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P33226

- TORC_ECOLI

UniProt

P33226 - TORC_ECOLI

Protein

Cytochrome c-type protein TorC

Gene

torC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 3 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase TorA. Acts by transferring electrons from the membranous menaquinones to TorA. This transfer probably involves an electron transfer pathway from menaquinones to the N-terminal domain of TorC, then from the N-terminus to the C-terminus, and finally to TorA. TorC apocytochrome negatively autoregulates the torCAD operon probably by inhibiting the TorS kinase activity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei48 – 481Heme 1 (covalent)By similarity
    Binding sitei51 – 511Heme 1 (covalent)By similarity
    Metal bindingi52 – 521Iron (heme 1 axial ligand)By similarity
    Binding sitei77 – 771Heme 2 (covalent)By similarity
    Binding sitei80 – 801Heme 2 (covalent)By similarity
    Metal bindingi81 – 811Iron (heme 2 axial ligand)By similarity
    Binding sitei138 – 1381Heme 3 (covalent)By similarity
    Binding sitei141 – 1411Heme 3 (covalent)By similarity
    Metal bindingi142 – 1421Iron (heme 3 axial ligand)By similarity
    Binding sitei170 – 1701Heme 4 (covalent)By similarity
    Binding sitei173 – 1731Heme 4 (covalent)By similarity
    Metal bindingi174 – 1741Iron (heme 4 axial ligand)By similarity
    Binding sitei329 – 3291Heme 5 (covalent)By similarity
    Binding sitei332 – 3321Heme 5 (covalent)By similarity
    Metal bindingi333 – 3331Iron (heme 5 axial ligand)By similarity

    GO - Molecular functioni

    1. electron carrier activity Source: EcoCyc
    2. heme binding Source: InterPro
    3. iron ion binding Source: EcoCyc
    4. protein binding Source: EcoliWiki

    GO - Biological processi

    1. aerobic respiration Source: EcoCyc
    2. anaerobic respiration Source: EcoCyc

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11815-MONOMER.
    ECOL316407:JW0981-MONOMER.
    MetaCyc:EG11815-MONOMER.

    Protein family/group databases

    TCDBi5.A.3.4.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c-type protein TorC
    Gene namesi
    Name:torC
    Ordered Locus Names:b0996, JW0981
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11815. torC.

    Subcellular locationi

    GO - Cellular componenti

    1. Gram-negative-bacterium-type cell wall Source: InterPro
    2. integral component of membrane Source: EcoCyc
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi329 – 3291C → S: Decrease in expression of the torCAD operon. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Cytochrome c-type protein TorCPRO_0000108427Add
    BLAST

    Post-translational modificationi

    Binds 5 heme groups per subunit.By similarity

    Proteomic databases

    PRIDEiP33226.

    Expressioni

    Gene expression databases

    GenevestigatoriP33226.

    Interactioni

    Subunit structurei

    The N-terminal domain interacts with TorA. The immature C-terminal domain can bind to the N-terminal detector region of TorS.

    Protein-protein interaction databases

    DIPiDIP-11014N.
    IntActiP33226. 1 interaction.
    MINTiMINT-1285841.
    STRINGi511145.b0996.

    Structurei

    3D structure databases

    ProteinModelPortaliP33226.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini38 – 390353PeriplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei17 – 3721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TorC/TorY family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG3005.
    HOGENOMiHOG000284378.
    KOiK03532.
    OMAiDAMDHAR.
    OrthoDBiEOG6RNQDS.

    Family and domain databases

    InterProiIPR009154. Membr-bd_4haem_cyt_TorC.
    IPR011031. Multihaem_cyt.
    IPR005126. NapC/NirT_cyt_c_N.
    [Graphical view]
    PfamiPF03264. Cytochrom_NNT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000014. 4_hem_cytch_TorC. 1 hit.
    TIGRFAMsiTIGR02162. torC. 1 hit.
    PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33226-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKLWNALRR PSARWSVLAL VAIGIVIGIA LIVLPHVGIK VTSTTEFCVS    50
    CHSMQPVYEE YKQSVHFQNA SGVRAECHDC HIPPDIPGMV KRKLEASNDI 100
    YQTFIAHSID TPEKFEAKRA ELAEREWARM KENNSATCRS CHNYDAMDHA 150
    KQHPEAARQM KVAAKDNQSC IDCHKGIAHQ LPDMSSGFRK QFDELRASAN 200
    DSGDTLYSID IKPIYAAKGD KEASGSLLPA SEVKVLKRDG DWLQIEITGW 250
    TESAGRQRVL TQFPGKRIFV ASIRGDVQQQ VKTLEKTTVA DTNTEWSKLQ 300
    ATAWMKKGDM VNDIKPIWAY ADSLYNGTCN QCHGAPEIAH FDANGWIGTL 350
    NGMIGFTSLD KREERTLLKY LQMNASDTAG KAHGDKKEEK 390
    Length:390
    Mass (Da):43,607
    Last modified:November 1, 1997 - v3
    Checksum:i3035F71D4B32F22B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti194 – 1952EL → DV in CAA52094. (PubMed:8022286)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73888 Genomic DNA. Translation: CAA52094.1.
    U00096 Genomic DNA. Translation: AAC74081.1.
    AP009048 Genomic DNA. Translation: BAA36138.1.
    PIRiB64841. S34221.
    RefSeqiNP_415516.1. NC_000913.3.
    YP_489269.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74081; AAC74081; b0996.
    BAA36138; BAA36138; BAA36138.
    GeneIDi12932501.
    946252.
    KEGGiecj:Y75_p0969.
    eco:b0996.
    PATRICi32117217. VBIEscCol129921_1032.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73888 Genomic DNA. Translation: CAA52094.1 .
    U00096 Genomic DNA. Translation: AAC74081.1 .
    AP009048 Genomic DNA. Translation: BAA36138.1 .
    PIRi B64841. S34221.
    RefSeqi NP_415516.1. NC_000913.3.
    YP_489269.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P33226.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-11014N.
    IntActi P33226. 1 interaction.
    MINTi MINT-1285841.
    STRINGi 511145.b0996.

    Protein family/group databases

    TCDBi 5.A.3.4.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

    Proteomic databases

    PRIDEi P33226.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74081 ; AAC74081 ; b0996 .
    BAA36138 ; BAA36138 ; BAA36138 .
    GeneIDi 12932501.
    946252.
    KEGGi ecj:Y75_p0969.
    eco:b0996.
    PATRICi 32117217. VBIEscCol129921_1032.

    Organism-specific databases

    EchoBASEi EB1762.
    EcoGenei EG11815. torC.

    Phylogenomic databases

    eggNOGi COG3005.
    HOGENOMi HOG000284378.
    KOi K03532.
    OMAi DAMDHAR.
    OrthoDBi EOG6RNQDS.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11815-MONOMER.
    ECOL316407:JW0981-MONOMER.
    MetaCyc:EG11815-MONOMER.

    Miscellaneous databases

    PROi P33226.

    Gene expression databases

    Genevestigatori P33226.

    Family and domain databases

    InterProi IPR009154. Membr-bd_4haem_cyt_TorC.
    IPR011031. Multihaem_cyt.
    IPR005126. NapC/NirT_cyt_c_N.
    [Graphical view ]
    Pfami PF03264. Cytochrom_NNT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000014. 4_hem_cytch_TorC. 1 hit.
    TIGRFAMsi TIGR02162. torC. 1 hit.
    PROSITEi PS51008. MULTIHEME_CYTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TMAO anaerobic respiration in Escherichia coli: involvement of the tor operon."
      Mejean V., Iobbi-Nivol C., Lepelletier M., Giordano G., Chippaux M., Pascal M.-C.
      Mol. Microbiol. 11:1169-1179(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Pascal M.-C.
      Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 73-76.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "A reassessment of the range of c-type cytochromes synthesized by Escherichia coli K-12."
      Iobbi-Nivol C., Crooke H., Griffiths L., Grov J., Hussain H., Pommier J., Mejean V., Cole J.A.
      FEMS Microbiol. Lett. 119:89-94(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION AS A CYTOCHROME C.
    7. "TorC apocytochrome negatively autoregulates the trimethylamine N-oxide (TMAO) reductase operon in Escherichia coli."
      Ansaldi M., Bordi C., Lepelletier M., Mejean V.
      Mol. Microbiol. 33:284-295(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    8. "Electron transfer and binding of the c-type cytochrome TorC to the trimethylamine N-oxide reductase in Escherichia coli."
      Gon S., Giudici-Orticoni M.-T., Mejean V., Iobbi-Nivol C.
      J. Biol. Chem. 276:11545-11551(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    9. "An unsuspected autoregulatory pathway involving apocytochrome TorC and sensor TorS in Escherichia coli."
      Gon S., Jourlin-Castelli C., Theraulaz L., Mejean V.
      Proc. Natl. Acad. Sci. U.S.A. 98:11615-11620(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-329.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

    Entry informationi

    Entry nameiTORC_ECOLI
    AccessioniPrimary (citable) accession number: P33226
    Secondary accession number(s): P77446
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 135 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3