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Protein

Ribosome assembly factor MRT4

Gene

MRT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.4 Publications

GO - Molecular functioni

GO - Biological processi

  • nuclear-transcribed mRNA catabolic process Source: SGD
  • ribosomal large subunit assembly Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
  • rRNA processing Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-31818-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome assembly factor MRT41 Publication
Alternative name(s):
mRNA turnover protein 41 Publication
Gene namesi
Name:MRT41 Publication
Ordered Locus Names:YKL009WImported
ORF Names:YKL160
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XI

Organism-specific databases

CYGDiYKL009w.
EuPathDBiFungiDB:YKL009W.
SGDiS000001492. MRT4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleolus Source: SGD
  • nucleoplasm Source: SGD
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi68 – 681G → D: Bypasses the requirement for phosphatase YVH1 for the release of MRT4. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236Ribosome assembly factor MRT4PRO_0000154815Add
BLAST

Proteomic databases

MaxQBiP33201.
PaxDbiP33201.
PeptideAtlasiP33201.

Interactioni

Subunit structurei

Associates with the pre-60S ribosomal particle.4 Publications

Protein-protein interaction databases

BioGridi34123. 229 interactions.
DIPiDIP-5283N.
IntActiP33201. 19 interactions.
MINTiMINT-8285311.
STRINGi4932.YKL009W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V7Felectron microscopy8.70n1-236[»]
ProteinModelPortaliP33201.
SMRiP33201. Positions 5-228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L10P family.Curated

Phylogenomic databases

eggNOGiCOG0244.
GeneTreeiENSGT00390000006238.
HOGENOMiHOG000177263.
InParanoidiP33201.
KOiK14815.
OMAiDIRTAWK.
OrthoDBiEOG7RJQ36.

Family and domain databases

InterProiIPR001790. Ribosomal_L10P.
[Graphical view]
PfamiPF00466. Ribosomal_L10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRSKRSKLV TLAQTDKKGR ENKERIFDEV REALDTYRYV WVLHLDDVRT
60 70 80 90 100
PVLQEIRTSW AGSKLIMGKR KVLQKALGEK REEEYKENLY QLSKLCSGVT
110 120 130 140 150
GLLFTDEDVN TVKEYFKSYV RSDYSRPNTK APLTFTIPEG IVYSRGGQIP
160 170 180 190 200
AEEDVPMIHS LEPTMRNKFE IPTKIKAGKI TIDSPYLVCT EGEKLDVRQA
210 220 230
LILKQFGIAA SEFKVKVSAY YDNDSSTVES TNINME
Length:236
Mass (Da):27,058
Last modified:February 1, 1994 - v1
Checksum:i9FCFDEE9F009F427
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53418 Genomic DNA. Translation: AAB24904.1.
X61398 Genomic DNA. No translation available.
Z28009 Genomic DNA. Translation: CAA81844.1.
S59773 Genomic DNA. Translation: AAC60552.1.
BK006944 Genomic DNA. Translation: DAA09147.1.
PIRiS30013.
RefSeqiNP_012916.1. NM_001179575.1.

Genome annotation databases

EnsemblFungiiYKL009W; YKL009W; YKL009W.
GeneIDi853860.
KEGGisce:YKL009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53418 Genomic DNA. Translation: AAB24904.1.
X61398 Genomic DNA. No translation available.
Z28009 Genomic DNA. Translation: CAA81844.1.
S59773 Genomic DNA. Translation: AAC60552.1.
BK006944 Genomic DNA. Translation: DAA09147.1.
PIRiS30013.
RefSeqiNP_012916.1. NM_001179575.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V7Felectron microscopy8.70n1-236[»]
ProteinModelPortaliP33201.
SMRiP33201. Positions 5-228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34123. 229 interactions.
DIPiDIP-5283N.
IntActiP33201. 19 interactions.
MINTiMINT-8285311.
STRINGi4932.YKL009W.

Proteomic databases

MaxQBiP33201.
PaxDbiP33201.
PeptideAtlasiP33201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL009W; YKL009W; YKL009W.
GeneIDi853860.
KEGGisce:YKL009W.

Organism-specific databases

CYGDiYKL009w.
EuPathDBiFungiDB:YKL009W.
SGDiS000001492. MRT4.

Phylogenomic databases

eggNOGiCOG0244.
GeneTreeiENSGT00390000006238.
HOGENOMiHOG000177263.
InParanoidiP33201.
KOiK14815.
OMAiDIRTAWK.
OrthoDBiEOG7RJQ36.

Enzyme and pathway databases

BioCyciYEAST:G3O-31818-MONOMER.

Miscellaneous databases

NextBioi975109.
PROiP33201.

Family and domain databases

InterProiIPR001790. Ribosomal_L10P.
[Graphical view]
PfamiPF00466. Ribosomal_L10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 9.3 kb segment located on the left arm of the yeast chromosome XI reveals five open reading frames including the CCE1 gene and putative products related to MYO2 and to the ribosomal protein L10."
    Pascolo S., Ghazvini M., Boyer J., Colleaux L., Thierry A., Dujon B.
    Yeast 8:987-995(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Sequence of a 7.8 kb segment on the left arm of yeast chromosome XI reveals four open reading frames, including the CAP1 gene, an intron-containing gene and a gene encoding a homolog to the mammalian UOG-1 gene."
    Boyer J., Pascolo S., Richard G.-F., Dujon B.
    Yeast 9:279-287(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 190-236.
  5. "Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover."
    Zuk D., Belk J.P., Jacobson A.
    Genetics 153:35-47(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME, INVOLVEMENT IN MRNA TURNOVER.
  6. "Composition and functional characterization of yeast 66S ribosome assembly intermediates."
    Harnpicharnchai P., Jakovljevic J., Horsey E., Miles T., Roman J., Rout M., Meagher D., Imai B., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Woolford J.L. Jr.
    Mol. Cell 8:505-515(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INVOLVEMENT IN RIBOSOME BIOGENESIS.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0."
    Lo K.Y., Li Z., Wang F., Marcotte E.M., Johnson A.W.
    J. Cell Biol. 186:849-862(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF GLY-68.
  10. "The amino terminal domain from Mrt4 protein can functionally replace the RNA binding domain of the ribosomal P0 protein."
    Rodriguez-Mateos M., Abia D., Garcia-Gomez J.J., Morreale A., de la Cruz J., Santos C., Remacha M., Ballesta J.P.
    Nucleic Acids Res. 37:3514-3521(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  11. "Role and dynamics of the ribosomal protein P0 and its related trans-acting factor Mrt4 during ribosome assembly in Saccharomyces cerevisiae."
    Rodriguez-Mateos M., Garcia-Gomez J.J., Francisco-Velilla R., Remacha M., de la Cruz J., Ballesta J.P.
    Nucleic Acids Res. 37:7519-7532(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  12. "60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle."
    Leidig C., Thoms M., Holdermann I., Bradatsch B., Berninghausen O., Bange G., Sinning I., Hurt E., Beckmann R.
    Nat. Commun. 5:3491-3491(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.70 ANGSTROMS).

Entry informationi

Entry nameiMRT4_YEAST
AccessioniPrimary (citable) accession number: P33201
Secondary accession number(s): D6VXS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 24, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 18200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.