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P33157

- E13A_ARATH

UniProt

P33157 - E13A_ARATH

Protein

Glucan endo-1,3-beta-glucosidase, acidic isoform

Gene

BG2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (06 Dec 2002)
      Previous versions | rss
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    Functioni

    Implicated in the defense of plants against pathogens.

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei263 – 2631NucleophileBy similarity
    Active sitei320 – 3201Proton donorBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: TAIR
    2. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC
    3. glucan exo-1,3-beta-glucosidase activity Source: TAIR
    4. protein binding Source: TAIR

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. response to cold Source: TAIR
    3. systemic acquired resistance Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Pathogenesis-related protein

    Keywords - Biological processi

    Plant defense

    Enzyme and pathway databases

    BioCyciARA:AT3G57260-MONOMER.

    Protein family/group databases

    CAZyiGH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase, acidic isoform (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase
    Short name:
    (1->3)-beta-glucanase
    Beta-1,3-endoglucanase
    Beta-1,3-glucanase 2
    Pathogenesis-related protein 2
    Short name:
    PR-2
    Gene namesi
    Name:BG2
    Ordered Locus Names:At3g57260
    ORF Names:F28O9.110
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G57260.

    Subcellular locationi

    Secretedextracellular spaceapoplast
    Note: Accumulates in the apoplast before secretion.

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. cell wall Source: TAIR
    3. vacuole Source: TAIR

    Keywords - Cellular componenti

    Apoplast, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Sequence AnalysisAdd
    BLAST
    Chaini31 – 339309Glucan endo-1,3-beta-glucosidase, acidic isoformPRO_0000011882Add
    BLAST

    Proteomic databases

    PaxDbiP33157.
    PRIDEiP33157.

    Expressioni

    Inductioni

    By 2,6-dichloroisonicotinic acid (INA) and salicylic acid (possibly an endogenous signal for acquired resistance). Strongly induced by pathogen infection.

    Gene expression databases

    ArrayExpressiP33157.
    GenevestigatoriP33157.

    Interactioni

    Protein-protein interaction databases

    IntActiP33157. 1 interaction.
    STRINGi3702.AT3G57260.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP33157.
    SMRiP33157. Positions 31-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG268889.
    HOGENOMiHOG000238220.
    InParanoidiP33157.
    OMAiFRYINVG.
    PhylomeDBiP33157.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33157-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSESRSLASP PMLMILLSLV IASFFNHTAG QIGVCYGMLG DTLPSPSDVV    50
    ALYKQQNIQR MRLYGPDPGA LAALRGSDIE LILDVPSSDL ERLASSQTEA 100
    DKWVQENVQS YRDGVRFRYI NVGNEVKPSV GGFLLQAMQN IENAVSGAGL 150
    EVKVSTAIAT DTTTDTSPPS QGRFRDEYKS FLEPVIGFLA SKQSPLLVNL 200
    YPYFSYMGDT ANIHLDYALF TAQSTVDNDP GYSYQNLFDA NLDSVYAALE 250
    KSGGGSLEIV VSETGWPTEG AVGTSVENAK TYVNNLIQHV KNGSPRRPGK 300
    AIETYIFAMF DENKKEPTYE KFWGLFHPDR QSKYEVNFN 339
    Length:339
    Mass (Da):37,339
    Last modified:December 6, 2002 - v2
    Checksum:i3B145DB01733BD22
    GO

    Sequence cautioni

    The sequence AAA32755.1 differs from that shown. Reason: Frameshift at position 62.
    The sequence AAA32864.1 differs from that shown. Reason: Frameshift at position 62.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti27 – 271H → P(PubMed:1824335)Curated
    Sequence conflicti27 – 271H → P1 PublicationCurated
    Sequence conflicti332 – 3321S → P in AAM63339. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90509 mRNA. Translation: AAA32864.1. Frameshift.
    M58462 Genomic DNA. Translation: AAA32755.1. Frameshift.
    AL137080 Genomic DNA. Translation: CAB68132.1.
    CP002686 Genomic DNA. Translation: AEE79633.1.
    AY099668 mRNA. Translation: AAM20519.1.
    AY128847 mRNA. Translation: AAM91247.1.
    AY086134 mRNA. Translation: AAM63339.1.
    PIRiJQ1694.
    T45804.
    RefSeqiNP_191285.1. NM_115586.2.
    UniGeneiAt.22313.

    Genome annotation databases

    EnsemblPlantsiAT3G57260.1; AT3G57260.1; AT3G57260.
    GeneIDi824893.
    KEGGiath:AT3G57260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90509 mRNA. Translation: AAA32864.1 . Frameshift.
    M58462 Genomic DNA. Translation: AAA32755.1 . Frameshift.
    AL137080 Genomic DNA. Translation: CAB68132.1 .
    CP002686 Genomic DNA. Translation: AEE79633.1 .
    AY099668 mRNA. Translation: AAM20519.1 .
    AY128847 mRNA. Translation: AAM91247.1 .
    AY086134 mRNA. Translation: AAM63339.1 .
    PIRi JQ1694.
    T45804.
    RefSeqi NP_191285.1. NM_115586.2.
    UniGenei At.22313.

    3D structure databases

    ProteinModelPortali P33157.
    SMRi P33157. Positions 31-339.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P33157. 1 interaction.
    STRINGi 3702.AT3G57260.1-P.

    Protein family/group databases

    CAZyi GH17. Glycoside Hydrolase Family 17.

    Proteomic databases

    PaxDbi P33157.
    PRIDEi P33157.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G57260.1 ; AT3G57260.1 ; AT3G57260 .
    GeneIDi 824893.
    KEGGi ath:AT3G57260.

    Organism-specific databases

    TAIRi AT3G57260.

    Phylogenomic databases

    eggNOGi NOG268889.
    HOGENOMi HOG000238220.
    InParanoidi P33157.
    OMAi FRYINVG.
    PhylomeDBi P33157.

    Enzyme and pathway databases

    BioCyci ARA:AT3G57260-MONOMER.

    Gene expression databases

    ArrayExpressi P33157.
    Genevestigatori P33157.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: cv. Landsberg erecta.
      Tissue: Leaf.
    2. "Induction of Arabidopsis defense genes by virulent and avirulent Pseudomonas syringae strains and by a cloned avirulence gene."
      Dong X., Mindrinos M., Davis K., Ausubel F.
      Plant Cell 3:61-72(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiE13A_ARATH
    AccessioniPrimary (citable) accession number: P33157
    Secondary accession number(s): Q8LD94, Q9M2M1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: December 6, 2002
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3