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Protein

Trimethylamine-N-oxide reductase 1

Gene

torA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions.

Catalytic activityi

Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.

Cofactori

Mo-bis(molybdopterin guanine dinucleotide)By similarityNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi191 – 1911MolybdenumBy similarity

GO - Molecular functioni

  • cofactor binding Source: EcoCyc
  • electron carrier activity Source: InterPro
  • molybdenum ion binding Source: EcoCyc
  • trimethylamine-N-oxide reductase (cytochrome c) activity Source: EcoliWiki

GO - Biological processi

  • aerobic respiration Source: EcoCyc
  • anaerobic respiration Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciEcoCyc:TORA-MONOMER.
ECOL316407:JW0982-MONOMER.
MetaCyc:TORA-MONOMER.

Protein family/group databases

TCDBi5.A.3.4.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Trimethylamine-N-oxide reductase 1 (EC:1.7.2.3)
Short name:
TMAO reductase 1
Short name:
Trimethylamine oxidase 1
Gene namesi
Name:torA
Ordered Locus Names:b0997, JW0982
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11814. torA.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3939Tat-type signalPROSITE-ProRule annotation1 PublicationAdd
BLAST
Chaini40 – 848809Trimethylamine-N-oxide reductase 1PRO_0000019151Add
BLAST

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Proteomic databases

PaxDbiP33225.
PRIDEiP33225.

Interactioni

Subunit structurei

Interacts with the N-terminal domain of TorC.

Binary interactionsi

WithEntry#Exp.IntActNotes
lonP0A9M02EBI-557008,EBI-547203
torCP332263EBI-557008,EBI-553710
torDP3666224EBI-557008,EBI-6406226

Protein-protein interaction databases

BioGridi4260701. 7 interactions.
DIPiDIP-11013N.
IntActiP33225. 17 interactions.
MINTiMINT-6796194.
STRINGi511145.b0997.

Structurei

3D structure databases

ProteinModelPortaliP33225.
SMRiP33225. Positions 51-834.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QY8. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000284391.
InParanoidiP33225.
KOiK07811.
OMAiTIVVHDP.
PhylomeDBiP33225.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006658. BisC.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR006311. TAT_signal.
IPR011887. TorA.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR00509. bisC_fam. 1 hit.
TIGR02164. torA. 1 hit.
PROSITEiPS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33225-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNNDLFQAS RRRFLAQLGG LTVAGMLGPS LLTPRRATAA QAATDAVISK
60 70 80 90 100
EGILTGSHWG AIRATVKDGR FVAAKPFELD KYPSKMIAGL PDHVHNAARI
110 120 130 140 150
RYPMVRVDWL RKRHLSDTSQ RGDNRFVRVS WDEALDMFYE ELERVQKTHG
160 170 180 190 200
PSALLTASGW QSTGMFHNAS GMLAKAIALH GNSVGTGGDY STGAAQVILP
210 220 230 240 250
RVVGSMEVYE QQTSWPLVLQ NSKTIVLWGS DLLKNQQANW WCPDHDVYEY
260 270 280 290 300
YAQLKAKVAA GEIEVISIDP VVTSTHEYLG REHVKHIAVN PQTDVPLQLA
310 320 330 340 350
LAHTLYSENL YDKNFLANYC VGFEQFLPYL LGEKDGQPKD AAWAEKLTGI
360 370 380 390 400
DAETIRGLAR QMAANRTQII AGWCVQRMQH GEQWAWMIVV LAAMLGQIGL
410 420 430 440 450
PGGGFGFGWH YNGAGTPGRK GVILSGFSGS TSIPPVHDNS DYKGYSSTIP
460 470 480 490 500
IARFIDAILE PGKVINWNGK SVKLPPLKMC IFAGTNPFHR HQQINRIIEG
510 520 530 540 550
LRKLETVIAI DNQWTSTCRF ADIVLPATTQ FERNDLDQYG NHSNRGIIAM
560 570 580 590 600
KQVVPPQFEA RNDFDIFREL CRRFNREEAF TEGLDEMGWL KRIWQEGVQQ
610 620 630 640 650
GKGRGVHLPA FDDFWNNKEY VEFDHPQMFV RHQAFREDPD LEPLGTPSGL
660 670 680 690 700
IEIYSKTIAD MNYDDCQGHP MWFEKIERSH GGPGSQKYPL HLQSVHPDFR
710 720 730 740 750
LHSQLCESET LRQQYTVAGK EPVFINPQDA SARGIRNGDV VRVFNARGQV
760 770 780 790 800
LAGAVVSDRY APGVARIHEG AWYDPDKGGE PGALCKYGNP NVLTIDIGTS
810 820 830 840
QLAQATSAHT TLVEIEKYNG TVEQVTAFNG PVEMVAQCEY VPASQVKS
Length:848
Mass (Da):94,456
Last modified:November 1, 1997 - v2
Checksum:i59DDACB00B1843E7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731L → R in CAA52095 (PubMed:8022286).Curated
Sequence conflicti176 – 1761A → R in CAA52095 (PubMed:8022286).Curated
Sequence conflicti256 – 2561A → R AA sequence (PubMed:8022286).Curated
Sequence conflicti258 – 2581V → S AA sequence (PubMed:8022286).Curated
Sequence conflicti281 – 2811R → G in CAA52095 (PubMed:8022286).Curated
Sequence conflicti325 – 3251Q → E in CAA52095 (PubMed:8022286).Curated
Sequence conflicti348 – 3481T → S in CAA52095 (PubMed:8022286).Curated
Sequence conflicti503 – 5042KL → NV in CAA52095 (PubMed:8022286).Curated
Sequence conflicti713 – 7142QQ → HE in CAA52095 (PubMed:8022286).Curated
Sequence conflicti751 – 7511L → M in CAA52095 (PubMed:8022286).Curated
Sequence conflicti781 – 7811P → L in CAA52095 (PubMed:8022286).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73888 Genomic DNA. Translation: CAA52095.1.
U00096 Genomic DNA. Translation: AAC74082.1.
AP009048 Genomic DNA. Translation: BAA36139.1.
D16500 Genomic DNA. No translation available.
PIRiC64841.
RefSeqiNP_415517.1. NC_000913.3.
WP_001062091.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74082; AAC74082; b0997.
BAA36139; BAA36139; BAA36139.
GeneIDi946267.
KEGGiecj:JW0982.
eco:b0997.
PATRICi32117219. VBIEscCol129921_1033.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73888 Genomic DNA. Translation: CAA52095.1.
U00096 Genomic DNA. Translation: AAC74082.1.
AP009048 Genomic DNA. Translation: BAA36139.1.
D16500 Genomic DNA. No translation available.
PIRiC64841.
RefSeqiNP_415517.1. NC_000913.3.
WP_001062091.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP33225.
SMRiP33225. Positions 51-834.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260701. 7 interactions.
DIPiDIP-11013N.
IntActiP33225. 17 interactions.
MINTiMINT-6796194.
STRINGi511145.b0997.

Protein family/group databases

TCDBi5.A.3.4.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Proteomic databases

PaxDbiP33225.
PRIDEiP33225.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74082; AAC74082; b0997.
BAA36139; BAA36139; BAA36139.
GeneIDi946267.
KEGGiecj:JW0982.
eco:b0997.
PATRICi32117219. VBIEscCol129921_1033.

Organism-specific databases

EchoBASEiEB1761.
EcoGeneiEG11814. torA.

Phylogenomic databases

eggNOGiENOG4107QY8. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000284391.
InParanoidiP33225.
KOiK07811.
OMAiTIVVHDP.
PhylomeDBiP33225.

Enzyme and pathway databases

BioCyciEcoCyc:TORA-MONOMER.
ECOL316407:JW0982-MONOMER.
MetaCyc:TORA-MONOMER.

Miscellaneous databases

PROiP33225.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006658. BisC.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR006311. TAT_signal.
IPR011887. TorA.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR00509. bisC_fam. 1 hit.
TIGR02164. torA. 1 hit.
PROSITEiPS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTORA_ECOLI
AccessioniPrimary (citable) accession number: P33225
Secondary accession number(s): P78227
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.