P33232 (LLDD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase [cytochrome]
EC=1.1.2.3

Gene names

Name:	lldD
Synonyms:	lctD
Ordered Locus Names:	b3605, JW3580

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+. HAMAP MF_01559
Cofactor	FMN.
Induction	By L-lactate; aerobically. HAMAP MF_01559
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-fucose catabolic process Inferred from expression pattern. Source: EcoCyc aerobic respiration Inferred from expression pattern Ref.1. Source: EcoCyc lactate metabolic process Inferred from mutant phenotype Ref.1. Source: EcoCyc
Cellular component	plasma membrane Inferred from direct assay. Source: EcoCyc
Molecular function	FMN binding Inferred from direct assay. Source: EcoCyc L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yfgL	P77774	1	EBI-553210,EBI-907297

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	L-lactate dehydrogenase [cytochrome] HAMAP MF_01559		PRO_0000206335
Regions
	Domain	1 – 380	380	FMN hydroxy acid dehydrogenase
	Nucleotide binding	306 – 330	25	FMN By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	FMN By similarity
	Binding site	278	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	P33232 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: CA0D8E308713BF83	FASTA	396	42,728
	10 20 30 40 50 60 MIISAASDYR AAAQRILPPF LFHYMDGGAY SEYTLRRNVE DLSEVALRQR ILKNMSDLSL 70 80 90 100 110 120 ETTLFNEKLS MPVALAPVGL CGMYARRGEV QAAKAADAHG IPFTLSTVSV CPIEEVAPAI 130 140 150 160 170 180 KRPMWFQLYV LRDRGFMRNA LERAKAAGCS TLVFTVDMPT PGARYRDAHS GMSGPNAAMR 190 200 210 220 230 240 RYLQAVTHPQ WAWDVGLNGR PHDLGNISAY LGKPTGLEDY IGWLGNNFDP SISWKDLEWI 250 260 270 280 290 300 RDFWDGPMVI KGILDPEDAR DAVRFGADGI VVSNHGGRQL DGVLSSARAL PAIADAVKGD 310 320 330 340 350 360 IAILADSGIR NGLDVVRMIA LGADTVLLGR AFLYALATAG QAGVANLLNL IEKEMKVAMT 370 380 390 LTGAKSISEI TQDSLVQGLG KELPAALAPM AKGNAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three overlapping lct genes involved in L-lactate utilization by Escherichia coli." Dong J.M., Taylor J.S., Latour D.J., Iuchi S., Lin E.C.C. J. Bacteriol. 175:6671-6678(1993) [PubMed: 8407843] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L13970 Unassigned DNA. Translation: AAA03585.1. U00039 Genomic DNA. Translation: AAB18582.1. U00096 Genomic DNA. Translation: AAC76629.1. AP009048 Genomic DNA. Translation: BAE77687.1.
PIR	C49904.
RefSeq	NP_418062.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P33232.
SMR	P33232. Positions 3-377.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10108N.
IntAct	P33232. 14 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000928; EBESCP00000000928; EBESCG00000000766. EBESCT00000000929; EBESCP00000000929; EBESCG00000000766. EBESCT00000014840; EBESCP00000014131; EBESCG00000013900.
GeneID	948121.
GenomeReviews	Gene locus JW3580 in contig AP009048_GR. Gene locus b3605 in contig U00096_GR.
KEGG	ecj:JW3580. eco:b3605.
PATRIC	32122695. VBIEscCol129921_3724.
Organism-specific databases
EchoBASE	EB1906.
EcoGene	EG11963. lldD.
Phylogenomic databases
eggNOG	COG1304.
GeneTree	EBGT00050000009653.
HOGENOM	HBG517781.
OMA	IGWLTEN.
PhylomeDB	P33232.
ProtClustDB	PRK11197.
Enzyme and pathway databases
BioCyc	EcoCyc:L-LACTDEHYDROGFMN-MONOMER. MetaCyc:L-LACTDEHYDROGFMN-MONOMER.
Gene expression databases
Genevestigator	P33232.
Family and domain databases
HAMAP	MF_01559. L-lact_dehydr. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012133. Alpha-hydoxy_acid_DH_FMN. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. IPR020920. L-lactate_DHase_cyt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00101.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LLDD_ECOLI

Accession

Primary (citable) accession number: P33232
Secondary accession number(s): Q2M7R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program