Cytochrome c-type protein TorC - Escherichia coli (strain K12)

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P33226 (TORC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 123. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome c-type protein TorC

Gene names

Name:	torC
Ordered Locus Names:	b0996, JW0981

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase TorA. Acts by transferring electrons from the membranous menaquinones to TorA. This transfer probably involves an electron transfer pathway from menaquinones to the N-terminal domain of TorC, then from the N-terminus to the C-terminus, and finally to TorA. TorC apocytochrome negatively autoregulates the torCAD operon probably by inhibiting the TorS kinase activity.
Subunit structure	The N-terminal domain interacts with TorA. The immature C-terminal domain can bind to the N-terminal detector region of TorS.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein.
Post-translational modification	Binds 5 heme groups per subunit By similarity.
Sequence similarities	Belongs to the TorC/TorY family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aerobic respiration Inferred from expression pattern PubMed 17850256. Source: EcoCyc anaerobic respiration Inferred from expression pattern PubMed 17850256. Source: EcoCyc electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from direct assay Ref.1. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	electron carrier activity Inferred from direct assay Ref.8. Source: EcoCyc heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from sequence or structural similarity Ref.9. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 390

390

Cytochrome c-type protein TorC

PRO_0000108427

Regions

Topological domain

1 – 16

Cytoplasmic Potential

Transmembrane

17 – 37

Helical; Potential

Topological domain

38 – 390

353

Periplasmic Potential

Sites

Metal binding

Iron (heme 1 axial ligand) By similarity

Metal binding

Iron (heme 2 axial ligand) By similarity

Metal binding

142

Iron (heme 3 axial ligand) By similarity

Metal binding

174

Iron (heme 4 axial ligand) By similarity

Metal binding

333

Iron (heme 5 axial ligand) By similarity

Binding site

Heme 1 (covalent) By similarity

Binding site

Heme 1 (covalent) By similarity

Binding site

Heme 2 (covalent) By similarity

Binding site

Heme 2 (covalent) By similarity

Binding site

138

Heme 3 (covalent) By similarity

Binding site

141

Heme 3 (covalent) By similarity

Binding site

170

Heme 4 (covalent) By similarity

Binding site

173

Heme 4 (covalent) By similarity

Binding site

329

Heme 5 (covalent) By similarity

Binding site

332

Heme 5 (covalent) By similarity

Experimental info

Mutagenesis

329

C → S: Decrease in expression of the torCAD operon. Ref.9

Sequence conflict

194 – 195

EL → DV in CAA52094. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P33226 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 3035F71D4B32F22B
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FASTA

390

43,607

        10         20         30         40         50         60 
MRKLWNALRR PSARWSVLAL VAIGIVIGIA LIVLPHVGIK VTSTTEFCVS CHSMQPVYEE 

        70         80         90        100        110        120 
YKQSVHFQNA SGVRAECHDC HIPPDIPGMV KRKLEASNDI YQTFIAHSID TPEKFEAKRA 

       130        140        150        160        170        180 
ELAEREWARM KENNSATCRS CHNYDAMDHA KQHPEAARQM KVAAKDNQSC IDCHKGIAHQ 

       190        200        210        220        230        240 
LPDMSSGFRK QFDELRASAN DSGDTLYSID IKPIYAAKGD KEASGSLLPA SEVKVLKRDG 

       250        260        270        280        290        300 
DWLQIEITGW TESAGRQRVL TQFPGKRIFV ASIRGDVQQQ VKTLEKTTVA DTNTEWSKLQ 

       310        320        330        340        350        360 
ATAWMKKGDM VNDIKPIWAY ADSLYNGTCN QCHGAPEIAH FDANGWIGTL NGMIGFTSLD 

       370        380        390 
KREERTLLKY LQMNASDTAG KAHGDKKEEK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TMAO anaerobic respiration in Escherichia coli: involvement of the tor operon." Mejean V., Iobbi-Nivol C., Lepelletier M., Giordano G., Chippaux M., Pascal M.-C. Mol. Microbiol. 11:1169-1179(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	Pascal M.-C. Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 73-76.
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"A reassessment of the range of c-type cytochromes synthesized by Escherichia coli K-12." Iobbi-Nivol C., Crooke H., Griffiths L., Grov J., Hussain H., Pommier J., Mejean V., Cole J.A. FEMS Microbiol. Lett. 119:89-94(1994) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION AS A CYTOCHROME C.
[7]	"TorC apocytochrome negatively autoregulates the trimethylamine N-oxide (TMAO) reductase operon in Escherichia coli." Ansaldi M., Bordi C., Lepelletier M., Mejean V. Mol. Microbiol. 33:284-295(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[8]	"Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase in Escherichia coli." Gon S., Giudici-Orticoni M.-T., Mejean V., Iobbi-Nivol C. J. Biol. Chem. 276:11545-11551(2001) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[9]	"An unsuspected autoregulatory pathway involving apocytochrome TorC and sensor TorS in Escherichia coli." Gon S., Jourlin-Castelli C., Theraulaz L., Mejean V. Proc. Natl. Acad. Sci. U.S.A. 98:11615-11620(2001) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-329. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X73888 Genomic DNA. Translation: CAA52094.1. U00096 Genomic DNA. Translation: AAC74081.1. AP009048 Genomic DNA. Translation: BAA36138.1.
PIR	S34221. B64841.
RefSeq	NP_415516.1. NC_000913.2. YP_489269.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P33226.
SMR	P33226. Positions 20-179, 323-377.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11014N.
IntAct	P33226. 1 interaction.
MINT	MINT-1285841.
STRING	511145.b0996.
Protein family/group databases
TCDB	5.A.3.4.1. prokaryotic molybdopterin-containing oxidoreductase (PMO) family.
Proteomic databases
PRIDE	P33226.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74081; AAC74081; b0996. BAA36138; BAA36138; BAA36138.
GeneID	12932501. 946252.
KEGG	ecj:Y75_p0969. eco:b0996.
PATRIC	32117217. VBIEscCol129921_1032.
Organism-specific databases
EchoBASE	EB1762.
EcoGene	EG11815. torC.
Phylogenomic databases
eggNOG	COG3005.
HOGENOM	HOG000284378.
KO	K03532.
OMA	DAMDHAR.
ProtClustDB	PRK15032.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11815-MONOMER. ECOL316407:JW0981-MONOMER.
Gene expression databases
Genevestigator	P33226.
Family and domain databases
InterPro	IPR009154. Membr-bd_4haem_cyt_TorC. IPR011031. Multihaem_cyt. IPR005126. NapC/NirT_cyt_c_N. [Graphical view]
Pfam	PF03264. Cytochrom_NNT. 1 hit. [Graphical view]
PIRSF	PIRSF000014. 4_hem_cytch_TorC. 1 hit.
TIGRFAMs	TIGR02162. torC. 1 hit.
PROSITE	PS51008. MULTIHEME_CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TORC_ECOLI

Accession

Primary (citable) accession number: P33226
Secondary accession number(s): P77446

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 123 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents