P33268 (CP3A8_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 3A8
EC=1.14.14.1

Alternative name(s):
CYPIIIA8
Cytochrome P-450-MK2
Cytochrome P450-MKNF2

Gene names

Name:

CYP3A8

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes nifedipine and nilvadipine oxidations.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By polychlorinated biphenyl (PCB).
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Cytochrome P450 3A8		PRO_0000051791
Sites
	Metal binding	442	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33268 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: D701B6FE83AC8BFB	FASTA	503	57,511
	10 20 30 40 50 60 MDLIPDLAVE TWLLLAVTLV LLYLYGTHSH GLFKKLGIPG PTPLPLLGNI LSYRKGFWTF 70 80 90 100 110 120 DMECYKKYGK VWGFYDGRQP VLAITDPNMI KTVLVKECYS VFTNRRPFGP VGFMKNAISI 130 140 150 160 170 180 AEDEEWKRIR SLLSPTFTSG KLKEMVPIIA KYGDVLVRNL RREAETGKPV TLKDVFGAYS 190 200 210 220 230 240 MDVITSTSFG VNIDSLNNPQ DPFVENTKKL LRFDFLDPFF LSITIFPFII PILEVLNISI 250 260 270 280 290 300 FPREVTSFLR KSVKRIKESR LKDTQKHRVD FLQLMIDSQN SKETESHKAL SDLELVAQSI 310 320 330 340 350 360 IFIFAGYETT SSVLSFIIYE LATHPDVQQK LQEEIDTVLP NKAPPTYDTV LQMEYLDMVV 370 380 390 400 410 420 NETLRIFPIA MRLERVCKKD VEINGIFIPK GVVVMIPSYA LHHDPKYWPE PEKFLPERFS 430 440 450 460 470 480 KKNNDNIDPY IYTPFGSGPR NCIGMRFALM NMKLAIIRVL QNFSFKPCKE TQIPLKLRLG 490 500 GLLQTEKPIV LKIESRDGTV SGA

References

[1]	"Molecular cloning of monkey liver cytochrome P-450 cDNAs: similarity of the primary sequences to human cytochromes P-450." Komori M., Kikuchi O., Sakuma T., Funaki J., Kitada M., Kamataki T. Biochim. Biophys. Acta 1171:141-146(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Purification of cytochrome P-450 from polychlorinated biphenyl-treated crab-eating monkeys: high homology to a form of human cytochrome P-450." Ohta K., Kitada M., Hashizume T., Komori M., Ohi H., Kamataki T. Biochim. Biophys. Acta 996:142-145(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-22. Tissue: Liver.
[3]	"Purification and characterization of two forms of hepatic microsomal cytochrome P450 from untreated cynomolgus monkeys." Ohmori S., Horie T., Guengerich F.P., Kiuchi M., Kitada M. Arch. Biochem. Biophys. 305:405-413(1993) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S53047 mRNA. Translation: AAB24952.1.
PIR	S28168.
3D structure databases
ProteinModelPortal	P33268.
SMR	P33268. Positions 30-496.
ModBase	Search...
Proteomic databases
PRIDE	P33268.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG108567.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR008072. Cyt_P450_E_CYP3A. IPR002402. Cyt_P450_E_grp-II. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00464. EP450II. PR01689. EP450IICYP3A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP3A8_MACFA

Accession

Primary (citable) accession number: P33268
Secondary accession number(s): P25231

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program