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P33241

- LSP1_HUMAN

UniProt

P33241 - LSP1_HUMAN

Protein

Lymphocyte-specific protein 1

Gene

LSP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    May play a role in mediating neutrophil activation and chemotaxis.By similarity

    GO - Molecular functioni

    1. actin binding Source: ProtInc
    2. signal transducer activity Source: InterPro

    GO - Biological processi

    1. cellular component movement Source: ProtInc
    2. cellular defense response Source: ProtInc

    Enzyme and pathway databases

    SignaLinkiP33241.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lymphocyte-specific protein 1
    Alternative name(s):
    47 kDa actin-binding protein
    52 kDa phosphoprotein
    Short name:
    pp52
    Lymphocyte-specific antigen WP34
    Gene namesi
    Name:LSP1
    Synonyms:WP34
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:6707. LSP1.

    Subcellular locationi

    GO - Cellular componenti

    1. actin cytoskeleton Source: ProtInc
    2. extracellular vesicular exosome Source: UniProt
    3. membrane Source: UniProtKB
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30472.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 339339Lymphocyte-specific protein 1PRO_0000084503Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei175 – 1751PhosphothreonineBy similarity
    Modified residuei177 – 1771PhosphoserineBy similarity
    Modified residuei189 – 1891Phosphoserine1 Publication
    Modified residuei252 – 2521Phosphoserine; by MAPKAPK22 Publications
    Modified residuei327 – 3271N6-acetyllysine1 Publication

    Post-translational modificationi

    Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP33241.
    PaxDbiP33241.
    PeptideAtlasiP33241.
    PRIDEiP33241.

    2D gel databases

    SWISS-2DPAGEP33241.

    PTM databases

    PhosphoSiteiP33241.

    Expressioni

    Tissue specificityi

    Activated T-lymphocytes.

    Gene expression databases

    ArrayExpressiP33241.
    BgeeiP33241.
    CleanExiHS_LSP1.
    GenevestigatoriP33241.

    Organism-specific databases

    HPAiCAB017781.
    HPA019693.

    Interactioni

    Subunit structurei

    Binds actin.

    Protein-protein interaction databases

    BioGridi110224. 6 interactions.
    STRINGi9606.ENSP00000308383.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3BH8X-ray1.65C249-258[»]
    ProteinModelPortaliP33241.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP33241.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG302084.
    HOGENOMiHOG000231050.
    HOVERGENiHBG001610.
    KOiK14957.
    OMAiRLTAQWS.
    OrthoDBiEOG7FNC7W.
    PhylomeDBiP33241.
    TreeFamiTF336257.

    Family and domain databases

    InterProiIPR006018. Caldesmon_LSP.
    IPR002211. Lymphspecific.
    [Graphical view]
    PANTHERiPTHR18949. PTHR18949. 1 hit.
    PfamiPF02029. Caldesmon. 1 hit.
    [Graphical view]
    PRINTSiPR01083. LYMPHSPCIFIC.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P33241-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEASSDPGA EEREELLGPT AQWSVEDEEE AVHEQCQHER DRQLQAQDEE    50
    GGGHVPERPK QEMLLSLKPS EAPELDEDEG FGDWSQRPEQ RQQHEGAQGA 100
    LDSGEPPQCR SPEGEQEDRP GLHAYEKEDS DEVHLEELSL SKEGPGPEDT 150
    VQDNLGAAGA EEEQEEHQKC QQPRTPSPLV LEGTIEQSSP PLSPTTKLID 200
    RTESLNRSIE KSNSVKKSQP DLPISKIDQW LEQYTQAIET AGRTPKLARQ 250
    ASIELPSMAV ASTKSRWETG EVQAQSAAKT PSCKDIVAGD MSKKSLWEQK 300
    GGSKTSSTIK STPSGKRYKF VATGHGKYEK VLVEGGPAP 339
    Length:339
    Mass (Da):37,192
    Last modified:February 1, 1994 - v1
    Checksum:iF2A18533500611D9
    GO
    Isoform 2 (identifier: P33241-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-62: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:277
    Mass (Da):30,213
    Checksum:iDA869746FA7B7C6D
    GO
    Isoform 3 (identifier: P33241-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-18: MAEASSDPGAEEREELLG → MAPIWSPPGR...SGPSTNCSPR

    Note: No experimental confirmation available.Curated

    Show »
    Length:467
    Mass (Da):50,389
    Checksum:iA3F09D8EECD570D6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti15 – 151Missing in AAB29545. (PubMed:8274738)Curated
    Sequence conflicti24 – 241S → T in CAA38971. (PubMed:2174784)Curated
    Sequence conflicti100 – 1012Missing in AAB29545. (PubMed:8274738)Curated
    Sequence conflicti148 – 1481E → D in BAG52478. (PubMed:14702039)Curated
    Isoform 3 (identifier: P33241-3)
    Sequence conflicti31 – 311G → Q in BAG52478. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti100 – 1001A → T.3 Publications
    Corresponds to variant rs621679 [ dbSNP | Ensembl ].
    VAR_011867
    Natural varianti108 – 1081Q → L.
    Corresponds to variant rs11545725 [ dbSNP | Ensembl ].
    VAR_061680
    Natural varianti229 – 2291Q → K.
    Corresponds to variant rs1803928 [ dbSNP | Ensembl ].
    VAR_011868

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 6262Missing in isoform 2. 1 PublicationVSP_045655Add
    BLAST
    Alternative sequencei1 – 1818MAEAS…EELLG → MAPIWSPPGRVSGCHLSSGP APGSAVGPWLGTPHPSLPLP LAPHKPPPPGLPGSAGQTSL PAQRECVFPGDAAVHQELCG LGFEECLGSIPQAHQCYLTN GPKRRKCSPRRRGRAPAWLC GGSPPCHQGLGHEHPSSGPS TNCSPR in isoform 3. 1 PublicationVSP_045983Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33552 mRNA. Translation: AAA59534.1.
    X55188 mRNA. Translation: CAA38971.1.
    S67783 mRNA. Translation: AAB29545.1.
    CR541728 mRNA. Translation: CAG46529.1.
    CR541754 mRNA. Translation: CAG46554.1.
    AK056576 mRNA. Translation: BAG51753.1.
    AK092071 mRNA. Translation: BAG52478.1.
    AK222733 mRNA. Translation: BAD96453.1.
    AC051649 Genomic DNA. No translation available.
    BC001785 mRNA. Translation: AAH01785.1.
    CCDSiCCDS31334.1. [P33241-1]
    CCDS31335.1. [P33241-2]
    CCDS58110.1. [P33241-3]
    PIRiA43542.
    RefSeqiNP_001013271.1. NM_001013253.1. [P33241-2]
    NP_001013272.1. NM_001013254.1. [P33241-2]
    NP_001013273.1. NM_001013255.1. [P33241-2]
    NP_001229861.1. NM_001242932.1. [P33241-3]
    NP_001275934.1. NM_001289005.1. [P33241-2]
    NP_002330.1. NM_002339.2. [P33241-1]
    XP_005252983.1. XM_005252926.1. [P33241-2]
    XP_005252984.1. XM_005252927.1. [P33241-2]
    UniGeneiHs.56729.

    Genome annotation databases

    EnsembliENST00000311604; ENSP00000308383; ENSG00000130592. [P33241-1]
    ENST00000381775; ENSP00000371194; ENSG00000130592. [P33241-3]
    ENST00000405957; ENSP00000383932; ENSG00000130592. [P33241-2]
    ENST00000406638; ENSP00000384022; ENSG00000130592. [P33241-2]
    GeneIDi4046.
    KEGGihsa:4046.
    UCSCiuc001lui.3. human. [P33241-1]

    Polymorphism databases

    DMDMi462553.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33552 mRNA. Translation: AAA59534.1 .
    X55188 mRNA. Translation: CAA38971.1 .
    S67783 mRNA. Translation: AAB29545.1 .
    CR541728 mRNA. Translation: CAG46529.1 .
    CR541754 mRNA. Translation: CAG46554.1 .
    AK056576 mRNA. Translation: BAG51753.1 .
    AK092071 mRNA. Translation: BAG52478.1 .
    AK222733 mRNA. Translation: BAD96453.1 .
    AC051649 Genomic DNA. No translation available.
    BC001785 mRNA. Translation: AAH01785.1 .
    CCDSi CCDS31334.1. [P33241-1 ]
    CCDS31335.1. [P33241-2 ]
    CCDS58110.1. [P33241-3 ]
    PIRi A43542.
    RefSeqi NP_001013271.1. NM_001013253.1. [P33241-2 ]
    NP_001013272.1. NM_001013254.1. [P33241-2 ]
    NP_001013273.1. NM_001013255.1. [P33241-2 ]
    NP_001229861.1. NM_001242932.1. [P33241-3 ]
    NP_001275934.1. NM_001289005.1. [P33241-2 ]
    NP_002330.1. NM_002339.2. [P33241-1 ]
    XP_005252983.1. XM_005252926.1. [P33241-2 ]
    XP_005252984.1. XM_005252927.1. [P33241-2 ]
    UniGenei Hs.56729.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3BH8 X-ray 1.65 C 249-258 [» ]
    ProteinModelPortali P33241.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110224. 6 interactions.
    STRINGi 9606.ENSP00000308383.

    PTM databases

    PhosphoSitei P33241.

    Polymorphism databases

    DMDMi 462553.

    2D gel databases

    SWISS-2DPAGE P33241.

    Proteomic databases

    MaxQBi P33241.
    PaxDbi P33241.
    PeptideAtlasi P33241.
    PRIDEi P33241.

    Protocols and materials databases

    DNASUi 4046.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000311604 ; ENSP00000308383 ; ENSG00000130592 . [P33241-1 ]
    ENST00000381775 ; ENSP00000371194 ; ENSG00000130592 . [P33241-3 ]
    ENST00000405957 ; ENSP00000383932 ; ENSG00000130592 . [P33241-2 ]
    ENST00000406638 ; ENSP00000384022 ; ENSG00000130592 . [P33241-2 ]
    GeneIDi 4046.
    KEGGi hsa:4046.
    UCSCi uc001lui.3. human. [P33241-1 ]

    Organism-specific databases

    CTDi 4046.
    GeneCardsi GC11P001874.
    HGNCi HGNC:6707. LSP1.
    HPAi CAB017781.
    HPA019693.
    MIMi 153432. gene.
    neXtProti NX_P33241.
    PharmGKBi PA30472.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG302084.
    HOGENOMi HOG000231050.
    HOVERGENi HBG001610.
    KOi K14957.
    OMAi RLTAQWS.
    OrthoDBi EOG7FNC7W.
    PhylomeDBi P33241.
    TreeFami TF336257.

    Enzyme and pathway databases

    SignaLinki P33241.

    Miscellaneous databases

    ChiTaRSi LSP1. human.
    EvolutionaryTracei P33241.
    GeneWikii LSP1.
    GenomeRNAii 4046.
    NextBioi 15838.
    PROi P33241.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P33241.
    Bgeei P33241.
    CleanExi HS_LSP1.
    Genevestigatori P33241.

    Family and domain databases

    InterProi IPR006018. Caldesmon_LSP.
    IPR002211. Lymphspecific.
    [Graphical view ]
    PANTHERi PTHR18949. PTHR18949. 1 hit.
    Pfami PF02029. Caldesmon. 1 hit.
    [Graphical view ]
    PRINTSi PR01083. LYMPHSPCIFIC.
    ProtoNeti Search...

    Publicationsi

    1. "Human and mouse LSP1 genes code for highly conserved phosphoproteins."
      Jongstra-Bilen J., Young A.J., Chong R., Jongstra J.
      J. Immunol. 144:1104-1110(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Molecular cloning and characterization of WP34, a phosphorylated human lymphocyte differentiation and activation antigen."
      Kadiyala R.K., McIntyre B.W., Krensky A.M.
      Eur. J. Immunol. 20:2417-2423(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Lymphocyte.
    3. "The 47-kD protein increased in neutrophil actin dysfunction with 47- and 89-kD protein abnormalities is lymphocyte-specific protein."
      Howard T., Li Y., Torres M., Guerrero A., Coates T.
      Blood 83:231-241(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANT THR-100.
      Tissue: Esophagus and Tongue.
    6. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-100.
      Tissue: Dermoid cancer.
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-100.
      Tissue: Lymph.
    9. "MAPKAPK2-mediated LSP1 phosphorylation and FMLP-induced neutrophil polarization."
      Wu Y., Zhan L., Ai Y., Hannigan M., Gaestel M., Huang C.-K., Madri J.A.
      Biochem. Biophys. Res. Commun. 358:170-175(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-252 BY MAPKAPK2.
    10. Carrascal M., Abian J.
      Submitted (JUN-2007) to UniProtKB
      Cited for: PHOSPHORYLATION AT SER-252, IDENTIFICATION BY MASS SPECTROMETRY.
    11. "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."
      Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J.
      J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: T-cell.
    12. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    13. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-327, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLSP1_HUMAN
    AccessioniPrimary (citable) accession number: P33241
    Secondary accession number(s): B3KPP1
    , B3KRR6, E9PBV6, E9PFP3, Q16096, Q53H48, Q6FHM3, Q9BUY8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3