ID   MTLK_RHOSH              Reviewed;         477 AA.
AC   P33216;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 46.
DE   RecName: Full=Mannitol 2-dehydrogenase;
DE            Short=M2DH;
DE            Short=MDH;
DE            EC=1.1.1.67;
GN   Name=mtlK;
OS   Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=1063;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-44.
RC   STRAIN=SI-4;
RX   MEDLINE=94075966; PubMed=8254318;
RA   Schneider K.-H., Giffhorn F., Kaplan S.;
RT   "Cloning, nucleotide sequence and characterization of the mannitol
RT   dehydrogenase gene from Rhodobacter sphaeroides.";
RL   J. Gen. Microbiol. 139:2475-2484(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SI-4;
RA   Schneider K.-H., Giffhorn F.;
RL   Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: D-mannitol + NAD(+) = D-fructose + NADH.
CC   -!- SUBUNIT: Monomer.
CC   -!- SIMILARITY: Belongs to the mannitol dehydrogenase family.
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DR   EMBL; AF018073; AAC45771.1; -; Genomic_DNA.
DR   HSSP; O08355; 1LJ8.
DR   BRENDA; 1.1.1.67; 2796.
DR   GO; GO:0050662; F:coenzyme binding; IEA:InterPro.
DR   GO; GO:0050086; F:mannitol 2-dehydrogenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR013118; Mannitol_DH_C.
DR   InterPro; IPR000669; Mannitol_DH_core.
DR   InterPro; IPR013131; Mannitol_DH_N.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   Pfam; PF01232; Mannitol_dh; 1.
DR   Pfam; PF08125; Mannitol_dh_C; 1.
DR   PRINTS; PR00084; MTLDHDRGNASE.
DR   PROSITE; PS00974; MANNITOL_DHGENASE; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; NAD; Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    477       Mannitol 2-dehydrogenase.
FT                                /FTId=PRO_0000170734.
FT   NP_BIND      19     30       NAD (By similarity).
SQ   SEQUENCE   477 AA;  51538 MW;  F823623B7BA920E8 CRC64;
     MTRSVTRPSY DRKALTPGIV HIGVGNFHRA HQAVYLDDLF ALGEGHDWAI LGAGVRPTDA
     RMREALAAQD NLSTVIELDP AGHRARQVGA MVGFLPVEAD NAALIEAMSD PRIRIVSLTV
     TEGGYYVDAS GAFDPTHPDI VADAAHPARP ATAFGAILAA LRARRDAGVT PFTVMSCDNL
     PGNGHVTRNA VVGLAELYDA ELAGWVKAQV AFPNGMVDRI TPATGPHERE LAQGFGLADP
     VPVTCEPFRQ WVIEDHFPAG RPALEKVGVT FTPHVHAYEA MKIRILNGGH AVIAYPSALM
     DIQLVHAAMA HPLIAAFLHK VEVEEILPHV PPVPDTSIPD YLTLIESRFS NPEIADTTRR
     LCLDGSNRQP KFIVPSLRDN LAAGTVPKGL VLLSALWCRY CFGTTDSGVV VEPNDPNWTA
     LQDRARRAKE TPAEWLAMTE VYGDLAQNDL LAAEFAAALE AVWRDGAEAV LRRFLAA
//