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Protein

Interferon-induced GTP-binding protein Mx1

Gene

MX1

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Interferon-induced dynamin-like GTPase with antiviral activity.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 79GTPSequence analysis8
Nucleotide bindingi173 – 177GTPSequence analysis5
Nucleotide bindingi242 – 245GTPSequence analysis4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-induced GTP-binding protein Mx1
Alternative name(s):
Myxoma resistance protein 1
Myxovirus resistance protein 1
Oligodendrocyte GTP-binding protein
Cleaved into the following chain:
Gene namesi
Name:MX1
Synonyms:MX
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm By similarity
  • Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cytoplasmperinuclear region By similarity

  • Note: Binds preferentially to negatively charged phospholipids.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002065961 – 654Interferon-induced GTP-binding protein Mx1Add BLAST654
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00003829492 – 654Interferon-induced GTP-binding protein Mx1, N-terminally processedAdd BLAST653

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

PRIDEiP33237.

Expressioni

Inductioni

By type I and type III interferons.2 Publications

Interactioni

Subunit structurei

Homooligomer. Oligomerizes into multimeric filamentous or ring-like structures by virtue of its stalk domain. Oligomerization is critical for GTPase activity, protein stability, and recognition of viral target structures (By similarity). Interacts with TRPC1, TRPC3, TRPC4, TRPC5, TRPC6 and TRPC7 (By similarity). Interacts with HSPA5 (By similarity). Interacts with DDX39A and DDX39B (By similarity). Interacts with TUBB/TUBB5.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliP33237.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 335Dynamin-type GAdd BLAST274
Domaini566 – 654GEDPROSITE-ProRule annotationAdd BLAST89

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 335GTPase domainBy similarityAdd BLAST272
Regioni336 – 361Bundle signaling element (BSE)By similarityAdd BLAST26
Regioni361 – 528Middle domainBy similarityAdd BLAST168
Regioni362 – 624StalkBy similarityAdd BLAST263
Regioni549 – 552Critical for lipid-bindingBy similarity4

Domaini

The C-terminal GTPase effector domain (GED) is involved in oligomerization and viral target recognition.By similarity
The middle domain mediates self-assembly and oligomerization.By similarity

Sequence similaritiesi

Contains 1 GED domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG008788.
KOiK14754.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33237-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLSDLDIKE PDSPESGLNG SDDMVREHET ESKGNLYSQY EEKVRPCIDL
60 70 80 90 100
IDSLRALGVE QDLALPAIAV IGDQSSGKSS VLEALSGVAL PRGSGIVTRC
110 120 130 140 150
PLVLRLKKLE KEGEWKGKVS FLDREIEISD ASQVEKEISE AQIAIAGEGM
160 170 180 190 200
GISHELISLE VSSPHVPDLT LIDLPGITRV AVGNQPHDIE YQIKSLIRKY
210 220 230 240 250
ILRQETINLV VVPANVDIAT TEALRMAQDV DPQGDRTIGI LTKPDLVDKG
260 270 280 290 300
TEDKVVDVVR NLVFHLKKGY MIVKCRGQQE IQHRLSLDKA LQRERIFFED
310 320 330 340 350
HTHFRDLLEE GRATIPCLAE RLTNELIMHI CKTLPLLENQ IKETHQRITE
360 370 380 390 400
ELQKYGKDIP EEESEKMFSL IEKIDTFNKE IISTIEGEEH VGQYDSRLFT
410 420 430 440 450
KVRAEFCKWS AVVEKNFEKG HEAIRKEIKQ FENRYRGREL PGFVNYKTFE
460 470 480 490 500
IIIKKQVIVL EEPAVDMLHT VTDIIRNTFT EVSGKHFSEF FNLHRTAKSK
510 520 530 540 550
IEDIRLEQEN EAEKSIRLHF QMEQLVYCQD QVYRRALQQV REKEAEEEKK
560 570 580 590 600
KKSNHYYQSE DSEPSTAEIF QHLMAYHQEV STRISSHIPL IIQFFVLRTY
610 620 630 640 650
GEQLKKSMLQ LLQDKDQYDW LLKERTDTRD KRKFLKERLE RLSRARQRLA

KFPG
Length:654
Mass (Da):75,573
Last modified:February 1, 1994 - v1
Checksum:iCF06250CA7456E04
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45R → L in AAK94466 (PubMed:11754216).Curated1
Sequence conflicti275C → S in AAK94466 (PubMed:11754216).Curated1
Sequence conflicti297F → I in AAK94466 (PubMed:11754216).Curated1
Sequence conflicti352L → F in AAK94466 (PubMed:11754216).Curated1
Sequence conflicti390H → L in AAK94466 (PubMed:11754216).Curated1
Sequence conflicti553S → K in AAK94466 (PubMed:11754216).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66093 mRNA. Translation: CAA46888.1.
AF399856 mRNA. Translation: AAK94466.1.
PIRiS21552.
RefSeqiNP_001009753.1. NM_001009753.1.
UniGeneiOar.62.

Genome annotation databases

GeneIDi443146.
KEGGioas:443146.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66093 mRNA. Translation: CAA46888.1.
AF399856 mRNA. Translation: AAK94466.1.
PIRiS21552.
RefSeqiNP_001009753.1. NM_001009753.1.
UniGeneiOar.62.

3D structure databases

ProteinModelPortaliP33237.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP33237.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi443146.
KEGGioas:443146.

Organism-specific databases

CTDi4599.

Phylogenomic databases

HOVERGENiHBG008788.
KOiK14754.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR000375. Dynamin_central.
IPR001401. Dynamin_GTPase.
IPR019762. Dynamin_GTPase_CS.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR003130. GED.
IPR020850. GED_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PfamiPF01031. Dynamin_M. 1 hit.
PF00350. Dynamin_N. 1 hit.
PF02212. GED. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
SM00302. GED. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00410. G_DYNAMIN_1. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
PS51388. GED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMX1_SHEEP
AccessioniPrimary (citable) accession number: P33237
Secondary accession number(s): Q95MD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 5, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.