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Protein

Trimethylamine-N-oxide reductase 1

Gene

torA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions.

Catalytic activityi

Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.

Cofactori

Mo-bis(molybdopterin guanine dinucleotide)By similarityNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi191MolybdenumBy similarity1

GO - Molecular functioni

  • cofactor binding Source: EcoCyc
  • electron transfer activity Source: InterPro
  • molybdenum ion binding Source: EcoCyc
  • molybdopterin cofactor binding Source: InterPro
  • trimethylamine-N-oxide reductase (cytochrome c) activity Source: EcoliWiki

GO - Biological processi

  • aerobic respiration Source: EcoCyc
  • anaerobic respiration Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, Molybdenum

Enzyme and pathway databases

BioCyciEcoCyc:TORA-MONOMER
MetaCyc:TORA-MONOMER

Protein family/group databases

TCDBi5.A.3.4.1 the prokaryotic molybdopterin-containing oxidoreductase (pmo) family

Names & Taxonomyi

Protein namesi
Recommended name:
Trimethylamine-N-oxide reductase 1 (EC:1.7.2.3)
Short name:
TMAO reductase 1
Short name:
Trimethylamine oxidase 1
Gene namesi
Name:torA
Ordered Locus Names:b0997, JW0982
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11814 torA

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 39Tat-type signalPROSITE-ProRule annotation1 PublicationAdd BLAST39
ChainiPRO_000001915140 – 848Trimethylamine-N-oxide reductase 1Add BLAST809

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Proteomic databases

PaxDbiP33225
PRIDEiP33225

Interactioni

Subunit structurei

Interacts with the N-terminal domain of TorC.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4260701, 16 interactors
DIPiDIP-11013N
IntActiP33225, 17 interactors
MINTiP33225
STRINGi316385.ECDH10B_1069

Structurei

3D structure databases

ProteinModelPortaliP33225
SMRiP33225
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QY8 Bacteria
COG0243 LUCA
HOGENOMiHOG000284391
InParanoidiP33225
KOiK07811
OMAiEKHGNPN
PhylomeDBiP33225

Family and domain databases

InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006658 BisC
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006655 Mopterin_OxRdtase_prok_CS
IPR006311 TAT_signal
IPR011887 TorA
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR00509 bisC_fam, 1 hit
TIGR02164 torA, 1 hit
PROSITEiView protein in PROSITE
PS00490 MOLYBDOPTERIN_PROK_2, 1 hit
PS00932 MOLYBDOPTERIN_PROK_3, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33225-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNNDLFQAS RRRFLAQLGG LTVAGMLGPS LLTPRRATAA QAATDAVISK
60 70 80 90 100
EGILTGSHWG AIRATVKDGR FVAAKPFELD KYPSKMIAGL PDHVHNAARI
110 120 130 140 150
RYPMVRVDWL RKRHLSDTSQ RGDNRFVRVS WDEALDMFYE ELERVQKTHG
160 170 180 190 200
PSALLTASGW QSTGMFHNAS GMLAKAIALH GNSVGTGGDY STGAAQVILP
210 220 230 240 250
RVVGSMEVYE QQTSWPLVLQ NSKTIVLWGS DLLKNQQANW WCPDHDVYEY
260 270 280 290 300
YAQLKAKVAA GEIEVISIDP VVTSTHEYLG REHVKHIAVN PQTDVPLQLA
310 320 330 340 350
LAHTLYSENL YDKNFLANYC VGFEQFLPYL LGEKDGQPKD AAWAEKLTGI
360 370 380 390 400
DAETIRGLAR QMAANRTQII AGWCVQRMQH GEQWAWMIVV LAAMLGQIGL
410 420 430 440 450
PGGGFGFGWH YNGAGTPGRK GVILSGFSGS TSIPPVHDNS DYKGYSSTIP
460 470 480 490 500
IARFIDAILE PGKVINWNGK SVKLPPLKMC IFAGTNPFHR HQQINRIIEG
510 520 530 540 550
LRKLETVIAI DNQWTSTCRF ADIVLPATTQ FERNDLDQYG NHSNRGIIAM
560 570 580 590 600
KQVVPPQFEA RNDFDIFREL CRRFNREEAF TEGLDEMGWL KRIWQEGVQQ
610 620 630 640 650
GKGRGVHLPA FDDFWNNKEY VEFDHPQMFV RHQAFREDPD LEPLGTPSGL
660 670 680 690 700
IEIYSKTIAD MNYDDCQGHP MWFEKIERSH GGPGSQKYPL HLQSVHPDFR
710 720 730 740 750
LHSQLCESET LRQQYTVAGK EPVFINPQDA SARGIRNGDV VRVFNARGQV
760 770 780 790 800
LAGAVVSDRY APGVARIHEG AWYDPDKGGE PGALCKYGNP NVLTIDIGTS
810 820 830 840
QLAQATSAHT TLVEIEKYNG TVEQVTAFNG PVEMVAQCEY VPASQVKS
Length:848
Mass (Da):94,456
Last modified:November 1, 1997 - v2
Checksum:i59DDACB00B1843E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173L → R in CAA52095 (PubMed:8022286).Curated1
Sequence conflicti176A → R in CAA52095 (PubMed:8022286).Curated1
Sequence conflicti256A → R AA sequence (PubMed:8022286).Curated1
Sequence conflicti258V → S AA sequence (PubMed:8022286).Curated1
Sequence conflicti281R → G in CAA52095 (PubMed:8022286).Curated1
Sequence conflicti325Q → E in CAA52095 (PubMed:8022286).Curated1
Sequence conflicti348T → S in CAA52095 (PubMed:8022286).Curated1
Sequence conflicti503 – 504KL → NV in CAA52095 (PubMed:8022286).Curated2
Sequence conflicti713 – 714QQ → HE in CAA52095 (PubMed:8022286).Curated2
Sequence conflicti751L → M in CAA52095 (PubMed:8022286).Curated1
Sequence conflicti781P → L in CAA52095 (PubMed:8022286).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73888 Genomic DNA Translation: CAA52095.1
U00096 Genomic DNA Translation: AAC74082.1
AP009048 Genomic DNA Translation: BAA36139.1
D16500 Genomic DNA No translation available.
PIRiC64841
RefSeqiNP_415517.1, NC_000913.3
WP_001062091.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74082; AAC74082; b0997
BAA36139; BAA36139; BAA36139
GeneIDi946267
KEGGiecj:JW0982
eco:b0997
PATRICifig|1411691.4.peg.1274

Entry informationi

Entry nameiTORA_ECOLI
AccessioniPrimary (citable) accession number: P33225
Secondary accession number(s): P78227
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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