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Protein

Cytochrome P450 4d1

Gene

Cyp4d1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei316 – 3161Heme (covalent; via 1 link)By similarity
Metal bindingi456 – 4561Iron (heme axial ligand)By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4d1 (EC:1.14.-.-)
Alternative name(s):
CYPIVD1
Gene namesi
Name:Cyp4d1
Synonyms:CYT-P450-D1
ORF Names:CG3656
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0005670. Cyp4d1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • lipid particle Source: FlyBase
  • membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Cytochrome P450 4d1PRO_0000051832Add
BLAST

Proteomic databases

PaxDbiP33269.
PeptideAtlasiP33269.
PRIDEiP33269.

Expressioni

Developmental stagei

Expressed throughout development, with the highest levels occurring during late larval stages, then falling drastically during pupariation.1 Publication

Gene expression databases

BgeeiFBgn0005670.
ExpressionAtlasiP33269. differential.
GenevisibleiP33269. DM.

Interactioni

Protein-protein interaction databases

IntActiP33269. 1 interaction.
STRINGi7227.FBpp0070412.

Structurei

3D structure databases

ProteinModelPortaliP33269.
SMRiP33269. Positions 73-510.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
InParanoidiP33269.
KOiK15001.
OrthoDBiEOG091G06KN.
PhylomeDBiP33269.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P33269-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFLVIGAILA SALFVGLLLY HLKFKRLIDL ISYMPGPPVL PLVGHGHHFI
60 70 80 90 100
GKPPHEMVKK IFEFMETYSK DQVLKVWLGP ELNVLMGNPK DVEVVLGTLR
110 120 130 140 150
FNDKAGEYKA LEPWLKEGLL VSRGRKWHKR RKIITPAFHF KILDQFVEVF
160 170 180 190 200
EKGSRDLLRN MEQDRLKHGE SGFSLYDWIN LCTMDTICET AMGVSINAQS
210 220 230 240 250
NADSEYVQAV KTISMVLHKR MFNILYRFDL TYMLTPLARA EKKALNVLHQ
260 270 280 290 300
FTEKIIVQRR EELIREGSSQ ESSNDDADVG AKRKMAFLDI LLQSTVDERP
310 320 330 340 350
LSNLDIREEV DTFMFEGHDT TSSALMFFFY NIATHPEAQK KCFEEIRSVV
360 370 380 390 400
GNDKSTPVSY ELLNQLHYVD LCVKETLRMY PSVPLLGRKV LEDCEINGKL
410 420 430 440 450
IPAGTNIGIS PLYLGRREEL FSEPNSFKPE RFDVVTTAEK LNPYAYIPFS
460 470 480 490 500
AGPRNCIGQK FAMLEIKAIV ANVLRHYEVD FVGDSSEPPV LIAELILRTK
510
EPLMFKVRER VY
Length:512
Mass (Da):58,691
Last modified:December 15, 1998 - v2
Checksum:iF7B089734231A6A3
GO
Isoform Short (identifier: P33269-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-186: MFLVIGAILA...DWINLCTMDT → MWLLLSLVLL...EAIHLCALDA

Note: No experimental confirmation available.
Show »
Length:503
Mass (Da):57,414
Checksum:i0F5E158E24D43DCE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681Y → I in CAA47887 (PubMed:1605861).Curated
Sequence conflicti316 – 3161E → K in CAA47887 (PubMed:1605861).Curated
Sequence conflicti468 – 50942AIVAN…FKVRE → PSWPMCSGTTRLTLWATSFG TTRADRRTYSAYQGPLSSRC G in CAA47887 (PubMed:1605861).CuratedAdd
BLAST

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti170 – 1701E → D in strain: CAM-2, CAM-3, CAM-8, CAM-12, CAM-41, CAM-44, CAM-48 and Berkeley.
Natural varianti426 – 4261S → I in strain: CAM-8, CAM-44, CAM-48 and Berkeley.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 186186MFLVI…CTMDT → MWLLLSLVLLLAIIALEMRR FLRNMRTIPGPLPLPLLGNA HIFLGLTPAEACLKIGELAE RHGDTFGLFLGPSYSVMLFN PRDVERVLGSSQLLTKSQEY SFLGRWLNEGLLVSNGRKWH RRRKIITPAFHFRILEPYVE IFDRQSLRLVEELALRISRG QERINLGEAIHLCALDA in isoform Short. CuratedVSP_000614Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67645 mRNA. Translation: CAA47887.1.
AF016992 Genomic DNA. Translation: AAB71155.1.
AF016993 Genomic DNA. Translation: AAB71156.1.
AF016994 Genomic DNA. Translation: AAB71157.1.
AF016995 Genomic DNA. Translation: AAB71158.1.
AF016996 Genomic DNA. Translation: AAB71159.1.
AF016997 Genomic DNA. Translation: AAB71160.1.
AF016998 Genomic DNA. Translation: AAB71161.1.
AF016999 Genomic DNA. Translation: AAB71162.1.
AF017000 Genomic DNA. Translation: AAB71163.1.
AF017001 Genomic DNA. Translation: AAB71164.1.
AF017002 Genomic DNA. Translation: AAB71165.1.
AF017003 Genomic DNA. Translation: AAB71166.1.
AF017004 Genomic DNA. Translation: AAB71167.1.
AE014298 Genomic DNA. Translation: AAF45736.1.
AE014298 Genomic DNA. Translation: AAF45737.1.
Z98269 Genomic DNA. Translation: CAB10972.1.
PIRiS25707.
T13611.
RefSeqiNP_476907.2. NM_057559.4.
NP_726797.1. NM_166932.2.
UniGeneiDm.4852.

Genome annotation databases

GeneIDi31188.
KEGGidme:Dmel_CG3656.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67645 mRNA. Translation: CAA47887.1.
AF016992 Genomic DNA. Translation: AAB71155.1.
AF016993 Genomic DNA. Translation: AAB71156.1.
AF016994 Genomic DNA. Translation: AAB71157.1.
AF016995 Genomic DNA. Translation: AAB71158.1.
AF016996 Genomic DNA. Translation: AAB71159.1.
AF016997 Genomic DNA. Translation: AAB71160.1.
AF016998 Genomic DNA. Translation: AAB71161.1.
AF016999 Genomic DNA. Translation: AAB71162.1.
AF017000 Genomic DNA. Translation: AAB71163.1.
AF017001 Genomic DNA. Translation: AAB71164.1.
AF017002 Genomic DNA. Translation: AAB71165.1.
AF017003 Genomic DNA. Translation: AAB71166.1.
AF017004 Genomic DNA. Translation: AAB71167.1.
AE014298 Genomic DNA. Translation: AAF45736.1.
AE014298 Genomic DNA. Translation: AAF45737.1.
Z98269 Genomic DNA. Translation: CAB10972.1.
PIRiS25707.
T13611.
RefSeqiNP_476907.2. NM_057559.4.
NP_726797.1. NM_166932.2.
UniGeneiDm.4852.

3D structure databases

ProteinModelPortaliP33269.
SMRiP33269. Positions 73-510.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP33269. 1 interaction.
STRINGi7227.FBpp0070412.

Proteomic databases

PaxDbiP33269.
PeptideAtlasiP33269.
PRIDEiP33269.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi31188.
KEGGidme:Dmel_CG3656.

Organism-specific databases

CTDi31188.
FlyBaseiFBgn0005670. Cyp4d1.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
InParanoidiP33269.
KOiK15001.
OrthoDBiEOG091G06KN.
PhylomeDBiP33269.

Miscellaneous databases

ChiTaRSiCyp4d1. fly.
GenomeRNAii31188.
PROiP33269.

Gene expression databases

BgeeiFBgn0005670.
ExpressionAtlasiP33269. differential.
GenevisibleiP33269. DM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP4D1_DROME
AccessioniPrimary (citable) accession number: P33269
Secondary accession number(s): O18644
, O18653, O18664, Q9W515, Q9W516
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 15, 1998
Last modified: September 7, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.