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Protein

Ubiquitin fusion degradation protein 4

Gene

UFD4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1450 – 14501Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • free ubiquitin chain polymerization Source: SGD
  • negative regulation of telomerase activity Source: SGD
  • protein monoubiquitination Source: SGD
  • protein polyubiquitination Source: SGD
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31819-MONOMER.
ReactomeiR-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin fusion degradation protein 4
Short name:
UB fusion protein 4
Gene namesi
Name:UFD4
Ordered Locus Names:YKL010C
ORF Names:YKL162
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL010C.
SGDiS000001493. UFD4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14831483Ubiquitin fusion degradation protein 4PRO_0000194998Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei87 – 871PhosphothreonineCombined sources
Cross-linki349 – 349Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP33202.

PTM databases

iPTMnetiP33202.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RPT6Q019393EBI-20010,EBI-13914

Protein-protein interaction databases

BioGridi34122. 64 interactions.
DIPiDIP-4873N.
IntActiP33202. 25 interactions.
MINTiMINT-542956.

Structurei

3D structure databases

ProteinModelPortaliP33202.
SMRiP33202. Positions 1128-1478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1376 – 1483108HECTPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1007 – 108175K-boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi898 – 9014Poly-Glu

Sequence similaritiesi

Belongs to the UPL family. K-HECT subfamily.Curated
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063470.
HOGENOMiHOG000142004.
InParanoidiP33202.
KOiK10590.
OMAiEVMSEFD.
OrthoDBiEOG092C09RX.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000569. HECT_dom.
[Graphical view]
PfamiPF00632. HECT. 1 hit.
[Graphical view]
SMARTiSM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSENNSHNLD EHESHSENSD YMMDTQVEDD YDEDGHVQGE YSYYPDEDED
60 70 80 90 100
EHMLSSVGSF EADDGEDDDN DYHHEDDSGL LYGYHRTQNG SDEDRNEEED
110 120 130 140 150
GLERSHDNNE FGSNPLHLPD ILETFAQRLE QRRQTSEGLG QHPVGRTLPE
160 170 180 190 200
ILSMIGGRME RSAESSARNE RISKLIENTG NASEDPYIAM ESLKELSENI
210 220 230 240 250
LMMNQMVVDR IIPMETLIGN IAAILSDKIL REELELQMQA CRCMYNLFEV
260 270 280 290 300
CPESISIAVD EHVIPILQGK LVEISYIDLA EQVLETVEYI SRVHGRDILK
310 320 330 340 350
TGQLSIYVQF FDFLTIHAQR KAIAIVSNAC SSIRTDDFKT IVEVLPTLKP
360 370 380 390 400
IFSNATDQPI LTRLVNAMYG ICGALHGVDK FETLFSLDLI ERIVQLVSIQ
410 420 430 440 450
DTPLENKLKC LDILTVLAMS SDVLSRELRE KTDIVDMATR SFQHYSKSPN
460 470 480 490 500
AGLHETLIYV PNSLLISISR FIVVLFPPED ERILSADKYT GNSDRGVISN
510 520 530 540 550
QEKFDSLVQC LIPILVEIYT NAADFDVRRY VLIALLRVVS CINNSTAKAI
560 570 580 590 600
NDQLIKLIGS ILAQKETASN ANGTYSSEAG TLLVGGLSLL DLICKKFSEL
610 620 630 640 650
FFPSIKREGI FDLVKDLSVD FNNIDLKEDG NENISLSDEE GDLHSSIEEC
660 670 680 690 700
DEGDEEYDYE FTDMEIPDSV KPKKISIHIF RTLSLAYIKN KGVNLVNRVL
710 720 730 740 750
SQMNVEQEAI TEELHQIEGV VSILENPSTP DKTEEDWKGI WSVLKKCIFH
760 770 780 790 800
EDFDVSGFEF TSTGLASSIT KRITSSTVSH FILAKSFLEV FEDCIDRFLE
810 820 830 840 850
ILQSALTRLE NFSIVDCGLH DGGGVSSLAK EIKIKLVYDG DASKDNIGTD
860 870 880 890 900
LSSTIVSVHC IASFTSLNEF LRHRMVRMRF LNSLIPNLTS SSTEADREEE
910 920 930 940 950
ENCLDHMRKK NFDFFYDNEK VDMESTVFGV IFNTFVRRNR DLKTLWDDTH
960 970 980 990 1000
TIKFCKSLEG NNRESEAAEE ANEGKKLRDF YKKREFAQVD TGSSADILTL
1010 1020 1030 1040 1050
LDFLHSCGVK SDSFINSKLS AKLARQLDEP LVVASGALPD WSLFLTRRFP
1060 1070 1080 1090 1100
FLFPFDTRML FLQCTSFGYG RLIQLWKNKS KGSKDLRNDE ALQQLGRITR
1110 1120 1130 1140 1150
RKLRISRKTI FATGLKILSK YGSSPDVLEI EYQEEAGTGL GPTLEFYSVV
1160 1170 1180 1190 1200
SKYFARKSLN MWRCNSYSYR SEMDVDTTDD YITTLLFPEP LNPFSNNEKV
1210 1220 1230 1240 1250
IELFGYLGTF VARSLLDNRI LDFRFSKVFF ELLHRMSTPN VTTVPSDVET
1260 1270 1280 1290 1300
CLLMIELVDP LLAKSLKYIV ANKDDNMTLE SLSLTFTVPG NDDIELIPGG
1310 1320 1330 1340 1350
CNKSLNSSNV EEYIHGVIDQ ILGKGIEKQL KAFIEGFSKV FSYERMLILF
1360 1370 1380 1390 1400
PDELVDIFGR VEEDWSMATL YTNLNAEHGY TMDSSIIHDF ISIISAFGKH
1410 1420 1430 1440 1450
ERRLFLQFLT GSPKLPIGGF KSLNPKFTVV LKHAEDGLTA DEYLPSVMTC
1460 1470 1480
ANYLKLPKYT SKDIMRSRLC QAIEEGAGAF LLS
Length:1,483
Mass (Da):167,843
Last modified:February 1, 1994 - v1
Checksum:iA9B8B9D812C6216F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53418 Genomic DNA. Translation: AAB24903.1.
Z28010 Genomic DNA. Translation: CAA81845.1.
BK006944 Genomic DNA. Translation: DAA09146.1.
PIRiS30015.
RefSeqiNP_012915.3. NM_001179576.3.

Genome annotation databases

EnsemblFungiiYKL010C; YKL010C; YKL010C.
GeneIDi853859.
KEGGisce:YKL010C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53418 Genomic DNA. Translation: AAB24903.1.
Z28010 Genomic DNA. Translation: CAA81845.1.
BK006944 Genomic DNA. Translation: DAA09146.1.
PIRiS30015.
RefSeqiNP_012915.3. NM_001179576.3.

3D structure databases

ProteinModelPortaliP33202.
SMRiP33202. Positions 1128-1478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34122. 64 interactions.
DIPiDIP-4873N.
IntActiP33202. 25 interactions.
MINTiMINT-542956.

PTM databases

iPTMnetiP33202.

Proteomic databases

MaxQBiP33202.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL010C; YKL010C; YKL010C.
GeneIDi853859.
KEGGisce:YKL010C.

Organism-specific databases

EuPathDBiFungiDB:YKL010C.
SGDiS000001493. UFD4.

Phylogenomic databases

GeneTreeiENSGT00530000063470.
HOGENOMiHOG000142004.
InParanoidiP33202.
KOiK10590.
OMAiEVMSEFD.
OrthoDBiEOG092C09RX.

Enzyme and pathway databases

BioCyciYEAST:G3O-31819-MONOMER.
ReactomeiR-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiP33202.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000569. HECT_dom.
[Graphical view]
PfamiPF00632. HECT. 1 hit.
[Graphical view]
SMARTiSM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUFD4_YEAST
AccessioniPrimary (citable) accession number: P33202
Secondary accession number(s): D6VXS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

A cysteine residue is required for ubiquitin-thioester formation.
Present with 7380 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.