P33165 (EFTU_BACFR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Tu
Short name=EF-Tu

Gene names

Name:	tuf
Ordered Locus Names:	BF4199

Organism

Bacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]

Taxonomic identifier

295405 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides › Bacteroides fragilis

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118_B
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00118_B
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00118_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Elongation factor Tu HAMAP-Rule MF_00118_B		PRO_0000091288
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	136 – 139	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33165 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 7B4C6FD208323149	FASTA	394	43,580
	10 20 30 40 50 60 MAKEKFERTK PHVNIGTIGH VDHGKTTLTA AITTVLAKKG LSELRSFDSI DNAPEEKERG 70 80 90 100 110 120 ITINTSHVEY ETANRHYAHV DCPGHADYVK NMVTGAAQMD GAIIVVAATD GPMPQTREHI 130 140 150 160 170 180 LLARQVNVPK LVVFMNKCDM VEDAEMLELV EMEMRELLSF YDFDGDNTPI IQGSALGALN 190 200 210 220 230 240 GVEKWEDKVM ELMEAVDTWI PLPPRDVDKP FLMPVEDVFS ITGRGTVATG RIETGVIHVG 250 260 270 280 290 300 DEIEILGLGE DKKSVVTGVE MFRKLLDQGE AGDNVGLLLR GVDKNEIKRG MVLCKPGQIK 310 320 330 340 350 360 PHSKFKAEVY ILKKEEGGRH TPFHNKYRPQ FYLRTMDCTG EITLPEGTEM VMPGDNVTIT 370 380 390 VELIYPVALN IGLRFAIREG GRTVGAGQIT EIID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequences of seven eubacterial genes coding for the elongation factor Tu: functional, structural and phylogenetic evaluations." Ludwig W., Weizenegger M., Betzl D., Leidel E., Lenz T., Ludvigsen A., Moellenhoff D., Wenzig P., Schleifer K.H. Arch. Microbiol. 153:241-247(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006841 Genomic DNA. Translation: BAD50942.1.
PIR	B60663.
RefSeq	YP_101476.1. NC_006347.1.
3D structure databases
ProteinModelPortal	P33165.
SMR	P33165. Positions 2-393.
ModBase	Search...
Protein-protein interaction databases
STRING	295405.BF4199.
Proteomic databases
PRIDE	P33165.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD50942; BAD50942; BF4199.
GeneID	3085281.
KEGG	bfr:BF4199.
PATRIC	21053992. VBIBacFra17906_4054.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0050.
HOGENOM	HOG000229290.
KO	K02358.
OMA	THVEYES.
ProtClustDB	PRK12735.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF52540. SSF52540. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_BACFR

Accession

Primary (citable) accession number: P33165

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 29, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program