P33262 (CP2CK_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2C20
EC=1.14.14.1

Alternative name(s):
CYPIIC20
Cytochrome P450-MK1
Cytochrome P450-MKMP13

Gene names

Name:

CYP2C20

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By 3-methylcholanthrene (3MC).
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Cytochrome P450 2C20		PRO_0000051709
Sites
	Metal binding	435	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33262 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 7CEC7078C2F25990	FASTA	490	55,638
	10 20 30 40 50 60 MDPFVVLVLC LSFVLLFSLW RQSSGRRKLP PGPTPLPIIG NILQIDVKDI CKSFSNFSKV 70 80 90 100 110 120 YGPVFTVYFG MNPVVVLHGY ETVKEALIDN AEEFSGRGIL PISERITNGL GIISSNGKRW 130 140 150 160 170 180 KETRRFSLTT LRNFGMGKRS IEDRVQEEAR CLVEELRKTK ASPCDPTFIL GCAPCNVICS 190 200 210 220 230 240 VVFQKRFDYK DENFLTLIKR FTVNFRILTS PWIQVCNNFP LLIDCFPGTH NKLLKNVALT 250 260 270 280 290 300 KSYIREKVKE HQATLDVNNP RDFIDCFLIK MEQEKDNQQS EFTIENLVGT VADLFVAGTE 310 320 330 340 350 360 TTSTTLRYGL LLLLKHPEVT AKVQEEIDHV IGRHRSPCMQ DRSHMPYTDA VIHEIQRYID 370 380 390 400 410 420 LVPTGVPHAV TTDIKFRNYL IPKGTIIITL LTSVLHDDKE FPNPKIFDPG HFLDENGNFK 430 440 450 460 470 480 KSDYFMPFSA GKRICAGEGL ARMELFLFLT TILQNFNLKS VADLKNLNTT SATRGIISLP 490 PSYQICFIPV

References

[1]

"Molecular cloning of monkey liver cytochrome P-450 cDNAs: similarity of the primary sequences to human cytochromes P-450." Komori M., Kikuchi O., Sakuma T., Funaki J., Kitada M., Kamataki T. Biochim. Biophys. Acta 1171:141-146(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S53046 mRNA. Translation: AAB24950.1.
PIR	S28166.
3D structure databases
ProteinModelPortal	P33262.
SMR	P33262. Positions 28-490.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG015789.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2CK_MACFA

Accession

Primary (citable) accession number: P33262

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program