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Protein

Protein NEDD1

Gene

Nedd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle (By similarity). May play an important role during the embryonic development and differentiation of the central nervous system.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-380320. Recruitment of NuMA to mitotic centrosomes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NEDD1
Alternative name(s):
Neural precursor cell expressed developmentally down-regulated protein 1
Short name:
NEDD-1
Gene namesi
Name:Nedd1
Synonyms:Nedd-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97293. Nedd1.

Subcellular locationi

GO - Cellular componenti

  • apical part of cell Source: MGI
  • centriole Source: MGI
  • centrosome Source: MGI
  • ciliary basal body Source: MGI
  • cytoplasm Source: MGI
  • gamma-tubulin ring complex, centrosomal Source: MGI
  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • pericentriolar material Source: MGI
  • plasma membrane Source: MGI
  • spindle pole Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510961 – 660Protein NEDD1Add BLAST660

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei325PhosphoserineBy similarity1
Modified residuei379PhosphoserineCombined sources1
Modified residuei397Phosphoserine; by PLK1By similarity1
Modified residuei411PhosphoserineCombined sources1
Modified residuei426Phosphoserine; by PLK1By similarity1
Modified residuei468PhosphoserineBy similarity1
Modified residuei550Phosphothreonine; by CDK1By similarity1
Modified residuei637Phosphoserine; by PLK1By similarity1

Post-translational modificationi

During mitosis, prior phosphorylation on Thr-550 by CDK1 promotes subsequent phosphorylation by PLK1 on Ser-397, Ser-426 and Ser-637. Phosphorylated NEDD1 can interact with gamma-tubulin for targeting the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP33215.
MaxQBiP33215.
PaxDbiP33215.
PeptideAtlasiP33215.
PRIDEiP33215.

PTM databases

iPTMnetiP33215.
PhosphoSitePlusiP33215.

Expressioni

Developmental stagei

Down-regulated during the development of brain.

Gene expression databases

BgeeiENSMUSG00000019988.
GenevisibleiP33215. MM.

Interactioni

Subunit structurei

Interacts with FAM29A and gamma-tubulin.By similarity

Protein-protein interaction databases

BioGridi201721. 106 interactors.
IntActiP33215. 106 interactors.
STRINGi10090.ENSMUSP00000020163.

Structurei

3D structure databases

ProteinModelPortaliP33215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 31WD 1Add BLAST31
Repeati32 – 71WD 2Add BLAST40
Repeati75 – 114WD 3Add BLAST40
Repeati117 – 156WD 4Add BLAST40
Repeati160 – 200WD 5Add BLAST41
Repeati204 – 244WD 6Add BLAST41
Repeati246 – 285WD 7Add BLAST40
Repeati289 – 332WD 8Add BLAST44

Sequence similaritiesi

Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4378. Eukaryota.
ENOG410ZQNY. LUCA.
GeneTreeiENSGT00390000001561.
HOGENOMiHOG000034642.
HOVERGENiHBG055623.
InParanoidiP33215.
KOiK16547.
OMAiRYSVNES.
OrthoDBiEOG091G07XS.
PhylomeDBiP33215.
TreeFamiTF329816.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P33215-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQENLRFASS GDDVKIWDAS FLTLVDKFNP HTSPHGISSI CWSSNNNFLV
60 70 80 90 100
TASSSGDKIV VSSCKCKPVP LLELAEGQKQ TCVDLNSTSM YLASGGLNNT
110 120 130 140 150
VNIWDLKSKR LHRSLKDHKC EVTCVAYNWN DCYIASGSLS GEIILHSVTT
160 170 180 190 200
NTSSTPFGHG SKQPIRHIKY SLFRKSLLGS VSDNGVVTLW DVNSQSSYHT
210 220 230 240 250
FDSTHKAPAS GICFSPVNEL LFVTIGLDKR IILYDTSSKK LVKTLVADTP
260 270 280 290 300
LTAVDFMPDG ATLAIGSSRG KIYQYDLRML KSPVKTISAH KTSVQCIAFQ
310 320 330 340 350
YSTSLTKASL SKGSSNKATA VNKRSVPVSS SSGAAQNSGI VREAPSPSIA
360 370 380 390 400
TVLPQPVTTA LGKGSGAAQD EAGLARSKST DIFSKETDAG KSQDFSSFDD
410 420 430 440 450
TGKNSLGDMF SPIRDDAVVS KGGDESIGKG DGLDFLPQLN SVFPLRKNAG
460 470 480 490 500
ASSSLVLHSS PLNVLMGSSG KEENESHEPS AESKRAYLGK QEPKDAMKQF
510 520 530 540 550
TKLISSGAEP GILNTCPSSN QARNLEKFEK PEKDIEAQLI HEPSVNGSST
560 570 580 590 600
TVPKAASSVT AGVASSLSEK IVDTLGNSRP GAPLTSVQIR FIQNMIQETL
610 620 630 640 650
DDFREACHRD IVNLQVEMIK QFHIQLNEMH SLLERYSVNE GLVAEIERLR
660
EENKRLRAHF
Length:660
Mass (Da):71,292
Last modified:November 22, 2005 - v2
Checksum:iE26CACECE07344BF
GO
Isoform 2 (identifier: P33215-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     500-508: FTKLISSGA → VSPRKEYPV
     509-660: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:508
Mass (Da):54,383
Checksum:iCB722722130E74D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13D → G in AAH66870 (PubMed:15489334).Curated1
Sequence conflicti187V → E in BAC41060 (PubMed:16141072).Curated1
Sequence conflicti370D → G in BAC33321 (PubMed:16141072).Curated1
Sequence conflicti479P → L in BAA01554 (PubMed:1378265).Curated1
Sequence conflicti479P → L in BAC41060 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016263500 – 508FTKLISSGA → VSPRKEYPV in isoform 2. 1 Publication9
Alternative sequenceiVSP_016264509 – 660Missing in isoform 2. 1 PublicationAdd BLAST152

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10712 mRNA. Translation: BAA01554.1.
AK048393 mRNA. Translation: BAC33321.1.
AK085355 mRNA. Translation: BAC39429.2.
AK090040 mRNA. Translation: BAC41060.1.
AK010591 mRNA. Translation: BAB27048.1.
AK153746 mRNA. Translation: BAE32166.1.
BC052430 mRNA. Translation: AAH52430.1.
BC066870 mRNA. Translation: AAH66870.1.
CCDSiCCDS24123.1. [P33215-1]
PIRiI60167.
RefSeqiNP_032708.2. NM_008682.2. [P33215-1]
UniGeneiMm.2998.

Genome annotation databases

EnsembliENSMUST00000020163; ENSMUSP00000020163; ENSMUSG00000019988. [P33215-1]
GeneIDi17997.
KEGGimmu:17997.
UCSCiuc007gud.2. mouse. [P33215-1]
uc007gue.2. mouse. [P33215-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10712 mRNA. Translation: BAA01554.1.
AK048393 mRNA. Translation: BAC33321.1.
AK085355 mRNA. Translation: BAC39429.2.
AK090040 mRNA. Translation: BAC41060.1.
AK010591 mRNA. Translation: BAB27048.1.
AK153746 mRNA. Translation: BAE32166.1.
BC052430 mRNA. Translation: AAH52430.1.
BC066870 mRNA. Translation: AAH66870.1.
CCDSiCCDS24123.1. [P33215-1]
PIRiI60167.
RefSeqiNP_032708.2. NM_008682.2. [P33215-1]
UniGeneiMm.2998.

3D structure databases

ProteinModelPortaliP33215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201721. 106 interactors.
IntActiP33215. 106 interactors.
STRINGi10090.ENSMUSP00000020163.

PTM databases

iPTMnetiP33215.
PhosphoSitePlusiP33215.

Proteomic databases

EPDiP33215.
MaxQBiP33215.
PaxDbiP33215.
PeptideAtlasiP33215.
PRIDEiP33215.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020163; ENSMUSP00000020163; ENSMUSG00000019988. [P33215-1]
GeneIDi17997.
KEGGimmu:17997.
UCSCiuc007gud.2. mouse. [P33215-1]
uc007gue.2. mouse. [P33215-2]

Organism-specific databases

CTDi121441.
MGIiMGI:97293. Nedd1.

Phylogenomic databases

eggNOGiKOG4378. Eukaryota.
ENOG410ZQNY. LUCA.
GeneTreeiENSGT00390000001561.
HOGENOMiHOG000034642.
HOVERGENiHBG055623.
InParanoidiP33215.
KOiK16547.
OMAiRYSVNES.
OrthoDBiEOG091G07XS.
PhylomeDBiP33215.
TreeFamiTF329816.

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-380320. Recruitment of NuMA to mitotic centrosomes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Miscellaneous databases

PROiP33215.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019988.
GenevisibleiP33215. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEDD1_MOUSE
AccessioniPrimary (citable) accession number: P33215
Secondary accession number(s): Q6NXV3
, Q8BN12, Q8BN86, Q8BQL9, Q9CWK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 22, 2005
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.