P33260 (CP2CI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2C18
EC=1.14.14.1

Alternative name(s):
CYPIIC18
Cytochrome P450-6b/29c

Gene names

Name:

CYP2C18

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	small molecule metabolic process Traceable author statement. Source: Reactome xenobiotic metabolic process Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Traceable author statement PubMed 8110777. Source: ProtInc oxygen binding Traceable author statement PubMed 8110777. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P33260-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P33260-2)

The sequence of this isoform differs from the canonical sequence as follows:
     214-272: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Cytochrome P450 2C18		PRO_0000051707
Sites
	Metal binding	435	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	214 – 272	59	Missing in isoform 2.		VSP_042520
	Natural variant	385	1	T → M. Ref.1 Ref.3 Corresponds to variant rs2281891 [ dbSNP | Ensembl ].		VAR_001254

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 7, 2005. Version 3. Checksum: C933CF31F078FD1E	FASTA	490	55,711
	10 20 30 40 50 60 MDPAVALVLC LSCLFLLSLW RQSSGRGRLP SGPTPLPIIG NILQLDVKDM SKSLTNFSKV 70 80 90 100 110 120 YGPVFTVYFG LKPIVVLHGY EAVKEALIDH GEEFSGRGSF PVAEKVNKGL GILFSNGKRW 130 140 150 160 170 180 KEIRRFCLMT LRNFGMGKRS IEDRVQEEAR CLVEELRKTN ASPCDPTFIL GCAPCNVICS 190 200 210 220 230 240 VIFHDRFDYK DQRFLNLMEK FNENLRILSS PWIQVCNNFP ALIDYLPGSH NKIAENFAYI 250 260 270 280 290 300 KSYVLERIKE HQESLDMNSA RDFIDCFLIK MEQEKHNQQS EFTVESLIAT VTDMFGAGTE 310 320 330 340 350 360 TTSTTLRYGL LLLLKYPEVT AKVQEEIECV VGRNRSPCMQ DRSHMPYTDA VVHEIQRYID 370 380 390 400 410 420 LLPTNLPHAV TCDVKFKNYL IPKGTTIITS LTSVLHNDKE FPNPEMFDPG HFLDKSGNFK 430 440 450 460 470 480 KSDYFMPFSA GKRMCMGEGL ARMELFLFLT TILQNFNLKS QVDPKDIDIT PIANAFGRVP 490 PLYQLCFIPV
	Isoform 2 [UniParc]. Checksum: 0FF49ECE8F830455	FASTA	431	48,805
	10 20 30 40 50 60 MDPAVALVLC LSCLFLLSLW RQSSGRGRLP SGPTPLPIIG NILQLDVKDM SKSLTNFSKV 70 80 90 100 110 120 YGPVFTVYFG LKPIVVLHGY EAVKEALIDH GEEFSGRGSF PVAEKVNKGL GILFSNGKRW 130 140 150 160 170 180 KEIRRFCLMT LRNFGMGKRS IEDRVQEEAR CLVEELRKTN ASPCDPTFIL GCAPCNVICS 190 200 210 220 230 240 VIFHDRFDYK DQRFLNLMEK FNENLRILSS PWIQEKHNQQ SEFTVESLIA TVTDMFGAGT 250 260 270 280 290 300 ETTSTTLRYG LLLLLKYPEV TAKVQEEIEC VVGRNRSPCM QDRSHMPYTD AVVHEIQRYI 310 320 330 340 350 360 DLLPTNLPHA VTCDVKFKNY LIPKGTTIIT SLTSVLHNDK EFPNPEMFDP GHFLDKSGNF 370 380 390 400 410 420 KKSDYFMPFS AGKRMCMGEG LARMELFLFL TTILQNFNLK SQVDPKDIDI TPIANAFGRV 430 PPLYQLCFIP V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of complementary DNAs for multiple members of the human cytochrome P450IIC subfamily." Romkes M., Faletto M.B., Blaisdell J.A., Raucy J.L., Goldstein J.A. Biochemistry 30:3247-3255(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT MET-385. Tissue: Liver.
[2]	Erratum Romkes M., Faletto M.B., Blaisdell J.A., Raucy J.L., Goldstein J.A. Biochemistry 32:1390-1390(1993) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Gene structure and upstream regulatory regions of human CYP2C9 and CYP2C18." de Morais S.M., Schweikl H., Blaisdell J., Goldstein J.A. Biochem. Biophys. Res. Commun. 194:194-201(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT MET-385. Tissue: Liver.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Stomach.
[5]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[8]	"Alloreactivity between disparate cognate and allogeneic pMHC-I complexes is the result of highly focused, peptide-dependent structural mimicry." Archbold J.K., Macdonald W.A., Miles J.J., Brennan R.M., Kjer-Nielsen L., McCluskey J., Burrows S.R., Rossjohn J. J. Biol. Chem. 281:34324-34332(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 72-80.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M61853 mRNA. No translation available.
M61856 mRNA. Translation: AAB59356.1.
L16876 , L16871, L16872, L16869, L16870, L16875, L16873, L16874 Genomic DNA. Translation: AAA02630.1.
AK313403 mRNA. Translation: BAG36199.1.
AL583836 Genomic DNA. Translation: CAH74067.1.
CH471066 Genomic DNA. Translation: EAW50024.1.
BC069666 mRNA. Translation: AAH69666.1.
BC096257 mRNA. Translation: AAH96257.1.
BC096258 mRNA. Translation: AAH96258.1.
BC096260 mRNA. Translation: AAH96260.1.

IPI

IPI00289647.
IPI00844265.

PIR

A61269.

RefSeq

NP_000763.1. NM_000772.2.
NP_001122397.1. NM_001128925.1.

UniGene

Hs.511872.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2H6P	X-ray	1.90	C	72-80	[»]

ProteinModelPortal

P33260.

ModBase

Search...

Protein-protein interaction databases

IntAct

P33260. 3 interactions.

STRING

9606.ENSP00000285979.

PTM databases

PhosphoSite

P33260.

Polymorphism databases

DMDM

67476954.

Proteomic databases

PaxDb

P33260.

PRIDE

P33260.

Protocols and materials databases

DNASU

1562.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000285979; ENSP00000285979; ENSG00000108242.
ENST00000339022; ENSP00000341293; ENSG00000108242.

GeneID

1562.

KEGG

hsa:1562.

UCSC

uc001kjv.4. human.
uc001kjw.4. human.

Organism-specific databases

CTD

1562.

GeneCards

GC10P096433.

HGNC

HGNC:2620. CYP2C18.

MIM

601131. gene.

neXtProt

NX_P33260.

PharmGKB

PA127.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG2124.

HOGENOM

HOG000036992.

HOVERGEN

HBG015789.

InParanoid

P33260.

KO

K07413.

OMA

QVDPKDI.

OrthoDB

EOG48WC22.

PhylomeDB

P33260.

Enzyme and pathway databases

Pathway_Interaction_DB

hnf3apathway. FOXA1 transcription factor network.

Reactome

REACT_111217. Metabolism.

SABIO-RK

P33260.

Gene expression databases

ArrayExpress

P33260.

Bgee

P33260.

CleanEx

HS_CYP2C18.

Genevestigator

P33260.

GermOnline

ENSG00000108242. Homo sapiens.

Family and domain databases

Gene3D

1.10.630.10. 1 hit.

InterPro

IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]

Pfam

PF00067. p450. 1 hit.
[Graphical view]

PRINTS

PR00463. EP450I.
PR00385. P450.

SUPFAM

SSF48264. Cytochrome_P450. 1 hit.

PROSITE

PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P33260.

ChEMBL

CHEMBL2408.

EvolutionaryTrace

P33260.

GenomeRNAi

1562.

NextBio

6442.

SOURCE

Search...

Entry information

Entry name

CP2CI_HUMAN

Accession

Primary (citable) accession number: P33260
Secondary accession number(s): B2R8K2 , Q16703, Q16751, Q4VAT5, Q6GRG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	June 7, 2005
Last modified:	May 29, 2013
This is version 120 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.