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P33241 (LSP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lymphocyte-specific protein 1
Alternative name(s):
47 kDa actin-binding protein
52 kDa phosphoprotein
Short name=pp52
Lymphocyte-specific antigen WP34
Gene names
Name:LSP1
Synonyms:WP34
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in mediating neutrophil activation and chemotaxis By similarity.

Subunit structure

Binds actin.

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue specificity

Activated T-lymphocytes.

Post-translational modification

Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis By similarity. Ref.9 Ref.10

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P33241-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P33241-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: P33241-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MAEASSDPGAEEREELLG → MAPIWSPPGR...SGPSTNCSPR
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 339339Lymphocyte-specific protein 1
PRO_0000084503

Amino acid modifications

Modified residue1751Phosphothreonine By similarity
Modified residue1771Phosphoserine By similarity
Modified residue1891Phosphoserine Ref.12
Modified residue2521Phosphoserine; by MAPKAPK2 Ref.9 Ref.10
Modified residue3271N6-acetyllysine Ref.13

Natural variations

Alternative sequence1 – 6262Missing in isoform 2.
VSP_045655
Alternative sequence1 – 1818MAEAS…EELLG → MAPIWSPPGRVSGCHLSSGP APGSAVGPWLGTPHPSLPLP LAPHKPPPPGLPGSAGQTSL PAQRECVFPGDAAVHQELCG LGFEECLGSIPQAHQCYLTN GPKRRKCSPRRRGRAPAWLC GGSPPCHQGLGHEHPSSGPS TNCSPR in isoform 3.
VSP_045983
Natural variant1001A → T. Ref.5 Ref.6 Ref.8
Corresponds to variant rs621679 [ dbSNP | Ensembl ].
VAR_011867
Natural variant1081Q → L.
Corresponds to variant rs11545725 [ dbSNP | Ensembl ].
VAR_061680
Natural variant2291Q → K.
Corresponds to variant rs1803928 [ dbSNP | Ensembl ].
VAR_011868

Experimental info

Sequence conflict151Missing in AAB29545. Ref.3
Sequence conflict241S → T in CAA38971. Ref.2
Sequence conflict100 – 1012Missing in AAB29545. Ref.3
Sequence conflict1481E → D in BAG52478. Ref.5
Isoform 3:
Sequence conflict311G → Q in BAG52478. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: F2A18533500611D9

FASTA33937,192
        10         20         30         40         50         60 
MAEASSDPGA EEREELLGPT AQWSVEDEEE AVHEQCQHER DRQLQAQDEE GGGHVPERPK 

        70         80         90        100        110        120 
QEMLLSLKPS EAPELDEDEG FGDWSQRPEQ RQQHEGAQGA LDSGEPPQCR SPEGEQEDRP 

       130        140        150        160        170        180 
GLHAYEKEDS DEVHLEELSL SKEGPGPEDT VQDNLGAAGA EEEQEEHQKC QQPRTPSPLV 

       190        200        210        220        230        240 
LEGTIEQSSP PLSPTTKLID RTESLNRSIE KSNSVKKSQP DLPISKIDQW LEQYTQAIET 

       250        260        270        280        290        300 
AGRTPKLARQ ASIELPSMAV ASTKSRWETG EVQAQSAAKT PSCKDIVAGD MSKKSLWEQK 

       310        320        330 
GGSKTSSTIK STPSGKRYKF VATGHGKYEK VLVEGGPAP 

« Hide

Isoform 2 [UniParc].

Checksum: DA869746FA7B7C6D
Show »

FASTA27730,213
Isoform 3 [UniParc].

Checksum: A3F09D8EECD570D6
Show »

FASTA46750,389

References

« Hide 'large scale' references
[1]"Human and mouse LSP1 genes code for highly conserved phosphoproteins."
Jongstra-Bilen J., Young A.J., Chong R., Jongstra J.
J. Immunol. 144:1104-1110(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Molecular cloning and characterization of WP34, a phosphorylated human lymphocyte differentiation and activation antigen."
Kadiyala R.K., McIntyre B.W., Krensky A.M.
Eur. J. Immunol. 20:2417-2423(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Lymphocyte.
[3]"The 47-kD protein increased in neutrophil actin dysfunction with 47- and 89-kD protein abnormalities is lymphocyte-specific protein."
Howard T., Li Y., Torres M., Guerrero A., Coates T.
Blood 83:231-241(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANT THR-100.
Tissue: Esophagus and Tongue.
[6]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-100.
Tissue: Dermoid cancer.
[7]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-100.
Tissue: Lymph.
[9]"MAPKAPK2-mediated LSP1 phosphorylation and FMLP-induced neutrophil polarization."
Wu Y., Zhan L., Ai Y., Hannigan M., Gaestel M., Huang C.-K., Madri J.A.
Biochem. Biophys. Res. Commun. 358:170-175(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-252 BY MAPKAPK2.
[10]Carrascal M., Abian J.
Submitted (JUN-2007) to UniProtKB
Cited for: PHOSPHORYLATION AT SER-252, IDENTIFICATION BY MASS SPECTROMETRY.
[11]"Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."
Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J.
J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: T-cell.
[12]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[13]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-327, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M33552 mRNA. Translation: AAA59534.1.
X55188 mRNA. Translation: CAA38971.1.
S67783 mRNA. Translation: AAB29545.1.
CR541728 mRNA. Translation: CAG46529.1.
CR541754 mRNA. Translation: CAG46554.1.
AK056576 mRNA. Translation: BAG51753.1.
AK092071 mRNA. Translation: BAG52478.1.
AK222733 mRNA. Translation: BAD96453.1.
AC051649 Genomic DNA. No translation available.
BC001785 mRNA. Translation: AAH01785.1.
CCDSCCDS31334.1. [P33241-1]
CCDS31335.1. [P33241-2]
CCDS58110.1. [P33241-3]
PIRA43542.
RefSeqNP_001013271.1. NM_001013253.1. [P33241-2]
NP_001013272.1. NM_001013254.1. [P33241-2]
NP_001013273.1. NM_001013255.1. [P33241-2]
NP_001229861.1. NM_001242932.1. [P33241-3]
NP_001275934.1. NM_001289005.1. [P33241-2]
NP_002330.1. NM_002339.2. [P33241-1]
XP_005252983.1. XM_005252926.1. [P33241-2]
XP_005252984.1. XM_005252927.1. [P33241-2]
UniGeneHs.56729.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3BH8X-ray1.65C249-258[»]
ProteinModelPortalP33241.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110224. 6 interactions.
STRING9606.ENSP00000308383.

PTM databases

PhosphoSiteP33241.

Polymorphism databases

DMDM462553.

2D gel databases

SWISS-2DPAGEP33241.

Proteomic databases

MaxQBP33241.
PaxDbP33241.
PeptideAtlasP33241.
PRIDEP33241.

Protocols and materials databases

DNASU4046.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000311604; ENSP00000308383; ENSG00000130592. [P33241-1]
ENST00000381775; ENSP00000371194; ENSG00000130592. [P33241-3]
ENST00000405957; ENSP00000383932; ENSG00000130592. [P33241-2]
ENST00000406638; ENSP00000384022; ENSG00000130592. [P33241-2]
GeneID4046.
KEGGhsa:4046.
UCSCuc001lui.3. human. [P33241-1]

Organism-specific databases

CTD4046.
GeneCardsGC11P001874.
HGNCHGNC:6707. LSP1.
HPACAB017781.
HPA019693.
MIM153432. gene.
neXtProtNX_P33241.
PharmGKBPA30472.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG302084.
HOGENOMHOG000231050.
HOVERGENHBG001610.
KOK14957.
OMARLTAQWS.
OrthoDBEOG7FNC7W.
PhylomeDBP33241.
TreeFamTF336257.

Enzyme and pathway databases

SignaLinkP33241.

Gene expression databases

ArrayExpressP33241.
BgeeP33241.
CleanExHS_LSP1.
GenevestigatorP33241.

Family and domain databases

InterProIPR006018. Caldesmon_LSP.
IPR002211. Lymphspecific.
[Graphical view]
PANTHERPTHR18949. PTHR18949. 1 hit.
PfamPF02029. Caldesmon. 1 hit.
[Graphical view]
PRINTSPR01083. LYMPHSPCIFIC.
ProtoNetSearch...

Other

ChiTaRSLSP1. human.
EvolutionaryTraceP33241.
GeneWikiLSP1.
GenomeRNAi4046.
NextBio15838.
PROP33241.
SOURCESearch...

Entry information

Entry nameLSP1_HUMAN
AccessionPrimary (citable) accession number: P33241
Secondary accession number(s): B3KPP1 expand/collapse secondary AC list , B3KRR6, E9PBV6, E9PFP3, Q16096, Q53H48, Q6FHM3, Q9BUY8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 9, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM