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P33197

- IDH_THET8

UniProt

P33197 - IDH_THET8

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Protein

Isocitrate dehydrogenase [NADP]

Gene

icd

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Binds 1 magnesium or manganese ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041SubstrateBy similarity
Binding sitei114 – 1141SubstrateBy similarity
Binding sitei137 – 1371SubstrateBy similarity
Sitei144 – 1441Critical for catalysisBy similarity
Sitei191 – 1911Critical for catalysisBy similarity
Metal bindingi224 – 2241Magnesium or manganeseBy similarity
Binding sitei224 – 2241SubstrateBy similarity
Metal bindingi248 – 2481Magnesium or manganeseBy similarity
Metal bindingi252 – 2521Magnesium or manganeseBy similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1572-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:icd
Ordered Locus Names:TTHA1535
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Isocitrate dehydrogenase [NADP]PRO_0000083571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei98 – 981PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

PTM databases

PhosSiteiP12101814.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi300852.TTHA1535.

Structurei

Secondary structure

1
496
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53
Beta strandi11 – 144
Beta strandi20 – 256
Helixi31 – 4414
Beta strandi49 – 535
Helixi58 – 614
Turni62 – 643
Helixi71 – 8010
Beta strandi82 – 854
Beta strandi92 – 965
Helixi99 – 1068
Beta strandi111 – 1177
Turni125 – 1284
Beta strandi132 – 1387
Beta strandi140 – 1423
Helixi143 – 1453
Beta strandi148 – 1525
Beta strandi155 – 1639
Helixi164 – 18017
Beta strandi185 – 1906
Turni192 – 1943
Helixi198 – 21013
Beta strandi216 – 2227
Helixi223 – 23210
Helixi234 – 2363
Beta strandi238 – 2425
Helixi244 – 25512
Turni256 – 2594
Helixi261 – 2633
Beta strandi265 – 2695
Beta strandi274 – 2807
Turni284 – 2885
Helixi295 – 30713
Helixi311 – 32717
Helixi333 – 3364
Turni338 – 3403
Helixi344 – 35310
Turni354 – 3563
Beta strandi360 – 3623
Helixi375 – 3773
Beta strandi387 – 39711
Helixi402 – 41312
Beta strandi416 – 42510
Beta strandi428 – 4325
Beta strandi442 – 4509
Beta strandi452 – 4543
Helixi458 – 46912
Beta strandi472 – 48312
Beta strandi486 – 4894

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D1CX-ray1.80A/B1-496[»]
ProteinModelPortaliP33197.
SMRiP33197. Positions 2-496.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33197.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0473.
HOGENOMiHOG000021113.
KOiK00031.
OMAiTEYTEAI.
OrthoDBiEOG65XN31.
PhylomeDBiP33197.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33197 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLITTETGK KMHVLEDGRK LITVIPGDGI GPECVEATLK VLEAAKAPLA
60 70 80 90 100
YEVREAGASV FRRGIASGVP QETIESIRKT RVVLKGPLET PVGYGEKSAN
110 120 130 140 150
VTLRKLFETY ANVRPVREFP NVPTPYAGRG IDLVVVRENV EDLYAGIEHM
160 170 180 190 200
QTPSVAQTLK LISWKGSEKI VRFAFELARA EGRKKVHCAT KSNIMKLAEG
210 220 230 240 250
TLKRAFEQVA QEYPDIEAVH IIVDNAAHQL VKRPEQFEVI VTTNMNGDIL
260 270 280 290 300
SDLTSGLIGG LGFAPSANIG NEVAIFEAVH GSAPKYAGKN VINPTAVLLS
310 320 330 340 350
AVMMLRYLEE FATADLIENA LLYTLEEGRV LTGDVVGYDR GAKTTEYTEA
360 370 380 390 400
IIQNLGKTPR KTQVRGYKPF RLPQVDGAIA PIVPRSRRVV GVDVFVETNL
410 420 430 440 450
LPEALGKALE DLAAGTPFRL KMISNRGTQV YPPTGGLTDL VDHYRCRFLY
460 470 480 490
TGEGEAKDPE ILDLVSRVAS RFRWMHLEKL QEFDGEPGFT KAQGED
Length:496
Mass (Da):54,439
Last modified:March 29, 2005 - v2
Checksum:iBF544C3F772E1AE9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti201 – 2022TL → P in AAA27492. (PubMed:1539996)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M94317 Genomic DNA. Translation: AAA27492.1.
AP008226 Genomic DNA. Translation: BAD71358.1.
RefSeqiYP_144801.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71358; BAD71358; BAD71358.
GeneIDi3168917.
KEGGittj:TTHA1535.
PATRICi23958029. VBITheThe93045_1509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M94317 Genomic DNA. Translation: AAA27492.1 .
AP008226 Genomic DNA. Translation: BAD71358.1 .
RefSeqi YP_144801.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2D1C X-ray 1.80 A/B 1-496 [» ]
ProteinModelPortali P33197.
SMRi P33197. Positions 2-496.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 300852.TTHA1535.

PTM databases

PhosSitei P12101814.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD71358 ; BAD71358 ; BAD71358 .
GeneIDi 3168917.
KEGGi ttj:TTHA1535.
PATRICi 23958029. VBITheThe93045_1509.

Phylogenomic databases

eggNOGi COG0473.
HOGENOMi HOG000021113.
KOi K00031.
OMAi TEYTEAI.
OrthoDBi EOG65XN31.
PhylomeDBi P33197.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-1572-MONOMER.

Miscellaneous databases

EvolutionaryTracei P33197.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11835. PTHR11835. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the isocitrate dehydrogenase gene of an extreme thermophile, Thermus thermophilus HB8."
    Miyazaki K., Eguchi H., Yamagishi A., Wakagi T., Oshima T.
    Appl. Environ. Microbiol. 58:93-98(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiIDH_THET8
AccessioniPrimary (citable) accession number: P33197
Secondary accession number(s): Q5SI45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 29, 2005
Last modified: October 29, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3