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Protein

Elongation factor Tu

Gene

tuf

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi82 – 865GTPUniRule annotation
Nucleotide bindingi137 – 1404GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-HAMAP
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU01130-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Alternative name(s):
P-40
Gene namesi
Name:tufUniRule annotation
Synonyms:tufA
Ordered Locus Names:BSU01130
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU01130. [Micado]

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Elongation factor TuPRO_0000091291Add
BLAST

Proteomic databases

PaxDbiP33166.
PRIDEiP33166.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

DIPiDIP-52428N.
IntActiP33166. 4 interactions.
MINTiMINT-8364756.
STRINGi224308.BSU01130.

Structurei

3D structure databases

ProteinModelPortaliP33166.
SMRiP33166. Positions 9-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 205196tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 268G1By similarity
Regioni61 – 655G2By similarity
Regioni82 – 854G3By similarity
Regioni137 – 1404G4By similarity
Regioni175 – 1773G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
InParanoidiP33166.
KOiK02358.
OMAiDEGGRHN.
OrthoDBiEOG6R5C6X.
PhylomeDBiP33166.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEKFDRSK SHANIGTIGH VDHGKTTLTA AITTVLHKKS GKGTAMAYDQ
60 70 80 90 100
IDGAPEERER GITISTAHVE YETETRHYAH VDCPGHADYV KNMITGAAQM
110 120 130 140 150
DGAILVVSAA DGPMPQTREH ILLSKNVGVP YIVVFLNKCD MVDDEELLEL
160 170 180 190 200
VEMEVRDLLS EYDFPGDDVP VVKGSALKAL EGDAEWEAKI FELMDAVDEY
210 220 230 240 250
IPTPERDTEK PFMMPVEDVF SITGRGTVAT GRVERGQVKV GDEVEIIGLQ
260 270 280 290 300
EENKKTTVTG VEMFRKLLDY AEAGDNIGAL LRGVSREEIQ RGQVLAKPGT
310 320 330 340 350
ITPHSKFKAE VYVLSKEEGG RHTPFFSNYR PQFYFRTTDV TGIIHLPEGV
360 370 380 390
EMVMPGDNTE MNVELISTIA IEEGTRFSIR EGGRTVGSGV VSTITE
Length:396
Mass (Da):43,593
Last modified:October 1, 1993 - v1
Checksum:iBC096F47AAE6F72A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D64127 Genomic DNA. Translation: BAA11004.1.
AL009126 Genomic DNA. Translation: CAB11889.1.
PIRiA60663.
RefSeqiNP_387994.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB11889; CAB11889; BSU01130.
GeneIDi935965.
KEGGibsu:BSU01130.
PATRICi18971735. VBIBacSub10457_0116.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D64127 Genomic DNA. Translation: BAA11004.1.
AL009126 Genomic DNA. Translation: CAB11889.1.
PIRiA60663.
RefSeqiNP_387994.1. NC_000964.3.

3D structure databases

ProteinModelPortaliP33166.
SMRiP33166. Positions 9-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-52428N.
IntActiP33166. 4 interactions.
MINTiMINT-8364756.
STRINGi224308.BSU01130.

Proteomic databases

PaxDbiP33166.
PRIDEiP33166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11889; CAB11889; BSU01130.
GeneIDi935965.
KEGGibsu:BSU01130.
PATRICi18971735. VBIBacSub10457_0116.

Organism-specific databases

GenoListiBSU01130. [Micado]

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
InParanoidiP33166.
KOiK02358.
OMAiDEGGRHN.
OrthoDBiEOG6R5C6X.
PhylomeDBiP33166.

Enzyme and pathway databases

BioCyciBSUB:BSU01130-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequences of seven eubacterial genes coding for the elongation factor Tu: functional, structural and phylogenetic evaluations."
    Ludwig W., Weizenegger M., Betzl D., Leidel E., Lenz T., Ludvigsen A., Moellenhoff D., Wenzig P., Schleifer K.H.
    Arch. Microbiol. 153:241-247(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome."
    Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H.
    Microbiology 142:3039-3046(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiEFTU_BACSU
AccessioniPrimary (citable) accession number: P33166
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 4, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.