Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycine dehydrogenase (decarboxylating)

Gene

gcvP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.UniRule annotation1 Publication

Catalytic activityi

Glycine + [glycine-cleavage complex H protein]-N6-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N6-dihydrolipoyl-L-lysine + CO2.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

GO - Molecular functioni

  • glycine dehydrogenase (decarboxylating) activity Source: EcoCyc
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:GCVP-MONOMER
MetaCyc:GCVP-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine dehydrogenase (decarboxylating)UniRule annotation (EC:1.4.4.2UniRule annotation)
Alternative name(s):
Glycine cleavage system P-proteinUniRule annotation
Glycine decarboxylaseUniRule annotation
Glycine dehydrogenase (aminomethyl-transferring)UniRule annotation
Gene namesi
Name:gcvP1 Publication
Ordered Locus Names:b2903, JW2871
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11810 gcvP

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001669132 – 957Glycine dehydrogenase (decarboxylating)Add BLAST956

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei708N6-(pyridoxal phosphate)lysineUniRule annotation1

Proteomic databases

EPDiP33195
PaxDbiP33195
PRIDEiP33195

Expressioni

Inductioni

By glycine.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-551489,EBI-551489

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4259708, 49 interactors
DIPiDIP-9753N
IntActiP33195, 14 interactors
STRINGi316385.ECDH10B_3077

Structurei

3D structure databases

ProteinModelPortaliP33195
SMRiP33195
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvP family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105CBI Bacteria
COG0403 LUCA
COG1003 LUCA
HOGENOMiHOG000239369
InParanoidiP33195
KOiK00281
OMAiQTMVCDL
PhylomeDBiP33195

Family and domain databases

CDDicd00613 GDC-P, 2 hits
Gene3Di3.40.640.10, 2 hits
3.90.1150.10, 1 hit
HAMAPiMF_00711 GcvP, 1 hit
InterProiView protein in InterPro
IPR003437 GcvP
IPR020581 GDC_P
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11773 PTHR11773, 1 hit
PfamiView protein in Pfam
PF02347 GDC-P, 2 hits
SUPFAMiSSF53383 SSF53383, 3 hits
TIGRFAMsiTIGR00461 gcvP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33195-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQTLSQLEN SGAFIERHIG PDAAQQQEML NAVGAQSLNA LTGQIVPKDI
60 70 80 90 100
QLATPPQVGA PATEYAALAE LKAIASRNKR FTSYIGMGYT AVQLPPVILR
110 120 130 140 150
NMLENPGWYT AYTPYQPEVS QGRLEALLNF QQVTLDLTGL DMASASLLDE
160 170 180 190 200
ATAAAEAMAM AKRVSKLKNA NRFFVASDVH PQTLDVVRTR AETFGFEVIV
210 220 230 240 250
DDAQKVLDHQ DVFGVLLQQV GTTGEIHDYT ALISELKSRK IVVSVAADIM
260 270 280 290 300
ALVLLTAPGK QGADIVFGSA QRFGVPMGYG GPHAAFFAAK DEYKRSMPGR
310 320 330 340 350
IIGVSKDAAG NTALRMAMQT REQHIRREKA NSNICTSQVL LANIASLYAV
360 370 380 390 400
YHGPVGLKRI ANRIHRLTDI LAAGLQQKGL KLRHAHYFDT LCVEVADKAG
410 420 430 440 450
VLTRAEAAEI NLRSDILNAV GITLDETTTR ENVMQLFNVL LGDNHGLDID
460 470 480 490 500
TLDKDVAHDS RSIQPAMLRD DEILTHPVFN RYHSETEMMR YMHSLERKDL
510 520 530 540 550
ALNQAMIPLG SCTMKLNAAA EMIPITWPEF AELHPFCPPE QAEGYQQMIA
560 570 580 590 600
QLADWLVKLT GYDAVCMQPN SGAQGEYAGL LAIRHYHESR NEGHRDICLI
610 620 630 640 650
PASAHGTNPA SAHMAGMQVV VVACDKNGNI DLTDLRAKAE QAGDNLSCIM
660 670 680 690 700
VTYPSTHGVY EETIREVCEV VHQFGGQVYL DGANMNAQVG ITSPGFIGAD
710 720 730 740 750
VSHLNLHKTF CIPHGGGGPG MGPIGVKAHL APFVPGHSVV QIEGMLTRQG
760 770 780 790 800
AVSAAPFGSA SILPISWMYI RMMGAEGLKK ASQVAILNAN YIASRLQDAF
810 820 830 840 850
PVLYTGRDGR VAHECILDIR PLKEETGISE LDIAKRLIDY GFHAPTMSFP
860 870 880 890 900
VAGTLMVEPT ESESKVELDR FIDAMLAIRA EIDQVKAGVW PLEDNPLVNA
910 920 930 940 950
PHIQSELVAE WAHPYSREVA VFPAGVADKY WPTVKRLDDV YGDRNLFCSC

VPISEYQ
Length:957
Mass (Da):104,376
Last modified:January 23, 2007 - v3
Checksum:i9983F4DCDC1095A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20872 Genomic DNA Translation: AAA23867.1
X73958 Genomic DNA Translation: CAA52146.1
U28377 Genomic DNA Translation: AAA69071.1
U00096 Genomic DNA Translation: AAC75941.1
AP009048 Genomic DNA Translation: BAE76968.1
PIRiS36834
RefSeqiNP_417379.1, NC_000913.3
WP_000195062.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75941; AAC75941; b2903
BAE76968; BAE76968; BAE76968
GeneIDi947394
KEGGiecj:JW2871
eco:b2903
PATRICifig|1411691.4.peg.3829

Entry informationi

Entry nameiGCSP_ECOLI
AccessioniPrimary (citable) accession number: P33195
Secondary accession number(s): Q2M9T8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health