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P33197 (IDH_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP]

Short name=IDH
EC=1.1.1.42
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
Gene names
Name:icd
Ordered Locus Names:TTHA1535
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Isocitrate dehydrogenase [NADP]
PRO_0000083571

Sites

Metal binding2241Magnesium or manganese By similarity
Metal binding2481Magnesium or manganese By similarity
Metal binding2521Magnesium or manganese By similarity
Binding site1041Substrate By similarity
Binding site1141Substrate By similarity
Binding site1371Substrate By similarity
Binding site2241Substrate By similarity
Site1441Critical for catalysis By similarity
Site1911Critical for catalysis By similarity

Amino acid modifications

Modified residue981Phosphoserine By similarity

Experimental info

Sequence conflict201 – 2022TL → P in AAA27492. Ref.1

Secondary structure

............................................................................................. 496
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P33197 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: BF544C3F772E1AE9

FASTA49654,439
        10         20         30         40         50         60 
MPLITTETGK KMHVLEDGRK LITVIPGDGI GPECVEATLK VLEAAKAPLA YEVREAGASV 

        70         80         90        100        110        120 
FRRGIASGVP QETIESIRKT RVVLKGPLET PVGYGEKSAN VTLRKLFETY ANVRPVREFP 

       130        140        150        160        170        180 
NVPTPYAGRG IDLVVVRENV EDLYAGIEHM QTPSVAQTLK LISWKGSEKI VRFAFELARA 

       190        200        210        220        230        240 
EGRKKVHCAT KSNIMKLAEG TLKRAFEQVA QEYPDIEAVH IIVDNAAHQL VKRPEQFEVI 

       250        260        270        280        290        300 
VTTNMNGDIL SDLTSGLIGG LGFAPSANIG NEVAIFEAVH GSAPKYAGKN VINPTAVLLS 

       310        320        330        340        350        360 
AVMMLRYLEE FATADLIENA LLYTLEEGRV LTGDVVGYDR GAKTTEYTEA IIQNLGKTPR 

       370        380        390        400        410        420 
KTQVRGYKPF RLPQVDGAIA PIVPRSRRVV GVDVFVETNL LPEALGKALE DLAAGTPFRL 

       430        440        450        460        470        480 
KMISNRGTQV YPPTGGLTDL VDHYRCRFLY TGEGEAKDPE ILDLVSRVAS RFRWMHLEKL 

       490 
QEFDGEPGFT KAQGED 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of the isocitrate dehydrogenase gene of an extreme thermophile, Thermus thermophilus HB8."
Miyazaki K., Eguchi H., Yamagishi A., Wakagi T., Oshima T.
Appl. Environ. Microbiol. 58:93-98(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M94317 Genomic DNA. Translation: AAA27492.1.
AP008226 Genomic DNA. Translation: BAD71358.1.
RefSeqYP_144801.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2D1CX-ray1.80A/B1-496[»]
ProteinModelPortalP33197.
SMRP33197. Positions 2-496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA1535.

PTM databases

PhosSiteP12101814.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71358; BAD71358; BAD71358.
GeneID3168917.
KEGGttj:TTHA1535.
PATRIC23958029. VBITheThe93045_1509.

Phylogenomic databases

eggNOGCOG0473.
HOGENOMHOG000021113.
KOK00031.
OMADFVCVRE.
OrthoDBEOG65XN31.
ProtClustDBPRK09222.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1572-MONOMER.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11835. PTHR11835. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP33197.

Entry information

Entry nameIDH_THET8
AccessionPrimary (citable) accession number: P33197
Secondary accession number(s): Q5SI45
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 29, 2005
Last modified: November 13, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references