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Protein

Chromosome-associated kinesin KIF4

Gene

Kif4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for mitotic chromosomal positioning and bipolar spindle stabilization.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 95ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-437239. Recycling pathway of L1.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-MMU-983189. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome-associated kinesin KIF4
Alternative name(s):
Chromokinesin
Gene namesi
Name:Kif4
Synonyms:Kif4a, Kns4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:108389. Kif4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001254381 – 1231Chromosome-associated kinesin KIF4Add BLAST1231

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei395PhosphoserineCombined sources1
Modified residuei800PhosphothreonineCombined sources1
Modified residuei802PhosphoserineCombined sources1
Modified residuei811PhosphoserineCombined sources1
Modified residuei816PhosphoserineCombined sources1
Modified residuei1224PhosphoserineCombined sources1
Modified residuei1230PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP33174.
MaxQBiP33174.
PaxDbiP33174.
PeptideAtlasiP33174.
PRIDEiP33174.

PTM databases

iPTMnetiP33174.
PhosphoSitePlusiP33174.

Expressioni

Tissue specificityi

Expressed in pyramidal cells in juvenile hippocampus, granular cells in juvenile cerebellar cortex and in adult spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000034311.
CleanExiMM_KIF4.
GenevisibleiP33174. MM.

Interactioni

Protein-protein interaction databases

BioGridi200944. 31 interactors.
IntActiP33174. 28 interactors.
STRINGi10090.ENSMUSP00000048383.

Structurei

Secondary structure

11231
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Beta strandi11 – 16Combined sources6
Helixi21 – 25Combined sources5
Beta strandi32 – 34Combined sources3
Beta strandi38 – 43Combined sources6
Turni44 – 46Combined sources3
Beta strandi47 – 50Combined sources4
Beta strandi52 – 55Combined sources4
Helixi61 – 68Combined sources8
Helixi70 – 76Combined sources7
Turni77 – 79Combined sources3
Beta strandi82 – 87Combined sources6
Helixi94 – 98Combined sources5
Turni99 – 101Combined sources3
Helixi104 – 106Combined sources3
Helixi114 – 128Combined sources15
Beta strandi132 – 144Combined sources13
Beta strandi147 – 150Combined sources4
Beta strandi163 – 166Combined sources4
Turni168 – 170Combined sources3
Beta strandi172 – 175Combined sources4
Beta strandi180 – 182Combined sources3
Helixi185 – 198Combined sources14
Beta strandi201 – 204Combined sources4
Beta strandi207 – 211Combined sources5
Beta strandi213 – 225Combined sources13
Beta strandi228 – 241Combined sources14
Helixi248 – 251Combined sources4
Helixi255 – 279Combined sources25
Helixi289 – 291Combined sources3
Helixi293 – 297Combined sources5
Turni298 – 303Combined sources6
Beta strandi304 – 314Combined sources11
Helixi318 – 320Combined sources3
Helixi321 – 334Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZFCX-ray1.80A1-344[»]
3ZFDX-ray1.71A1-344[»]
ProteinModelPortaliP33174.
SMRiP33174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 337Kinesin motorPROSITE-ProRule annotationAdd BLAST329

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1001 – 1231GlobularAdd BLAST231

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili351 – 1000Sequence analysisAdd BLAST650

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Chromokinesin subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0244. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133670.
HOGENOMiHOG000048108.
HOVERGENiHBG052256.
InParanoidiP33174.
KOiK10395.
OMAiDEEWIPT.
OrthoDBiEOG091G02I4.
TreeFamiTF105224.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
IPR033467. Tesmin/TSO1-like_CXC.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM01114. CXC. 1 hit.
SM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEEVKGIPV RVALRCRPLV SKEIKEGCQT CLSFVPGEPQ VVVGNDKSFT
60 70 80 90 100
YDFVFDPSTE QEEVFNTAVA PLIKGVFKGY NATVLAYGQT GSGKTYSMGG
110 120 130 140 150
AYTAEQEHDS AIGVIPRVIQ LLFKEINKKS DFEFTLKVSY LEIYNEEILD
160 170 180 190 200
LLCSSREKAT QINIREDPKE GIKIVGLTEK TVLVASDTVS CLEQGNNSRT
210 220 230 240 250
VASTAMNSQS SRSHAIFTIS IEQRKKNDKN SSFRSKLHLV DLAGSERQKK
260 270 280 290 300
TKAEGDRLRE GININRGLLC LGNVISALGD DKKGNFVPYR DSKLTRLLQD
310 320 330 340 350
SLGGNSHTLM IACVSPADSN LEETLNTLRY ADRARKIKNK PIINIDPQAA
360 370 380 390 400
ELNHLKQQVQ QLQILLLQAH GGTLPGDINV EPSENLQSLM EKNQSLVEEN
410 420 430 440 450
EKLSRGLSEA AGQTAQMLER IILTEQANEK MNAKLEELRR HAACKVDLQK
460 470 480 490 500
LVETLEDQEL KENIEIICNL QQVIAQLSDE AAACMTATID TAGEADTQVQ
510 520 530 540 550
SSPDTSRSSD VFSTQHALRQ AQMSKELIEL NKALALKEAL AKKMTQNDNQ
560 570 580 590 600
LQPIQFQYQD NIKNLESEVL SLQREKEELV LELQTAKKDA NQAKLSERRR
610 620 630 640 650
KRLQELEGQI ADLKKKLQEQ SKLLKLKEST EHTVSKLNQE IRMMKNQRVQ
660 670 680 690 700
LMRQMKEDAE KFRQWKQQKD KEVIQLKERD RKRQYELLKL ERNFQKQSNV
710 720 730 740 750
LRRKTEEAAA ANKRLKDALQ KQKEVAEKRK ETQSRGMEST AARMKNWLGN
760 770 780 790 800
EIEVMVSTEE AKRHLNGLLE ERKILAQDVA QLKEKRESGE NPPLKLRRRT
810 820 830 840 850
FSYDEIHGQD SGAEDSIAKQ IESLETELEL RSAQIADLQQ KLLDAESEDR
860 870 880 890 900
PKQRWESIAT ILEAKCAIKY LVGELVSSKI LVSKLESSLN QSKASCIDVQ
910 920 930 940 950
KMLFEEQNHF AKIETELKEE LVKVEQQHQE KVLYLLSQLQ QSQMTEKQLE
960 970 980 990 1000
ESVSEKEQQL LSTLKCQEEE LRKMQEVCEQ NQQLLQENSA IKQKLTLLQV
1010 1020 1030 1040 1050
ASKQKPHLTR NIFQSPDSSF EYIPPKPKPC RIKEKCLEQS FAVEGLQYYS
1060 1070 1080 1090 1100
EPSVAEQDNE DSDDHADEEW IPTKLVKVSK KSIQGCSCKG WCGNKQCGCR
1110 1120 1130 1140 1150
KQKSDCNVSC SCDPTKCRNR HQNQDNSDAI ELNQDSENSF KLEDPTEVTS
1160 1170 1180 1190 1200
GLSFFHPICA TPSSKILKEM CDADQVQLKQ PVFVSSSDHP ELKSIASESQ
1210 1220 1230
ENKAIGKKKK RALASNTSFF SGCSPIQEES H
Length:1,231
Mass (Da):139,519
Last modified:July 27, 2011 - v3
Checksum:iAA3C5E2E52678DE7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112I → S (PubMed:19468303).Curated1
Sequence conflicti1044 – 1045EG → GE in BAA02167 (PubMed:7929562).Curated2
Sequence conflicti1182V → M in BAA02167 (PubMed:7929562).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12646 mRNA. Translation: BAA02167.1.
BX005480, BX276129 Genomic DNA. Translation: CAM15067.1.
BX276129, BX005480 Genomic DNA. Translation: CAM17678.1.
CH466564 Genomic DNA. Translation: EDL14171.1.
BC050946 mRNA. Translation: AAH50946.1.
CCDSiCCDS30305.1.
PIRiA54803.
RefSeqiNP_032472.2. NM_008446.2.
UniGeneiMm.383091.

Genome annotation databases

EnsembliENSMUST00000048962; ENSMUSP00000048383; ENSMUSG00000034311.
GeneIDi16571.
KEGGimmu:16571.
UCSCiuc009twh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12646 mRNA. Translation: BAA02167.1.
BX005480, BX276129 Genomic DNA. Translation: CAM15067.1.
BX276129, BX005480 Genomic DNA. Translation: CAM17678.1.
CH466564 Genomic DNA. Translation: EDL14171.1.
BC050946 mRNA. Translation: AAH50946.1.
CCDSiCCDS30305.1.
PIRiA54803.
RefSeqiNP_032472.2. NM_008446.2.
UniGeneiMm.383091.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZFCX-ray1.80A1-344[»]
3ZFDX-ray1.71A1-344[»]
ProteinModelPortaliP33174.
SMRiP33174.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200944. 31 interactors.
IntActiP33174. 28 interactors.
STRINGi10090.ENSMUSP00000048383.

PTM databases

iPTMnetiP33174.
PhosphoSitePlusiP33174.

Proteomic databases

EPDiP33174.
MaxQBiP33174.
PaxDbiP33174.
PeptideAtlasiP33174.
PRIDEiP33174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048962; ENSMUSP00000048383; ENSMUSG00000034311.
GeneIDi16571.
KEGGimmu:16571.
UCSCiuc009twh.2. mouse.

Organism-specific databases

CTDi16571.
MGIiMGI:108389. Kif4.

Phylogenomic databases

eggNOGiKOG0244. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133670.
HOGENOMiHOG000048108.
HOVERGENiHBG052256.
InParanoidiP33174.
KOiK10395.
OMAiDEEWIPT.
OrthoDBiEOG091G02I4.
TreeFamiTF105224.

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-437239. Recycling pathway of L1.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-MMU-983189. Kinesins.

Miscellaneous databases

PROiP33174.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034311.
CleanExiMM_KIF4.
GenevisibleiP33174. MM.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
IPR033467. Tesmin/TSO1-like_CXC.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM01114. CXC. 1 hit.
SM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIF4_MOUSE
AccessioniPrimary (citable) accession number: P33174
Secondary accession number(s): Q80YP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.