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Protein

Double-strand break reduction protein

Gene

rcbA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Helps to maintain the integrity of the chromosome by lowering the steady-state level of double strand breaks (PubMed:22343303). This region of DNA acts as an antitoxin to toxin RalR, a DNase, but it seems to be sRNA RalA that has the antitoxin activity and not this putative protein. Therefore the identity of this as a protein-coding gene has been cast into doubt (PubMed:24748661).2 Publications

GO - Biological processi

  • DNA metabolic process Source: EcoCyc
  • response to antibiotic Source: UniProtKB-KW

Keywordsi

Biological processAntibiotic resistance

Enzyme and pathway databases

BioCyciEcoCyc:EG11901-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Double-strand break reduction protein1 Publication
Gene namesi
Name:rcbA1 Publication
Synonyms:ydaC
Ordered Locus Names:b1347, JW1341
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG11901 ydaC

Pathology & Biotechi

Disruption phenotypei

Mutants show increased numbers of double-strand breaks (PubMed:22343303). The single ralR and double ralR-ralA mutant have increased sensitivity to the antibiotic fosfomycin.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1M → T: No protein should be translated, DNA region still acts as an antitoxin to RalR. 1 Publication1
Mutagenesisi9 – 69Missing : No protein should be translated, DNA region still acts as an antitoxin to RalR. 1 PublicationAdd BLAST61

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001689011 – 69Double-strand break reduction proteinAdd BLAST69

Proteomic databases

PaxDbiP33230
PRIDEiP33230

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ubiFP757282EBI-562744,EBI-562588

Protein-protein interaction databases

BioGridi4261948, 13 interactors
DIPiDIP-11619N
IntActiP33230, 5 interactors
STRINGi316385.ECDH10B_1468

Structurei

3D structure databases

ProteinModelPortaliP33230
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG41070YT Bacteria
ENOG410YWP5 LUCA
HOGENOMiHOG000120153
KOiK19780
OMAiWTRYSKT

Family and domain databases

InterProiView protein in InterPro
IPR009572 DUF1187
PfamiView protein in Pfam
PF06688 DUF1187, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD055514 DUF1187, 1 hit

Sequencei

Sequence statusi: Complete.

P33230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKITATIEK EGGTPTNWTR YSKSKLTKSE CEKMLSGKKE AGVSREQKVK
60
LINFNCEKLQ SSRIALYSN
Length:69
Mass (Da):7,863
Last modified:November 1, 1997 - v2
Checksum:iD65D6454A7FBF4A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74429.1
AP009048 Genomic DNA Translation: BAA14947.1
L23927 Unassigned DNA Translation: AAA16181.1
PIRiF64884
RefSeqiNP_415863.1, NC_000913.3
WP_000276809.1, NZ_CP014272.1

Genome annotation databases

EnsemblBacteriaiAAC74429; AAC74429; b1347
BAA14947; BAA14947; BAA14947
GeneIDi947504
KEGGiecj:JW1341
eco:b1347
PATRICifig|1411691.4.peg.929

Entry informationi

Entry nameiRCBA_ECOLI
AccessioniPrimary (citable) accession number: P33230
Secondary accession number(s): P77683
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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