P33162 (ALR_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alanine racemase EC=5.1.1.1
Organism	Pseudomonas fluorescens
Taxonomic identifier	294 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	25 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Subunit structure	Homodimer.
Sequence similarities	Belongs to the alanine racemase family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.3. HAMAP MF_01201 Temperature dependence: Optimum temperature is 30 degrees Celsius. Thermolabile and psychrotrophic. Shows catalytic activity even at 0 degree Celsius.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›25	›25	Alanine racemase HAMAP MF_01201		PRO_0000114548
Experimental info
	Non-terminal residue	25	1

Sequences

	Sequence		Length	Mass (Da)
	P33162 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 54D51109F62B1A43	FASTA	25	2,775
	10 20 MXPARALIDL QALRHNYQLA AEVIG

References

[1]	"Thermolabile alanine racemase from a psychotroph, Pseudomonas fluorescens: purification and properties." Yokoigawa K., Kawai H., Endo K., Lim Y.H., Esaki N., Soda K. Biosci. Biotechnol. Biochem. 57:93-97(1993) [PubMed: 7763424] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: TM5-2.

Cross-references

Sequence databases
PIR	PC1221.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
PROSITE	PS00395. ALANINE_RACEMASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_PSEFL

Accession

Primary (citable) accession number: P33162

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 31, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program