Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isocitrate dehydrogenase [NADP]

Gene

icd

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104SubstrateBy similarity1
Binding sitei114SubstrateBy similarity1
Binding sitei137SubstrateBy similarity1
Sitei144Critical for catalysisBy similarity1
Sitei191Critical for catalysisBy similarity1
Metal bindingi224Magnesium or manganeseBy similarity1
Binding sitei224SubstrateBy similarity1
Metal bindingi248Magnesium or manganeseBy similarity1
Metal bindingi252Magnesium or manganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.1.1.42. 2305.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene namesi
Name:icd
Ordered Locus Names:TTHA1535
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835711 – 496Isocitrate dehydrogenase [NADP]Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei98PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi300852.TTHA1535.

Structurei

Secondary structure

1496
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Beta strandi11 – 14Combined sources4
Beta strandi20 – 25Combined sources6
Helixi31 – 44Combined sources14
Beta strandi49 – 53Combined sources5
Helixi58 – 61Combined sources4
Turni62 – 64Combined sources3
Helixi71 – 80Combined sources10
Beta strandi82 – 85Combined sources4
Beta strandi92 – 96Combined sources5
Helixi99 – 106Combined sources8
Beta strandi111 – 117Combined sources7
Turni125 – 128Combined sources4
Beta strandi132 – 138Combined sources7
Beta strandi140 – 142Combined sources3
Helixi143 – 145Combined sources3
Beta strandi148 – 152Combined sources5
Beta strandi155 – 163Combined sources9
Helixi164 – 180Combined sources17
Beta strandi185 – 190Combined sources6
Turni192 – 194Combined sources3
Helixi198 – 210Combined sources13
Beta strandi216 – 222Combined sources7
Helixi223 – 232Combined sources10
Helixi234 – 236Combined sources3
Beta strandi238 – 242Combined sources5
Helixi244 – 255Combined sources12
Turni256 – 259Combined sources4
Helixi261 – 263Combined sources3
Beta strandi265 – 269Combined sources5
Beta strandi274 – 280Combined sources7
Turni284 – 288Combined sources5
Helixi295 – 307Combined sources13
Helixi311 – 327Combined sources17
Helixi333 – 336Combined sources4
Turni338 – 340Combined sources3
Helixi344 – 353Combined sources10
Turni354 – 356Combined sources3
Beta strandi360 – 362Combined sources3
Helixi375 – 377Combined sources3
Beta strandi387 – 397Combined sources11
Helixi402 – 413Combined sources12
Beta strandi416 – 425Combined sources10
Beta strandi428 – 432Combined sources5
Beta strandi442 – 450Combined sources9
Beta strandi452 – 454Combined sources3
Helixi458 – 469Combined sources12
Beta strandi472 – 483Combined sources12
Beta strandi486 – 489Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1CX-ray1.80A/B1-496[»]
ProteinModelPortaliP33197.
SMRiP33197.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33197.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C0C. Bacteria.
COG0473. LUCA.
HOGENOMiHOG000021113.
KOiK00031.
OMAiTNLPHET.
PhylomeDBiP33197.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLITTETGK KMHVLEDGRK LITVIPGDGI GPECVEATLK VLEAAKAPLA
60 70 80 90 100
YEVREAGASV FRRGIASGVP QETIESIRKT RVVLKGPLET PVGYGEKSAN
110 120 130 140 150
VTLRKLFETY ANVRPVREFP NVPTPYAGRG IDLVVVRENV EDLYAGIEHM
160 170 180 190 200
QTPSVAQTLK LISWKGSEKI VRFAFELARA EGRKKVHCAT KSNIMKLAEG
210 220 230 240 250
TLKRAFEQVA QEYPDIEAVH IIVDNAAHQL VKRPEQFEVI VTTNMNGDIL
260 270 280 290 300
SDLTSGLIGG LGFAPSANIG NEVAIFEAVH GSAPKYAGKN VINPTAVLLS
310 320 330 340 350
AVMMLRYLEE FATADLIENA LLYTLEEGRV LTGDVVGYDR GAKTTEYTEA
360 370 380 390 400
IIQNLGKTPR KTQVRGYKPF RLPQVDGAIA PIVPRSRRVV GVDVFVETNL
410 420 430 440 450
LPEALGKALE DLAAGTPFRL KMISNRGTQV YPPTGGLTDL VDHYRCRFLY
460 470 480 490
TGEGEAKDPE ILDLVSRVAS RFRWMHLEKL QEFDGEPGFT KAQGED
Length:496
Mass (Da):54,439
Last modified:March 29, 2005 - v2
Checksum:iBF544C3F772E1AE9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201 – 202TL → P in AAA27492 (PubMed:1539996).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94317 Genomic DNA. Translation: AAA27492.1.
AP008226 Genomic DNA. Translation: BAD71358.1.
RefSeqiWP_011228743.1. NC_006461.1.
YP_144801.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71358; BAD71358; BAD71358.
GeneIDi3168917.
KEGGittj:TTHA1535.
PATRICi23958029. VBITheThe93045_1509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94317 Genomic DNA. Translation: AAA27492.1.
AP008226 Genomic DNA. Translation: BAD71358.1.
RefSeqiWP_011228743.1. NC_006461.1.
YP_144801.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D1CX-ray1.80A/B1-496[»]
ProteinModelPortaliP33197.
SMRiP33197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1535.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71358; BAD71358; BAD71358.
GeneIDi3168917.
KEGGittj:TTHA1535.
PATRICi23958029. VBITheThe93045_1509.

Phylogenomic databases

eggNOGiENOG4105C0C. Bacteria.
COG0473. LUCA.
HOGENOMiHOG000021113.
KOiK00031.
OMAiTNLPHET.
PhylomeDBiP33197.

Enzyme and pathway databases

BRENDAi1.1.1.42. 2305.

Miscellaneous databases

EvolutionaryTraceiP33197.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIDH_THET8
AccessioniPrimary (citable) accession number: P33197
Secondary accession number(s): Q5SI45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.