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Protein

Steroidogenic factor 1

Gene

Nr5a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity (By similarity). Transcription repressor of the Moloney leukemia virus long terminal repeat in undifferentiated murine embryonal carcinoma cells. Binds phosphatidylcholine and phospholipids with a phosphatidylinositol (PI) headgroup, in particular phosphatidyl(3,4)bisphosphate, phosphatidyl(3,5)bisphosphate and phosphatidyl(3,4,5)triphosphate. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei342Lipid headgroup; via amide nitrogen1 Publication1
Binding sitei437Lipid headgroup1 Publication1
Binding sitei441Lipid headgroup1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi10 – 85Nuclear receptorPROSITE-ProRule annotationAdd BLAST76
Zinc fingeri13 – 33NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri49 – 73NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • adrenal gland development Source: MGI
  • cell differentiation Source: MGI
  • hormone-mediated signaling pathway Source: GO_Central
  • hormone metabolic process Source: MGI
  • luteinization Source: MGI
  • maintenance of protein location in nucleus Source: MGI
  • male gonad development Source: MGI
  • multicellular organism aging Source: MGI
  • negative regulation of female gonad development Source: UniProtKB
  • positive regulation of male gonad development Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • regulation of transcription, DNA-templated Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • reproductive process Source: MGI
  • tissue development Source: MGI
  • transcription from RNA polymerase II promoter Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Lipid-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.

Chemistry databases

SwissLipidsiSLP:000001543.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroidogenic factor 1
Short name:
SF-1
Short name:
STF-1
Alternative name(s):
Adrenal 4-binding protein
Embryonal LTR-binding protein
Short name:
ELP
Embryonal long terminal repeat-binding protein
Fushi tarazu factor homolog 1
Nuclear receptor subfamily 5 group A member 1
Steroid hormone receptor Ad4BP
Steroid hydroxylase positive regulator
Gene namesi
Name:Nr5a1
Synonyms:Ftzf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1346833. Nr5a1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: HGNC
  • RNA polymerase II transcription factor complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi270A → W: Reduced lipid binding and transactivation. Abolishes ligand binding and reduces transactivation; when associated with F-345. 2 Publications1
Mutagenesisi314R → M: Strongly reduced transactivation. 1 Publication1
Mutagenesisi345L → F: Abolishes ligand binding and reduces transactivation; when associated with W-270. 2 Publications1
Mutagenesisi345L → W: Strongly reduced transactivation. 2 Publications1
Mutagenesisi348L → W: Strongly reduced transactivation. 1 Publication1
Mutagenesisi349V → W: Strongly reduced transactivation. 1 Publication1
Mutagenesisi352A → W: Strongly reduced transactivation. 1 Publication1
Mutagenesisi434A → W: Strongly reduced transactivation. 1 Publication1
Mutagenesisi440H → D: Strongly reduced transactivation; when associated with D-442. 1
Mutagenesisi441K → E: Strongly reduced transactivation. 1 Publication1
Mutagenesisi442H → D: Strongly reduced transactivation; when associated with D-440. 1

Chemistry databases

ChEMBLiCHEMBL1764943.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000537321 – 462Steroidogenic factor 1Add BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki119Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei203Phosphoserine; by CDK7By similarity1

Post-translational modificationi

Acetylation stimulates the transcriptional activity.By similarity
Sumoylation reduces CDK7-mediated phosphorylation on Ser-203.By similarity
Phosphorylated on Ser-203 by CDK7. This phosphorylation promotes transcriptional activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP33242.
PRIDEiP33242.

PTM databases

iPTMnetiP33242.
PhosphoSitePlusiP33242.

Expressioni

Developmental stagei

Expressed during early embryonic development. Expressed only in undifferentiated embryonal carcinoma cells.

Gene expression databases

BgeeiENSMUSG00000026751.
CleanExiMM_NR5A1.
GenevisibleiP33242. MM.

Interactioni

Subunit structurei

Binds DNA as a monomer (By similarity). Part of a complex consisting of SFPQ, NONO and NR5A1. Interacts with DGKQ and CDK7 (By similarity). Interacts with NR0B2, NCOA2 and PPARGC1A. Binds to and activated by HIPK3.By similarity5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204975. 18 interactors.
IntActiP33242. 1 interactor.
STRINGi10090.ENSMUSP00000028084.

Structurei

Secondary structure

1462
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 16Combined sources3
Beta strandi22 – 24Combined sources3
Beta strandi27 – 29Combined sources3
Helixi31 – 41Combined sources11
Turni42 – 44Combined sources3
Helixi61 – 64Combined sources4
Helixi66 – 76Combined sources11
Helixi80 – 82Combined sources3
Turni94 – 97Combined sources4
Helixi98 – 106Combined sources9
Beta strandi220 – 222Combined sources3
Helixi226 – 234Combined sources9
Helixi238 – 249Combined sources12
Beta strandi252 – 254Combined sources3
Turni255 – 257Combined sources3
Helixi263 – 283Combined sources21
Helixi287 – 289Combined sources3
Helixi292 – 318Combined sources27
Beta strandi323 – 325Combined sources3
Beta strandi331 – 333Combined sources3
Helixi334 – 340Combined sources7
Helixi343 – 361Combined sources19
Helixi366 – 377Combined sources12
Helixi382 – 384Combined sources3
Helixi388 – 409Combined sources22
Turni411 – 413Combined sources3
Helixi416 – 443Combined sources28
Helixi452 – 458Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YMTX-ray1.20A219-462[»]
1YP0X-ray1.50A223-461[»]
2FF0NMR-A10-111[»]
3F7DX-ray2.20A219-462[»]
ProteinModelPortaliP33242.
SMRiP33242.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33242.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni263 – 348Ligand-bindingAdd BLAST86

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri13 – 33NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri49 – 73NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4218. Eukaryota.
ENOG410YWC2. LUCA.
GeneTreeiENSGT00850000132285.
HOVERGENiHBG106677.
InParanoidiP33242.
KOiK08560.
OMAiLQEPAKG.
OrthoDBiEOG091G06RK.
TreeFamiTF350737.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR016355. NR5_fam.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR24086. PTHR24086. 1 hit.
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PIRSFiPIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P33242-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDYSYDEDLD ELCPVCGDKV SGYHYGLLTC ESCKGFFKRT VQNNKHYTCT
60 70 80 90 100
ESQSCKIDKT QRKRCPFCRF QKCLTVGMRL EAVRADRMRG GRNKFGPMYK
110 120 130 140 150
RDRALKQQKK AQIRANGFKL ETGPPMGVPP PPPPPPDYML PPSLHAPEPK
160 170 180 190 200
ALVSGPPSGP LGDFGAPSLP MAVPGPHGPL AGYLYPAFSN RTIKSEYPEP
210 220 230 240 250
YASPPQQPGP PYSYPEPFSG GPNVPELILQ LLQLEPEEDQ VRARIVGCLQ
260 270 280 290 300
EPAKSRSDQP APFSLLCRMA DQTFISIVDW ARRCMVFKEL EVADQMTLLQ
310 320 330 340 350
NCWSELLVLD HIYRQVQYGK EDSILLVTGQ EVELSTVAVQ AGSLLHSLVL
360 370 380 390 400
RAQELVLQLH ALQLDRQEFV CLKFLILFSL DVKFLNNHSL VKDAQEKANA
410 420 430 440 450
ALLDYTLCHY PHCGDKFQQL LLCLVEVRAL SMQAKEYLYH KHLGNEMPRN
460
NLLIEMLQAK QT
Length:462
Mass (Da):52,077
Last modified:July 27, 2011 - v3
Checksum:i67045B563403CF5A
GO
Isoform 2 (identifier: P33242-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     333-462: ELSTVAVQAG...LIEMLQAKQT → TELVKPLVLH...GMVSGSSYRR

Show »
Length:388
Mass (Da):43,387
Checksum:iE9A38FD8427F5888
GO

Sequence cautioni

The sequence BAA01441 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164F → I in BAA01441 (PubMed:1545809).Curated1
Sequence conflicti256R → G in BAA01441 (PubMed:1545809).Curated1
Sequence conflicti328T → S in BAA01441 (PubMed:1545809).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti172A → S in strain: C3H/HeJ and DBA/2J; does not change transcriptional activity. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003715333 – 462ELSTV…QAKQT → TELVKPLVLHNPRPLRADSG HPKFQIQGHALARLLCVLGP FEEPQCGMVSGSSYRR in isoform 2. 1 PublicationAdd BLAST130

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10584 mRNA. Translation: BAA01441.1. Different initiation.
S65878
, S65875, S65919, S65876, S65877 Genomic DNA. Translation: AAB28338.1.
S65876, S65875, S65919 Genomic DNA. Translation: AAB28339.1.
AF511594 mRNA. Translation: AAM43952.1.
AK134378 mRNA. Translation: BAE22121.1.
AK144376 mRNA. Translation: BAE25855.1.
AL844842 Genomic DNA. Translation: CAM21930.1.
BC110476 mRNA. Translation: AAI10477.1.
BC110477 mRNA. Translation: AAI10478.1.
CCDSiCCDS16011.1. [P33242-1]
PIRiA40716.
A42128.
RefSeqiNP_001303616.1. NM_001316687.1. [P33242-1]
NP_620639.1. NM_139051.3. [P33242-1]
UniGeneiMm.31387.

Genome annotation databases

EnsembliENSMUST00000028084; ENSMUSP00000028084; ENSMUSG00000026751. [P33242-1]
ENSMUST00000112883; ENSMUSP00000108504; ENSMUSG00000026751. [P33242-1]
GeneIDi26423.
KEGGimmu:26423.
UCSCiuc008jnr.1. mouse. [P33242-1]
uc008jnt.1. mouse. [P33242-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10584 mRNA. Translation: BAA01441.1. Different initiation.
S65878
, S65875, S65919, S65876, S65877 Genomic DNA. Translation: AAB28338.1.
S65876, S65875, S65919 Genomic DNA. Translation: AAB28339.1.
AF511594 mRNA. Translation: AAM43952.1.
AK134378 mRNA. Translation: BAE22121.1.
AK144376 mRNA. Translation: BAE25855.1.
AL844842 Genomic DNA. Translation: CAM21930.1.
BC110476 mRNA. Translation: AAI10477.1.
BC110477 mRNA. Translation: AAI10478.1.
CCDSiCCDS16011.1. [P33242-1]
PIRiA40716.
A42128.
RefSeqiNP_001303616.1. NM_001316687.1. [P33242-1]
NP_620639.1. NM_139051.3. [P33242-1]
UniGeneiMm.31387.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YMTX-ray1.20A219-462[»]
1YP0X-ray1.50A223-461[»]
2FF0NMR-A10-111[»]
3F7DX-ray2.20A219-462[»]
ProteinModelPortaliP33242.
SMRiP33242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204975. 18 interactors.
IntActiP33242. 1 interactor.
STRINGi10090.ENSMUSP00000028084.

Chemistry databases

ChEMBLiCHEMBL1764943.
SwissLipidsiSLP:000001543.

PTM databases

iPTMnetiP33242.
PhosphoSitePlusiP33242.

Proteomic databases

PaxDbiP33242.
PRIDEiP33242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028084; ENSMUSP00000028084; ENSMUSG00000026751. [P33242-1]
ENSMUST00000112883; ENSMUSP00000108504; ENSMUSG00000026751. [P33242-1]
GeneIDi26423.
KEGGimmu:26423.
UCSCiuc008jnr.1. mouse. [P33242-1]
uc008jnt.1. mouse. [P33242-2]

Organism-specific databases

CTDi2516.
MGIiMGI:1346833. Nr5a1.

Phylogenomic databases

eggNOGiKOG4218. Eukaryota.
ENOG410YWC2. LUCA.
GeneTreeiENSGT00850000132285.
HOVERGENiHBG106677.
InParanoidiP33242.
KOiK08560.
OMAiLQEPAKG.
OrthoDBiEOG091G06RK.
TreeFamiTF350737.

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.

Miscellaneous databases

EvolutionaryTraceiP33242.
PROiP33242.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026751.
CleanExiMM_NR5A1.
GenevisibleiP33242. MM.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR016355. NR5_fam.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR24086. PTHR24086. 1 hit.
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PIRSFiPIRSF002530. Nuc_orph_FTZ-F1. 1 hit.
PRINTSiPR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTF1_MOUSE
AccessioniPrimary (citable) accession number: P33242
Secondary accession number(s): Q812G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 156 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.