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Protein

Cleavage stimulation factor subunit 2

Gene

CSTF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity).By similarity1 Publication

GO - Molecular functioni

  • mRNA binding Source: GO_Central
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • RNA binding Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000101811-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-6784531. tRNA processing in the nucleus.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.
R-HSA-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage stimulation factor subunit 2
Alternative name(s):
CF-1 64 kDa subunit
Cleavage stimulation factor 64 kDa subunit
Short name:
CSTF 64 kDa subunit
Short name:
CstF-64
Gene namesi
Name:CSTF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:2484. CSTF2.

Subcellular locationi

GO - Cellular componenti

  • cleavage body Source: UniProtKB
  • mRNA cleavage and polyadenylation specificity factor complex Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1478.
OpenTargetsiENSG00000101811.
PharmGKBiPA26986.

Polymorphism and mutation databases

BioMutaiCSTF2.
DMDMi461847.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000815311 – 577Cleavage stimulation factor subunit 2Add BLAST577

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineCombined sources1
Cross-linki189Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei308Omega-N-methylarginineCombined sources1
Modified residuei468Omega-N-methylarginineCombined sources1
Modified residuei475Omega-N-methylarginineCombined sources1
Modified residuei518PhosphoserineCombined sources1
Modified residuei524PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP33240.
MaxQBiP33240.
PaxDbiP33240.
PeptideAtlasiP33240.
PRIDEiP33240.

PTM databases

iPTMnetiP33240.
PhosphoSitePlusiP33240.
SwissPalmiP33240.

Expressioni

Inductioni

Up-regulated during the G0 to S phase transition.1 Publication

Gene expression databases

BgeeiENSG00000101811.
CleanExiHS_CSTF2.
ExpressionAtlasiP33240. baseline and differential.
GenevisibleiP33240. HS.

Organism-specific databases

HPAiCAB004680.
HPA000427.

Interactioni

Subunit structurei

The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1. Interacts with HSF1 in heat-stressed cells. Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BARD1Q997288EBI-711360,EBI-473181
CPSF2Q9P2I02EBI-711360,EBI-1043224
HGSO149643EBI-711360,EBI-740220
PARNO954535EBI-711360,EBI-372832
SPAG8Q999323EBI-711360,EBI-954419
SS18L1O751773EBI-711360,EBI-744674
SUB1P539993EBI-711374,EBI-998260
TFGQ927345EBI-711360,EBI-357061
UBQLN1Q9UMX06EBI-711360,EBI-741480
UBQLN1Q9UMX0-23EBI-711360,EBI-10173939
ZNF341Q9BYN73EBI-711360,EBI-9089622

Protein-protein interaction databases

BioGridi107860. 77 interactors.
DIPiDIP-36129N.
IntActiP33240. 35 interactors.
MINTiMINT-1375144.
STRINGi9606.ENSP00000362063.

Structurei

Secondary structure

1577
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 15Combined sources4
Beta strandi18 – 22Combined sources5
Helixi29 – 37Combined sources9
Beta strandi43 – 50Combined sources8
Turni51 – 54Combined sources4
Beta strandi55 – 63Combined sources9
Helixi67 – 76Combined sources10
Beta strandi77 – 81Combined sources5
Beta strandi83 – 85Combined sources3
Beta strandi87 – 91Combined sources5
Helixi97 – 104Combined sources8
Helixi532 – 535Combined sources4
Helixi537 – 545Combined sources9
Helixi549 – 553Combined sources5
Helixi557 – 560Combined sources4
Helixi562 – 571Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P1TNMR-A8-111[»]
2J8PNMR-A531-577[»]
ProteinModelPortaliP33240.
SMRiP33240.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33240.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 94RRMPROSITE-ProRule annotationAdd BLAST79
Repeati410 – 4141; approximate5
Repeati415 – 41925
Repeati420 – 42435
Repeati425 – 4294; approximate5
Repeati430 – 4345; approximate5
Repeati435 – 43965
Repeati440 – 44475
Repeati445 – 44985
Repeati450 – 45495
Repeati455 – 45910; approximate5
Repeati460 – 464115
Repeati465 – 46912; approximate5

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 248Interactions with CSTF3 and SYMPKAdd BLAST141
Regioni410 – 46912 X 5 AA tandem repeats of M-E-A-R-[AG]Add BLAST60
Regioni514 – 577Interaction with RPO2TC11 PublicationAdd BLAST64

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi198 – 409Gly/Pro-richAdd BLAST212
Compositional biasi470 – 526Gly/Pro-richAdd BLAST57

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0108. Eukaryota.
ENOG410XQBV. LUCA.
GeneTreeiENSGT00820000127074.
HOGENOMiHOG000214373.
HOVERGENiHBG051145.
InParanoidiP33240.
KOiK14407.
PhylomeDBiP33240.
TreeFamiTF314948.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033105. CSTF2.
IPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23139:SF57. PTHR23139:SF57. 1 hit.
PfamiPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P33240-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGLTVRDPA VDRSLRSVFV GNIPYEATEE QLKDIFSEVG PVVSFRLVYD
60 70 80 90 100
RETGKPKGYG FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE
110 120 130 140 150
LKSLGTGAPV IESPYGETIS PEDAPESISK AVASLPPEQM FELMKQMKLC
160 170 180 190 200
VQNSPQEARN MLLQNPQLAY ALLQAQVVMR IVDPEIALKI LHRQTNIPTL
210 220 230 240 250
IAGNPQPVHG AGPGSGSNVS MNQQNPQAPQ AQSLGGMHVN GAPPLMQASM
260 270 280 290 300
QGGVPAPGQM PAAVTGPGPG SLAPGGGMQA QVGMPGSGPV SMERGQVPMQ
310 320 330 340 350
DPRAAMQRGS LPANVPTPRG LLGDAPNDPR GGTLLSVTGE VEPRGYLGPP
360 370 380 390 400
HQGPPMHHVP GHESRGPPPH ELRGGPLPEP RPLMAEPRGP MLDQRGPPLD
410 420 430 440 450
GRGGRDPRGI DARGMEARAM EARGLDARGL EARAMEARAM EARAMEARAM
460 470 480 490 500
EARAMEVRGM EARGMDTRGP VPGPRGPIPS GMQGPSPINM GAVVPQGSRQ
510 520 530 540 550
VPVMQGTGMQ GASIQGGSQP GGFSPGQNQV TPQDHEKAAL IMQVLQLTAD
560 570
QIAMLPPEQR QSILILKEQI QKSTGAP
Length:577
Mass (Da):60,959
Last modified:February 1, 1994 - v1
Checksum:iB76595E9BF8FABBD
GO
Isoform 2 (identifier: P33240-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     235-251: Missing.

Note: No experimental confirmation available.
Show »
Length:560
Mass (Da):59,251
Checksum:iD76823A0FF6363B8
GO

Sequence cautioni

The sequence CAI42681 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014841235 – 251Missing in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85085 mRNA. Translation: AAA35724.1.
BT009778 mRNA. Translation: AAP88780.1.
Z83819, Z95327 Genomic DNA. Translation: CAB06072.2.
Z95327, Z83819 Genomic DNA. Translation: CAI42680.1.
Z95327 Genomic DNA. Translation: CAI42681.1. Sequence problems.
BC017712 mRNA. Translation: AAH17712.1.
BC033135 mRNA. Translation: AAH33135.1.
CCDSiCCDS14473.1. [P33240-1]
PIRiA40220.
RefSeqiNP_001293138.1. NM_001306209.1. [P33240-2]
NP_001316.1. NM_001325.2. [P33240-1]
UniGeneiHs.132370.

Genome annotation databases

EnsembliENST00000372972; ENSP00000362063; ENSG00000101811. [P33240-1]
GeneIDi1478.
KEGGihsa:1478.
UCSCiuc004egh.4. human. [P33240-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85085 mRNA. Translation: AAA35724.1.
BT009778 mRNA. Translation: AAP88780.1.
Z83819, Z95327 Genomic DNA. Translation: CAB06072.2.
Z95327, Z83819 Genomic DNA. Translation: CAI42680.1.
Z95327 Genomic DNA. Translation: CAI42681.1. Sequence problems.
BC017712 mRNA. Translation: AAH17712.1.
BC033135 mRNA. Translation: AAH33135.1.
CCDSiCCDS14473.1. [P33240-1]
PIRiA40220.
RefSeqiNP_001293138.1. NM_001306209.1. [P33240-2]
NP_001316.1. NM_001325.2. [P33240-1]
UniGeneiHs.132370.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1P1TNMR-A8-111[»]
2J8PNMR-A531-577[»]
ProteinModelPortaliP33240.
SMRiP33240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107860. 77 interactors.
DIPiDIP-36129N.
IntActiP33240. 35 interactors.
MINTiMINT-1375144.
STRINGi9606.ENSP00000362063.

PTM databases

iPTMnetiP33240.
PhosphoSitePlusiP33240.
SwissPalmiP33240.

Polymorphism and mutation databases

BioMutaiCSTF2.
DMDMi461847.

Proteomic databases

EPDiP33240.
MaxQBiP33240.
PaxDbiP33240.
PeptideAtlasiP33240.
PRIDEiP33240.

Protocols and materials databases

DNASUi1478.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372972; ENSP00000362063; ENSG00000101811. [P33240-1]
GeneIDi1478.
KEGGihsa:1478.
UCSCiuc004egh.4. human. [P33240-1]

Organism-specific databases

CTDi1478.
DisGeNETi1478.
GeneCardsiCSTF2.
HGNCiHGNC:2484. CSTF2.
HPAiCAB004680.
HPA000427.
MIMi300907. gene.
neXtProtiNX_P33240.
OpenTargetsiENSG00000101811.
PharmGKBiPA26986.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0108. Eukaryota.
ENOG410XQBV. LUCA.
GeneTreeiENSGT00820000127074.
HOGENOMiHOG000214373.
HOVERGENiHBG051145.
InParanoidiP33240.
KOiK14407.
PhylomeDBiP33240.
TreeFamiTF314948.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000101811-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-6784531. tRNA processing in the nucleus.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.
R-HSA-77595. Processing of Intronless Pre-mRNAs.

Miscellaneous databases

ChiTaRSiCSTF2. human.
EvolutionaryTraceiP33240.
GeneWikiiCSTF2.
GenomeRNAii1478.
PROiP33240.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101811.
CleanExiHS_CSTF2.
ExpressionAtlasiP33240. baseline and differential.
GenevisibleiP33240. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033105. CSTF2.
IPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23139:SF57. PTHR23139:SF57. 1 hit.
PfamiPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSTF2_HUMAN
AccessioniPrimary (citable) accession number: P33240
Secondary accession number(s): Q5H951, Q6LA74, Q8N502
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 167 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.