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Protein

Squalene--hopene cyclase

Gene

shc

Organism
Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / JCM 5260 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-1A) (Bacillus acidocaldarius)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of squalene into hopene.

Catalytic activityi

Squalene = hop-22(29)-ene.
Hopan-22-ol = squalene + H2O.

Pathwayi: hopanoid biosynthesis

This protein is involved in the pathway hopanoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway hopanoid biosynthesis and in Secondary metabolite biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17503.
BRENDAi5.4.99.17. 243.
SABIO-RKP33247.
UniPathwayiUPA00337.

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene--hopene cyclase (EC:4.2.1.129, EC:5.4.99.17)
Alternative name(s):
Squalene--hopanol cyclase
Gene namesi
Name:shc
Ordered Locus Names:Aaci_2443
OrganismiAlicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / JCM 5260 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-1A) (Bacillus acidocaldarius)
Taxonomic identifieri521098 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesAlicyclobacillaceaeAlicyclobacillus
Proteomesi
  • UP000001917 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3569.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000726502 – 631Squalene--hopene cyclaseAdd BLAST630

Interactioni

Protein-protein interaction databases

STRINGi521098.Aaci_2443.

Chemistry databases

BindingDBiP33247.

Structurei

Secondary structure

1631
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 25Combined sources16
Beta strandi28 – 30Combined sources3
Helixi41 – 52Combined sources12
Helixi58 – 71Combined sources14
Beta strandi79 – 81Combined sources3
Helixi88 – 101Combined sources14
Beta strandi105 – 107Combined sources3
Helixi108 – 119Combined sources12
Helixi123 – 125Combined sources3
Helixi128 – 136Combined sources9
Helixi142 – 144Combined sources3
Helixi150 – 154Combined sources5
Beta strandi157 – 159Combined sources3
Helixi163 – 165Combined sources3
Helixi168 – 183Combined sources16
Helixi191 – 193Combined sources3
Helixi196 – 199Combined sources4
Beta strandi210 – 212Combined sources3
Helixi216 – 230Combined sources15
Helixi237 – 251Combined sources15
Beta strandi256 – 258Combined sources3
Helixi262 – 274Combined sources13
Helixi281 – 288Combined sources8
Helixi289 – 293Combined sources5
Beta strandi294 – 296Combined sources3
Turni298 – 300Combined sources3
Beta strandi302 – 304Combined sources3
Helixi310 – 323Combined sources14
Helixi331 – 342Combined sources12
Helixi350 – 353Combined sources4
Beta strandi365 – 368Combined sources4
Helixi375 – 385Combined sources11
Helixi393 – 409Combined sources17
Beta strandi415 – 417Combined sources3
Beta strandi419 – 423Combined sources5
Helixi428 – 431Combined sources4
Beta strandi432 – 434Combined sources3
Beta strandi436 – 438Combined sources3
Helixi446 – 457Combined sources12
Turni458 – 460Combined sources3
Beta strandi463 – 465Combined sources3
Helixi466 – 478Combined sources13
Beta strandi488 – 492Combined sources5
Helixi493 – 506Combined sources14
Helixi514 – 525Combined sources12
Helixi537 – 540Combined sources4
Helixi543 – 545Combined sources3
Beta strandi549 – 551Combined sources3
Helixi553 – 565Combined sources13
Beta strandi569 – 571Combined sources3
Helixi572 – 584Combined sources13
Beta strandi587 – 589Combined sources3
Beta strandi598 – 601Combined sources4
Turni602 – 604Combined sources3
Beta strandi605 – 609Combined sources5
Helixi612 – 629Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GSZX-ray2.80A/B/C2-631[»]
1H35X-ray2.80A/B/C2-631[»]
1H36X-ray2.80A/B/C2-631[»]
1H37X-ray2.80A/B/C2-631[»]
1H39X-ray2.80A/B/C2-631[»]
1H3AX-ray2.85A/B/C2-631[»]
1H3BX-ray2.80A/B/C2-631[»]
1H3CX-ray2.90A/B/C2-631[»]
1O6HX-ray2.80A/B/C2-631[»]
1O6QX-ray2.80A/B/C2-631[»]
1O6RX-ray2.70A/B/C2-631[»]
1O79X-ray2.80A/B/C2-631[»]
1SQCX-ray2.85A1-631[»]
1UMPX-ray2.13A/B/C2-631[»]
2SQCX-ray2.00A/B1-631[»]
3SQCX-ray2.80A/B/C1-631[»]
ProteinModelPortaliP33247.
SMRiP33247.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33247.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati15 – 56PFTB 1Add BLAST42
Repeati61 – 102PFTB 2Add BLAST42
Repeati241 – 282PFTB 3Add BLAST42
Repeati400 – 441PFTB 4Add BLAST42
Repeati468 – 508PFTB 5Add BLAST41
Repeati516 – 557PFTB 6Add BLAST42
Repeati574 – 622PFTB 7Add BLAST49

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 7 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105EWU. Bacteria.
COG1657. LUCA.
HOGENOMiHOG000220823.
KOiK06045.
OMAiGWAFEFH.
OrthoDBiPOG091H0DNT.

Family and domain databases

CDDicd02892. SQCY_1. 1 hit.
Gene3Di1.50.10.20. 2 hits.
InterProiIPR006400. Hopene-cyclase.
IPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01507. hopene_cyclase. 1 hit.
TIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33247-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQLVEAPA YARTLDRAVE YLLSCQKDEG YWWGPLLSNV TMEAEYVLLC
60 70 80 90 100
HILDRVDRDR MEKIRRYLLH EQREDGTWAL YPGGPPDLDT TIEAYVALKY
110 120 130 140 150
IGMSRDEEPM QKALRFIQSQ GGIESSRVFT RMWLALVGEY PWEKVPMVPP
160 170 180 190 200
EIMFLGKRMP LNIYEFGSWA RATVVALSIV MSRQPVFPLP ERARVPELYE
210 220 230 240 250
TDVPPRRRGA KGGGGWIFDA LDRALHGYQK LSVHPFRRAA EIRALDWLLE
260 270 280 290 300
RQAGDGSWGG IQPPWFYALI ALKILDMTQH PAFIKGWEGL ELYGVELDYG
310 320 330 340 350
GWMFQASISP VWDTGLAVLA LRAAGLPADH DRLVKAGEWL LDRQITVPGD
360 370 380 390 400
WAVKRPNLKP GGFAFQFDNV YYPDVDDTAV VVWALNTLRL PDERRRRDAM
410 420 430 440 450
TKGFRWIVGM QSSNGGWGAY DVDNTSDLPN HIPFCDFGEV TDPPSEDVTA
460 470 480 490 500
HVLECFGSFG YDDAWKVIRR AVEYLKREQK PDGSWFGRWG VNYLYGTGAV
510 520 530 540 550
VSALKAVGID TREPYIQKAL DWVEQHQNPD GGWGEDCRSY EDPAYAGKGA
560 570 580 590 600
STPSQTAWAL MALIAGGRAE SEAARRGVQY LVETQRPDGG WDEPYYTGTG
610 620 630
FPGDFYLGYT MYRHVFPTLA LGRYKQAIER R
Length:631
Mass (Da):71,570
Last modified:January 23, 2007 - v4
Checksum:iE389635BD6486C3A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti600 – 601GF → AS in AAA75452 (PubMed:1729216).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73834 Genomic DNA. Translation: AAA75452.1.
AB007002 Genomic DNA. Translation: BAA25185.1.
CP001727 Genomic DNA. Translation: ACV59449.1.
PIRiA43300.
RefSeqiWP_012811690.1. NC_013205.1.

Genome annotation databases

EnsemblBacteriaiACV59449; ACV59449; Aaci_2443.
KEGGiaac:Aaci_2443.
PATRICi20848382. VBIAliAci73240_2414.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73834 Genomic DNA. Translation: AAA75452.1.
AB007002 Genomic DNA. Translation: BAA25185.1.
CP001727 Genomic DNA. Translation: ACV59449.1.
PIRiA43300.
RefSeqiWP_012811690.1. NC_013205.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GSZX-ray2.80A/B/C2-631[»]
1H35X-ray2.80A/B/C2-631[»]
1H36X-ray2.80A/B/C2-631[»]
1H37X-ray2.80A/B/C2-631[»]
1H39X-ray2.80A/B/C2-631[»]
1H3AX-ray2.85A/B/C2-631[»]
1H3BX-ray2.80A/B/C2-631[»]
1H3CX-ray2.90A/B/C2-631[»]
1O6HX-ray2.80A/B/C2-631[»]
1O6QX-ray2.80A/B/C2-631[»]
1O6RX-ray2.70A/B/C2-631[»]
1O79X-ray2.80A/B/C2-631[»]
1SQCX-ray2.85A1-631[»]
1UMPX-ray2.13A/B/C2-631[»]
2SQCX-ray2.00A/B1-631[»]
3SQCX-ray2.80A/B/C1-631[»]
ProteinModelPortaliP33247.
SMRiP33247.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi521098.Aaci_2443.

Chemistry databases

BindingDBiP33247.
ChEMBLiCHEMBL3569.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACV59449; ACV59449; Aaci_2443.
KEGGiaac:Aaci_2443.
PATRICi20848382. VBIAliAci73240_2414.

Phylogenomic databases

eggNOGiENOG4105EWU. Bacteria.
COG1657. LUCA.
HOGENOMiHOG000220823.
KOiK06045.
OMAiGWAFEFH.
OrthoDBiPOG091H0DNT.

Enzyme and pathway databases

UniPathwayiUPA00337.
BioCyciMetaCyc:MONOMER-17503.
BRENDAi5.4.99.17. 243.
SABIO-RKP33247.

Miscellaneous databases

EvolutionaryTraceiP33247.
PROiP33247.

Family and domain databases

CDDicd02892. SQCY_1. 1 hit.
Gene3Di1.50.10.20. 2 hits.
InterProiIPR006400. Hopene-cyclase.
IPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01507. hopene_cyclase. 1 hit.
TIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSQHC_ALIAD
AccessioniPrimary (citable) accession number: P33247
Secondary accession number(s): C8WSG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.