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Protein

Chromosome-associated kinesin KIF4

Gene

Kif4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for mitotic chromosomal positioning and bipolar spindle stabilization.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi88 – 958ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-437239. Recycling pathway of L1.
R-MMU-983189. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome-associated kinesin KIF4
Alternative name(s):
Chromokinesin
Gene namesi
Name:Kif4
Synonyms:Kif4a, Kns4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:108389. Kif4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12311231Chromosome-associated kinesin KIF4PRO_0000125438Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei395 – 3951PhosphoserineCombined sources
Modified residuei800 – 8001PhosphothreonineCombined sources
Modified residuei802 – 8021PhosphoserineCombined sources
Modified residuei811 – 8111PhosphoserineCombined sources
Modified residuei816 – 8161PhosphoserineCombined sources
Modified residuei1224 – 12241PhosphoserineCombined sources
Modified residuei1230 – 12301PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP33174.
MaxQBiP33174.
PaxDbiP33174.
PRIDEiP33174.

PTM databases

iPTMnetiP33174.
PhosphoSiteiP33174.

Expressioni

Tissue specificityi

Expressed in pyramidal cells in juvenile hippocampus, granular cells in juvenile cerebellar cortex and in adult spleen.1 Publication

Gene expression databases

BgeeiP33174.
CleanExiMM_KIF4.
GenevisibleiP33174. MM.

Interactioni

Protein-protein interaction databases

BioGridi200944. 31 interactions.
IntActiP33174. 28 interactions.
STRINGi10090.ENSMUSP00000048383.

Structurei

Secondary structure

1
1231
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93Combined sources
Beta strandi11 – 166Combined sources
Helixi21 – 255Combined sources
Beta strandi32 – 343Combined sources
Beta strandi38 – 436Combined sources
Turni44 – 463Combined sources
Beta strandi47 – 504Combined sources
Beta strandi52 – 554Combined sources
Helixi61 – 688Combined sources
Helixi70 – 767Combined sources
Turni77 – 793Combined sources
Beta strandi82 – 876Combined sources
Helixi94 – 985Combined sources
Turni99 – 1013Combined sources
Helixi104 – 1063Combined sources
Helixi114 – 12815Combined sources
Beta strandi132 – 14413Combined sources
Beta strandi147 – 1504Combined sources
Beta strandi163 – 1664Combined sources
Turni168 – 1703Combined sources
Beta strandi172 – 1754Combined sources
Beta strandi180 – 1823Combined sources
Helixi185 – 19814Combined sources
Beta strandi201 – 2044Combined sources
Beta strandi207 – 2115Combined sources
Beta strandi213 – 22513Combined sources
Beta strandi228 – 24114Combined sources
Helixi248 – 2514Combined sources
Helixi255 – 27925Combined sources
Helixi289 – 2913Combined sources
Helixi293 – 2975Combined sources
Turni298 – 3036Combined sources
Beta strandi304 – 31411Combined sources
Helixi318 – 3203Combined sources
Helixi321 – 33414Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZFCX-ray1.80A1-344[»]
3ZFDX-ray1.71A1-344[»]
ProteinModelPortaliP33174.
SMRiP33174. Positions 4-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 337329Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1001 – 1231231GlobularAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili351 – 1000650Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Chromokinesin subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0244. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129683.
HOGENOMiHOG000048108.
HOVERGENiHBG052256.
InParanoidiP33174.
KOiK10395.
OMAiHADEEWI.
OrthoDBiEOG7P8P9J.
TreeFamiTF105224.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
IPR033467. Tesmin/TSO1-like_CXC.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM01114. CXC. 1 hit.
SM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEEVKGIPV RVALRCRPLV SKEIKEGCQT CLSFVPGEPQ VVVGNDKSFT
60 70 80 90 100
YDFVFDPSTE QEEVFNTAVA PLIKGVFKGY NATVLAYGQT GSGKTYSMGG
110 120 130 140 150
AYTAEQEHDS AIGVIPRVIQ LLFKEINKKS DFEFTLKVSY LEIYNEEILD
160 170 180 190 200
LLCSSREKAT QINIREDPKE GIKIVGLTEK TVLVASDTVS CLEQGNNSRT
210 220 230 240 250
VASTAMNSQS SRSHAIFTIS IEQRKKNDKN SSFRSKLHLV DLAGSERQKK
260 270 280 290 300
TKAEGDRLRE GININRGLLC LGNVISALGD DKKGNFVPYR DSKLTRLLQD
310 320 330 340 350
SLGGNSHTLM IACVSPADSN LEETLNTLRY ADRARKIKNK PIINIDPQAA
360 370 380 390 400
ELNHLKQQVQ QLQILLLQAH GGTLPGDINV EPSENLQSLM EKNQSLVEEN
410 420 430 440 450
EKLSRGLSEA AGQTAQMLER IILTEQANEK MNAKLEELRR HAACKVDLQK
460 470 480 490 500
LVETLEDQEL KENIEIICNL QQVIAQLSDE AAACMTATID TAGEADTQVQ
510 520 530 540 550
SSPDTSRSSD VFSTQHALRQ AQMSKELIEL NKALALKEAL AKKMTQNDNQ
560 570 580 590 600
LQPIQFQYQD NIKNLESEVL SLQREKEELV LELQTAKKDA NQAKLSERRR
610 620 630 640 650
KRLQELEGQI ADLKKKLQEQ SKLLKLKEST EHTVSKLNQE IRMMKNQRVQ
660 670 680 690 700
LMRQMKEDAE KFRQWKQQKD KEVIQLKERD RKRQYELLKL ERNFQKQSNV
710 720 730 740 750
LRRKTEEAAA ANKRLKDALQ KQKEVAEKRK ETQSRGMEST AARMKNWLGN
760 770 780 790 800
EIEVMVSTEE AKRHLNGLLE ERKILAQDVA QLKEKRESGE NPPLKLRRRT
810 820 830 840 850
FSYDEIHGQD SGAEDSIAKQ IESLETELEL RSAQIADLQQ KLLDAESEDR
860 870 880 890 900
PKQRWESIAT ILEAKCAIKY LVGELVSSKI LVSKLESSLN QSKASCIDVQ
910 920 930 940 950
KMLFEEQNHF AKIETELKEE LVKVEQQHQE KVLYLLSQLQ QSQMTEKQLE
960 970 980 990 1000
ESVSEKEQQL LSTLKCQEEE LRKMQEVCEQ NQQLLQENSA IKQKLTLLQV
1010 1020 1030 1040 1050
ASKQKPHLTR NIFQSPDSSF EYIPPKPKPC RIKEKCLEQS FAVEGLQYYS
1060 1070 1080 1090 1100
EPSVAEQDNE DSDDHADEEW IPTKLVKVSK KSIQGCSCKG WCGNKQCGCR
1110 1120 1130 1140 1150
KQKSDCNVSC SCDPTKCRNR HQNQDNSDAI ELNQDSENSF KLEDPTEVTS
1160 1170 1180 1190 1200
GLSFFHPICA TPSSKILKEM CDADQVQLKQ PVFVSSSDHP ELKSIASESQ
1210 1220 1230
ENKAIGKKKK RALASNTSFF SGCSPIQEES H
Length:1,231
Mass (Da):139,519
Last modified:July 27, 2011 - v3
Checksum:iAA3C5E2E52678DE7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti112 – 1121I → S (PubMed:19468303).Curated
Sequence conflicti1044 – 10452EG → GE in BAA02167 (PubMed:7929562).Curated
Sequence conflicti1182 – 11821V → M in BAA02167 (PubMed:7929562).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12646 mRNA. Translation: BAA02167.1.
BX005480, BX276129 Genomic DNA. Translation: CAM15067.1.
BX276129, BX005480 Genomic DNA. Translation: CAM17678.1.
CH466564 Genomic DNA. Translation: EDL14171.1.
BC050946 mRNA. Translation: AAH50946.1.
CCDSiCCDS30305.1.
PIRiA54803.
RefSeqiNP_032472.2. NM_008446.2.
UniGeneiMm.383091.

Genome annotation databases

EnsembliENSMUST00000048962; ENSMUSP00000048383; ENSMUSG00000034311.
GeneIDi16571.
KEGGimmu:16571.
UCSCiuc009twh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12646 mRNA. Translation: BAA02167.1.
BX005480, BX276129 Genomic DNA. Translation: CAM15067.1.
BX276129, BX005480 Genomic DNA. Translation: CAM17678.1.
CH466564 Genomic DNA. Translation: EDL14171.1.
BC050946 mRNA. Translation: AAH50946.1.
CCDSiCCDS30305.1.
PIRiA54803.
RefSeqiNP_032472.2. NM_008446.2.
UniGeneiMm.383091.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZFCX-ray1.80A1-344[»]
3ZFDX-ray1.71A1-344[»]
ProteinModelPortaliP33174.
SMRiP33174. Positions 4-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200944. 31 interactions.
IntActiP33174. 28 interactions.
STRINGi10090.ENSMUSP00000048383.

PTM databases

iPTMnetiP33174.
PhosphoSiteiP33174.

Proteomic databases

EPDiP33174.
MaxQBiP33174.
PaxDbiP33174.
PRIDEiP33174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048962; ENSMUSP00000048383; ENSMUSG00000034311.
GeneIDi16571.
KEGGimmu:16571.
UCSCiuc009twh.2. mouse.

Organism-specific databases

CTDi16571.
MGIiMGI:108389. Kif4.

Phylogenomic databases

eggNOGiKOG0244. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129683.
HOGENOMiHOG000048108.
HOVERGENiHBG052256.
InParanoidiP33174.
KOiK10395.
OMAiHADEEWI.
OrthoDBiEOG7P8P9J.
TreeFamiTF105224.

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-437239. Recycling pathway of L1.
R-MMU-983189. Kinesins.

Miscellaneous databases

NextBioi290085.
PROiP33174.
SOURCEiSearch...

Gene expression databases

BgeeiP33174.
CleanExiMM_KIF4.
GenevisibleiP33174. MM.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
IPR033467. Tesmin/TSO1-like_CXC.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM01114. CXC. 1 hit.
SM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel microtubule-based motor protein (KIF4) for organelle transports, whose expression is regulated developmentally."
    Sekine Y., Okada Y., Noda Y., Kondo S., Aizawa H., Takemura R., Hirokawa N.
    J. Cell Biol. 127:187-201(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: ICR.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  5. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 91-240.
    Tissue: Brain.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-802, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-395; THR-800; SER-811; SER-816; SER-1224 AND SER-1230, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung and Spleen.

Entry informationi

Entry nameiKIF4_MOUSE
AccessioniPrimary (citable) accession number: P33174
Secondary accession number(s): Q80YP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.