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P33157

- E13A_ARATH

UniProt

P33157 - E13A_ARATH

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Protein

Glucan endo-1,3-beta-glucosidase, acidic isoform

Gene

BG2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Implicated in the defense of plants against pathogens.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei263 – 2631NucleophileBy similarity
Active sitei320 – 3201Proton donorBy similarity

GO - Molecular functioni

  1. cellulase activity Source: TAIR
  2. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC
  3. glucan exo-1,3-beta-glucosidase activity Source: TAIR

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. response to cold Source: TAIR
  3. systemic acquired resistance Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Pathogenesis-related protein

Keywords - Biological processi

Plant defense

Enzyme and pathway databases

BioCyciARA:AT3G57260-MONOMER.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase, acidic isoform (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase
Beta-1,3-glucanase 2
Pathogenesis-related protein 2
Short name:
PR-2
Gene namesi
Name:BG2
Ordered Locus Names:At3g57260
ORF Names:F28O9.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G57260.

Subcellular locationi

Secretedextracellular spaceapoplast
Note: Accumulates in the apoplast before secretion.

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. cell wall Source: TAIR
  3. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 339309Glucan endo-1,3-beta-glucosidase, acidic isoformPRO_0000011882Add
BLAST

Proteomic databases

PaxDbiP33157.
PRIDEiP33157.

Expressioni

Inductioni

By 2,6-dichloroisonicotinic acid (INA) and salicylic acid (possibly an endogenous signal for acquired resistance). Strongly induced by pathogen infection.

Gene expression databases

ExpressionAtlasiP33157. baseline and differential.
GenevestigatoriP33157.

Interactioni

Protein-protein interaction databases

IntActiP33157. 1 interaction.
STRINGi3702.AT3G57260.1-P.

Structurei

3D structure databases

ProteinModelPortaliP33157.
SMRiP33157. Positions 31-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG268889.
HOGENOMiHOG000238220.
InParanoidiP33157.
OMAiFRYINVG.
PhylomeDBiP33157.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33157-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSESRSLASP PMLMILLSLV IASFFNHTAG QIGVCYGMLG DTLPSPSDVV
60 70 80 90 100
ALYKQQNIQR MRLYGPDPGA LAALRGSDIE LILDVPSSDL ERLASSQTEA
110 120 130 140 150
DKWVQENVQS YRDGVRFRYI NVGNEVKPSV GGFLLQAMQN IENAVSGAGL
160 170 180 190 200
EVKVSTAIAT DTTTDTSPPS QGRFRDEYKS FLEPVIGFLA SKQSPLLVNL
210 220 230 240 250
YPYFSYMGDT ANIHLDYALF TAQSTVDNDP GYSYQNLFDA NLDSVYAALE
260 270 280 290 300
KSGGGSLEIV VSETGWPTEG AVGTSVENAK TYVNNLIQHV KNGSPRRPGK
310 320 330
AIETYIFAMF DENKKEPTYE KFWGLFHPDR QSKYEVNFN
Length:339
Mass (Da):37,339
Last modified:December 6, 2002 - v2
Checksum:i3B145DB01733BD22
GO

Sequence cautioni

The sequence AAA32755.1 differs from that shown. Reason: Frameshift at position 62. Curated
The sequence AAA32864.1 differs from that shown. Reason: Frameshift at position 62. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271H → P(PubMed:1824335)Curated
Sequence conflicti27 – 271H → P1 PublicationCurated
Sequence conflicti332 – 3321S → P in AAM63339. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90509 mRNA. Translation: AAA32864.1. Frameshift.
M58462 Genomic DNA. Translation: AAA32755.1. Frameshift.
AL137080 Genomic DNA. Translation: CAB68132.1.
CP002686 Genomic DNA. Translation: AEE79633.1.
AY099668 mRNA. Translation: AAM20519.1.
AY128847 mRNA. Translation: AAM91247.1.
AY086134 mRNA. Translation: AAM63339.1.
PIRiJQ1694.
T45804.
RefSeqiNP_191285.1. NM_115586.2.
UniGeneiAt.22313.

Genome annotation databases

EnsemblPlantsiAT3G57260.1; AT3G57260.1; AT3G57260.
GeneIDi824893.
KEGGiath:AT3G57260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90509 mRNA. Translation: AAA32864.1 . Frameshift.
M58462 Genomic DNA. Translation: AAA32755.1 . Frameshift.
AL137080 Genomic DNA. Translation: CAB68132.1 .
CP002686 Genomic DNA. Translation: AEE79633.1 .
AY099668 mRNA. Translation: AAM20519.1 .
AY128847 mRNA. Translation: AAM91247.1 .
AY086134 mRNA. Translation: AAM63339.1 .
PIRi JQ1694.
T45804.
RefSeqi NP_191285.1. NM_115586.2.
UniGenei At.22313.

3D structure databases

ProteinModelPortali P33157.
SMRi P33157. Positions 31-339.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P33157. 1 interaction.
STRINGi 3702.AT3G57260.1-P.

Protein family/group databases

CAZyi GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PaxDbi P33157.
PRIDEi P33157.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G57260.1 ; AT3G57260.1 ; AT3G57260 .
GeneIDi 824893.
KEGGi ath:AT3G57260.

Organism-specific databases

TAIRi AT3G57260.

Phylogenomic databases

eggNOGi NOG268889.
HOGENOMi HOG000238220.
InParanoidi P33157.
OMAi FRYINVG.
PhylomeDBi P33157.

Enzyme and pathway databases

BioCyci ARA:AT3G57260-MONOMER.

Gene expression databases

ExpressionAtlasi P33157. baseline and differential.
Genevestigatori P33157.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Landsberg erecta.
    Tissue: Leaf.
  2. "Induction of Arabidopsis defense genes by virulent and avirulent Pseudomonas syringae strains and by a cloned avirulence gene."
    Dong X., Mindrinos M., Davis K., Ausubel F.
    Plant Cell 3:61-72(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiE13A_ARATH
AccessioniPrimary (citable) accession number: P33157
Secondary accession number(s): Q8LD94, Q9M2M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 6, 2002
Last modified: October 29, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3