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Reviewed, UniProtKB/Swiss-Prot P33232 (LLDD_ECOLI)

Last modified July 13, 2010. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
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Names and originHide

Protein namesRecommended name:
L-lactate dehydrogenase [cytochrome]
EC=1.1.2.3
Gene names
Name:lldD
Synonyms:lctD
Ordered Locus Names:b3605, JW3580
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia
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Protein attributesHide

Sequence length396 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.
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General annotation (Comments)Hide

Catalytic activity

(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+. HAMAP MF_01559

Cofactor

FMN. HAMAP MF_01559

Induction

By L-lactate; aerobically. HAMAP MF_01559

Sequence similarities

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.

Contains 1 FMN hydroxy acid dehydrogenase domain.

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Binary interactionsHide

With

Entry

#Exp.

IntAct

Notes

yfgLP777741EBI-553210,EBI-907297
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Sequence annotation (Features)Hide

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396L-lactate dehydrogenase [cytochrome] HAMAP MF_01559
PRO_0000206335

Regions

Domain1 – 380380FMN hydroxy acid dehydrogenase
Nucleotide binding306 – 33025FMN By similarity

Sites

Active site2751Proton acceptor By similarity
Binding site241Substrate Potential
Binding site1061FMN By similarity
Binding site1271FMN By similarity
Binding site1291Substrate By similarity
Binding site1551FMN By similarity
Binding site1641Substrate By similarity
Binding site2511FMN By similarity
Binding site2781Substrate Potential
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SequencesHide

Sequence LengthMass (Da)Tools
P33232-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: CA0D8E308713BF83

FASTA39642,728
        10         20         30         40         50         60 
MIISAASDYR AAAQRILPPF LFHYMDGGAY SEYTLRRNVE DLSEVALRQR ILKNMSDLSL 

        70         80         90        100        110        120 
ETTLFNEKLS MPVALAPVGL CGMYARRGEV QAAKAADAHG IPFTLSTVSV CPIEEVAPAI 

       130        140        150        160        170        180 
KRPMWFQLYV LRDRGFMRNA LERAKAAGCS TLVFTVDMPT PGARYRDAHS GMSGPNAAMR 

       190        200        210        220        230        240 
RYLQAVTHPQ WAWDVGLNGR PHDLGNISAY LGKPTGLEDY IGWLGNNFDP SISWKDLEWI 

       250        260        270        280        290        300 
RDFWDGPMVI KGILDPEDAR DAVRFGADGI VVSNHGGRQL DGVLSSARAL PAIADAVKGD 

       310        320        330        340        350        360 
IAILADSGIR NGLDVVRMIA LGADTVLLGR AFLYALATAG QAGVANLLNL IEKEMKVAMT 

       370        380        390 
LTGAKSISEI TQDSLVQGLG KELPAALAPM AKGNAA

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ReferencesHide

« Hide 'large scale' references
[1]"Three overlapping lct genes involved in L-lactate utilization by Escherichia coli."
Dong J.M., Taylor J.S., Latour D.J., Iuchi S., Lin E.C.C.
J. Bacteriol. 175:6671-6678(1993) [PubMed: 8407843] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
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Cross-referencesHide

Sequence databases

EMBL
GenBank
DDBJ		L13970 Unassigned DNA. Translation: AAA03585.1.
U00039 Genomic DNA. Translation: AAB18582.1.
U00096 Genomic DNA. Translation: AAC76629.1.
AP009048 Genomic DNA. Translation: BAE77687.1.
PIRC49904.
RefSeqAP_004186.1.
NP_418062.1.

3D structure databases

SMRP33232. Positions 1-377.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10108N.
IntActP33232. 14 interactions.
STRINGP33232.

Genome annotation databases

EnsemblBacteriaEBESCT00000000928; EBESCP00000000928; EBESCG00000000766.
EBESCT00000000929; EBESCP00000000929; EBESCG00000000766.
EBESCT00000014840; EBESCP00000014131; EBESCG00000013900.
GeneID948121.
GenomeReviewsGene locus JW3580 in contig AP009048_GR.
Gene locus b3605 in contig U00096_GR.
KEGGecj:JW3580.
eco:b3605.

Organism-specific databases

EchoBASEEB1906.
EcoGeneEG11963. lldD.
CMRSearch...

Phylogenomic databases

eggNOGCOG1304.
HOGENOMHBG517781.
OMAWIREFWE.
ProtClustDBPRK11197.

Enzyme and pathway databases

BioCycEcoCyc:L-LACTDEHYDROGFMN-MONOMER.
ECOL168927:B3605-MONOMER.
MetaCyc:L-LACTDEHYDROGFMN-MONOMER.

Gene expression databases

GenevestigatorP33232.

Family and domain databases

HAMAPMF_01559. L-lact_dehydr.
[Tree]
InterProIPR012133. a-Hydoxy_acid_DH_FMN.
IPR013785. Aldolase_TIM.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
IPR020920. L-lactate_DHase_cyt.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PfamPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]
ProtoNetSearch...
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Entry informationHide

Entry nameLLDD_ECOLI
AccessionPrimary (citable) accession number: P33232
Secondary accession number(s): Q2M7R9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 13, 2010
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)
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Relevant documentsHide

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents