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P33247 (SQHC_ALIAD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Squalene--hopene cyclase

EC=4.2.1.129
EC=5.4.99.17
Alternative name(s):
Squalene--hopanol cyclase
Gene names
Name:shc
Ordered Locus Names:Aaci_2443
OrganismAlicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / 104-1A) (Bacillus acidocaldarius) [Complete proteome] [HAMAP]
Taxonomic identifier521098 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesAlicyclobacillaceaeAlicyclobacillus

Protein attributes

Sequence length631 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the cyclization of squalene into hopene.

Catalytic activity

Squalene = hop-22(29)-ene.

Hopan-22-ol = squalene + H2O.

Pathway

Secondary metabolite biosynthesis; hopanoid biosynthesis.

Subcellular location

Cell membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the terpene cyclase/mutase family.

Contains 7 PFTB repeats.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainRepeat
   Molecular functionIsomerase
Lyase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processhopanoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlyase activity

Inferred from electronic annotation. Source: UniProtKB-KW

squalene-hopene cyclase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 631630Squalene--hopene cyclase
PRO_0000072650

Regions

Repeat15 – 5642PFTB 1
Repeat61 – 10242PFTB 2
Repeat241 – 28242PFTB 3
Repeat400 – 44142PFTB 4
Repeat468 – 50841PFTB 5
Repeat516 – 55742PFTB 6
Repeat574 – 62249PFTB 7

Experimental info

Sequence conflict600 – 6012GF → AS in AAA75452. Ref.1

Secondary structure

....................................................................................................... 631
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P33247 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: E389635BD6486C3A

FASTA63171,570
        10         20         30         40         50         60 
MAEQLVEAPA YARTLDRAVE YLLSCQKDEG YWWGPLLSNV TMEAEYVLLC HILDRVDRDR 

        70         80         90        100        110        120 
MEKIRRYLLH EQREDGTWAL YPGGPPDLDT TIEAYVALKY IGMSRDEEPM QKALRFIQSQ 

       130        140        150        160        170        180 
GGIESSRVFT RMWLALVGEY PWEKVPMVPP EIMFLGKRMP LNIYEFGSWA RATVVALSIV 

       190        200        210        220        230        240 
MSRQPVFPLP ERARVPELYE TDVPPRRRGA KGGGGWIFDA LDRALHGYQK LSVHPFRRAA 

       250        260        270        280        290        300 
EIRALDWLLE RQAGDGSWGG IQPPWFYALI ALKILDMTQH PAFIKGWEGL ELYGVELDYG 

       310        320        330        340        350        360 
GWMFQASISP VWDTGLAVLA LRAAGLPADH DRLVKAGEWL LDRQITVPGD WAVKRPNLKP 

       370        380        390        400        410        420 
GGFAFQFDNV YYPDVDDTAV VVWALNTLRL PDERRRRDAM TKGFRWIVGM QSSNGGWGAY 

       430        440        450        460        470        480 
DVDNTSDLPN HIPFCDFGEV TDPPSEDVTA HVLECFGSFG YDDAWKVIRR AVEYLKREQK 

       490        500        510        520        530        540 
PDGSWFGRWG VNYLYGTGAV VSALKAVGID TREPYIQKAL DWVEQHQNPD GGWGEDCRSY 

       550        560        570        580        590        600 
EDPAYAGKGA STPSQTAWAL MALIAGGRAE SEAARRGVQY LVETQRPDGG WDEPYYTGTG 

       610        620        630 
FPGDFYLGYT MYRHVFPTLA LGRYKQAIER R 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, expression, and sequencing of squalene-hopene cyclase, a key enzyme in triterpenoid metabolism."
Ochs D., Kaletta C., Entian K.-D., Beck-Sickinger A., Poralla K.
J. Bacteriol. 174:298-302(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Ochs D., Kaletta C., Entian K.-D., Beck-Sickinger A., Poralla K.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Overexpression of squalene-hopene cyclase by the pET vector in Escherichia coli and first identification of tryptophan and aspartic acid residues inside the QW motif as active sites."
Sato T., Kanai Y., Hoshino T.
Biosci. Biotechnol. Biochem. 62:407-411(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The complete chromosome of Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Ovchinnikova G., Chertkov O., Sims D., Brettin T., Detter J.C., Han C. expand/collapse author list , Larimer F., Land M., Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Pukall R., Klenk H.-P., Eisen J.A.
Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27009 / DSM 446 / 104-1A.
[5]"Properties of purified squalene-hopene cyclase from Bacillus acidocaldarius."
Ochs D., Tappe C.H., Gaertner P., Kellner R., Poralla K.
Eur. J. Biochem. 194:75-80(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-25, CHARACTERIZATION.
[6]"Structure and function of a squalene cyclase."
Wendt K.U., Poralla K., Schulz G.E.
Science 277:1811-1815(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
[7]"The structure of the membrane protein squalene-hopene cyclase at 2.0-A resolution."
Wendt K.U., Lenhart A., Schulz G.E.
J. Mol. Biol. 286:175-187(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M73834 Genomic DNA. Translation: AAA75452.1.
AB007002 Genomic DNA. Translation: BAA25185.1.
CP001727 Genomic DNA. Translation: ACV59449.1.
PIRA43300.
RefSeqYP_003185838.1. NC_013205.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GSZX-ray2.80A/B/C2-631[»]
1H35X-ray2.80A/B/C2-631[»]
1H36X-ray2.80A/B/C2-631[»]
1H37X-ray2.80A/B/C2-631[»]
1H39X-ray2.80A/B/C2-631[»]
1H3AX-ray2.85A/B/C2-631[»]
1H3BX-ray2.80A/B/C2-631[»]
1H3CX-ray2.90A/B/C2-631[»]
1O6HX-ray2.80A/B/C2-631[»]
1O6QX-ray2.80A/B/C2-631[»]
1O6RX-ray2.70A/B/C2-631[»]
1O79X-ray2.80A/B/C2-631[»]
1SQCX-ray2.85A1-631[»]
1UMPX-ray2.13A/B/C2-631[»]
2SQCX-ray2.00A/B1-631[»]
3SQCX-ray2.80A/B/C1-631[»]
ProteinModelPortalP33247.
SMRP33247. Positions 10-628.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING521098.Aaci_2443.

Chemistry

BindingDBP33247.
ChEMBLCHEMBL3569.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACV59449; ACV59449; Aaci_2443.
GeneID8425972.
KEGGaac:Aaci_2443.
PATRIC20848382. VBIAliAci73240_2414.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1657.
HOGENOMHOG000220823.
KOK06045.
OMAWAFEYAN.
OrthoDBEOG6K6V4Q.

Enzyme and pathway databases

BioCycAACI521098:GCIO-2514-MONOMER.
MetaCyc:MONOMER-17503.
SABIO-RKP33247.
UniPathwayUPA00337.

Family and domain databases

Gene3D1.50.10.20. 2 hits.
InterProIPR006400. Hopene-cyclase.
IPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamPF00432. Prenyltrans. 3 hits.
[Graphical view]
SUPFAMSSF48239. SSF48239. 2 hits.
TIGRFAMsTIGR01507. hopene_cyclase. 1 hit.
TIGR01787. squalene_cyclas. 1 hit.
PROSITEPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP33247.

Entry information

Entry nameSQHC_ALIAD
AccessionPrimary (citable) accession number: P33247
Secondary accession number(s): C8WSG4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 112 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways