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P33195

- GCSP_ECOLI

UniProt

P33195 - GCSP_ECOLI

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Protein

Glycine dehydrogenase (decarboxylating)

Gene

gcvP

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.

Catalytic activityi

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2.

Cofactori

GO - Molecular functioni

  1. glycine dehydrogenase (decarboxylating) activity Source: EcoCyc
  2. identical protein binding Source: IntAct
  3. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. glycine decarboxylation via glycine cleavage system Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:GCVP-MONOMER.
ECOL316407:JW2871-MONOMER.
MetaCyc:GCVP-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine dehydrogenase (decarboxylating) (EC:1.4.4.2)
Alternative name(s):
Glycine cleavage system P-protein
Glycine decarboxylase
Glycine dehydrogenase (aminomethyl-transferring)
Gene namesi
Name:gcvP
Ordered Locus Names:b2903, JW2871
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11810. gcvP.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 957956Glycine dehydrogenase (decarboxylating)PRO_0000166913Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei708 – 7081N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiP33195.
PRIDEiP33195.

Expressioni

Inductioni

By glycine.

Gene expression databases

GenevestigatoriP33195.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-551489,EBI-551489

Protein-protein interaction databases

DIPiDIP-9753N.
IntActiP33195. 14 interactions.
MINTiMINT-1318484.
STRINGi511145.b2903.

Structurei

3D structure databases

ProteinModelPortaliP33195.
SMRiP33195. Positions 1-950.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvP family.Curated

Phylogenomic databases

eggNOGiCOG1003.
HOGENOMiHOG000239369.
InParanoidiP33195.
KOiK00281.
OMAiFDSRPYS.
OrthoDBiEOG6HMXDX.
PhylomeDBiP33195.

Family and domain databases

Gene3Di3.40.640.10. 2 hits.
HAMAPiMF_00711. GcvP.
InterProiIPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11773. PTHR11773. 1 hit.
PfamiPF02347. GDC-P. 2 hits.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 3 hits.
TIGRFAMsiTIGR00461. gcvP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33195-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQTLSQLEN SGAFIERHIG PDAAQQQEML NAVGAQSLNA LTGQIVPKDI
60 70 80 90 100
QLATPPQVGA PATEYAALAE LKAIASRNKR FTSYIGMGYT AVQLPPVILR
110 120 130 140 150
NMLENPGWYT AYTPYQPEVS QGRLEALLNF QQVTLDLTGL DMASASLLDE
160 170 180 190 200
ATAAAEAMAM AKRVSKLKNA NRFFVASDVH PQTLDVVRTR AETFGFEVIV
210 220 230 240 250
DDAQKVLDHQ DVFGVLLQQV GTTGEIHDYT ALISELKSRK IVVSVAADIM
260 270 280 290 300
ALVLLTAPGK QGADIVFGSA QRFGVPMGYG GPHAAFFAAK DEYKRSMPGR
310 320 330 340 350
IIGVSKDAAG NTALRMAMQT REQHIRREKA NSNICTSQVL LANIASLYAV
360 370 380 390 400
YHGPVGLKRI ANRIHRLTDI LAAGLQQKGL KLRHAHYFDT LCVEVADKAG
410 420 430 440 450
VLTRAEAAEI NLRSDILNAV GITLDETTTR ENVMQLFNVL LGDNHGLDID
460 470 480 490 500
TLDKDVAHDS RSIQPAMLRD DEILTHPVFN RYHSETEMMR YMHSLERKDL
510 520 530 540 550
ALNQAMIPLG SCTMKLNAAA EMIPITWPEF AELHPFCPPE QAEGYQQMIA
560 570 580 590 600
QLADWLVKLT GYDAVCMQPN SGAQGEYAGL LAIRHYHESR NEGHRDICLI
610 620 630 640 650
PASAHGTNPA SAHMAGMQVV VVACDKNGNI DLTDLRAKAE QAGDNLSCIM
660 670 680 690 700
VTYPSTHGVY EETIREVCEV VHQFGGQVYL DGANMNAQVG ITSPGFIGAD
710 720 730 740 750
VSHLNLHKTF CIPHGGGGPG MGPIGVKAHL APFVPGHSVV QIEGMLTRQG
760 770 780 790 800
AVSAAPFGSA SILPISWMYI RMMGAEGLKK ASQVAILNAN YIASRLQDAF
810 820 830 840 850
PVLYTGRDGR VAHECILDIR PLKEETGISE LDIAKRLIDY GFHAPTMSFP
860 870 880 890 900
VAGTLMVEPT ESESKVELDR FIDAMLAIRA EIDQVKAGVW PLEDNPLVNA
910 920 930 940 950
PHIQSELVAE WAHPYSREVA VFPAGVADKY WPTVKRLDDV YGDRNLFCSC

VPISEYQ
Length:957
Mass (Da):104,376
Last modified:January 23, 2007 - v3
Checksum:i9983F4DCDC1095A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20872 Genomic DNA. Translation: AAA23867.1.
X73958 Genomic DNA. Translation: CAA52146.1.
U28377 Genomic DNA. Translation: AAA69071.1.
U00096 Genomic DNA. Translation: AAC75941.1.
AP009048 Genomic DNA. Translation: BAE76968.1.
PIRiS36834.
RefSeqiNP_417379.1. NC_000913.3.
YP_491104.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75941; AAC75941; b2903.
BAE76968; BAE76968; BAE76968.
GeneIDi12930714.
947394.
KEGGiecj:Y75_p2835.
eco:b2903.
PATRICi32121220. VBIEscCol129921_2998.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20872 Genomic DNA. Translation: AAA23867.1 .
X73958 Genomic DNA. Translation: CAA52146.1 .
U28377 Genomic DNA. Translation: AAA69071.1 .
U00096 Genomic DNA. Translation: AAC75941.1 .
AP009048 Genomic DNA. Translation: BAE76968.1 .
PIRi S36834.
RefSeqi NP_417379.1. NC_000913.3.
YP_491104.1. NC_007779.1.

3D structure databases

ProteinModelPortali P33195.
SMRi P33195. Positions 1-950.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9753N.
IntActi P33195. 14 interactions.
MINTi MINT-1318484.
STRINGi 511145.b2903.

Proteomic databases

PaxDbi P33195.
PRIDEi P33195.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75941 ; AAC75941 ; b2903 .
BAE76968 ; BAE76968 ; BAE76968 .
GeneIDi 12930714.
947394.
KEGGi ecj:Y75_p2835.
eco:b2903.
PATRICi 32121220. VBIEscCol129921_2998.

Organism-specific databases

EchoBASEi EB1758.
EcoGenei EG11810. gcvP.

Phylogenomic databases

eggNOGi COG1003.
HOGENOMi HOG000239369.
InParanoidi P33195.
KOi K00281.
OMAi FDSRPYS.
OrthoDBi EOG6HMXDX.
PhylomeDBi P33195.

Enzyme and pathway databases

BioCyci EcoCyc:GCVP-MONOMER.
ECOL316407:JW2871-MONOMER.
MetaCyc:GCVP-MONOMER.

Miscellaneous databases

PROi P33195.

Gene expression databases

Genevestigatori P33195.

Family and domain databases

Gene3Di 3.40.640.10. 2 hits.
HAMAPi MF_00711. GcvP.
InterProi IPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR003437. GDC_P_homo.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view ]
PANTHERi PTHR11773. PTHR11773. 1 hit.
Pfami PF02347. GDC-P. 2 hits.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 3 hits.
TIGRFAMsi TIGR00461. gcvP. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the Escherichia coli gcv operon."
    Stauffer L.T., Fogarty S.J., Stauffer G.V.
    Gene 142:17-22(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: K12.
  2. "Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system."
    Okamura-Ikeda K., Ohmura Y., Fujiwara K., Motokawa Y.
    Eur. J. Biochem. 216:539-548(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiGCSP_ECOLI
AccessioniPrimary (citable) accession number: P33195
Secondary accession number(s): Q2M9T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3