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P33195 (GCSP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycine dehydrogenase (decarboxylating)

EC=1.4.4.2
Alternative name(s):
Glycine cleavage system P-protein
Glycine decarboxylase
Glycine dehydrogenase (aminomethyl-transferring)
Gene names
Name:gcvP
Ordered Locus Names:b2903, JW2871
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length957 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. HAMAP-Rule MF_00711

Catalytic activity

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2. HAMAP-Rule MF_00711

Cofactor

Pyridoxal phosphate.

Subunit structure

The glycine cleavage system is composed of four proteins: P, T, L and H.

Induction

By glycine. HAMAP-Rule MF_00711

Sequence similarities

Belongs to the GcvP family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-551489,EBI-551489

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 957956Glycine dehydrogenase (decarboxylating) HAMAP-Rule MF_00711
PRO_0000166913

Amino acid modifications

Modified residue7081N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P33195 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9983F4DCDC1095A0

FASTA957104,376
        10         20         30         40         50         60 
MTQTLSQLEN SGAFIERHIG PDAAQQQEML NAVGAQSLNA LTGQIVPKDI QLATPPQVGA 

        70         80         90        100        110        120 
PATEYAALAE LKAIASRNKR FTSYIGMGYT AVQLPPVILR NMLENPGWYT AYTPYQPEVS 

       130        140        150        160        170        180 
QGRLEALLNF QQVTLDLTGL DMASASLLDE ATAAAEAMAM AKRVSKLKNA NRFFVASDVH 

       190        200        210        220        230        240 
PQTLDVVRTR AETFGFEVIV DDAQKVLDHQ DVFGVLLQQV GTTGEIHDYT ALISELKSRK 

       250        260        270        280        290        300 
IVVSVAADIM ALVLLTAPGK QGADIVFGSA QRFGVPMGYG GPHAAFFAAK DEYKRSMPGR 

       310        320        330        340        350        360 
IIGVSKDAAG NTALRMAMQT REQHIRREKA NSNICTSQVL LANIASLYAV YHGPVGLKRI 

       370        380        390        400        410        420 
ANRIHRLTDI LAAGLQQKGL KLRHAHYFDT LCVEVADKAG VLTRAEAAEI NLRSDILNAV 

       430        440        450        460        470        480 
GITLDETTTR ENVMQLFNVL LGDNHGLDID TLDKDVAHDS RSIQPAMLRD DEILTHPVFN 

       490        500        510        520        530        540 
RYHSETEMMR YMHSLERKDL ALNQAMIPLG SCTMKLNAAA EMIPITWPEF AELHPFCPPE 

       550        560        570        580        590        600 
QAEGYQQMIA QLADWLVKLT GYDAVCMQPN SGAQGEYAGL LAIRHYHESR NEGHRDICLI 

       610        620        630        640        650        660 
PASAHGTNPA SAHMAGMQVV VVACDKNGNI DLTDLRAKAE QAGDNLSCIM VTYPSTHGVY 

       670        680        690        700        710        720 
EETIREVCEV VHQFGGQVYL DGANMNAQVG ITSPGFIGAD VSHLNLHKTF CIPHGGGGPG 

       730        740        750        760        770        780 
MGPIGVKAHL APFVPGHSVV QIEGMLTRQG AVSAAPFGSA SILPISWMYI RMMGAEGLKK 

       790        800        810        820        830        840 
ASQVAILNAN YIASRLQDAF PVLYTGRDGR VAHECILDIR PLKEETGISE LDIAKRLIDY 

       850        860        870        880        890        900 
GFHAPTMSFP VAGTLMVEPT ESESKVELDR FIDAMLAIRA EIDQVKAGVW PLEDNPLVNA 

       910        920        930        940        950 
PHIQSELVAE WAHPYSREVA VFPAGVADKY WPTVKRLDDV YGDRNLFCSC VPISEYQ 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the Escherichia coli gcv operon."
Stauffer L.T., Fogarty S.J., Stauffer G.V.
Gene 142:17-22(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: K12.
[2]"Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system."
Okamura-Ikeda K., Ohmura Y., Fujiwara K., Motokawa Y.
Eur. J. Biochem. 216:539-548(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L20872 Genomic DNA. Translation: AAA23867.1.
X73958 Genomic DNA. Translation: CAA52146.1.
U28377 Genomic DNA. Translation: AAA69071.1.
U00096 Genomic DNA. Translation: AAC75941.1.
AP009048 Genomic DNA. Translation: BAE76968.1.
PIRS36834.
RefSeqNP_417379.1. NC_000913.3.
YP_491104.1. NC_007779.1.

3D structure databases

ProteinModelPortalP33195.
SMRP33195. Positions 1-950.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9753N.
IntActP33195. 14 interactions.
MINTMINT-1318484.
STRING511145.b2903.

Proteomic databases

PaxDbP33195.
PRIDEP33195.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75941; AAC75941; b2903.
BAE76968; BAE76968; BAE76968.
GeneID12930714.
947394.
KEGGecj:Y75_p2835.
eco:b2903.
PATRIC32121220. VBIEscCol129921_2998.

Organism-specific databases

EchoBASEEB1758.
EcoGeneEG11810. gcvP.

Phylogenomic databases

eggNOGCOG1003.
HOGENOMHOG000239369.
KOK00281.
OMAQTMVCDL.
OrthoDBEOG6HMXDX.
PhylomeDBP33195.
ProtClustDBPRK05367.

Enzyme and pathway databases

BioCycEcoCyc:GCVP-MONOMER.
ECOL316407:JW2871-MONOMER.
MetaCyc:GCVP-MONOMER.

Gene expression databases

GenevestigatorP33195.

Family and domain databases

Gene3D3.40.640.10. 2 hits.
HAMAPMF_00711. GcvP.
InterProIPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERPTHR11773. PTHR11773. 1 hit.
PfamPF02347. GDC-P. 2 hits.
[Graphical view]
SUPFAMSSF53383. SSF53383. 3 hits.
TIGRFAMsTIGR00461. gcvP. 1 hit.
ProtoNetSearch...

Other

PROP33195.

Entry information

Entry nameGCSP_ECOLI
AccessionPrimary (citable) accession number: P33195
Secondary accession number(s): Q2M9T8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene