Reviewed, UniProtKB/Swiss-Prot P33157 (E13A_ARATH)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-beta-glucosidase, acidic isoform
    EC=3.2.1.39
Alternative name(s):
    (1->3)-beta-glucan endohydrolase
      Short name=(1->3)-beta-glucanase
    Beta-1,3-endoglucanase
    Pathogenesis-related protein 2
      Short name=PR-2
    Beta-1,3-glucanase 2

Gene names

Name:	BG2
Ordered Locus Names:	At3g57260
ORF Names:	F28O9.110

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted › extracellular space › apoplast. Note: Accumulates in the apoplast before secretion.
Induction	By 2,6-dichloroisonicotinic acid (INA) and salicylic acid (possibly an endogenous signal for acquired resistance). Strongly induced by pathogen infection.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.
Sequence caution	The sequence AAA32755.1 differs from that shown. Reason: Frameshift at position 62. The sequence AAA32864.1 differs from that shown. Reason: Frameshift at position 62.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Pathogenesis-related protein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro response to cold Inferred from expression pattern. Source: TAIR systemic acquired resistance Ref.1 Inferred from expression pattern. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR cell wall Inferred from direct assay. Source: TAIR vacuole Inferred from direct assay. Source: TAIR
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro cellulase activity Traceable author statement. Source: TAIR glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 339	309	Glucan endo-1,3-beta-glucosidase, acidic isoform		PRO_0000011882
Sites
	Active site	263	1	Nucleophile By similarity
	Active site	320	1	Proton donor By similarity
Experimental info
	Sequence conflict	27	1	H → P Ref.2
	Sequence conflict	27	1	H → P Ref.5
	Sequence conflict	332	1	S → P in AAM63339. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P33157-1 [UniParc]. Last modified December 6, 2002. Version 2. Checksum: 3B145DB01733BD22	FASTA	339	37,339
	10 20 30 40 50 60 MSESRSLASP PMLMILLSLV IASFFNHTAG QIGVCYGMLG DTLPSPSDVV ALYKQQNIQR 70 80 90 100 110 120 MRLYGPDPGA LAALRGSDIE LILDVPSSDL ERLASSQTEA DKWVQENVQS YRDGVRFRYI 130 140 150 160 170 180 NVGNEVKPSV GGFLLQAMQN IENAVSGAGL EVKVSTAIAT DTTTDTSPPS QGRFRDEYKS 190 200 210 220 230 240 FLEPVIGFLA SKQSPLLVNL YPYFSYMGDT ANIHLDYALF TAQSTVDNDP GYSYQNLFDA 250 260 270 280 290 300 NLDSVYAALE KSGGGSLEIV VSETGWPTEG AVGTSVENAK TYVNNLIQHV KNGSPRRPGK 310 320 330 AIETYIFAMF DENKKEPTYE KFWGLFHPDR QSKYEVNFN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Acquired resistance in Arabidopsis." Uknes S., Mauch-Mani B., Moyer M., Potter S., Williams S., Dincher S., Chandler D., Slusarenko A., Ward E., Ryals J. Plant Cell 4:645-656(1992) [PubMed: 1392589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Landsberg erecta. Tissue: Leaf.
[2]	"Induction of Arabidopsis defense genes by virulent and avirulent Pseudomonas syringae strains and by a cloned avirulence gene." Dong X., Mindrinos M., Davis K., Ausubel F. Plant Cell 3:61-72(1991) [PubMed: 1824335] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M90509 mRNA. Translation: AAA32864.1. Frameshift. M58462 Genomic DNA. Translation: AAA32755.1. Frameshift. AL137080 Genomic DNA. Translation: CAB68132.1. AY099668 mRNA. Translation: AAM20519.1. AY128847 mRNA. Translation: AAM91247.1. AY086134 mRNA. Translation: AAM63339.1.
IPI	IPI00547460.
PIR	JQ1694. T45804.
RefSeq	NP_191285.1.
UniGene	At.22313
3D structure databases
SMR	P33157. Positions 32-338.
ModBase	Search...
Protein-protein interaction databases
STRING	P33157.
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Proteomic databases
PRIDE	P33157.
Genome annotation databases
GeneID	824893.
GenomeReviews	Gene locus AT3G57260 in contig BA000014_GR.
KEGG	ath:AT3G57260.
NMPDR	fig|3702.1.peg.17058.
Organism-specific databases
TAIR	At3g57260.
Phylogenomic databases
HOGENOM	HBG605194.
InParanoid	P33157.
OMA	NIHAMRI.
Enzyme and pathway databases
BRENDA	3.2.1.39. 302.
Gene expression databases
ArrayExpress	P33157.
Genevestigator	P33157.
GermOnline	AT3G57260. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13A_ARATH

Accession

Primary (citable) accession number: P33157
Secondary accession number(s): Q8LD94, Q9M2M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	December 6, 2002
Last modified:	February 9, 2010
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)