Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin fusion degradation protein 4

Gene

UFD4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.By similarity

Miscellaneous

A cysteine residue is required for ubiquitin-thioester formation.
Present with 7380 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1450Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • free ubiquitin chain polymerization Source: SGD
  • negative regulation of telomerase activity Source: SGD
  • protein monoubiquitination Source: SGD
  • protein polyubiquitination Source: SGD

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31819-MONOMER
ReactomeiR-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin fusion degradation protein 4 (EC:2.3.2.-)
Short name:
UB fusion protein 4
Alternative name(s):
HECT-type E3 ubiquitin transferase UFD4 (EC:2.3.2.26)
Gene namesi
Name:UFD4
Ordered Locus Names:YKL010C
ORF Names:YKL162
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL010C
SGDiS000001493 UFD4

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949981 – 1483Ubiquitin fusion degradation protein 4Add BLAST1483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei87PhosphothreonineCombined sources1
Cross-linki349Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP33202
PaxDbiP33202
PRIDEiP33202

PTM databases

iPTMnetiP33202

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34122, 131 interactors
DIPiDIP-4873N
IntActiP33202, 30 interactors
MINTiP33202
STRINGi4932.YKL010C

Structurei

3D structure databases

ProteinModelPortaliP33202
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1376 – 1483HECTPROSITE-ProRule annotationAdd BLAST108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1007 – 1081K-boxAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi898 – 901Poly-Glu4

Sequence similaritiesi

Belongs to the UPL family. K-HECT subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063470
HOGENOMiHOG000142004
InParanoidiP33202
KOiK10590
OMAiFTFARHV
OrthoDBiEOG092C09RX

Family and domain databases

CDDicd00078 HECTc, 1 hit
Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
PfamiView protein in Pfam
PF00632 HECT, 1 hit
SMARTiView protein in SMART
SM00119 HECTc, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50237 HECT, 1 hit

Sequencei

Sequence statusi: Complete.

P33202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSENNSHNLD EHESHSENSD YMMDTQVEDD YDEDGHVQGE YSYYPDEDED
60 70 80 90 100
EHMLSSVGSF EADDGEDDDN DYHHEDDSGL LYGYHRTQNG SDEDRNEEED
110 120 130 140 150
GLERSHDNNE FGSNPLHLPD ILETFAQRLE QRRQTSEGLG QHPVGRTLPE
160 170 180 190 200
ILSMIGGRME RSAESSARNE RISKLIENTG NASEDPYIAM ESLKELSENI
210 220 230 240 250
LMMNQMVVDR IIPMETLIGN IAAILSDKIL REELELQMQA CRCMYNLFEV
260 270 280 290 300
CPESISIAVD EHVIPILQGK LVEISYIDLA EQVLETVEYI SRVHGRDILK
310 320 330 340 350
TGQLSIYVQF FDFLTIHAQR KAIAIVSNAC SSIRTDDFKT IVEVLPTLKP
360 370 380 390 400
IFSNATDQPI LTRLVNAMYG ICGALHGVDK FETLFSLDLI ERIVQLVSIQ
410 420 430 440 450
DTPLENKLKC LDILTVLAMS SDVLSRELRE KTDIVDMATR SFQHYSKSPN
460 470 480 490 500
AGLHETLIYV PNSLLISISR FIVVLFPPED ERILSADKYT GNSDRGVISN
510 520 530 540 550
QEKFDSLVQC LIPILVEIYT NAADFDVRRY VLIALLRVVS CINNSTAKAI
560 570 580 590 600
NDQLIKLIGS ILAQKETASN ANGTYSSEAG TLLVGGLSLL DLICKKFSEL
610 620 630 640 650
FFPSIKREGI FDLVKDLSVD FNNIDLKEDG NENISLSDEE GDLHSSIEEC
660 670 680 690 700
DEGDEEYDYE FTDMEIPDSV KPKKISIHIF RTLSLAYIKN KGVNLVNRVL
710 720 730 740 750
SQMNVEQEAI TEELHQIEGV VSILENPSTP DKTEEDWKGI WSVLKKCIFH
760 770 780 790 800
EDFDVSGFEF TSTGLASSIT KRITSSTVSH FILAKSFLEV FEDCIDRFLE
810 820 830 840 850
ILQSALTRLE NFSIVDCGLH DGGGVSSLAK EIKIKLVYDG DASKDNIGTD
860 870 880 890 900
LSSTIVSVHC IASFTSLNEF LRHRMVRMRF LNSLIPNLTS SSTEADREEE
910 920 930 940 950
ENCLDHMRKK NFDFFYDNEK VDMESTVFGV IFNTFVRRNR DLKTLWDDTH
960 970 980 990 1000
TIKFCKSLEG NNRESEAAEE ANEGKKLRDF YKKREFAQVD TGSSADILTL
1010 1020 1030 1040 1050
LDFLHSCGVK SDSFINSKLS AKLARQLDEP LVVASGALPD WSLFLTRRFP
1060 1070 1080 1090 1100
FLFPFDTRML FLQCTSFGYG RLIQLWKNKS KGSKDLRNDE ALQQLGRITR
1110 1120 1130 1140 1150
RKLRISRKTI FATGLKILSK YGSSPDVLEI EYQEEAGTGL GPTLEFYSVV
1160 1170 1180 1190 1200
SKYFARKSLN MWRCNSYSYR SEMDVDTTDD YITTLLFPEP LNPFSNNEKV
1210 1220 1230 1240 1250
IELFGYLGTF VARSLLDNRI LDFRFSKVFF ELLHRMSTPN VTTVPSDVET
1260 1270 1280 1290 1300
CLLMIELVDP LLAKSLKYIV ANKDDNMTLE SLSLTFTVPG NDDIELIPGG
1310 1320 1330 1340 1350
CNKSLNSSNV EEYIHGVIDQ ILGKGIEKQL KAFIEGFSKV FSYERMLILF
1360 1370 1380 1390 1400
PDELVDIFGR VEEDWSMATL YTNLNAEHGY TMDSSIIHDF ISIISAFGKH
1410 1420 1430 1440 1450
ERRLFLQFLT GSPKLPIGGF KSLNPKFTVV LKHAEDGLTA DEYLPSVMTC
1460 1470 1480
ANYLKLPKYT SKDIMRSRLC QAIEEGAGAF LLS
Length:1,483
Mass (Da):167,843
Last modified:February 1, 1994 - v1
Checksum:iA9B8B9D812C6216F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53418 Genomic DNA Translation: AAB24903.1
Z28010 Genomic DNA Translation: CAA81845.1
BK006944 Genomic DNA Translation: DAA09146.1
PIRiS30015
RefSeqiNP_012915.3, NM_001179576.3

Genome annotation databases

EnsemblFungiiYKL010C; YKL010C; YKL010C
GeneIDi853859
KEGGisce:YKL010C

Similar proteinsi

Entry informationi

Entry nameiUFD4_YEAST
AccessioniPrimary (citable) accession number: P33202
Secondary accession number(s): D6VXS6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health