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Protein

Protein NEDD1

Gene

Nedd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle (By similarity). May play an important role during the embryonic development and differentiation of the central nervous system.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-380320. Recruitment of NuMA to mitotic centrosomes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NEDD1
Alternative name(s):
Neural precursor cell expressed developmentally down-regulated protein 1
Short name:
NEDD-1
Gene namesi
Name:Nedd1
Synonyms:Nedd-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97293. Nedd1.

Subcellular locationi

GO - Cellular componenti

  • apical part of cell Source: MGI
  • centriole Source: MGI
  • centrosome Source: MGI
  • ciliary basal body Source: MGI
  • cytoplasm Source: MGI
  • gamma-tubulin ring complex, centrosomal Source: MGI
  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • pericentriolar material Source: MGI
  • plasma membrane Source: MGI
  • spindle pole Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 660660Protein NEDD1PRO_0000051096Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei379 – 3791PhosphoserineCombined sources
Modified residuei397 – 3971Phosphoserine; by PLK1By similarity
Modified residuei411 – 4111PhosphoserineCombined sources
Modified residuei426 – 4261Phosphoserine; by PLK1By similarity
Modified residuei550 – 5501Phosphothreonine; by CDK1By similarity
Modified residuei637 – 6371Phosphoserine; by PLK1By similarity

Post-translational modificationi

During mitosis, prior phosphorylation on Thr-550 by CDK1 promotes subsequent phosphorylation by PLK1 on Ser-397, Ser-426 and Ser-637. Phosphorylated NEDD1 can interact with gamma-tubulin for targeting the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP33215.
MaxQBiP33215.
PaxDbiP33215.
PRIDEiP33215.

PTM databases

PhosphoSiteiP33215.

Expressioni

Developmental stagei

Down-regulated during the development of brain.

Gene expression databases

BgeeiP33215.
GenevisibleiP33215. MM.

Interactioni

Subunit structurei

Interacts with FAM29A and gamma-tubulin.By similarity

Protein-protein interaction databases

BioGridi201721. 106 interactions.
IntActiP33215. 106 interactions.
STRINGi10090.ENSMUSP00000020163.

Structurei

3D structure databases

ProteinModelPortaliP33215.
SMRiP33215. Positions 7-317.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 3131WD 1Add
BLAST
Repeati32 – 7140WD 2Add
BLAST
Repeati75 – 11440WD 3Add
BLAST
Repeati117 – 15640WD 4Add
BLAST
Repeati160 – 20041WD 5Add
BLAST
Repeati204 – 24441WD 6Add
BLAST
Repeati246 – 28540WD 7Add
BLAST
Repeati289 – 33244WD 8Add
BLAST

Sequence similaritiesi

Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4378. Eukaryota.
ENOG410ZQNY. LUCA.
GeneTreeiENSGT00390000001561.
HOGENOMiHOG000034642.
HOVERGENiHBG055623.
InParanoidiP33215.
KOiK16547.
OMAiRYSVNES.
OrthoDBiEOG79KPDX.
PhylomeDBiP33215.
TreeFamiTF329816.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P33215-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQENLRFASS GDDVKIWDAS FLTLVDKFNP HTSPHGISSI CWSSNNNFLV
60 70 80 90 100
TASSSGDKIV VSSCKCKPVP LLELAEGQKQ TCVDLNSTSM YLASGGLNNT
110 120 130 140 150
VNIWDLKSKR LHRSLKDHKC EVTCVAYNWN DCYIASGSLS GEIILHSVTT
160 170 180 190 200
NTSSTPFGHG SKQPIRHIKY SLFRKSLLGS VSDNGVVTLW DVNSQSSYHT
210 220 230 240 250
FDSTHKAPAS GICFSPVNEL LFVTIGLDKR IILYDTSSKK LVKTLVADTP
260 270 280 290 300
LTAVDFMPDG ATLAIGSSRG KIYQYDLRML KSPVKTISAH KTSVQCIAFQ
310 320 330 340 350
YSTSLTKASL SKGSSNKATA VNKRSVPVSS SSGAAQNSGI VREAPSPSIA
360 370 380 390 400
TVLPQPVTTA LGKGSGAAQD EAGLARSKST DIFSKETDAG KSQDFSSFDD
410 420 430 440 450
TGKNSLGDMF SPIRDDAVVS KGGDESIGKG DGLDFLPQLN SVFPLRKNAG
460 470 480 490 500
ASSSLVLHSS PLNVLMGSSG KEENESHEPS AESKRAYLGK QEPKDAMKQF
510 520 530 540 550
TKLISSGAEP GILNTCPSSN QARNLEKFEK PEKDIEAQLI HEPSVNGSST
560 570 580 590 600
TVPKAASSVT AGVASSLSEK IVDTLGNSRP GAPLTSVQIR FIQNMIQETL
610 620 630 640 650
DDFREACHRD IVNLQVEMIK QFHIQLNEMH SLLERYSVNE GLVAEIERLR
660
EENKRLRAHF
Length:660
Mass (Da):71,292
Last modified:November 22, 2005 - v2
Checksum:iE26CACECE07344BF
GO
Isoform 2 (identifier: P33215-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     500-508: FTKLISSGA → VSPRKEYPV
     509-660: Missing.

Note: No experimental confirmation available.
Show »
Length:508
Mass (Da):54,383
Checksum:iCB722722130E74D7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131D → G in AAH66870 (PubMed:15489334).Curated
Sequence conflicti187 – 1871V → E in BAC41060 (PubMed:16141072).Curated
Sequence conflicti370 – 3701D → G in BAC33321 (PubMed:16141072).Curated
Sequence conflicti479 – 4791P → L in BAA01554 (PubMed:1378265).Curated
Sequence conflicti479 – 4791P → L in BAC41060 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei500 – 5089FTKLISSGA → VSPRKEYPV in isoform 2. 1 PublicationVSP_016263
Alternative sequencei509 – 660152Missing in isoform 2. 1 PublicationVSP_016264Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10712 mRNA. Translation: BAA01554.1.
AK048393 mRNA. Translation: BAC33321.1.
AK085355 mRNA. Translation: BAC39429.2.
AK090040 mRNA. Translation: BAC41060.1.
AK010591 mRNA. Translation: BAB27048.1.
AK153746 mRNA. Translation: BAE32166.1.
BC052430 mRNA. Translation: AAH52430.1.
BC066870 mRNA. Translation: AAH66870.1.
CCDSiCCDS24123.1. [P33215-1]
PIRiI60167.
RefSeqiNP_032708.2. NM_008682.2. [P33215-1]
UniGeneiMm.2998.

Genome annotation databases

EnsembliENSMUST00000020163; ENSMUSP00000020163; ENSMUSG00000019988. [P33215-1]
GeneIDi17997.
KEGGimmu:17997.
UCSCiuc007gud.2. mouse. [P33215-1]
uc007gue.2. mouse. [P33215-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10712 mRNA. Translation: BAA01554.1.
AK048393 mRNA. Translation: BAC33321.1.
AK085355 mRNA. Translation: BAC39429.2.
AK090040 mRNA. Translation: BAC41060.1.
AK010591 mRNA. Translation: BAB27048.1.
AK153746 mRNA. Translation: BAE32166.1.
BC052430 mRNA. Translation: AAH52430.1.
BC066870 mRNA. Translation: AAH66870.1.
CCDSiCCDS24123.1. [P33215-1]
PIRiI60167.
RefSeqiNP_032708.2. NM_008682.2. [P33215-1]
UniGeneiMm.2998.

3D structure databases

ProteinModelPortaliP33215.
SMRiP33215. Positions 7-317.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201721. 106 interactions.
IntActiP33215. 106 interactions.
STRINGi10090.ENSMUSP00000020163.

PTM databases

PhosphoSiteiP33215.

Proteomic databases

EPDiP33215.
MaxQBiP33215.
PaxDbiP33215.
PRIDEiP33215.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020163; ENSMUSP00000020163; ENSMUSG00000019988. [P33215-1]
GeneIDi17997.
KEGGimmu:17997.
UCSCiuc007gud.2. mouse. [P33215-1]
uc007gue.2. mouse. [P33215-2]

Organism-specific databases

CTDi121441.
MGIiMGI:97293. Nedd1.

Phylogenomic databases

eggNOGiKOG4378. Eukaryota.
ENOG410ZQNY. LUCA.
GeneTreeiENSGT00390000001561.
HOGENOMiHOG000034642.
HOVERGENiHBG055623.
InParanoidiP33215.
KOiK16547.
OMAiRYSVNES.
OrthoDBiEOG79KPDX.
PhylomeDBiP33215.
TreeFamiTF329816.

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-380320. Recruitment of NuMA to mitotic centrosomes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.

Miscellaneous databases

NextBioi292991.
PROiP33215.
SOURCEiSearch...

Gene expression databases

BgeeiP33215.
GenevisibleiP33215. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a set of genes with developmentally down-regulated expression in the mouse brain."
    Kumar S., Tomooka Y., Noda M.
    Biochem. Biophys. Res. Commun. 185:1155-1161(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Head and Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain and Egg.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-379 AND SER-411, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung and Spleen.

Entry informationi

Entry nameiNEDD1_MOUSE
AccessioniPrimary (citable) accession number: P33215
Secondary accession number(s): Q6NXV3
, Q8BN12, Q8BN86, Q8BQL9, Q9CWK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 22, 2005
Last modified: May 11, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.