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Protein

Nuclear hormone receptor FTZ-F1

Gene

ftz-f1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a cofactor to fushi tarazu (ftz). Facilitates the binding of ftz to DNA. Binds the sequence element 5'-YCYYGGYCR-3' in the zebra element of ftz. Probably also functions as a receptor for a yet unknown ligand.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi505 – 580Nuclear receptorPROSITE-ProRule annotationAdd BLAST76
Zinc fingeri508 – 528NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri544 – 568NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • cell death Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • imaginal disc-derived leg morphogenesis Source: FlyBase
  • instar larval or pupal development Source: FlyBase
  • juvenile hormone mediated signaling pathway Source: FlyBase
  • lipid homeostasis Source: FlyBase
  • metamorphosis Source: FlyBase
  • mushroom body development Source: FlyBase
  • neuron remodeling Source: FlyBase
  • olfactory behavior Source: FlyBase
  • periodic partitioning Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • pupariation Source: FlyBase
  • pupation Source: FlyBase
  • regulation of development, heterochronic Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • response to ecdysone Source: FlyBase
  • response to hormone Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-383280. Nuclear Receptor transcription pathway.
SignaLinkiP33244.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear hormone receptor FTZ-F1
Alternative name(s):
FTZ-F1 alpha
Nuclear receptor subfamily 5 group A member 3
Gene namesi
Name:ftz-f1
Synonyms:NR5A3
ORF Names:CG4059
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0001078. ftz-f1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • nucleus Source: FlyBase
  • RNA polymerase II transcription factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Ftz-like pair-rule cuticular defects.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi563C → S: 100-fold less binding. 1 Publication1
Mutagenesisi579R → Q: 100-fold less binding. 1 Publication1
Mutagenesisi582R → Q: 100-fold less binding. 1 Publication1
Mutagenesisi583M → I: 10-fold less binding. 1 Publication1
Mutagenesisi584R → Q: 10-fold less binding. 1 Publication1
Mutagenesisi585 – 586GG → AA: No binding. 1 Publication2
Mutagenesisi587R → Q: 10-fold less reduced binding. 1 Publication1
Mutagenesisi589K → Q: No effect on binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000537391 – 1027Nuclear hormone receptor FTZ-F1Add BLAST1027

Proteomic databases

PaxDbiP33244.
PRIDEiP33244.

Expressioni

Tissue specificityi

Expression in the parasegmental primordia of the embryonic blastoderm.1 Publication

Developmental stagei

First appears in blastoderm embryos. It is absent in subsequent embryo stages, and then reappears in late embryogenesis to be found in larvae, pupae and adults.1 Publication

Gene expression databases

BgeeiFBgn0001078.
ExpressionAtlasiP33244. baseline.
GenevisibleiP33244. DM.

Interactioni

Subunit structurei

Monomer; forms a complex with ftz.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ftzP028353EBI-160447,EBI-125786

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi65326. 28 interactors.
IntActiP33244. 3 interactors.
MINTiMINT-156933.
STRINGi7227.FBpp0074853.

Structurei

Secondary structure

11027
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi790 – 792Combined sources3
Helixi795 – 802Combined sources8
Helixi806 – 821Combined sources16
Helixi829 – 849Combined sources21
Helixi854 – 856Combined sources3
Helixi859 – 884Combined sources26
Beta strandi889 – 892Combined sources4
Beta strandi894 – 896Combined sources3
Beta strandi898 – 900Combined sources3
Helixi901 – 905Combined sources5
Turni906 – 908Combined sources3
Helixi910 – 912Combined sources3
Helixi913 – 925Combined sources13
Helixi930 – 941Combined sources12
Helixi952 – 973Combined sources22
Helixi980 – 1007Combined sources28
Beta strandi1013 – 1015Combined sources3
Helixi1016 – 1022Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XHSX-ray2.80A790-1027[»]
ProteinModelPortaliP33244.
SMRiP33244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni834 – 876Ligand-bindingSequence analysisAdd BLAST43

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 45Gln-rich (OPA repeat)Add BLAST17
Compositional biasi155 – 169Poly-AsnAdd BLAST15
Compositional biasi359 – 365Poly-Gln (OPA repeat)7
Compositional biasi412 – 495Gly-richAdd BLAST84
Compositional biasi686 – 738Gly-richAdd BLAST53

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri508 – 528NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri544 – 568NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4218. Eukaryota.
ENOG410YWC2. LUCA.
GeneTreeiENSGT00850000132285.
InParanoidiP33244.
KOiK08705.
OrthoDBiEOG091G06RK.
PhylomeDBiP33244.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR016355. NR5_fam.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR24086. PTHR24086. 1 hit.
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P33244-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDTFNVPMLA ESSNTNYATE ATSNHHHLQH QHQQQHSHQQ QQQQQLLMPH
60 70 80 90 100
HHKDQMLAAG SSPMLPFYSH LQLQQKDATA TIGPAAAAAA VEAATTSANA
110 120 130 140 150
DNFSSLQTID ASQLDGGISL SGLCDRFFVA SPNPHSNSNM TLMGTATAAT
160 170 180 190 200
TTTTNNNNNN NTNNNNNNNV EAKTVRPSNG NSVIIESVTM PSFANILFPT
210 220 230 240 250
HRSANECIDP ALLQKNPQNP NGNNSSIIVP PVEYHQLKPL EVNSSTSVST
260 270 280 290 300
SNFLSSTTAQ LLDFEVQVGK DDGHISTTTT TGPGSGSASG SGSGSGSGSG
310 320 330 340 350
SIARTIGTAT PTTTTSMSNT ANPTRSSLHS IEELAASSCA PRAASPNSNH
360 370 380 390 400
TSSASTTPQQ QQQQQHHMQS GNHSGSNLSS DDESMSEDEF GLEIDDNGGY
410 420 430 440 450
QDTTSSHSQQ SGGGGGGGGG NLLNGSSGGS SAGGGYMLLP QAASSSGNNG
460 470 480 490 500
NPNAGHMSSG SVGNGSGGAG NGGAGGNSGP GNPMGGTSAT PGHGGEVIDF
510 520 530 540 550
KHLFEELCPV CGDKVSGYHY GLLTCESCKG FFKRTVQNKK VYTCVAERSC
560 570 580 590 600
HIDKTQRKRC PYCRFQKCLE VGMKLEAVRA DRMRGGRNKF GPMYKRDRAR
610 620 630 640 650
KLQVMRQRQL ALQALRNSMG PDIKPTPISP GYQQAYPNMN IKQEIQIPQV
660 670 680 690 700
SSLTQSPDSS PSPIAIALGQ VNASTGGVIA TPMNAGTGGS GGGGLNGPSS
710 720 730 740 750
VGNGNSSNGS SNGNNNSSTG NGTSGGGGGN NAGGGGGGTN SNDGLHRNGG
760 770 780 790 800
NGNSSCHEAG IGSLQNTADS KLCFDSGTHP SSTADALIEP LRVSPMIREF
810 820 830 840 850
VQSIDDREWQ TQLFALLQKQ TYNQVEVDLF ELMCKVLDQN LFSQVDWARN
860 870 880 890 900
TVFFKDLKVD DQMKLLQHSW SDMLVLDHLH HRIHNGLPDE TQLNNGQVFN
910 920 930 940 950
LMSLGLLGVP QLGDYFNELQ NKLQDLKFDM GDYVCMKFLI LLNPSVRGIV
960 970 980 990 1000
NRKTVSEGHD NVQAALLDYT LTCYPSVNDK FRGLVNILPE IHAMAVRGED
1010 1020
HLYTKHCAGS APTQTLLMEM LHAKRKG
Length:1,027
Mass (Da):108,418
Last modified:March 15, 2004 - v2
Checksum:i6A2240934830290D
GO
Isoform A (identifier: P33244-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-174: MDTFNVPMLA...NNNNNVEAKT → MLLEMDQQQA...NFDSQYIFPT
     175-398: Missing.

Show »
Length:803
Mass (Da):85,900
Checksum:iF20BE9D5EC07D015
GO

Sequence cautioni

The sequence AAA28542 differs from that shown. Reason: Frameshift at positions 1003, 1004 and 1005.Curated
The sequence AAA28915 differs from that shown. Reason: Frameshift at positions 1003, 1004 and 1005.Curated
The sequence AAK93269 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45Q → QQQ AA sequence (PubMed:1709303).Curated1
Sequence conflicti304R → S in AAA28542 (PubMed:1709303).Curated1
Sequence conflicti752 – 753GN → DS in AAA28542 (PubMed:1709303).Curated2
Sequence conflicti752 – 753GN → DS in AAA28915 (PubMed:8096644).Curated2
Sequence conflicti832L → LL in AAA28542 (PubMed:1709303).Curated1
Sequence conflicti832L → LL in AAA28915 (PubMed:8096644).Curated1
Sequence conflicti912L → P in AAA28542 (PubMed:1709303).Curated1
Sequence conflicti912L → P in AAA28915 (PubMed:8096644).Curated1
Isoform A (identifier: P33244-2)
Sequence conflicti67 – 68NN → N in AAA28915 (PubMed:8096644).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0129171 – 174MDTFN…VEAKT → MLLEMDQQQATVQFISSLNI SPFSMQLEQQQQPSSPALAA GGNSSNNAASGSNNNSASGN NTSSSSNNNNNNNNDNDAHV LTKFEHEYNAYTLQLAGGGG SGSGNQQHHSNHSNHGNHHQ QQQQQQQQQQQHQQQQQEHY QQQQQQNIANNANQFNSSSY SYIYNFDSQYIFPT in isoform A. 1 PublicationAdd BLAST174
Alternative sequenceiVSP_012918175 – 398Missing in isoform A. 1 PublicationAdd BLAST224

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63711 mRNA. Translation: AAA28542.1. Frameshift.
M98397 mRNA. Translation: AAA28915.1. Frameshift.
AE014296 Genomic DNA. Translation: AAF49231.2.
AE014296 Genomic DNA. Translation: AAN11667.1.
BT058016 mRNA. Translation: ACM16732.1.
AY051845 mRNA. Translation: AAK93269.1. Different initiation.
PIRiA47303.
T13733.
RefSeqiNP_001246824.1. NM_001259895.2. [P33244-2]
NP_524143.2. NM_079419.3. [P33244-1]
NP_730359.1. NM_168775.2. [P33244-2]
UniGeneiDm.995.

Genome annotation databases

EnsemblMetazoaiFBtr0075086; FBpp0074853; FBgn0001078. [P33244-1]
GeneIDi40045.
KEGGidme:Dmel_CG4059.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63711 mRNA. Translation: AAA28542.1. Frameshift.
M98397 mRNA. Translation: AAA28915.1. Frameshift.
AE014296 Genomic DNA. Translation: AAF49231.2.
AE014296 Genomic DNA. Translation: AAN11667.1.
BT058016 mRNA. Translation: ACM16732.1.
AY051845 mRNA. Translation: AAK93269.1. Different initiation.
PIRiA47303.
T13733.
RefSeqiNP_001246824.1. NM_001259895.2. [P33244-2]
NP_524143.2. NM_079419.3. [P33244-1]
NP_730359.1. NM_168775.2. [P33244-2]
UniGeneiDm.995.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XHSX-ray2.80A790-1027[»]
ProteinModelPortaliP33244.
SMRiP33244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65326. 28 interactors.
IntActiP33244. 3 interactors.
MINTiMINT-156933.
STRINGi7227.FBpp0074853.

Proteomic databases

PaxDbiP33244.
PRIDEiP33244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075086; FBpp0074853; FBgn0001078. [P33244-1]
GeneIDi40045.
KEGGidme:Dmel_CG4059.

Organism-specific databases

CTDi40045.
FlyBaseiFBgn0001078. ftz-f1.

Phylogenomic databases

eggNOGiKOG4218. Eukaryota.
ENOG410YWC2. LUCA.
GeneTreeiENSGT00850000132285.
InParanoidiP33244.
KOiK08705.
OrthoDBiEOG091G06RK.
PhylomeDBiP33244.

Enzyme and pathway databases

ReactomeiR-DME-383280. Nuclear Receptor transcription pathway.
SignaLinkiP33244.

Miscellaneous databases

ChiTaRSiftz-f1. fly.
GenomeRNAii40045.
PROiP33244.

Gene expression databases

BgeeiFBgn0001078.
ExpressionAtlasiP33244. baseline.
GenevisibleiP33244. DM.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR016355. NR5_fam.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR24086. PTHR24086. 1 hit.
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFTZF1_DROME
AccessioniPrimary (citable) accession number: P33244
Secondary accession number(s): B9EQU1
, Q24538, Q8IQT5, Q960U2, Q9VVS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 15, 2004
Last modified: November 30, 2016
This is version 166 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.