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Protein

Kinesin heavy chain isoform 5A

Gene

Kif5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 938ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, plus-end-directed Source: GO_Central
  • kinesin binding Source: UniProtKB
  • microtubule binding Source: MGI
  • microtubule motor activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-5625970. RHO GTPases activate KTN1.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-MMU-983189. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin heavy chain isoform 5A
Alternative name(s):
Kinesin heavy chain neuron-specific 1
Neuronal kinesin heavy chain
Short name:
NKHC
Gene namesi
Name:Kif5a
Synonyms:Kiaa4086, Kif5, Nkhc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:109564. Kif5a.

Subcellular locationi

GO - Cellular componenti

  • ciliary rootlet Source: MGI
  • cytoplasm Source: MGI
  • kinesin complex Source: MGI
  • membrane Source: MGI
  • microtubule Source: UniProtKB-KW
  • neuronal cell body Source: MGI
  • neuron projection Source: MGI
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Death shortly after birth. Neuron-specific deletion within the first 3 weeks after birth is lethal in 75% of animals. Surviving animals show accumulation of neurofilament proteins in neuronal soma, age-dependent sensory neuron degeneration, loss of large caliber axons, and hind limb paralysis with a stronger effect on sensory neurons compared with motor neurons.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 10271026Kinesin heavy chain isoform 5APRO_0000125354Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei397 – 3971PhosphothreonineCombined sources
Modified residuei931 – 9311PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP33175.
PaxDbiP33175.
PeptideAtlasiP33175.
PRIDEiP33175.

PTM databases

iPTMnetiP33175.
PhosphoSiteiP33175.

Expressioni

Gene expression databases

BgeeiENSMUSG00000074657.
CleanExiMM_KIF5A.
GenevisibleiP33175. MM.

Interactioni

Subunit structurei

Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 (PubMed:11986669). Interacts with FMR1 (via C-terminus); this interaction is increased in a mGluR-dependent manner (PubMed:18539120).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ANKRD27Q96NW42EBI-349710,EBI-6125599From a different organism.
Dctn1O087882EBI-349710,EBI-776180
DtnbO705854EBI-349710,EBI-349714

GO - Molecular functioni

  • kinesin binding Source: UniProtKB
  • microtubule binding Source: MGI

Protein-protein interaction databases

BioGridi200945. 7 interactions.
IntActiP33175. 7 interactions.
MINTiMINT-236733.
STRINGi10090.ENSMUSP00000096775.

Structurei

3D structure databases

ProteinModelPortaliP33175.
SMRiP33175. Positions 7-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 327319Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni174 – 315142Microtubule-bindingAdd
BLAST
Regioni907 – 1027121GlobularAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili331 – 906576Add
BLAST

Domaini

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129683.
HOGENOMiHOG000216718.
HOVERGENiHBG006210.
InParanoidiP33175.
KOiK10396.
OMAiMENHREA.
OrthoDBiEOG091G02A2.
PhylomeDBiP33175.
TreeFamiTF105225.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETNNECSI KVLCRFRPLN QAEILRGDKF IPIFQGDDSV IIGGKPYVFD
60 70 80 90 100
RVFPPNTTQE QVYHACAMQI VKDVLAGYNG TIFAYGQTSS GKTHTMEGKL
110 120 130 140 150
HDPQLMGIIP RIARDIFNHI YSMDENLEFH IKVSYFEIYL DKIRDLLDVT
160 170 180 190 200
KTNLSVHEDK NRVPFVKGCT ERFVSSPEEI LDVIDEGKSN RHVAVTNMNE
210 220 230 240 250
HSSRSHSIFL INIKQENVET EQKLSGKLYL VDLAGSEKVS KTGAEGAVLD
260 270 280 290 300
EAKNINKSLS ALGNVISALA EGTKSYVPYR DSKMTRILQD SLGGNCRTTM
310 320 330 340 350
FICCSPSSYN DAETKSTLMF GQRAKTIKNT ASVNLELTAE QWKKKYEKEK
360 370 380 390 400
EKTKAQKETI AKLEAELSRW RNGENVPETE RLAGEDSALG AELCEETPVN
410 420 430 440 450
DNSSIVVRIA PEERQKYEEE IRRLYKQLDD KDDEINQQSQ LIEKLKQQML
460 470 480 490 500
DQEELLVSTR GDNEKVQREL SHLQSENDAA KDEVKEVLQA LEELAVNYDQ
510 520 530 540 550
KSQEVEEKSQ QNQLLVDELS QKVATMLSLE SELQRLQEVS GHQRKRIAEV
560 570 580 590 600
LNGLMRDLSE FSVIVGNGEI KLPVEISGAI EEEFTVARLY ISKIKSEVKS
610 620 630 640 650
VVKRCRQLEN LQVECHRKME VTGRELSSCQ LLISQHEAKI RSLTEYMQTV
660 670 680 690 700
ELKKRHLEES YDSLSDELAR LQAHETVHEV ALKDKEPDTQ DAEEVKKALE
710 720 730 740 750
LQMENHREAH HRQLARLRDE INEKQKTIDE LKDLNQKLQL ELEKLQADYE
760 770 780 790 800
RLKNEENEKS AKLQELTFLY ERHEQSKQDL KGLEETVARE LQTLHNLRKL
810 820 830 840 850
FVQDVTTRVK KSAEMEPEDS GGIHSQKQKI SFLENNLEQL TKVHKQLVRD
860 870 880 890 900
NADLRCELPK LEKRLRATAE RVKALEGALK EAKEGAMKDK RRYQQEVDRI
910 920 930 940 950
KEAVRYKSSG KRGHSAQIAK PVRPGHYPAS SPTNPYGTRS PECISYTNNL
960 970 980 990 1000
FQNYQNLHLQ AAPSSTSDMY FASSGATSVA PLASYQKANM DNGNATDIND
1010 1020
NRSDLPCGYE AEDQAKLFPL HQETAAS
Length:1,027
Mass (Da):117,019
Last modified:June 27, 2006 - v3
Checksum:i419AD8C2D747DA0A
GO

Sequence cautioni

The sequence BAD90503 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti146 – 1461L → P (PubMed:1447303).Curated
Sequence conflicti282 – 2821S → T in AAC79803 (PubMed:9782088).Curated
Sequence conflicti362 – 3632KL → NV in AAC79803 (PubMed:9782088).Curated
Sequence conflicti727 – 7271T → I in BAD90503 (Ref. 3) Curated
Sequence conflicti976 – 9761A → R in AAC79803 (PubMed:9782088).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067179 mRNA. Translation: AAC79803.1.
AK147352 mRNA. Translation: BAE27862.1.
AK147660 mRNA. Translation: BAE28054.1.
AK147707 mRNA. Translation: BAE28087.1.
AK220479 mRNA. Translation: BAD90503.1. Different initiation.
BC058396 mRNA. Translation: AAH58396.1.
CCDSiCCDS24233.1.
PIRiC44259.
RefSeqiNP_001034089.1. NM_001039000.4.
NP_032473.2. NM_008447.4.
UniGeneiMm.30355.

Genome annotation databases

EnsembliENSMUST00000099172; ENSMUSP00000096775; ENSMUSG00000074657.
GeneIDi16572.
KEGGimmu:16572.
UCSCiuc007his.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067179 mRNA. Translation: AAC79803.1.
AK147352 mRNA. Translation: BAE27862.1.
AK147660 mRNA. Translation: BAE28054.1.
AK147707 mRNA. Translation: BAE28087.1.
AK220479 mRNA. Translation: BAD90503.1. Different initiation.
BC058396 mRNA. Translation: AAH58396.1.
CCDSiCCDS24233.1.
PIRiC44259.
RefSeqiNP_001034089.1. NM_001039000.4.
NP_032473.2. NM_008447.4.
UniGeneiMm.30355.

3D structure databases

ProteinModelPortaliP33175.
SMRiP33175. Positions 7-372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200945. 7 interactions.
IntActiP33175. 7 interactions.
MINTiMINT-236733.
STRINGi10090.ENSMUSP00000096775.

PTM databases

iPTMnetiP33175.
PhosphoSiteiP33175.

Proteomic databases

MaxQBiP33175.
PaxDbiP33175.
PeptideAtlasiP33175.
PRIDEiP33175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099172; ENSMUSP00000096775; ENSMUSG00000074657.
GeneIDi16572.
KEGGimmu:16572.
UCSCiuc007his.2. mouse.

Organism-specific databases

CTDi3798.
MGIiMGI:109564. Kif5a.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129683.
HOGENOMiHOG000216718.
HOVERGENiHBG006210.
InParanoidiP33175.
KOiK10396.
OMAiMENHREA.
OrthoDBiEOG091G02A2.
PhylomeDBiP33175.
TreeFamiTF105225.

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-5625970. RHO GTPases activate KTN1.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-MMU-983189. Kinesins.

Miscellaneous databases

ChiTaRSiKif5a. mouse.
PROiP33175.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074657.
CleanExiMM_KIF5A.
GenevisibleiP33175. MM.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIF5A_MOUSE
AccessioniPrimary (citable) accession number: P33175
Secondary accession number(s): Q5DTP1, Q6PDY7, Q9Z2F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 27, 2006
Last modified: September 7, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.