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P33195

- GCSP_ECOLI

UniProt

P33195 - GCSP_ECOLI

Protein

Glycine dehydrogenase (decarboxylating)

Gene

gcvP

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.

    Catalytic activityi

    Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2.

    Cofactori

    Pyridoxal phosphate.

    GO - Molecular functioni

    1. glycine dehydrogenase (decarboxylating) activity Source: EcoCyc
    2. identical protein binding Source: IntAct
    3. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. glycine decarboxylation via glycine cleavage system Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciEcoCyc:GCVP-MONOMER.
    ECOL316407:JW2871-MONOMER.
    MetaCyc:GCVP-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycine dehydrogenase (decarboxylating) (EC:1.4.4.2)
    Alternative name(s):
    Glycine cleavage system P-protein
    Glycine decarboxylase
    Glycine dehydrogenase (aminomethyl-transferring)
    Gene namesi
    Name:gcvP
    Ordered Locus Names:b2903, JW2871
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11810. gcvP.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 957956Glycine dehydrogenase (decarboxylating)PRO_0000166913Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei708 – 7081N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PaxDbiP33195.
    PRIDEiP33195.

    Expressioni

    Inductioni

    By glycine.

    Gene expression databases

    GenevestigatoriP33195.

    Interactioni

    Subunit structurei

    The glycine cleavage system is composed of four proteins: P, T, L and H.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-551489,EBI-551489

    Protein-protein interaction databases

    DIPiDIP-9753N.
    IntActiP33195. 14 interactions.
    MINTiMINT-1318484.
    STRINGi511145.b2903.

    Structurei

    3D structure databases

    ProteinModelPortaliP33195.
    SMRiP33195. Positions 1-950.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GcvP family.Curated

    Phylogenomic databases

    eggNOGiCOG1003.
    HOGENOMiHOG000239369.
    KOiK00281.
    OMAiFDSRPYS.
    OrthoDBiEOG6HMXDX.
    PhylomeDBiP33195.

    Family and domain databases

    Gene3Di3.40.640.10. 2 hits.
    HAMAPiMF_00711. GcvP.
    InterProiIPR020580. GDC-P_N.
    IPR020581. GDC_P.
    IPR003437. GDC_P_homo.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PANTHERiPTHR11773. PTHR11773. 1 hit.
    PfamiPF02347. GDC-P. 2 hits.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 3 hits.
    TIGRFAMsiTIGR00461. gcvP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33195-1 [UniParc]FASTAAdd to Basket

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    MTQTLSQLEN SGAFIERHIG PDAAQQQEML NAVGAQSLNA LTGQIVPKDI    50
    QLATPPQVGA PATEYAALAE LKAIASRNKR FTSYIGMGYT AVQLPPVILR 100
    NMLENPGWYT AYTPYQPEVS QGRLEALLNF QQVTLDLTGL DMASASLLDE 150
    ATAAAEAMAM AKRVSKLKNA NRFFVASDVH PQTLDVVRTR AETFGFEVIV 200
    DDAQKVLDHQ DVFGVLLQQV GTTGEIHDYT ALISELKSRK IVVSVAADIM 250
    ALVLLTAPGK QGADIVFGSA QRFGVPMGYG GPHAAFFAAK DEYKRSMPGR 300
    IIGVSKDAAG NTALRMAMQT REQHIRREKA NSNICTSQVL LANIASLYAV 350
    YHGPVGLKRI ANRIHRLTDI LAAGLQQKGL KLRHAHYFDT LCVEVADKAG 400
    VLTRAEAAEI NLRSDILNAV GITLDETTTR ENVMQLFNVL LGDNHGLDID 450
    TLDKDVAHDS RSIQPAMLRD DEILTHPVFN RYHSETEMMR YMHSLERKDL 500
    ALNQAMIPLG SCTMKLNAAA EMIPITWPEF AELHPFCPPE QAEGYQQMIA 550
    QLADWLVKLT GYDAVCMQPN SGAQGEYAGL LAIRHYHESR NEGHRDICLI 600
    PASAHGTNPA SAHMAGMQVV VVACDKNGNI DLTDLRAKAE QAGDNLSCIM 650
    VTYPSTHGVY EETIREVCEV VHQFGGQVYL DGANMNAQVG ITSPGFIGAD 700
    VSHLNLHKTF CIPHGGGGPG MGPIGVKAHL APFVPGHSVV QIEGMLTRQG 750
    AVSAAPFGSA SILPISWMYI RMMGAEGLKK ASQVAILNAN YIASRLQDAF 800
    PVLYTGRDGR VAHECILDIR PLKEETGISE LDIAKRLIDY GFHAPTMSFP 850
    VAGTLMVEPT ESESKVELDR FIDAMLAIRA EIDQVKAGVW PLEDNPLVNA 900
    PHIQSELVAE WAHPYSREVA VFPAGVADKY WPTVKRLDDV YGDRNLFCSC 950
    VPISEYQ 957
    Length:957
    Mass (Da):104,376
    Last modified:January 23, 2007 - v3
    Checksum:i9983F4DCDC1095A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20872 Genomic DNA. Translation: AAA23867.1.
    X73958 Genomic DNA. Translation: CAA52146.1.
    U28377 Genomic DNA. Translation: AAA69071.1.
    U00096 Genomic DNA. Translation: AAC75941.1.
    AP009048 Genomic DNA. Translation: BAE76968.1.
    PIRiS36834.
    RefSeqiNP_417379.1. NC_000913.3.
    YP_491104.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75941; AAC75941; b2903.
    BAE76968; BAE76968; BAE76968.
    GeneIDi12930714.
    947394.
    KEGGiecj:Y75_p2835.
    eco:b2903.
    PATRICi32121220. VBIEscCol129921_2998.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20872 Genomic DNA. Translation: AAA23867.1 .
    X73958 Genomic DNA. Translation: CAA52146.1 .
    U28377 Genomic DNA. Translation: AAA69071.1 .
    U00096 Genomic DNA. Translation: AAC75941.1 .
    AP009048 Genomic DNA. Translation: BAE76968.1 .
    PIRi S36834.
    RefSeqi NP_417379.1. NC_000913.3.
    YP_491104.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P33195.
    SMRi P33195. Positions 1-950.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9753N.
    IntActi P33195. 14 interactions.
    MINTi MINT-1318484.
    STRINGi 511145.b2903.

    Proteomic databases

    PaxDbi P33195.
    PRIDEi P33195.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75941 ; AAC75941 ; b2903 .
    BAE76968 ; BAE76968 ; BAE76968 .
    GeneIDi 12930714.
    947394.
    KEGGi ecj:Y75_p2835.
    eco:b2903.
    PATRICi 32121220. VBIEscCol129921_2998.

    Organism-specific databases

    EchoBASEi EB1758.
    EcoGenei EG11810. gcvP.

    Phylogenomic databases

    eggNOGi COG1003.
    HOGENOMi HOG000239369.
    KOi K00281.
    OMAi FDSRPYS.
    OrthoDBi EOG6HMXDX.
    PhylomeDBi P33195.

    Enzyme and pathway databases

    BioCyci EcoCyc:GCVP-MONOMER.
    ECOL316407:JW2871-MONOMER.
    MetaCyc:GCVP-MONOMER.

    Miscellaneous databases

    PROi P33195.

    Gene expression databases

    Genevestigatori P33195.

    Family and domain databases

    Gene3Di 3.40.640.10. 2 hits.
    HAMAPi MF_00711. GcvP.
    InterProi IPR020580. GDC-P_N.
    IPR020581. GDC_P.
    IPR003437. GDC_P_homo.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view ]
    PANTHERi PTHR11773. PTHR11773. 1 hit.
    Pfami PF02347. GDC-P. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 3 hits.
    TIGRFAMsi TIGR00461. gcvP. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the Escherichia coli gcv operon."
      Stauffer L.T., Fogarty S.J., Stauffer G.V.
      Gene 142:17-22(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: K12.
    2. "Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system."
      Okamura-Ikeda K., Ohmura Y., Fujiwara K., Motokawa Y.
      Eur. J. Biochem. 216:539-548(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiGCSP_ECOLI
    AccessioniPrimary (citable) accession number: P33195
    Secondary accession number(s): Q2M9T8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 117 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3