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Protein

Cytochrome c-type protein TorC

Gene

torC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase TorA. Acts by transferring electrons from the membranous menaquinones to TorA. This transfer probably involves an electron transfer pathway from menaquinones to the N-terminal domain of TorC, then from the N-terminus to the C-terminus, and finally to TorA. TorC apocytochrome negatively autoregulates the torCAD operon probably by inhibiting the TorS kinase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48Heme 1 (covalent)By similarity1
Binding sitei51Heme 1 (covalent)By similarity1
Metal bindingi52Iron (heme 1 axial ligand)By similarity1
Binding sitei77Heme 2 (covalent)By similarity1
Binding sitei80Heme 2 (covalent)By similarity1
Metal bindingi81Iron (heme 2 axial ligand)By similarity1
Binding sitei138Heme 3 (covalent)By similarity1
Binding sitei141Heme 3 (covalent)By similarity1
Metal bindingi142Iron (heme 3 axial ligand)By similarity1
Binding sitei170Heme 4 (covalent)By similarity1
Binding sitei173Heme 4 (covalent)By similarity1
Metal bindingi174Iron (heme 4 axial ligand)By similarity1
Binding sitei329Heme 5 (covalent)By similarity1
Binding sitei332Heme 5 (covalent)By similarity1
Metal bindingi333Iron (heme 5 axial ligand)By similarity1

GO - Molecular functioni

  • electron carrier activity Source: EcoCyc
  • heme binding Source: EcoCyc
  • iron ion binding Source: InterPro

GO - Biological processi

  • aerobic respiration Source: EcoCyc
  • anaerobic respiration Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11815-MONOMER.
ECOL316407:JW0981-MONOMER.
MetaCyc:EG11815-MONOMER.

Protein family/group databases

TCDBi5.A.3.4.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c-type protein TorC
Gene namesi
Name:torC
Ordered Locus Names:b0996, JW0981
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11815. torC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Topological domaini38 – 390PeriplasmicSequence analysisAdd BLAST353

GO - Cellular componenti

  • Gram-negative-bacterium-type cell wall Source: InterPro
  • integral component of membrane Source: EcoCyc
  • oxidoreductase complex Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi329C → S: Decrease in expression of the torCAD operon. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001084271 – 390Cytochrome c-type protein TorCAdd BLAST390

Post-translational modificationi

Binds 5 heme groups per subunit.By similarity

Proteomic databases

PaxDbiP33226.
PRIDEiP33226.

Interactioni

Subunit structurei

The N-terminal domain interacts with TorA. The immature C-terminal domain can bind to the N-terminal detector region of TorS.

Binary interactionsi

WithEntry#Exp.IntActNotes
torAP332253EBI-553710,EBI-557008

Protein-protein interaction databases

BioGridi4261495. 10 interactors.
DIPiDIP-11014N.
IntActiP33226. 2 interactors.
MINTiMINT-1285841.
STRINGi511145.b0996.

Structurei

3D structure databases

ProteinModelPortaliP33226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TorC/TorY family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CUW. Bacteria.
COG3005. LUCA.
HOGENOMiHOG000284378.
KOiK03532.
OMAiMISRIWK.

Family and domain databases

InterProiIPR009154. Membr-bd_4haem_cyt_TorC.
IPR011031. Multihaem_cyt.
IPR005126. NapC/NirT_cyt_c_N.
[Graphical view]
PfamiPF03264. Cytochrom_NNT. 1 hit.
[Graphical view]
PIRSFiPIRSF000014. 4_hem_cytch_TorC. 1 hit.
SUPFAMiSSF48695. SSF48695. 1 hit.
TIGRFAMsiTIGR02162. torC. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33226-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKLWNALRR PSARWSVLAL VAIGIVIGIA LIVLPHVGIK VTSTTEFCVS
60 70 80 90 100
CHSMQPVYEE YKQSVHFQNA SGVRAECHDC HIPPDIPGMV KRKLEASNDI
110 120 130 140 150
YQTFIAHSID TPEKFEAKRA ELAEREWARM KENNSATCRS CHNYDAMDHA
160 170 180 190 200
KQHPEAARQM KVAAKDNQSC IDCHKGIAHQ LPDMSSGFRK QFDELRASAN
210 220 230 240 250
DSGDTLYSID IKPIYAAKGD KEASGSLLPA SEVKVLKRDG DWLQIEITGW
260 270 280 290 300
TESAGRQRVL TQFPGKRIFV ASIRGDVQQQ VKTLEKTTVA DTNTEWSKLQ
310 320 330 340 350
ATAWMKKGDM VNDIKPIWAY ADSLYNGTCN QCHGAPEIAH FDANGWIGTL
360 370 380 390
NGMIGFTSLD KREERTLLKY LQMNASDTAG KAHGDKKEEK
Length:390
Mass (Da):43,607
Last modified:November 1, 1997 - v3
Checksum:i3035F71D4B32F22B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194 – 195EL → DV in CAA52094 (PubMed:8022286).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73888 Genomic DNA. Translation: CAA52094.1.
U00096 Genomic DNA. Translation: AAC74081.1.
AP009048 Genomic DNA. Translation: BAA36138.1.
PIRiB64841. S34221.
RefSeqiNP_415516.1. NC_000913.3.
WP_001230242.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74081; AAC74081; b0996.
BAA36138; BAA36138; BAA36138.
GeneIDi946252.
KEGGiecj:JW0981.
eco:b0996.
PATRICi32117217. VBIEscCol129921_1032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73888 Genomic DNA. Translation: CAA52094.1.
U00096 Genomic DNA. Translation: AAC74081.1.
AP009048 Genomic DNA. Translation: BAA36138.1.
PIRiB64841. S34221.
RefSeqiNP_415516.1. NC_000913.3.
WP_001230242.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261495. 10 interactors.
DIPiDIP-11014N.
IntActiP33226. 2 interactors.
MINTiMINT-1285841.
STRINGi511145.b0996.

Protein family/group databases

TCDBi5.A.3.4.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Proteomic databases

PaxDbiP33226.
PRIDEiP33226.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74081; AAC74081; b0996.
BAA36138; BAA36138; BAA36138.
GeneIDi946252.
KEGGiecj:JW0981.
eco:b0996.
PATRICi32117217. VBIEscCol129921_1032.

Organism-specific databases

EchoBASEiEB1762.
EcoGeneiEG11815. torC.

Phylogenomic databases

eggNOGiENOG4105CUW. Bacteria.
COG3005. LUCA.
HOGENOMiHOG000284378.
KOiK03532.
OMAiMISRIWK.

Enzyme and pathway databases

BioCyciEcoCyc:EG11815-MONOMER.
ECOL316407:JW0981-MONOMER.
MetaCyc:EG11815-MONOMER.

Miscellaneous databases

PROiP33226.

Family and domain databases

InterProiIPR009154. Membr-bd_4haem_cyt_TorC.
IPR011031. Multihaem_cyt.
IPR005126. NapC/NirT_cyt_c_N.
[Graphical view]
PfamiPF03264. Cytochrom_NNT. 1 hit.
[Graphical view]
PIRSFiPIRSF000014. 4_hem_cytch_TorC. 1 hit.
SUPFAMiSSF48695. SSF48695. 1 hit.
TIGRFAMsiTIGR02162. torC. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTORC_ECOLI
AccessioniPrimary (citable) accession number: P33226
Secondary accession number(s): P77446
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 148 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.