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Protein

Phosphoribosylglycinamide formyltransferase 2

Gene

purT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes two reactions: the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP.1 Publication

Catalytic activityi

Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate.1 Publication

Kineticsi

Kcat is 37.6 sec(-1) for transferase activity (at pH 8).1 Publication

Manual assertion based on experiment ini

  1. KM=10.1 µM for GAR (at pH 8)1 Publication
  2. KM=319 µM for formate (at pH 8)1 Publication

    Pathwayi: IMP biosynthesis via de novo pathway

    This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route).UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. Phosphoribosylglycinamide formyltransferase 2 (purT)
    This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei825'-phosphoribosylglycinamide2 Publications1
    Binding sitei114ATP2 Publications1
    Binding sitei155ATP2 Publications1
    Binding sitei203ATP2 Publications1
    Metal bindingi267Magnesium2 Publications1
    Metal bindingi279Magnesium2 Publications1
    Binding sitei2865'-phosphoribosylglycinamide2 Publications1
    Binding sitei3555'-phosphoribosylglycinamide2 Publications1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi160 – 165ATP2 Publications6
    Nucleotide bindingi195 – 198ATP2 Publications4

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:GARTRANSFORMYL2-MONOMER.
    ECOL316407:JW1838-MONOMER.
    MetaCyc:GARTRANSFORMYL2-MONOMER.
    UniPathwayiUPA00074; UER00127.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylglycinamide formyltransferase 2UniRule annotation (EC:2.1.2.-1 Publication)
    Short name:
    GART 2UniRule annotation
    Alternative name(s):
    5'-phosphoribosylglycinamide transformylase 2UniRule annotation
    Formate-dependent GAR transformylaseUniRule annotation
    GAR transformylase 2UniRule annotation
    Gene namesi
    Name:purTUniRule annotation
    Ordered Locus Names:b1849, JW1838
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11809. purT.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00000749562 – 392Phosphoribosylglycinamide formyltransferase 2Add BLAST391

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei179N6-acetyllysineUniRule annotation1 Publication1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP33221.
    PRIDEiP33221.

    Interactioni

    Subunit structurei

    Homodimer.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ispAP229393EBI-553029,EBI-553011

    Protein-protein interaction databases

    DIPiDIP-10618N.
    IntActiP33221. 7 interactors.
    MINTiMINT-1306001.
    STRINGi511145.b1849.

    Structurei

    Secondary structure

    1392
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi14 – 19Combined sources6
    Helixi22 – 32Combined sources11
    Turni33 – 35Combined sources3
    Beta strandi37 – 44Combined sources8
    Helixi48 – 52Combined sources5
    Beta strandi53 – 58Combined sources6
    Helixi64 – 74Combined sources11
    Beta strandi77 – 81Combined sources5
    Helixi88 – 96Combined sources9
    Beta strandi100 – 103Combined sources4
    Helixi105 – 112Combined sources8
    Helixi114 – 122Combined sources9
    Beta strandi132 – 137Combined sources6
    Helixi138 – 148Combined sources11
    Beta strandi150 – 157Combined sources8
    Turni161 – 164Combined sources4
    Beta strandi166 – 168Combined sources3
    Helixi171 – 173Combined sources3
    Helixi174 – 184Combined sources11
    Helixi186 – 188Combined sources3
    Beta strandi192 – 196Combined sources5
    Beta strandi201 – 211Combined sources11
    Beta strandi214 – 217Combined sources4
    Beta strandi221 – 226Combined sources6
    Beta strandi229 – 235Combined sources7
    Helixi241 – 258Combined sources18
    Beta strandi260 – 271Combined sources12
    Beta strandi274 – 283Combined sources10
    Helixi286 – 290Combined sources5
    Helixi291 – 294Combined sources4
    Beta strandi295 – 297Combined sources3
    Helixi299 – 307Combined sources9
    Beta strandi319 – 326Combined sources8
    Beta strandi329 – 334Combined sources6
    Beta strandi336 – 338Combined sources3
    Helixi340 – 342Combined sources3
    Beta strandi348 – 352Combined sources5
    Beta strandi358 – 361Combined sources4
    Beta strandi365 – 370Combined sources6
    Helixi374 – 387Combined sources14
    Beta strandi389 – 391Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1EYZX-ray1.75A/B1-392[»]
    1EZ1X-ray1.75A/B1-392[»]
    1KJ8X-ray1.60A/B2-392[»]
    1KJ9X-ray1.60A/B2-392[»]
    1KJIX-ray1.60A/B2-392[»]
    1KJJX-ray1.75A/B2-392[»]
    1KJQX-ray1.05A/B2-392[»]
    1NFEmodel-A1-392[»]
    ProteinModelPortaliP33221.
    SMRiP33221.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP33221.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini119 – 308ATP-graspUniRule annotationAdd BLAST190

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni22 – 235'-phosphoribosylglycinamide binding2 Publications2
    Regioni362 – 3635'-phosphoribosylglycinamide binding2 Publications2

    Sequence similaritiesi

    Belongs to the PurK/PurT family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4108HH9. Bacteria.
    COG0027. LUCA.
    HOGENOMiHOG000072820.
    InParanoidiP33221.
    KOiK08289.
    OMAiIFGVEFF.
    PhylomeDBiP33221.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPiMF_01643. PurT. 1 hit.
    InterProiIPR011761. ATP-grasp.
    IPR003135. ATP-grasp_carboxylate-amine.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR005862. PurT.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF02222. ATP-grasp. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01142. purT. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33221-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTLLGTALRP AATRVMLLGS GELGKEVAIE CQRLGVEVIA VDRYADAPAM
    60 70 80 90 100
    HVAHRSHVIN MLDGDALRRV VELEKPHYIV PEIEAIATDM LIQLEEEGLN
    110 120 130 140 150
    VVPCARATKL TMNREGIRRL AAEELQLPTS TYRFADSESL FREAVADIGY
    160 170 180 190 200
    PCIVKPVMSS SGKGQTFIRS AEQLAQAWKY AQQGGRAGAG RVIVEGVVKF
    210 220 230 240 250
    DFEITLLTVS AVDGVHFCAP VGHRQEDGDY RESWQPQQMS PLALERAQEI
    260 270 280 290 300
    ARKVVLALGG YGLFGVELFV CGDEVIFSEV SPRPHDTGMV TLISQDLSEF
    310 320 330 340 350
    ALHVRAFLGL PVGGIRQYGP AASAVILPQL TSQNVTFDNV QNAVGADLQI
    360 370 380 390
    RLFGKPEIDG SRRLGVALAT AESVVDAIER AKHAAGQVKV QG
    Length:392
    Mass (Da):42,434
    Last modified:January 23, 2007 - v3
    Checksum:i276B3883E7403EA9
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L20897 Genomic DNA. Translation: AAA23861.1.
    U00096 Genomic DNA. Translation: AAC74919.1.
    AP009048 Genomic DNA. Translation: BAA15657.1.
    PIRiA54227.
    RefSeqiNP_416363.1. NC_000913.3.
    WP_000173484.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74919; AAC74919; b1849.
    BAA15657; BAA15657; BAA15657.
    GeneIDi946368.
    KEGGiecj:JW1838.
    eco:b1849.
    PATRICi32119019. VBIEscCol129921_1927.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L20897 Genomic DNA. Translation: AAA23861.1.
    U00096 Genomic DNA. Translation: AAC74919.1.
    AP009048 Genomic DNA. Translation: BAA15657.1.
    PIRiA54227.
    RefSeqiNP_416363.1. NC_000913.3.
    WP_000173484.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1EYZX-ray1.75A/B1-392[»]
    1EZ1X-ray1.75A/B1-392[»]
    1KJ8X-ray1.60A/B2-392[»]
    1KJ9X-ray1.60A/B2-392[»]
    1KJIX-ray1.60A/B2-392[»]
    1KJJX-ray1.75A/B2-392[»]
    1KJQX-ray1.05A/B2-392[»]
    1NFEmodel-A1-392[»]
    ProteinModelPortaliP33221.
    SMRiP33221.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-10618N.
    IntActiP33221. 7 interactors.
    MINTiMINT-1306001.
    STRINGi511145.b1849.

    Proteomic databases

    PaxDbiP33221.
    PRIDEiP33221.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC74919; AAC74919; b1849.
    BAA15657; BAA15657; BAA15657.
    GeneIDi946368.
    KEGGiecj:JW1838.
    eco:b1849.
    PATRICi32119019. VBIEscCol129921_1927.

    Organism-specific databases

    EchoBASEiEB1757.
    EcoGeneiEG11809. purT.

    Phylogenomic databases

    eggNOGiENOG4108HH9. Bacteria.
    COG0027. LUCA.
    HOGENOMiHOG000072820.
    InParanoidiP33221.
    KOiK08289.
    OMAiIFGVEFF.
    PhylomeDBiP33221.

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00127.
    BioCyciEcoCyc:GARTRANSFORMYL2-MONOMER.
    ECOL316407:JW1838-MONOMER.
    MetaCyc:GARTRANSFORMYL2-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiP33221.
    PROiP33221.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPiMF_01643. PurT. 1 hit.
    InterProiIPR011761. ATP-grasp.
    IPR003135. ATP-grasp_carboxylate-amine.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR005862. PurT.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF02222. ATP-grasp. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01142. purT. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPURT_ECOLI
    AccessioniPrimary (citable) accession number: P33221
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: January 23, 2007
    Last modified: November 2, 2016
    This is version 160 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.