Reviewed, UniProtKB/Swiss-Prot P33186 (RIPG_GELMU)

Names and origin

Protein names

Recommended name:
    Ribosome-inactivating protein gelonin
    EC=3.2.2.22
Alternative name(s):
    rRNA N-glycosidase

Gene names

Name:

GEL

Organism

Gelonium multiflorum (Euphorbiaceae himalaya)

Taxonomic identifier

3979 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Euphorbiaceae › Crotonoideae › Gelonieae › Gelonium

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Propeptide	27 – 46	20	Ref.2		PRO_0000030786
	Chain	47 – 297	251	Ribosome-inactivating protein gelonin		PRO_0000030787
	Propeptide	298 – 316	19	Removed in mature form		PRO_0000030788
Sites
	Active site	212	1
Amino acid modifications
	Glycosylation	235	1	N-linked (GlcNAc...)
	Disulfide bond	90 ↔ 96
Experimental info
	Sequence conflict	90	1	C → K AA sequence Ref.2
	Sequence conflict	93	1	P → D AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P33186-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 1252F3E710901B85	FASTA	316	35,419
	10 20 30 40 50 60 MKGNMKVYWI KIAVATWFCC TTIVLGSTAR IFSLPTNDEE ETSKTLGLDT VSFSTKGATY 70 80 90 100 110 120 ITYVNFLNEL RVKLKPEGNS HGIPLLRKKC DDPGKCFVLV ALSNDNGQLA EIAIDVTSVY 130 140 150 160 170 180 VVGYQVRNRS YFFKDAPDAA YEGLFKNTIK TRLHFGGSYP SLEGEKAYRE TTDLGIEPLR 190 200 210 220 230 240 IGIKKLDENA IDNYKPTEIA SSLLVVIQMV SEAARFTFIE NQIRNNFQQR IRPANNTISL 250 260 270 280 290 300 ENKWGKLSFQ IRTSGANGMF SEAVELERAN GKKYYVTAVD QVKPKIALLK FVDKDPKTSL 310 AAELIIQNYE SLVGFD

References

[1]	"Cloning and expression of a gene encoding gelonin, a ribosome-inactivating protein from Gelonium multiflorum." Nolan P.A., Garrison D.A., Better M. Gene 134:223-227(1993) [PubMed: 7916721] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"N-terminal sequence of some ribosome-inactivating proteins." Montecucchi P.-C., Lazzarini A.M., Barbieri L., Stirpe F., Soria M., Lappi D. Int. J. Pept. Protein Res. 33:263-267(1989) [PubMed: 2753596] [Abstract] Cited for: PROTEIN SEQUENCE OF 47-93. Tissue: Seed.
[3]	"X-ray structure of gelonin at 1.8-A resolution." Hosur M.V., Nair B., Satyamurthy P., Misquith S., Surolia A., Kannan K.K. J. Mol. Biol. 250:368-380(1995) [PubMed: 7608981] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Cross-references

Sequence databases
EMBLGenBankDDBJ	L12243 mRNA. Translation: AAA16312.1.
PIR	JT0753.
3D structure databases
HSSP	HSSP built from PDB template 1MRJ based on UniProtKB P09989.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 273556.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIPG_GELMU

Accession

Primary (citable) accession number: P33186

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)