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Protein

Cytochrome P450 3A8

Gene

CYP3A8

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes nifedipine and nilvadipine oxidations.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi442 – 4421Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. monooxygenase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 3A8 (EC:1.14.14.1)
Alternative name(s):
CYPIIIA8
Cytochrome P-450-MK2
Cytochrome P450-MKNF2
Gene namesi
Name:CYP3A8
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Cytochrome P450 3A8PRO_0000051791Add
BLAST

Proteomic databases

PRIDEiP33268.

Expressioni

Inductioni

By polychlorinated biphenyl (PCB).

Structurei

3D structure databases

ProteinModelPortaliP33268.
SMRiP33268. Positions 30-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG108567.
KOiK17689.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR008072. Cyt_P450_E_CYP3A.
IPR002402. Cyt_P450_E_grp-II.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00464. EP450II.
PR01689. EP450IICYP3A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLIPDLAVE TWLLLAVTLV LLYLYGTHSH GLFKKLGIPG PTPLPLLGNI
60 70 80 90 100
LSYRKGFWTF DMECYKKYGK VWGFYDGRQP VLAITDPNMI KTVLVKECYS
110 120 130 140 150
VFTNRRPFGP VGFMKNAISI AEDEEWKRIR SLLSPTFTSG KLKEMVPIIA
160 170 180 190 200
KYGDVLVRNL RREAETGKPV TLKDVFGAYS MDVITSTSFG VNIDSLNNPQ
210 220 230 240 250
DPFVENTKKL LRFDFLDPFF LSITIFPFII PILEVLNISI FPREVTSFLR
260 270 280 290 300
KSVKRIKESR LKDTQKHRVD FLQLMIDSQN SKETESHKAL SDLELVAQSI
310 320 330 340 350
IFIFAGYETT SSVLSFIIYE LATHPDVQQK LQEEIDTVLP NKAPPTYDTV
360 370 380 390 400
LQMEYLDMVV NETLRIFPIA MRLERVCKKD VEINGIFIPK GVVVMIPSYA
410 420 430 440 450
LHHDPKYWPE PEKFLPERFS KKNNDNIDPY IYTPFGSGPR NCIGMRFALM
460 470 480 490 500
NMKLAIIRVL QNFSFKPCKE TQIPLKLRLG GLLQTEKPIV LKIESRDGTV

SGA
Length:503
Mass (Da):57,511
Last modified:January 31, 1994 - v1
Checksum:iD701B6FE83AC8BFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53047 mRNA. Translation: AAB24952.1.
PIRiS28168.
RefSeqiNP_001271463.1. NM_001284534.1.
UniGeneiMfa.4832.

Genome annotation databases

GeneIDi102144258.
KEGGimcf:102144258.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53047 mRNA. Translation: AAB24952.1.
PIRiS28168.
RefSeqiNP_001271463.1. NM_001284534.1.
UniGeneiMfa.4832.

3D structure databases

ProteinModelPortaliP33268.
SMRiP33268. Positions 30-496.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP33268.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi102144258.
KEGGimcf:102144258.

Phylogenomic databases

HOVERGENiHBG108567.
KOiK17689.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR008072. Cyt_P450_E_CYP3A.
IPR002402. Cyt_P450_E_grp-II.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00464. EP450II.
PR01689. EP450IICYP3A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of monkey liver cytochrome P-450 cDNAs: similarity of the primary sequences to human cytochromes P-450."
    Komori M., Kikuchi O., Sakuma T., Funaki J., Kitada M., Kamataki T.
    Biochim. Biophys. Acta 1171:141-146(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Purification of cytochrome P-450 from polychlorinated biphenyl-treated crab-eating monkeys: high homology to a form of human cytochrome P-450."
    Ohta K., Kitada M., Hashizume T., Komori M., Ohi H., Kamataki T.
    Biochim. Biophys. Acta 996:142-145(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-22.
    Tissue: Liver.
  3. "Purification and characterization of two forms of hepatic microsomal cytochrome P450 from untreated cynomolgus monkeys."
    Ohmori S., Horie T., Guengerich F.P., Kiuchi M., Kitada M.
    Arch. Biochem. Biophys. 305:405-413(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiCP3A8_MACFA
AccessioniPrimary (citable) accession number: P33268
Secondary accession number(s): P25231
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1994
Last sequence update: January 31, 1994
Last modified: January 6, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.