Reviewed, UniProtKB/Swiss-Prot P33241 (LSP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Lymphocyte-specific protein 1
Alternative name(s):
    52 kDa phosphoprotein
    Protein pp52
    Lymphocyte-specific antigen WP34
    47 kDa actin-binding protein

Gene names

Name:	LSP1
Synonyms:	WP34

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in mediating neutrophil activation and chemotaxis By similarity.
Subunit structure	Binds actin.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue specificity	Activated T-lymphocytes.
Post-translational modification	Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis By similarity.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell motion Ref.3 Traceable author statement. Source: ProtInc cellular defense response Ref.2 Traceable author statement. Source: ProtInc signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	actin cytoskeleton Ref.3 Traceable author statement. Source: ProtInc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	actin binding Ref.3 Traceable author statement. Source: ProtInc signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Lymphocyte-specific protein 1		PRO_0000084503
Amino acid modifications
	Modified residue	85	1	Phosphoserine By similarity
	Modified residue	175	1	Phosphothreonine By similarity
	Modified residue	177	1	Phosphoserine By similarity
	Modified residue	252	1	Phosphoserine; by MAPKAPK2 Ref.7 Ref.8
Natural variations
	Natural variant	100	1	A → T: dbSNP rs621679. Ref.5 Ref.6		VAR_011867
	Natural variant	229	1	Q → K: dbSNP rs1803928.		VAR_011868
Experimental info
	Sequence conflict	15	1	Missing in AAB29545. Ref.3
	Sequence conflict	24	1	S → T in CAA38971. Ref.2
	Sequence conflict	100 – 101	2	Missing in AAB29545. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P33241-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: F2A18533500611D9	FASTA	339	37,192
	10 20 30 40 50 60 MAEASSDPGA EEREELLGPT AQWSVEDEEE AVHEQCQHER DRQLQAQDEE GGGHVPERPK 70 80 90 100 110 120 QEMLLSLKPS EAPELDEDEG FGDWSQRPEQ RQQHEGAQGA LDSGEPPQCR SPEGEQEDRP 130 140 150 160 170 180 GLHAYEKEDS DEVHLEELSL SKEGPGPEDT VQDNLGAAGA EEEQEEHQKC QQPRTPSPLV 190 200 210 220 230 240 LEGTIEQSSP PLSPTTKLID RTESLNRSIE KSNSVKKSQP DLPISKIDQW LEQYTQAIET 250 260 270 280 290 300 AGRTPKLARQ ASIELPSMAV ASTKSRWETG EVQAQSAAKT PSCKDIVAGD MSKKSLWEQK 310 320 330 GGSKTSSTIK STPSGKRYKF VATGHGKYEK VLVEGGPAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human and mouse LSP1 genes code for highly conserved phosphoproteins." Jongstra-Bilen J., Young A.J., Chong R., Jongstra J. J. Immunol. 144:1104-1110(1990) [PubMed: 2295815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning and characterization of WP34, a phosphorylated human lymphocyte differentiation and activation antigen." Kadiyala R.K., McIntyre B.W., Krensky A.M. Eur. J. Immunol. 20:2417-2423(1990) [PubMed: 2174784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lymphocyte.
[3]	"The 47-kD protein increased in neutrophil actin dysfunction with 47- and 89-kD protein abnormalities is lymphocyte-specific protein." Howard T., Li Y., Torres M., Guerrero A., Coates T. Blood 83:231-241(1994) [PubMed: 8274738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-100. Tissue: Dermoid cancer.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-100. Tissue: Lymph.
[7]	"MAPKAPK2-mediated LSP1 phosphorylation and FMLP-induced neutrophil polarization." Wu Y., Zhan L., Ai Y., Hannigan M., Gaestel M., Huang C.-K., Madri J.A. Biochem. Biophys. Res. Commun. 358:170-175(2007) [PubMed: 17481585] [Abstract] Cited for: PHOSPHORYLATION AT SER-252 BY MAPKAPK2.
[8]	Carrascal M., Abian J. Submitted (JUN-2007) to UniProtKB Cited for: PHOSPHORYLATION AT SER-252, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M33552 mRNA. Translation: AAA59534.1.
X55188 mRNA. Translation: CAA38971.1.
S67783 mRNA. Translation: AAB29545.1.
CR541728 mRNA. Translation: CAG46529.1.
CR541754 mRNA. Translation: CAG46554.1.
AK222733 mRNA. Translation: BAD96453.1.
BC001785 mRNA. Translation: AAH01785.1.

IPI

IPI00013260.

PIR

A43542.

RefSeq

NP_001013271.1.
NP_001013273.1.
NP_002330.1.

UniGene

Hs.56729

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BH8	X-ray	1.65	C	249-258	[»]

ModBase

Search...

PTM databases

PhosphoSite

P33241.

2-D gel databases

SWISS-2DPAGE

P33241.

Proteomic databases

PeptideAtlas

P33241.

PRIDE

P33241.

Genome annotation databases

Ensembl

ENSG00000130592. Homo sapiens. [Contig view]

GeneID

4046.

KEGG

hsa:4046.

Organism-specific databases

GeneCards

GC11P001830.

H-InvDB

HIX0009349.

HGNC

HGNC:6707. LSP1.

HPA

CAB017781.

MIM

153432. gene.

PharmGKB

PA30472.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P33241.

HOVERGEN

P33241.

Enzyme and pathway databases

Pathway_Interaction_DB

p38_mk2pathway. p38 signaling mediated by MAPKAP kinases.

Gene expression databases

ArrayExpress

P33241.

Bgee

P33241.

CleanEx

HS_LSP1.

GermOnline

ENSG00000130592. Homo sapiens.

Family and domain databases

InterPro

IPR006018. Caldesmon_LSP.
IPR002211. Lymphspecific.
[Graphical view]

PANTHER

PTHR12060. Lymphspecific. 1 hit.

Pfam

PF02029. Caldesmon. 1 hit.
[Graphical view]

PRINTS

PR01083. LYMPHSPCIFIC.

ProtoNet

Search...

Other Resources

NextBio

15838.

SOURCE

Search...

Entry information

Entry name

LSP1_HUMAN

Accession

Primary (citable) accession number: P33241
Secondary accession number(s): Q16096 , Q53H48, Q6FHM3, Q9BUY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)