Reviewed, UniProtKB/Swiss-Prot P33216 (MTLK_RHOSH)

Names and origin

Protein names

Recommended name:
    Mannitol 2-dehydrogenase
      Short name=M2DH
      Short name=MDH
    EC=1.1.1.67

Gene names

Name:

mtlK

Organism

Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)

Taxonomic identifier

1063 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-mannitol + NAD+ = D-fructose + NADH.
Subunit structure	Monomer.
Sequence similarities	Belongs to the mannitol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro mannitol 2-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 477	476	Mannitol 2-dehydrogenase		PRO_0000170734
Regions
	Nucleotide binding	19 – 30	12	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33216-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F823623B7BA920E8	FASTA	477	51,538
	10 20 30 40 50 60 MTRSVTRPSY DRKALTPGIV HIGVGNFHRA HQAVYLDDLF ALGEGHDWAI LGAGVRPTDA 70 80 90 100 110 120 RMREALAAQD NLSTVIELDP AGHRARQVGA MVGFLPVEAD NAALIEAMSD PRIRIVSLTV 130 140 150 160 170 180 TEGGYYVDAS GAFDPTHPDI VADAAHPARP ATAFGAILAA LRARRDAGVT PFTVMSCDNL 190 200 210 220 230 240 PGNGHVTRNA VVGLAELYDA ELAGWVKAQV AFPNGMVDRI TPATGPHERE LAQGFGLADP 250 260 270 280 290 300 VPVTCEPFRQ WVIEDHFPAG RPALEKVGVT FTPHVHAYEA MKIRILNGGH AVIAYPSALM 310 320 330 340 350 360 DIQLVHAAMA HPLIAAFLHK VEVEEILPHV PPVPDTSIPD YLTLIESRFS NPEIADTTRR 370 380 390 400 410 420 LCLDGSNRQP KFIVPSLRDN LAAGTVPKGL VLLSALWCRY CFGTTDSGVV VEPNDPNWTA 430 440 450 460 470 LQDRARRAKE TPAEWLAMTE VYGDLAQNDL LAAEFAAALE AVWRDGAEAV LRRFLAA

References

[1]	"Cloning, nucleotide sequence and characterization of the mannitol dehydrogenase gene from Rhodobacter sphaeroides." Schneider K.-H., Giffhorn F., Kaplan S. J. Gen. Microbiol. 139:2475-2484(1993) [PubMed: 8254318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-44. Strain: SI-4.
[2]	Schneider K.-H., Giffhorn F. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SI-4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF018073 Genomic DNA. Translation: AAC45771.1.
3D structure databases
HSSP	HSSP built from PDB template 1LJ8 based on UniProtKB O08355.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.67. 2796.
Family and domain databases
InterPro	IPR013328. DH_multihelical. IPR013118. Mannitol_DH_C. IPR000669. Mannitol_DH_core. IPR013131. Mannitol_DH_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF01232. Mannitol_dh. 1 hit. PF08125. Mannitol_dh_C. 1 hit. [Graphical view]
PRINTS	PR00084. MTLDHDRGNASE.
PROSITE	PS00974. MANNITOL_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTLK_RHOSH

Accession

Primary (citable) accession number: P33216

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 46 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)