	FASTA	512	58,691
	10 20 30 40 50 60 MFLVIGAILA SALFVGLLLY HLKFKRLIDL ISYMPGPPVL PLVGHGHHFI GKPPHEMVKK 70 80 90 100 110 120 IFEFMETYSK DQVLKVWLGP ELNVLMGNPK DVEVVLGTLR FNDKAGEYKA LEPWLKEGLL 130 140 150 160 170 180 VSRGRKWHKR RKIITPAFHF KILDQFVEVF EKGSRDLLRN MEQDRLKHGE SGFSLYDWIN 190 200 210 220 230 240 LCTMDTICET AMGVSINAQS NADSEYVQAV KTISMVLHKR MFNILYRFDL TYMLTPLARA 250 260 270 280 290 300 EKKALNVLHQ FTEKIIVQRR EELIREGSSQ ESSNDDADVG AKRKMAFLDI LLQSTVDERP 310 320 330 340 350 360 LSNLDIREEV DTFMFEGHDT TSSALMFFFY NIATHPEAQK KCFEEIRSVV GNDKSTPVSY 370 380 390 400 410 420 ELLNQLHYVD LCVKETLRMY PSVPLLGRKV LEDCEINGKL IPAGTNIGIS PLYLGRREEL 430 440 450 460 470 480 FSEPNSFKPE RFDVVTTAEK LNPYAYIPFS AGPRNCIGQK FAMLEIKAIV ANVLRHYEVD 490 500 510 FVGDSSEPPV LIAELILRTK EPLMFKVRER VY
	Isoform Short [UniParc]. Checksum: 0F5E158E24D43DCE	FASTA	503	57,414
	10 20 30 40 50 60 MWLLLSLVLL LAIIALEMRR FLRNMRTIPG PLPLPLLGNA HIFLGLTPAE ACLKIGELAE 70 80 90 100 110 120 RHGDTFGLFL GPSYSVMLFN PRDVERVLGS SQLLTKSQEY SFLGRWLNEG LLVSNGRKWH 130 140 150 160 170 180 RRRKIITPAF HFRILEPYVE IFDRQSLRLV EELALRISRG QERINLGEAI HLCALDAICE 190 200 210 220 230 240 TAMGVSINAQ SNADSEYVQA VKTISMVLHK RMFNILYRFD LTYMLTPLAR AEKKALNVLH 250 260 270 280 290 300 QFTEKIIVQR REELIREGSS QESSNDDADV GAKRKMAFLD ILLQSTVDER PLSNLDIREE 310 320 330 340 350 360 VDTFMFEGHD TTSSALMFFF YNIATHPEAQ KKCFEEIRSV VGNDKSTPVS YELLNQLHYV 370 380 390 400 410 420 DLCVKETLRM YPSVPLLGRK VLEDCEINGK LIPAGTNIGI SPLYLGRREE LFSEPNSFKP 430 440 450 460 470 480 ERFDVVTTAE KLNPYAYIPF SAGPRNCIGQ KFAMLEIKAI VANVLRHYEV DFVGDSSEPP 490 500 VLIAELILRT KEPLMFKVRE RVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of a cytochrome P450 gene of family 4 on the Drosophila X chromosome." Gandhi R., Varak E., Goldberg M.L. DNA Cell Biol. 11:397-404(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Strain: Oregon-R. Tissue: Embryo.
[2]	"Evidence for non-neutral evolution around the cytochrome p450 gene cluster on the Drosophila melanogaster X chromosome." Phillips K.S., Begun D.J., Aquadro C.F. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CAM-1, CAM-12, CAM-14, CAM-19, CAM-2, CAM-3, CAM-38, CAM-41, CAM-42, CAM-44, CAM-48, CAM-8 and CAM-9.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	"From sequence to chromosome: the tip of the X chromosome of D. melanogaster." Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G., Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Borkova D., Minana B. , Kafatos F.C., Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L., Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P., Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H., Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S., McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C., Glover D.M. Science 287:2220-2222(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Oregon-R.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67645 mRNA. Translation: CAA47887.1. AF016992 Genomic DNA. Translation: AAB71155.1. AF016993 Genomic DNA. Translation: AAB71156.1. AF016994 Genomic DNA. Translation: AAB71157.1. AF016995 Genomic DNA. Translation: AAB71158.1. AF016996 Genomic DNA. Translation: AAB71159.1. AF016997 Genomic DNA. Translation: AAB71160.1. AF016998 Genomic DNA. Translation: AAB71161.1. AF016999 Genomic DNA. Translation: AAB71162.1. AF017000 Genomic DNA. Translation: AAB71163.1. AF017001 Genomic DNA. Translation: AAB71164.1. AF017002 Genomic DNA. Translation: AAB71165.1. AF017003 Genomic DNA. Translation: AAB71166.1. AF017004 Genomic DNA. Translation: AAB71167.1. AE014298 Genomic DNA. Translation: AAF45736.1. AE014298 Genomic DNA. Translation: AAF45737.1. Z98269 Genomic DNA. Translation: CAB10972.1.
PIR	S25707. T13611.
RefSeq	NP_476907.2. NM_057559.3. NP_726797.1. NM_166932.1.
UniGene	Dm.4852.
3D structure databases
ProteinModelPortal	P33269.
SMR	P33269. Positions 73-510.
ModBase	Search...
Proteomic databases
PaxDb	P33269.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	31188.
KEGG	dme:Dmel_CG3656.
Organism-specific databases
CTD	31188.
FlyBase	FBgn0005670. Cyp4d1.
Phylogenomic databases
eggNOG	COG2124.
InParanoid	P33269.
KO	K15001.
OrthoDB	EOG4573NP.
Gene expression databases
GermOnline	CG3656. Drosophila melanogaster.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Cyp4d1. drosophila.
GenomeRNAi	31188.
NextBio	772371.

Entry information

Entry name

CP4D1_DROME

Accession

Primary (citable) accession number: P33269
Secondary accession number(s): O18644 , O18653, O18664, Q9W515, Q9W516

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project