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P33197

- IDH_THET8

UniProt

P33197 - IDH_THET8

Protein

Isocitrate dehydrogenase [NADP]

Gene

icd

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041SubstrateBy similarity
    Binding sitei114 – 1141SubstrateBy similarity
    Binding sitei137 – 1371SubstrateBy similarity
    Sitei144 – 1441Critical for catalysisBy similarity
    Sitei191 – 1911Critical for catalysisBy similarity
    Metal bindingi224 – 2241Magnesium or manganeseBy similarity
    Binding sitei224 – 2241SubstrateBy similarity
    Metal bindingi248 – 2481Magnesium or manganeseBy similarity
    Metal bindingi252 – 2521Magnesium or manganeseBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-KW
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-1572-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP] (EC:1.1.1.42)
    Short name:
    IDH
    Alternative name(s):
    IDP
    NADP(+)-specific ICDH
    Oxalosuccinate decarboxylase
    Gene namesi
    Name:icd
    Ordered Locus Names:TTHA1535
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 496496Isocitrate dehydrogenase [NADP]PRO_0000083571Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei98 – 981PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    PTM databases

    PhosSiteiP12101814.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi300852.TTHA1535.

    Structurei

    Secondary structure

    1
    496
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 53
    Beta strandi11 – 144
    Beta strandi20 – 256
    Helixi31 – 4414
    Beta strandi49 – 535
    Helixi58 – 614
    Turni62 – 643
    Helixi71 – 8010
    Beta strandi82 – 854
    Beta strandi92 – 965
    Helixi99 – 1068
    Beta strandi111 – 1177
    Turni125 – 1284
    Beta strandi132 – 1387
    Beta strandi140 – 1423
    Helixi143 – 1453
    Beta strandi148 – 1525
    Beta strandi155 – 1639
    Helixi164 – 18017
    Beta strandi185 – 1906
    Turni192 – 1943
    Helixi198 – 21013
    Beta strandi216 – 2227
    Helixi223 – 23210
    Helixi234 – 2363
    Beta strandi238 – 2425
    Helixi244 – 25512
    Turni256 – 2594
    Helixi261 – 2633
    Beta strandi265 – 2695
    Beta strandi274 – 2807
    Turni284 – 2885
    Helixi295 – 30713
    Helixi311 – 32717
    Helixi333 – 3364
    Turni338 – 3403
    Helixi344 – 35310
    Turni354 – 3563
    Beta strandi360 – 3623
    Helixi375 – 3773
    Beta strandi387 – 39711
    Helixi402 – 41312
    Beta strandi416 – 42510
    Beta strandi428 – 4325
    Beta strandi442 – 4509
    Beta strandi452 – 4543
    Helixi458 – 46912
    Beta strandi472 – 48312
    Beta strandi486 – 4894

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2D1CX-ray1.80A/B1-496[»]
    ProteinModelPortaliP33197.
    SMRiP33197. Positions 2-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP33197.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0473.
    HOGENOMiHOG000021113.
    KOiK00031.
    OMAiTEYTEAI.
    OrthoDBiEOG65XN31.
    PhylomeDBiP33197.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P33197-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLITTETGK KMHVLEDGRK LITVIPGDGI GPECVEATLK VLEAAKAPLA    50
    YEVREAGASV FRRGIASGVP QETIESIRKT RVVLKGPLET PVGYGEKSAN 100
    VTLRKLFETY ANVRPVREFP NVPTPYAGRG IDLVVVRENV EDLYAGIEHM 150
    QTPSVAQTLK LISWKGSEKI VRFAFELARA EGRKKVHCAT KSNIMKLAEG 200
    TLKRAFEQVA QEYPDIEAVH IIVDNAAHQL VKRPEQFEVI VTTNMNGDIL 250
    SDLTSGLIGG LGFAPSANIG NEVAIFEAVH GSAPKYAGKN VINPTAVLLS 300
    AVMMLRYLEE FATADLIENA LLYTLEEGRV LTGDVVGYDR GAKTTEYTEA 350
    IIQNLGKTPR KTQVRGYKPF RLPQVDGAIA PIVPRSRRVV GVDVFVETNL 400
    LPEALGKALE DLAAGTPFRL KMISNRGTQV YPPTGGLTDL VDHYRCRFLY 450
    TGEGEAKDPE ILDLVSRVAS RFRWMHLEKL QEFDGEPGFT KAQGED 496
    Length:496
    Mass (Da):54,439
    Last modified:March 29, 2005 - v2
    Checksum:iBF544C3F772E1AE9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti201 – 2022TL → P in AAA27492. (PubMed:1539996)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94317 Genomic DNA. Translation: AAA27492.1.
    AP008226 Genomic DNA. Translation: BAD71358.1.
    RefSeqiWP_011228743.1. NC_006461.1.
    YP_144801.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD71358; BAD71358; BAD71358.
    GeneIDi3168917.
    KEGGittj:TTHA1535.
    PATRICi23958029. VBITheThe93045_1509.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94317 Genomic DNA. Translation: AAA27492.1 .
    AP008226 Genomic DNA. Translation: BAD71358.1 .
    RefSeqi WP_011228743.1. NC_006461.1.
    YP_144801.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2D1C X-ray 1.80 A/B 1-496 [» ]
    ProteinModelPortali P33197.
    SMRi P33197. Positions 2-496.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 300852.TTHA1535.

    PTM databases

    PhosSitei P12101814.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD71358 ; BAD71358 ; BAD71358 .
    GeneIDi 3168917.
    KEGGi ttj:TTHA1535.
    PATRICi 23958029. VBITheThe93045_1509.

    Phylogenomic databases

    eggNOGi COG0473.
    HOGENOMi HOG000021113.
    KOi K00031.
    OMAi TEYTEAI.
    OrthoDBi EOG65XN31.
    PhylomeDBi P33197.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-1572-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P33197.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the isocitrate dehydrogenase gene of an extreme thermophile, Thermus thermophilus HB8."
      Miyazaki K., Eguchi H., Yamagishi A., Wakagi T., Oshima T.
      Appl. Environ. Microbiol. 58:93-98(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.

    Entry informationi

    Entry nameiIDH_THET8
    AccessioniPrimary (citable) accession number: P33197
    Secondary accession number(s): Q5SI45
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3