L-lactate dehydrogenase [cytochrome] - Escherichia coli (strain K12)

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P33232 (LLDD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase [cytochrome]
EC=1.1.2.3

Gene names

Name:	lldD
Synonyms:	lctD
Ordered Locus Names:	b3605, JW3580

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H⁺. HAMAP-Rule MF_01559
Cofactor	FMN.
Induction	By L-lactate; aerobically. HAMAP-Rule MF_01559
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-fucose catabolic process Inferred from expression pattern PubMed 4595205. Source: EcoCyc aerobic respiration Inferred from expression pattern Ref.1. Source: EcoCyc lactate oxidation Inferred from mutant phenotype Ref.1. Source: EcoCyc
Cellular_component	plasma membrane Inferred from direct assay PubMed 342518. Source: EcoCyc
Molecular_function	FMN binding Inferred from direct assay PubMed 18473. Source: EcoCyc L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: HAMAP L-lactate dehydrogenase activity Inferred from direct assay PubMed 18473. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yfgL	P77774	1	EBI-553210,EBI-907297

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 396

396

L-lactate dehydrogenase [cytochrome] HAMAP-Rule MF_01559

PRO_0000206335

Regions

Domain

1 – 380

380

FMN hydroxy acid dehydrogenase

Nucleotide binding

306 – 330

FMN By similarity

Sites

Active site

275

Proton acceptor By similarity

Binding site

Substrate Potential

Binding site

106

FMN By similarity

Binding site

127

FMN By similarity

Binding site

129

Substrate By similarity

Binding site

155

FMN By similarity

Binding site

164

Substrate By similarity

Binding site

251

FMN By similarity

Binding site

278

Substrate Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P33232 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: CA0D8E308713BF83
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FASTA

396

42,728

        10         20         30         40         50         60 
MIISAASDYR AAAQRILPPF LFHYMDGGAY SEYTLRRNVE DLSEVALRQR ILKNMSDLSL 

        70         80         90        100        110        120 
ETTLFNEKLS MPVALAPVGL CGMYARRGEV QAAKAADAHG IPFTLSTVSV CPIEEVAPAI 

       130        140        150        160        170        180 
KRPMWFQLYV LRDRGFMRNA LERAKAAGCS TLVFTVDMPT PGARYRDAHS GMSGPNAAMR 

       190        200        210        220        230        240 
RYLQAVTHPQ WAWDVGLNGR PHDLGNISAY LGKPTGLEDY IGWLGNNFDP SISWKDLEWI 

       250        260        270        280        290        300 
RDFWDGPMVI KGILDPEDAR DAVRFGADGI VVSNHGGRQL DGVLSSARAL PAIADAVKGD 

       310        320        330        340        350        360 
IAILADSGIR NGLDVVRMIA LGADTVLLGR AFLYALATAG QAGVANLLNL IEKEMKVAMT 

       370        380        390 
LTGAKSISEI TQDSLVQGLG KELPAALAPM AKGNAA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three overlapping lct genes involved in L-lactate utilization by Escherichia coli." Dong J.M., Taylor J.S., Latour D.J., Iuchi S., Lin E.C.C. J. Bacteriol. 175:6671-6678(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L13970 Unassigned DNA. Translation: AAA03585.1. U00039 Genomic DNA. Translation: AAB18582.1. U00096 Genomic DNA. Translation: AAC76629.1. AP009048 Genomic DNA. Translation: BAE77687.1.
PIR	C49904.
RefSeq	NP_418062.1. NC_000913.2. YP_491828.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P33232.
SMR	P33232. Positions 8-375.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10108N.
IntAct	P33232. 14 interactions.
STRING	511145.b3605.
Proteomic databases
PaxDb	P33232.
PRIDE	P33232.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76629; AAC76629; b3605. BAE77687; BAE77687; BAE77687.
GeneID	12933567. 948121.
KEGG	ecj:Y75_p3569. eco:b3605.
PATRIC	32122695. VBIEscCol129921_3724.
Organism-specific databases
EchoBASE	EB1906.
EcoGene	EG11963. lldD.
Phylogenomic databases
eggNOG	COG1304.
HOGENOM	HOG000217464.
KO	K00101.
OMA	TYRGNPT.
ProtClustDB	PRK11197.
Enzyme and pathway databases
BioCyc	EcoCyc:L-LACTDEHYDROGFMN-MONOMER. ECOL316407:JW3580-MONOMER. MetaCyc:L-LACTDEHYDROGFMN-MONOMER.
Gene expression databases
Genevestigator	P33232.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01559. L-lact_dehydr.
InterPro	IPR013785. Aldolase_TIM. IPR012133. Alpha-hydoxy_acid_DH_FMN. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. IPR020920. L-lactate_DHase_cyt. [Graphical view]
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LLDD_ECOLI

Accession

Primary (citable) accession number: P33232
Secondary accession number(s): Q2M7R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents