Reviewed, UniProtKB/Swiss-Prot P33225 (TORA_ECOLI)

Names and origin

Protein names

Recommended name:
    Trimethylamine-N-oxide reductase 1
      Short name=TMAO reductase 1
      Short name=Trimethylamine oxidase 1
    EC=1.7.2.3

Gene names

Name:	torA
Ordered Locus Names:	b0997, JW0982

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	848 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions.
Catalytic activity	Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.
Cofactor	Molybdenum (molybdopterin) By similarity.
Subunit structure	Interacts with the N-terminal domain of torC.
Subcellular location	Periplasm. Ref.6
Post-translational modification	Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW trimethylamine-N-oxide reductase (cytochrome c) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	Tat-type signal Ref.1
	Chain	40 – 848	809	Trimethylamine-N-oxide reductase 1		PRO_0000019151
Experimental info
	Sequence conflict	173	1	L → R in CAA52095. Ref.1
	Sequence conflict	176	1	A → R in CAA52095. Ref.1
	Sequence conflict	256	1	A → R AA sequence Ref.1
	Sequence conflict	258	1	V → S AA sequence Ref.1
	Sequence conflict	281	1	R → G in CAA52095. Ref.1
	Sequence conflict	325	1	Q → E in CAA52095. Ref.1
	Sequence conflict	348	1	T → S in CAA52095. Ref.1
	Sequence conflict	503 – 504	2	KL → NV in CAA52095. Ref.1
	Sequence conflict	713 – 714	2	QQ → HE in CAA52095. Ref.1
	Sequence conflict	751	1	L → M in CAA52095. Ref.1
	Sequence conflict	781	1	P → L in CAA52095. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33225-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 59DDACB00B1843E7	FASTA	848	94,456
	10 20 30 40 50 60 MNNNDLFQAS RRRFLAQLGG LTVAGMLGPS LLTPRRATAA QAATDAVISK EGILTGSHWG 70 80 90 100 110 120 AIRATVKDGR FVAAKPFELD KYPSKMIAGL PDHVHNAARI RYPMVRVDWL RKRHLSDTSQ 130 140 150 160 170 180 RGDNRFVRVS WDEALDMFYE ELERVQKTHG PSALLTASGW QSTGMFHNAS GMLAKAIALH 190 200 210 220 230 240 GNSVGTGGDY STGAAQVILP RVVGSMEVYE QQTSWPLVLQ NSKTIVLWGS DLLKNQQANW 250 260 270 280 290 300 WCPDHDVYEY YAQLKAKVAA GEIEVISIDP VVTSTHEYLG REHVKHIAVN PQTDVPLQLA 310 320 330 340 350 360 LAHTLYSENL YDKNFLANYC VGFEQFLPYL LGEKDGQPKD AAWAEKLTGI DAETIRGLAR 370 380 390 400 410 420 QMAANRTQII AGWCVQRMQH GEQWAWMIVV LAAMLGQIGL PGGGFGFGWH YNGAGTPGRK 430 440 450 460 470 480 GVILSGFSGS TSIPPVHDNS DYKGYSSTIP IARFIDAILE PGKVINWNGK SVKLPPLKMC 490 500 510 520 530 540 IFAGTNPFHR HQQINRIIEG LRKLETVIAI DNQWTSTCRF ADIVLPATTQ FERNDLDQYG 550 560 570 580 590 600 NHSNRGIIAM KQVVPPQFEA RNDFDIFREL CRRFNREEAF TEGLDEMGWL KRIWQEGVQQ 610 620 630 640 650 660 GKGRGVHLPA FDDFWNNKEY VEFDHPQMFV RHQAFREDPD LEPLGTPSGL IEIYSKTIAD 670 680 690 700 710 720 MNYDDCQGHP MWFEKIERSH GGPGSQKYPL HLQSVHPDFR LHSQLCESET LRQQYTVAGK 730 740 750 760 770 780 EPVFINPQDA SARGIRNGDV VRVFNARGQV LAGAVVSDRY APGVARIHEG AWYDPDKGGE 790 800 810 820 830 840 PGALCKYGNP NVLTIDIGTS QLAQATSAHT TLVEIEKYNG TVEQVTAFNG PVEMVAQCEY VPASQVKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TMAO anaerobic respiration in Escherichia coli: involvement of the tor operon." Mejean V., Iobbi-Nivol C., Lepelletier M., Giordano G., Chippaux M., Pascal M.-C. Mol. Microbiol. 11:1169-1179(1994) [PubMed: 8022286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 40-46. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"An analogue of the DnaJ molecular chaperone in Escherichia coli." Ueguchi C., Kakeda M., Yamada H., Mizuno T. Proc. Natl. Acad. Sci. U.S.A. 91:1054-1058(1994) [PubMed: 8302830] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 767-848. Strain: K12.
[6]	"The inducible trimethylamine N-oxide reductase of Escherichia coli K12: its localization and inducers." Silvestro A., Pommier J., Pascal M.-C., Giordano G. Biochim. Biophys. Acta 999:208-216(1989) [PubMed: 2512991] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"A novel Sec-independent periplasmic protein translocation pathway in Escherichia coli." Santini C.-L., Ize B., Chanal A., Mueller M., Giordano G., Wu L.-F. EMBO J. 17:101-112(1998) [PubMed: 9427745] [Abstract] Cited for: SEC-INDEPENDENT TRANSLOCATION, PROBABLE EXPORT VIA TAT-SYSTEM.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X73888 Genomic DNA. Translation: CAA52095.1. U00096 Genomic DNA. Translation: AAC74082.1. AP009048 Genomic DNA. Translation: BAA36139.1. D16500 Genomic DNA. No translation available.
PIR	C64841.
RefSeq	AP_001628.1. NP_415517.1.
3D structure databases
HSSP	HSSP built from PDB template 1TMO based on UniProtKB O87948.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:11013N.
Protein family/group databases
TCDB	5.A.3.4.1. prokaryotic molybdopterin-containing oxidoreductase (PMO) family.
Genome annotation databases
GeneID	946267.
GenomeReviews	Gene locus JW0982 in contig AP009048_GR. Gene locus b0997 in contig U00096_GR.
KEGG	ecj:JW0982. eco:b0997.
Organism-specific databases
EchoBASE	EB1761.
EcoGene	EG11814. torA.
CMR	Search...
Phylogenomic databases
HOGENOM	P33225.
OMA	P33225. LRQQYAV.
Enzyme and pathway databases
BioCyc	EcoCyc:TORA-MON. MetaCyc:TORA-MON.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006658. BisC. IPR006656. Mopterin_OxRdtase. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR006311. Tat. IPR011887. TorA. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR00509. bisC_fam. 1 hit. TIGR01409. TAT_signal_seq. 1 hit. TIGR02164. torA. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TORA_ECOLI

Accession

Primary (citable) accession number: P33225
Secondary accession number(s): P78227

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)