P33265 (CP2CS_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2C28
EC=1.14.14.1

Alternative name(s):
CYPIIC28
Cytochrome P450 HSM4

Gene names

Name:

CYP2C28

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the N-demethylation of aminopyrine and benzphetamine, but does not catalyze the hydroxylation of tolbutamide, testosterone, and progesterone.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Cytochrome P450 2C28		PRO_0000051716
Sites
	Metal binding	435	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33265 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: DD567183B3A51801	FASTA	490	55,703
	10 20 30 40 50 60 MDPVLVLVLT LSCLLLFSVW RQSSGRGRLP PGPTPLPLIG NILQIDIKDI SKSLANFSKV 70 80 90 100 110 120 YGPVFTLYFG TKPTVVVHGY EAVKEALEDL GEEFSGRGNF PIVERMNSGL GIIFSNGTKW 130 140 150 160 170 180 KELRRFSLMT LRNFGMGKRS IEDCIQEEAR CLVEELRKTN GSPCDPTFFL SCAPSNVICS 190 200 210 220 230 240 VVFHNRFDYN DKNFLNLMEK LNENFEILNS PWLQVCNVIP AFLDYLPGSH NKALKNFAEI 250 260 270 280 290 300 KSYILKRVKE HQETLDMNNP RDFIDCFLIK MEKEKDNPHS EFTTESLMAT VADVFVAGSE 310 320 330 340 350 360 TTSTTLRYGL LLLLKHKEVT AKVQKEIDHV IGRDRSPCMQ DRTRMPYTDA MVHEVQRYVN 370 380 390 400 410 420 LIPNNVPHAA TCNVKFRNYV IPKGTDLITS LTSVLHDDKE FPNPKIFDPA HFLDENGNFK 430 440 450 460 470 480 KSDYFMPFSI GKRMCMGEAL ARMELFLLLT TILQNFDLKS LADTKDIDTT PVASTFGCVP 490 PSYQLYFIPR

References

[1]	"Isolation and characterization of a new cDNA clone belonging to the cytochrome P450 2C gene subfamily in hamsters." Sakuma T., Yokoi T., Kamataki T. Arch. Biochem. Biophys. 319:267-273(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11437 mRNA. Translation: BAA02003.1.
PIR	I48164.
3D structure databases
ProteinModelPortal	P33265.
SMR	P33265. Positions 30-489.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG015789.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2CS_MESAU

Accession

Primary (citable) accession number: P33265

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program