ID   CP2CR_MESAU             Reviewed;         490 AA.
AC   P33264;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-MAY-2023, entry version 110.
DE   RecName: Full=Cytochrome P450 2C27;
DE            EC=1.14.14.1;
DE   AltName: Full=CYPIIC27;
DE   AltName: Full=Cytochrome P450 HSM3;
GN   Name=CYP2C27;
OS   Mesocricetus auratus (Golden hamster).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC   Cricetidae; Cricetinae; Mesocricetus.
OX   NCBI_TaxID=10036;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   PubMed=8114672;
RA   Sakuma T., Masaki K., Itoh S., Yokoi T., Kamataki T.;
RT   "Sex-related differences in the expression of cytochrome P450 in hamsters:
RT   cDNA cloning and examination of the expression of three distinct CYP2C
RT   cDNAs.";
RL   Mol. Pharmacol. 45:228-236(1994).
CC   -!- FUNCTION: Catalyzes the hydroxylation of tolbutamide and the N-
CC       demethylation of aminopyrine and benzphetamine.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC         reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC         reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC         COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:142491; EC=1.14.14.1;
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC       membrane protein. Microsome membrane; Peripheral membrane protein.
CC   -!- TISSUE SPECIFICITY: Specifically expressed by males in kidneys.
CC       Expressed predominantly by males in livers.
CC   -!- INDUCTION: P450 can be induced to high levels in liver and other
CC       tissues by various foreign compounds, including drugs, pesticides, and
CC       carcinogens.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; D11436; BAA02002.1; -; mRNA.
DR   PIR; I48163; I48163.
DR   RefSeq; NP_001268504.1; NM_001281575.1.
DR   AlphaFoldDB; P33264; -.
DR   SMR; P33264; -.
DR   GeneID; 101841752; -.
DR   KEGG; ag:BAA02002; -.
DR   KEGG; maua:101841752; -.
DR   OrthoDB; 2900138at2759; -.
DR   Proteomes; UP000189706; Unplaced.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   CDD; cd20665; CYP2C-like; 1.
DR   Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   PANTHER; PTHR24300:SF374; CYTOCHROME P450 2C18; 1.
DR   PANTHER; PTHR24300; CYTOCHROME P450 508A4-RELATED; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; Cytochrome P450; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW   Monooxygenase; Oxidoreductase; Reference proteome.
FT   CHAIN           1..490
FT                   /note="Cytochrome P450 2C27"
FT                   /id="PRO_0000051715"
FT   BINDING         435
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   490 AA;  55767 MW;  450A208070D60D0A CRC64;
     MDAFVVLVFI LSCLFLLSLW RQSSERGKLP PGPTPLPLIG NFFQIDVKDI SGSLTNFSKV
     YGPVFTLYLG RKPAVVLHGY EAVKEALIDH GEEFAGRGSF PMAERYNKGL GIVFSNGNRW
     KDIRRFSLMA LRSLGMGKRS IEDRVQEEAQ CLVEELRKTN GSPCDPTFIL SCAPCNVICS
     IIFQNRFDYT DQDFLTFMEK VNENVRILSS PWLQVCNNFP SLIDYCPGSH HTITKNVNYI
     RSYLSEKIKE HQETLDVANP RDFIDYYLIK LKQGNYNQQS EFTLENLATT VRDLFAAGTE
     TTSTTLRYAL LLLLKHPHVT AKVQEEIDQV VGRHRNPCMQ DRSHMPYTDA MIHEVQRFID
     LIPTNLPHAV TCDIKFRDYF IPKGTTIITS LSSVLHDSKE FPNPEVFDPG HFLDKNGKFK
     KSDYFMPFST GKRMCAGEGL ARMELFLFLT TILQNFKLKS LVHPKDIDTT PVVNGLASLP
     PSYQLCFIPV
//