P33241 (LSP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lymphocyte-specific protein 1

Alternative name(s):
47 kDa actin-binding protein
52 kDa phosphoprotein
Short name=pp52
Lymphocyte-specific antigen WP34

Gene names

Name:	LSP1
Synonyms:	WP34

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in mediating neutrophil activation and chemotaxis By similarity.
Subunit structure	Binds actin.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue specificity	Activated T-lymphocytes.
Post-translational modification	Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis By similarity. Ref.9 Ref.10

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular component movement Traceable author statement Ref.3. Source: ProtInc cellular defense response Traceable author statement Ref.2. Source: ProtInc
Cellular_component	actin cytoskeleton Traceable author statement Ref.3. Source: ProtInc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	actin binding Traceable author statement Ref.3. Source: ProtInc signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P33241-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P33241-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: P33241-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MAEASSDPGAEEREELLG → MAPIWSPPGR...SGPSTNCSPR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Lymphocyte-specific protein 1		PRO_0000084503
Amino acid modifications
	Modified residue	85	1	Phosphoserine By similarity
	Modified residue	175	1	Phosphothreonine By similarity
	Modified residue	177	1	Phosphoserine By similarity
	Modified residue	189	1	Phosphoserine Ref.12
	Modified residue	252	1	Phosphoserine; by MAPKAPK2 Ref.9 Ref.10 Ref.11
	Modified residue	327	1	N6-acetyllysine Ref.13
Natural variations
	Alternative sequence	1 – 62	62	Missing in isoform 2.		VSP_045655
	Alternative sequence	1 – 18	18	MAEAS…EELLG → MAPIWSPPGRVSGCHLSSGP APGSAVGPWLGTPHPSLPLP LAPHKPPPPGLPGSAGQTSL PAQRECVFPGDAAVHQELCG LGFEECLGSIPQAHQCYLTN GPKRRKCSPRRRGRAPAWLC GGSPPCHQGLGHEHPSSGPS TNCSPR in isoform 3.		VSP_045983
	Natural variant	100	1	A → T. Ref.5 Ref.6 Ref.8 Corresponds to variant rs621679 [ dbSNP | Ensembl ].		VAR_011867
	Natural variant	108	1	Q → L. Corresponds to variant rs11545725 [ dbSNP | Ensembl ].		VAR_061680
	Natural variant	229	1	Q → K. Corresponds to variant rs1803928 [ dbSNP | Ensembl ].		VAR_011868
Experimental info
	Sequence conflict	15	1	Missing in AAB29545. Ref.3
	Sequence conflict	24	1	S → T in CAA38971. Ref.2
	Sequence conflict	100 – 101	2	Missing in AAB29545. Ref.3
	Sequence conflict	148	1	E → D in BAG52478. Ref.5
Isoform 3:
	Sequence conflict	31	1	G → Q in BAG52478. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: F2A18533500611D9	FASTA	339	37,192
	10 20 30 40 50 60 MAEASSDPGA EEREELLGPT AQWSVEDEEE AVHEQCQHER DRQLQAQDEE GGGHVPERPK 70 80 90 100 110 120 QEMLLSLKPS EAPELDEDEG FGDWSQRPEQ RQQHEGAQGA LDSGEPPQCR SPEGEQEDRP 130 140 150 160 170 180 GLHAYEKEDS DEVHLEELSL SKEGPGPEDT VQDNLGAAGA EEEQEEHQKC QQPRTPSPLV 190 200 210 220 230 240 LEGTIEQSSP PLSPTTKLID RTESLNRSIE KSNSVKKSQP DLPISKIDQW LEQYTQAIET 250 260 270 280 290 300 AGRTPKLARQ ASIELPSMAV ASTKSRWETG EVQAQSAAKT PSCKDIVAGD MSKKSLWEQK 310 320 330 GGSKTSSTIK STPSGKRYKF VATGHGKYEK VLVEGGPAP
	Isoform 2 [UniParc]. Checksum: DA869746FA7B7C6D	FASTA	277	30,213
	10 20 30 40 50 60 MLLSLKPSEA PELDEDEGFG DWSQRPEQRQ QHEGAQGALD SGEPPQCRSP EGEQEDRPGL 70 80 90 100 110 120 HAYEKEDSDE VHLEELSLSK EGPGPEDTVQ DNLGAAGAEE EQEEHQKCQQ PRTPSPLVLE 130 140 150 160 170 180 GTIEQSSPPL SPTTKLIDRT ESLNRSIEKS NSVKKSQPDL PISKIDQWLE QYTQAIETAG 190 200 210 220 230 240 RTPKLARQAS IELPSMAVAS TKSRWETGEV QAQSAAKTPS CKDIVAGDMS KKSLWEQKGG 250 260 270 SKTSSTIKST PSGKRYKFVA TGHGKYEKVL VEGGPAP
	Isoform 3 [UniParc]. Checksum: A3F09D8EECD570D6	FASTA	467	50,389
	10 20 30 40 50 60 MAPIWSPPGR VSGCHLSSGP APGSAVGPWL GTPHPSLPLP LAPHKPPPPG LPGSAGQTSL 70 80 90 100 110 120 PAQRECVFPG DAAVHQELCG LGFEECLGSI PQAHQCYLTN GPKRRKCSPR RRGRAPAWLC 130 140 150 160 170 180 GGSPPCHQGL GHEHPSSGPS TNCSPRPTAQ WSVEDEEEAV HEQCQHERDR QLQAQDEEGG 190 200 210 220 230 240 GHVPERPKQE MLLSLKPSEA PELDEDEGFG DWSQRPEQRQ QHEGAQGALD SGEPPQCRSP 250 260 270 280 290 300 EGEQEDRPGL HAYEKEDSDE VHLEELSLSK EGPGPEDTVQ DNLGAAGAEE EQEEHQKCQQ 310 320 330 340 350 360 PRTPSPLVLE GTIEQSSPPL SPTTKLIDRT ESLNRSIEKS NSVKKSQPDL PISKIDQWLE 370 380 390 400 410 420 QYTQAIETAG RTPKLARQAS IELPSMAVAS TKSRWETGEV QAQSAAKTPS CKDIVAGDMS 430 440 450 460 KKSLWEQKGG SKTSSTIKST PSGKRYKFVA TGHGKYEKVL VEGGPAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human and mouse LSP1 genes code for highly conserved phosphoproteins." Jongstra-Bilen J., Young A.J., Chong R., Jongstra J. J. Immunol. 144:1104-1110(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Molecular cloning and characterization of WP34, a phosphorylated human lymphocyte differentiation and activation antigen." Kadiyala R.K., McIntyre B.W., Krensky A.M. Eur. J. Immunol. 20:2417-2423(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Lymphocyte.
[3]	"The 47-kD protein increased in neutrophil actin dysfunction with 47- and 89-kD protein abnormalities is lymphocyte-specific protein." Howard T., Li Y., Torres M., Guerrero A., Coates T. Blood 83:231-241(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANT THR-100. Tissue: Esophagus and Tongue.
[6]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-100. Tissue: Dermoid cancer.
[7]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-100. Tissue: Lymph.
[9]	"MAPKAPK2-mediated LSP1 phosphorylation and FMLP-induced neutrophil polarization." Wu Y., Zhan L., Ai Y., Hannigan M., Gaestel M., Huang C.-K., Madri J.A. Biochem. Biophys. Res. Commun. 358:170-175(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-252 BY MAPKAPK2.
[10]	Carrascal M., Abian J. Submitted (JUN-2007) to UniProtKB Cited for: PHOSPHORYLATION AT SER-252, MASS SPECTROMETRY.
[11]	"Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment." Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J. J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252, MASS SPECTROMETRY. Tissue: T-cell.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-327, MASS SPECTROMETRY.
[14]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M33552 mRNA. Translation: AAA59534.1.
X55188 mRNA. Translation: CAA38971.1.
S67783 mRNA. Translation: AAB29545.1.
CR541728 mRNA. Translation: CAG46529.1.
CR541754 mRNA. Translation: CAG46554.1.
AK056576 mRNA. Translation: BAG51753.1.
AK092071 mRNA. Translation: BAG52478.1.
AK222733 mRNA. Translation: BAD96453.1.
AC051649 Genomic DNA. No translation available.
BC001785 mRNA. Translation: AAH01785.1.

IPI

IPI00013260.
IPI00554652.
IPI00789393.

PIR

A43542.

RefSeq

NP_001013271.1. NM_001013253.1.
NP_001013272.1. NM_001013254.1.
NP_001013273.1. NM_001013255.1.
NP_001229861.1. NM_001242932.1.
NP_002330.1. NM_002339.2.

UniGene

Hs.56729.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BH8	X-ray	1.65	C	249-258	[»]

ProteinModelPortal

P33241.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000308383.

PTM databases

PhosphoSite

P33241.

Polymorphism databases

DMDM

462553.

2D gel databases

SWISS-2DPAGE

P33241.

Proteomic databases

PaxDb

P33241.

PeptideAtlas

P33241.

PRIDE

P33241.

Protocols and materials databases

DNASU

4046.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000311604; ENSP00000308383; ENSG00000130592.
ENST00000381775; ENSP00000371194; ENSG00000130592.
ENST00000405957; ENSP00000383932; ENSG00000130592.
ENST00000406638; ENSP00000384022; ENSG00000130592.

GeneID

4046.

KEGG

hsa:4046.

UCSC

uc001lui.3. human.
uc001luj.3. human.

Organism-specific databases

CTD

4046.

GeneCards

GC11P001874.

HGNC

HGNC:6707. LSP1.

HPA

CAB017781.
HPA019693.

MIM

153432. gene.

neXtProt

NX_P33241.

PharmGKB

PA30472.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG302084.

HOGENOM

HOG000231050.

HOVERGEN

HBG001610.

KO

K14957.

OrthoDB

EOG4NKBW0.

PhylomeDB

P33241.

Enzyme and pathway databases

Pathway_Interaction_DB

p38_mk2pathway. p38 signaling mediated by MAPKAP kinases.

SignaLink

P33241.

Gene expression databases

ArrayExpress

P33241.

Bgee

P33241.

CleanEx

HS_LSP1.

Genevestigator

P33241.

GermOnline

ENSG00000130592. Homo sapiens.

Family and domain databases

InterPro

IPR006018. Caldesmon_LSP.
IPR002211. Lymphspecific.
[Graphical view]

PANTHER

PTHR12060. PTHR12060. 1 hit.

Pfam

PF02029. Caldesmon. 1 hit.
[Graphical view]

PRINTS

PR01083. LYMPHSPCIFIC.

ProtoNet

Search...

Other

ChiTaRS

LSP1. human.

EvolutionaryTrace

P33241.

GenomeRNAi

4046.

NextBio

15838.

SOURCE

Search...

Entry information

Entry name

LSP1_HUMAN

Accession

Primary (citable) accession number: P33241
Secondary accession number(s): B3KPP1 , B3KRR6, E9PBV6, E9PFP3, Q16096, Q53H48, Q6FHM3, Q9BUY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 29, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.