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Protein

3-oxoacyl-[acyl-carrier-protein] reductase, chloroplastic

Gene

At1g24360

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei213 – 2131SubstrateBy similarity
Active sitei226 – 2261Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi81 – 10525NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: TAIR
  2. copper ion binding Source: TAIR
  3. NAD binding Source: InterPro

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT1G24360-MONOMER.
MetaCyc:AT1G24360-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase, chloroplastic (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Gene namesi
Ordered Locus Names:At1g24360
ORF Names:F21J9.2, F3I6.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G24360.

Subcellular locationi

Plastidchloroplast. Plastid
Note: And non-photosynthetic plastids.

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. chloroplast stroma Source: TAIR
  4. plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5959ChloroplastBy similarityAdd
BLAST
Chaini60 – 3192603-oxoacyl-[acyl-carrier-protein] reductase, chloroplasticPRO_0000031976Add
BLAST

Proteomic databases

PaxDbiP33207.
PRIDEiP33207.

Expressioni

Gene expression databases

GenevestigatoriP33207.

Interactioni

Subunit structurei

Homotetramer.Curated

Protein-protein interaction databases

STRINGi3702.AT1G24360.1-P.

Structurei

3D structure databases

ProteinModelPortaliP33207.
SMRiP33207. Positions 23-319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiP33207.
KOiK00059.
OMAiANACLFF.
PhylomeDBiP33207.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33207-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAAVAAPRL ISLKAVAKLG FREISQIRQL APLHSAIPHF GMLRCRSRQP
60 70 80 90 100
FSTSVVKAQA TATEQSPGEV VQKVESPVVV ITGASRGIGK AIALALGKAG
110 120 130 140 150
CKVLVNYARS AKEAEEVAKQ IEEYGGQAIT FGGDVSKATD VDAMMKTALD
160 170 180 190 200
KWGTIDVVVN NAGITRDTLL IRMKQSQWDE VIALNLTGVF LCTQAAVKIM
210 220 230 240 250
MKKKRGRIIN ISSVVGLIGN IGQANYAAAK GGVISFSKTA AREGASRNIN
260 270 280 290 300
VNVVCPGFIA SDMTAELGED MEKKILGTIP LGRYGKAEEV AGLVEFLALS
310
PAASYITGQA FTIDGGIAI
Length:319
Mass (Da):33,548
Last modified:January 10, 2003 - v2
Checksum:i755ADDD16F3F69DE
GO

Sequence cautioni

The sequence AAC00590.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAF97951.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171A → G in CAA45794. (PubMed:1575676)Curated
Sequence conflicti227 – 2293AAA → TAT in CAA45794. (PubMed:1575676)Curated
Sequence conflicti238 – 2403KTA → ETP in CAA45794. (PubMed:1575676)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64464 mRNA. Translation: CAA45794.1.
AC000103 Genomic DNA. Translation: AAF97951.1. Sequence problems.
AC002396 Genomic DNA. Translation: AAC00590.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30522.1.
AF324985 mRNA. Translation: AAG40337.1.
AY059816 mRNA. Translation: AAL24298.1.
AY081491 mRNA. Translation: AAM10053.1.
PIRiA86378.
S22416.
T00667.
RefSeqiNP_564216.1. NM_102282.3.
UniGeneiAt.4.
At.67665.

Genome annotation databases

EnsemblPlantsiAT1G24360.1; AT1G24360.1; AT1G24360.
GeneIDi839053.
KEGGiath:AT1G24360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64464 mRNA. Translation: CAA45794.1.
AC000103 Genomic DNA. Translation: AAF97951.1. Sequence problems.
AC002396 Genomic DNA. Translation: AAC00590.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30522.1.
AF324985 mRNA. Translation: AAG40337.1.
AY059816 mRNA. Translation: AAL24298.1.
AY081491 mRNA. Translation: AAM10053.1.
PIRiA86378.
S22416.
T00667.
RefSeqiNP_564216.1. NM_102282.3.
UniGeneiAt.4.
At.67665.

3D structure databases

ProteinModelPortaliP33207.
SMRiP33207. Positions 23-319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G24360.1-P.

Proteomic databases

PaxDbiP33207.
PRIDEiP33207.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G24360.1; AT1G24360.1; AT1G24360.
GeneIDi839053.
KEGGiath:AT1G24360.

Organism-specific databases

TAIRiAT1G24360.

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiP33207.
KOiK00059.
OMAiANACLFF.
PhylomeDBiP33207.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciARA:AT1G24360-MONOMER.
MetaCyc:AT1G24360-MONOMER.

Miscellaneous databases

PROiP33207.

Gene expression databases

GenevestigatoriP33207.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase. Sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti."
    Slabas A.R., Chase D., Nishida I., Murata N., Sidebottom C.M., Safford R., Sheldon P.S., Kekwick R.G.O., Hardie D.G., Mackintosh R.W.
    Biochem. J. 283:321-326(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiFABG_ARATH
AccessioniPrimary (citable) accession number: P33207
Secondary accession number(s): O04463, Q9FPJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 10, 2003
Last modified: January 7, 2015
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.