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Protein

Mannitol 2-dehydrogenase

Gene

mtlK

Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-mannitol + NAD+ = D-fructose + NADH.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 3012NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. coenzyme binding Source: InterPro
  2. mannitol 2-dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. mannitol metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Mannitol 2-dehydrogenase (EC:1.1.1.67)
Short name:
M2DH
Short name:
MDH
Gene namesi
Name:mtlK
OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifieri1063 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 477476Mannitol 2-dehydrogenasePRO_0000170734Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP33216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mannitol dehydrogenase family.Curated

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR000669. Mannitol_DH.
IPR013118. Mannitol_DH_C.
IPR023027. Mannitol_DH_CS.
IPR013131. Mannitol_DH_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01232. Mannitol_dh. 1 hit.
PF08125. Mannitol_dh_C. 1 hit.
[Graphical view]
PRINTSiPR00084. MTLDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
PROSITEiPS00974. MANNITOL_DHGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33216-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTRSVTRPSY DRKALTPGIV HIGVGNFHRA HQAVYLDDLF ALGEGHDWAI
60 70 80 90 100
LGAGVRPTDA RMREALAAQD NLSTVIELDP AGHRARQVGA MVGFLPVEAD
110 120 130 140 150
NAALIEAMSD PRIRIVSLTV TEGGYYVDAS GAFDPTHPDI VADAAHPARP
160 170 180 190 200
ATAFGAILAA LRARRDAGVT PFTVMSCDNL PGNGHVTRNA VVGLAELYDA
210 220 230 240 250
ELAGWVKAQV AFPNGMVDRI TPATGPHERE LAQGFGLADP VPVTCEPFRQ
260 270 280 290 300
WVIEDHFPAG RPALEKVGVT FTPHVHAYEA MKIRILNGGH AVIAYPSALM
310 320 330 340 350
DIQLVHAAMA HPLIAAFLHK VEVEEILPHV PPVPDTSIPD YLTLIESRFS
360 370 380 390 400
NPEIADTTRR LCLDGSNRQP KFIVPSLRDN LAAGTVPKGL VLLSALWCRY
410 420 430 440 450
CFGTTDSGVV VEPNDPNWTA LQDRARRAKE TPAEWLAMTE VYGDLAQNDL
460 470
LAAEFAAALE AVWRDGAEAV LRRFLAA
Length:477
Mass (Da):51,538
Last modified:January 23, 2007 - v3
Checksum:iF823623B7BA920E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF018073 Genomic DNA. Translation: AAC45771.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF018073 Genomic DNA. Translation: AAC45771.1.

3D structure databases

ProteinModelPortaliP33216.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR000669. Mannitol_DH.
IPR013118. Mannitol_DH_C.
IPR023027. Mannitol_DH_CS.
IPR013131. Mannitol_DH_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01232. Mannitol_dh. 1 hit.
PF08125. Mannitol_dh_C. 1 hit.
[Graphical view]
PRINTSiPR00084. MTLDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
PROSITEiPS00974. MANNITOL_DHGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, nucleotide sequence and characterization of the mannitol dehydrogenase gene from Rhodobacter sphaeroides."
    Schneider K.-H., Giffhorn F., Kaplan S.
    J. Gen. Microbiol. 139:2475-2484(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-44.
    Strain: SI-4.
  2. Schneider K.-H., Giffhorn F.
    Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SI-4.

Entry informationi

Entry nameiMTLK_RHOSH
AccessioniPrimary (citable) accession number: P33216
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 66 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.