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Protein

Cytochrome c-type protein TorC

Gene

torC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase TorA. Acts by transferring electrons from the membranous menaquinones to TorA. This transfer probably involves an electron transfer pathway from menaquinones to the N-terminal domain of TorC, then from the N-terminus to the C-terminus, and finally to TorA. TorC apocytochrome negatively autoregulates the torCAD operon probably by inhibiting the TorS kinase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481Heme 1 (covalent)By similarity
Binding sitei51 – 511Heme 1 (covalent)By similarity
Metal bindingi52 – 521Iron (heme 1 axial ligand)By similarity
Binding sitei77 – 771Heme 2 (covalent)By similarity
Binding sitei80 – 801Heme 2 (covalent)By similarity
Metal bindingi81 – 811Iron (heme 2 axial ligand)By similarity
Binding sitei138 – 1381Heme 3 (covalent)By similarity
Binding sitei141 – 1411Heme 3 (covalent)By similarity
Metal bindingi142 – 1421Iron (heme 3 axial ligand)By similarity
Binding sitei170 – 1701Heme 4 (covalent)By similarity
Binding sitei173 – 1731Heme 4 (covalent)By similarity
Metal bindingi174 – 1741Iron (heme 4 axial ligand)By similarity
Binding sitei329 – 3291Heme 5 (covalent)By similarity
Binding sitei332 – 3321Heme 5 (covalent)By similarity
Metal bindingi333 – 3331Iron (heme 5 axial ligand)By similarity

GO - Molecular functioni

  1. electron carrier activity Source: EcoCyc
  2. heme binding Source: InterPro
  3. iron ion binding Source: EcoCyc

GO - Biological processi

  1. aerobic respiration Source: EcoCyc
  2. anaerobic respiration Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11815-MONOMER.
ECOL316407:JW0981-MONOMER.
MetaCyc:EG11815-MONOMER.

Protein family/group databases

TCDBi5.A.3.4.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c-type protein TorC
Gene namesi
Name:torC
Ordered Locus Names:b0996, JW0981
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11815. torC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei17 – 3721HelicalSequence AnalysisAdd
BLAST
Topological domaini38 – 390353PeriplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Gram-negative-bacterium-type cell wall Source: InterPro
  2. integral component of membrane Source: EcoCyc
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi329 – 3291C → S: Decrease in expression of the torCAD operon. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Cytochrome c-type protein TorCPRO_0000108427Add
BLAST

Post-translational modificationi

Binds 5 heme groups per subunit.By similarity

Proteomic databases

PRIDEiP33226.

Expressioni

Gene expression databases

GenevestigatoriP33226.

Interactioni

Subunit structurei

The N-terminal domain interacts with TorA. The immature C-terminal domain can bind to the N-terminal detector region of TorS.

Protein-protein interaction databases

DIPiDIP-11014N.
IntActiP33226. 1 interaction.
MINTiMINT-1285841.
STRINGi511145.b0996.

Structurei

3D structure databases

ProteinModelPortaliP33226.
SMRiP33226. Positions 20-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TorC/TorY family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3005.
HOGENOMiHOG000284378.
KOiK03532.
OMAiDAMDHAR.
OrthoDBiEOG6RNQDS.

Family and domain databases

InterProiIPR009154. Membr-bd_4haem_cyt_TorC.
IPR011031. Multihaem_cyt.
IPR005126. NapC/NirT_cyt_c_N.
[Graphical view]
PfamiPF03264. Cytochrom_NNT. 1 hit.
[Graphical view]
PIRSFiPIRSF000014. 4_hem_cytch_TorC. 1 hit.
TIGRFAMsiTIGR02162. torC. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33226-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKLWNALRR PSARWSVLAL VAIGIVIGIA LIVLPHVGIK VTSTTEFCVS
60 70 80 90 100
CHSMQPVYEE YKQSVHFQNA SGVRAECHDC HIPPDIPGMV KRKLEASNDI
110 120 130 140 150
YQTFIAHSID TPEKFEAKRA ELAEREWARM KENNSATCRS CHNYDAMDHA
160 170 180 190 200
KQHPEAARQM KVAAKDNQSC IDCHKGIAHQ LPDMSSGFRK QFDELRASAN
210 220 230 240 250
DSGDTLYSID IKPIYAAKGD KEASGSLLPA SEVKVLKRDG DWLQIEITGW
260 270 280 290 300
TESAGRQRVL TQFPGKRIFV ASIRGDVQQQ VKTLEKTTVA DTNTEWSKLQ
310 320 330 340 350
ATAWMKKGDM VNDIKPIWAY ADSLYNGTCN QCHGAPEIAH FDANGWIGTL
360 370 380 390
NGMIGFTSLD KREERTLLKY LQMNASDTAG KAHGDKKEEK
Length:390
Mass (Da):43,607
Last modified:November 1, 1997 - v3
Checksum:i3035F71D4B32F22B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1952EL → DV in CAA52094 (PubMed:8022286).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73888 Genomic DNA. Translation: CAA52094.1.
U00096 Genomic DNA. Translation: AAC74081.1.
AP009048 Genomic DNA. Translation: BAA36138.1.
PIRiB64841. S34221.
RefSeqiNP_415516.1. NC_000913.3.
YP_489269.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74081; AAC74081; b0996.
BAA36138; BAA36138; BAA36138.
GeneIDi12932501.
946252.
KEGGiecj:Y75_p0969.
eco:b0996.
PATRICi32117217. VBIEscCol129921_1032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73888 Genomic DNA. Translation: CAA52094.1.
U00096 Genomic DNA. Translation: AAC74081.1.
AP009048 Genomic DNA. Translation: BAA36138.1.
PIRiB64841. S34221.
RefSeqiNP_415516.1. NC_000913.3.
YP_489269.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP33226.
SMRiP33226. Positions 20-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-11014N.
IntActiP33226. 1 interaction.
MINTiMINT-1285841.
STRINGi511145.b0996.

Protein family/group databases

TCDBi5.A.3.4.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Proteomic databases

PRIDEiP33226.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74081; AAC74081; b0996.
BAA36138; BAA36138; BAA36138.
GeneIDi12932501.
946252.
KEGGiecj:Y75_p0969.
eco:b0996.
PATRICi32117217. VBIEscCol129921_1032.

Organism-specific databases

EchoBASEiEB1762.
EcoGeneiEG11815. torC.

Phylogenomic databases

eggNOGiCOG3005.
HOGENOMiHOG000284378.
KOiK03532.
OMAiDAMDHAR.
OrthoDBiEOG6RNQDS.

Enzyme and pathway databases

BioCyciEcoCyc:EG11815-MONOMER.
ECOL316407:JW0981-MONOMER.
MetaCyc:EG11815-MONOMER.

Miscellaneous databases

PROiP33226.

Gene expression databases

GenevestigatoriP33226.

Family and domain databases

InterProiIPR009154. Membr-bd_4haem_cyt_TorC.
IPR011031. Multihaem_cyt.
IPR005126. NapC/NirT_cyt_c_N.
[Graphical view]
PfamiPF03264. Cytochrom_NNT. 1 hit.
[Graphical view]
PIRSFiPIRSF000014. 4_hem_cytch_TorC. 1 hit.
TIGRFAMsiTIGR02162. torC. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "TMAO anaerobic respiration in Escherichia coli: involvement of the tor operon."
    Mejean V., Iobbi-Nivol C., Lepelletier M., Giordano G., Chippaux M., Pascal M.-C.
    Mol. Microbiol. 11:1169-1179(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Pascal M.-C.
    Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 73-76.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "A reassessment of the range of c-type cytochromes synthesized by Escherichia coli K-12."
    Iobbi-Nivol C., Crooke H., Griffiths L., Grov J., Hussain H., Pommier J., Mejean V., Cole J.A.
    FEMS Microbiol. Lett. 119:89-94(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION AS A CYTOCHROME C.
  7. "TorC apocytochrome negatively autoregulates the trimethylamine N-oxide (TMAO) reductase operon in Escherichia coli."
    Ansaldi M., Bordi C., Lepelletier M., Mejean V.
    Mol. Microbiol. 33:284-295(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  8. "Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase in Escherichia coli."
    Gon S., Giudici-Orticoni M.-T., Mejean V., Iobbi-Nivol C.
    J. Biol. Chem. 276:11545-11551(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  9. "An unsuspected autoregulatory pathway involving apocytochrome TorC and sensor TorS in Escherichia coli."
    Gon S., Jourlin-Castelli C., Theraulaz L., Mejean V.
    Proc. Natl. Acad. Sci. U.S.A. 98:11615-11620(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-329.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiTORC_ECOLI
AccessioniPrimary (citable) accession number: P33226
Secondary accession number(s): P77446
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.