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P33201

- MRT4_YEAST

UniProt

P33201 - MRT4_YEAST

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Protein

Ribosome assembly factor MRT4

Gene

MRT4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.4 Publications

GO - Molecular functioni

  1. large ribosomal subunit rRNA binding Source: SGD

GO - Biological processi

  1. nuclear-transcribed mRNA catabolic process Source: SGD
  2. ribosomal large subunit assembly Source: SGD
  3. ribosomal large subunit biogenesis Source: SGD
  4. rRNA processing Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-31818-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome assembly factor MRT41 Publication
Alternative name(s):
mRNA turnover protein 41 Publication
Gene namesi
Name:MRT41 Publication
Ordered Locus Names:YKL009WImported
ORF Names:YKL160
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL009w.
SGDiS000001492. MRT4.

Subcellular locationi

Nucleusnucleolus 2 Publications. Cytoplasm 1 Publication
Note: Shutles between the nucleus and the cytoplasm.1 Publication

GO - Cellular componenti

  1. nucleolus Source: SGD
  2. nucleoplasm Source: SGD
  3. preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi68 – 681G → D: Bypasses the requirement for phosphatase YVH1 for the release of MRT4. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236Ribosome assembly factor MRT4PRO_0000154815Add
BLAST

Proteomic databases

MaxQBiP33201.
PaxDbiP33201.
PeptideAtlasiP33201.

Expressioni

Gene expression databases

GenevestigatoriP33201.

Interactioni

Subunit structurei

Associates with the pre-60S ribosomal particle.4 Publications

Protein-protein interaction databases

BioGridi34123. 228 interactions.
DIPiDIP-5283N.
IntActiP33201. 19 interactions.
MINTiMINT-8285311.
STRINGi4932.YKL009W.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J65electron microscopy8.70n1-236[»]
ProteinModelPortaliP33201.
SMRiP33201. Positions 5-228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L10P family.Curated

Phylogenomic databases

eggNOGiCOG0244.
GeneTreeiENSGT00390000006238.
HOGENOMiHOG000177263.
InParanoidiP33201.
KOiK14815.
OMAiKTKVMAK.
OrthoDBiEOG7RJQ36.

Family and domain databases

InterProiIPR001790. Ribosomal_L10/acidic_P0.
[Graphical view]
PfamiPF00466. Ribosomal_L10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33201-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPRSKRSKLV TLAQTDKKGR ENKERIFDEV REALDTYRYV WVLHLDDVRT
60 70 80 90 100
PVLQEIRTSW AGSKLIMGKR KVLQKALGEK REEEYKENLY QLSKLCSGVT
110 120 130 140 150
GLLFTDEDVN TVKEYFKSYV RSDYSRPNTK APLTFTIPEG IVYSRGGQIP
160 170 180 190 200
AEEDVPMIHS LEPTMRNKFE IPTKIKAGKI TIDSPYLVCT EGEKLDVRQA
210 220 230
LILKQFGIAA SEFKVKVSAY YDNDSSTVES TNINME
Length:236
Mass (Da):27,058
Last modified:February 1, 1994 - v1
Checksum:i9FCFDEE9F009F427
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S53418 Genomic DNA. Translation: AAB24904.1.
X61398 Genomic DNA. No translation available.
Z28009 Genomic DNA. Translation: CAA81844.1.
S59773 Genomic DNA. Translation: AAC60552.1.
BK006944 Genomic DNA. Translation: DAA09147.1.
PIRiS30013.
RefSeqiNP_012916.1. NM_001179575.1.

Genome annotation databases

EnsemblFungiiYKL009W; YKL009W; YKL009W.
GeneIDi853860.
KEGGisce:YKL009W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S53418 Genomic DNA. Translation: AAB24904.1 .
X61398 Genomic DNA. No translation available.
Z28009 Genomic DNA. Translation: CAA81844.1 .
S59773 Genomic DNA. Translation: AAC60552.1 .
BK006944 Genomic DNA. Translation: DAA09147.1 .
PIRi S30013.
RefSeqi NP_012916.1. NM_001179575.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3J65 electron microscopy 8.70 n 1-236 [» ]
ProteinModelPortali P33201.
SMRi P33201. Positions 5-228.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34123. 228 interactions.
DIPi DIP-5283N.
IntActi P33201. 19 interactions.
MINTi MINT-8285311.
STRINGi 4932.YKL009W.

Proteomic databases

MaxQBi P33201.
PaxDbi P33201.
PeptideAtlasi P33201.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL009W ; YKL009W ; YKL009W .
GeneIDi 853860.
KEGGi sce:YKL009W.

Organism-specific databases

CYGDi YKL009w.
SGDi S000001492. MRT4.

Phylogenomic databases

eggNOGi COG0244.
GeneTreei ENSGT00390000006238.
HOGENOMi HOG000177263.
InParanoidi P33201.
KOi K14815.
OMAi KTKVMAK.
OrthoDBi EOG7RJQ36.

Enzyme and pathway databases

BioCyci YEAST:G3O-31818-MONOMER.

Miscellaneous databases

NextBioi 975109.
PROi P33201.

Gene expression databases

Genevestigatori P33201.

Family and domain databases

InterProi IPR001790. Ribosomal_L10/acidic_P0.
[Graphical view ]
Pfami PF00466. Ribosomal_L10. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 9.3 kb segment located on the left arm of the yeast chromosome XI reveals five open reading frames including the CCE1 gene and putative products related to MYO2 and to the ribosomal protein L10."
    Pascolo S., Ghazvini M., Boyer J., Colleaux L., Thierry A., Dujon B.
    Yeast 8:987-995(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Sequence of a 7.8 kb segment on the left arm of yeast chromosome XI reveals four open reading frames, including the CAP1 gene, an intron-containing gene and a gene encoding a homolog to the mammalian UOG-1 gene."
    Boyer J., Pascolo S., Richard G.-F., Dujon B.
    Yeast 9:279-287(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 190-236.
  5. "Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover."
    Zuk D., Belk J.P., Jacobson A.
    Genetics 153:35-47(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME, INVOLVEMENT IN MRNA TURNOVER.
  6. "Composition and functional characterization of yeast 66S ribosome assembly intermediates."
    Harnpicharnchai P., Jakovljevic J., Horsey E., Miles T., Roman J., Rout M., Meagher D., Imai B., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Woolford J.L. Jr.
    Mol. Cell 8:505-515(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INVOLVEMENT IN RIBOSOME BIOGENESIS.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0."
    Lo K.Y., Li Z., Wang F., Marcotte E.M., Johnson A.W.
    J. Cell Biol. 186:849-862(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF GLY-68.
  10. "The amino terminal domain from Mrt4 protein can functionally replace the RNA binding domain of the ribosomal P0 protein."
    Rodriguez-Mateos M., Abia D., Garcia-Gomez J.J., Morreale A., de la Cruz J., Santos C., Remacha M., Ballesta J.P.
    Nucleic Acids Res. 37:3514-3521(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  11. "Role and dynamics of the ribosomal protein P0 and its related trans-acting factor Mrt4 during ribosome assembly in Saccharomyces cerevisiae."
    Rodriguez-Mateos M., Garcia-Gomez J.J., Francisco-Velilla R., Remacha M., de la Cruz J., Ballesta J.P.
    Nucleic Acids Res. 37:7519-7532(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  12. "60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle."
    Leidig C., Thoms M., Holdermann I., Bradatsch B., Berninghausen O., Bange G., Sinning I., Hurt E., Beckmann R.
    Nat. Commun. 5:3491-3491(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.70 ANGSTROMS).

Entry informationi

Entry nameiMRT4_YEAST
AccessioniPrimary (citable) accession number: P33201
Secondary accession number(s): D6VXS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 18200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3