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P33151

- CADH5_HUMAN

UniProt

P33151 - CADH5_HUMAN

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Protein

Cadherin-5

Gene

CDH5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi58 – 581Calcium 1By similarity
Metal bindingi58 – 581Calcium 2By similarity
Metal bindingi59 – 591Calcium 1By similarity
Metal bindingi109 – 1091Calcium 1By similarity
Metal bindingi111 – 1111Calcium 1By similarity
Metal bindingi111 – 1111Calcium 2By similarity
Metal bindingi143 – 1431Calcium 2By similarity
Metal bindingi144 – 1441Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi145 – 1451Calcium 3By similarity
Metal bindingi146 – 1461Calcium 1By similarity
Metal bindingi146 – 1461Calcium 2By similarity
Metal bindingi147 – 1471Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi177 – 1771Calcium 3By similarity
Metal bindingi179 – 1791Calcium 2By similarity
Metal bindingi179 – 1791Calcium 3By similarity
Metal bindingi186 – 1861Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi231 – 2311Calcium 3By similarity

GO - Molecular functioni

  1. beta-catenin binding Source: BHF-UCL
  2. calcium ion binding Source: InterPro
  3. ion channel binding Source: BHF-UCL
  4. receptor binding Source: BHF-UCL

GO - Biological processi

  1. adherens junction organization Source: Reactome
  2. blood vessel maturation Source: Ensembl
  3. cell-cell junction organization Source: Reactome
  4. cell junction assembly Source: Reactome
  5. homophilic cell adhesion via plasma membrane adhesion molecules Source: ProtInc
  6. negative regulation of cell proliferation Source: Ensembl
  7. regulation of establishment of cell polarity Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_19195. Adherens junctions interactions.
REACT_228016. VEGFR2 mediated vascular permeability.

Names & Taxonomyi

Protein namesi
Recommended name:
Cadherin-5
Alternative name(s):
7B4 antigen
Vascular endothelial cadherin
Short name:
VE-cadherin
CD_antigen: CD144
Gene namesi
Name:CDH5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:1764. CDH5.

Subcellular locationi

Cell junction 1 Publication. Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication
Note: Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini48 – 599552ExtracellularSequence AnalysisAdd
BLAST
Transmembranei600 – 62021HelicalSequence AnalysisAdd
BLAST
Topological domaini621 – 784164CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell-cell junction Source: UniProtKB
  2. cell junction Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
  4. membrane Source: ProtInc
  5. plasma membrane Source: Reactome
  6. tight junction Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26301.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Propeptidei26 – 4722Sequence AnalysisPRO_0000003755Add
BLAST
Chaini48 – 784737Cadherin-5PRO_0000003756Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...) (complex)2 Publications
Glycosylationi112 – 1121N-linked (GlcNAc...) (complex)3 Publications
Glycosylationi157 – 1571N-linked (GlcNAc...)1 Publication
Glycosylationi362 – 3621N-linked (GlcNAc...)1 Publication
Glycosylationi442 – 4421N-linked (GlcNAc...) (complex)3 Publications
Glycosylationi523 – 5231N-linked (GlcNAc...)1 Publication
Glycosylationi535 – 5351N-linked (GlcNAc...)1 Publication

Post-translational modificationi

Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB (By similarity).By similarity
O-glycosylated.3 Publications

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP33151.
PaxDbiP33151.
PRIDEiP33151.

PTM databases

PhosphoSiteiP33151.
UniCarbKBiP33151.

Miscellaneous databases

PMAP-CutDBP33151.

Expressioni

Tissue specificityi

Endothelial tissues and brain.

Gene expression databases

BgeeiP33151.
CleanExiHS_CDH5.
ExpressionAtlasiP33151. baseline and differential.
GenevestigatoriP33151.

Organism-specific databases

HPAiCAB028366.
HPA030562.

Interactioni

Subunit structurei

Interacts (via cadherin 5 domain) with PTPRB (By similarity). Interacts with TRPC4. Interacts with KRIT1. Interacts with PARD3 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CTNNB1P352226EBI-2903122,EBI-491549
PARD3Q8TEW05EBI-2903122,EBI-81968
PARD6AQ9NPB64EBI-2903122,EBI-81876

Protein-protein interaction databases

BioGridi107438. 13 interactions.
DIPiDIP-41172N.
IntActiP33151. 10 interactions.
MINTiMINT-235470.
STRINGi9606.ENSP00000344115.

Structurei

3D structure databases

ProteinModelPortaliP33151.
SMRiP33151. Positions 48-576, 679-775.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 151104Cadherin 1PROSITE-ProRule annotationAdd
BLAST
Domaini152 – 258107Cadherin 2PROSITE-ProRule annotationAdd
BLAST
Domaini259 – 372114Cadherin 3PROSITE-ProRule annotationAdd
BLAST
Domaini373 – 477105Cadherin 4PROSITE-ProRule annotationAdd
BLAST
Domaini478 – 593116Cadherin 5PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi736 – 75318Ser-richAdd
BLAST

Domaini

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.By similarity

Sequence similaritiesi

Contains 5 cadherin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG270902.
GeneTreeiENSGT00760000118954.
HOVERGENiHBG005217.
InParanoidiP33151.
KOiK06533.
OMAiYGYEGSE.
OrthoDBiEOG773XFF.
PhylomeDBiP33151.
TreeFamiTF329887.

Family and domain databases

Gene3Di2.60.40.60. 5 hits.
4.10.900.10. 1 hit.
InterProiIPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR000233. Cadherin_cytoplasmic-dom.
IPR027397. Catenin_binding_dom.
[Graphical view]
PfamiPF00028. Cadherin. 5 hits.
PF01049. Cadherin_C. 1 hit.
[Graphical view]
PRINTSiPR00205. CADHERIN.
SMARTiSM00112. CA. 5 hits.
[Graphical view]
SUPFAMiSSF49313. SSF49313. 5 hits.
PROSITEiPS00232. CADHERIN_1. 3 hits.
PS50268. CADHERIN_2. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P33151-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQRLMMLLAT SGACLGLLAV AAVAAAGANP AQRDTHSLLP THRRQKRDWI
60 70 80 90 100
WNQMHIDEEK NTSLPHHVGK IKSSVSRKNA KYLLKGEYVG KVFRVDAETG
110 120 130 140 150
DVFAIERLDR ENISEYHLTA VIVDKDTGEN LETPSSFTIK VHDVNDNWPV
160 170 180 190 200
FTHRLFNASV PESSAVGTSV ISVTAVDADD PTVGDHASVM YQILKGKEYF
210 220 230 240 250
AIDNSGRIIT ITKSLDREKQ ARYEIVVEAR DAQGLRGDSG TATVLVTLQD
260 270 280 290 300
INDNFPFFTQ TKYTFVVPED TRVGTSVGSL FVEDPDEPQN RMTKYSILRG
310 320 330 340 350
DYQDAFTIET NPAHNEGIIK PMKPLDYEYI QQYSFIVEAT DPTIDLRYMS
360 370 380 390 400
PPAGNRAQVI INITDVDEPP IFQQPFYHFQ LKENQKKPLI GTVLAMDPDA
410 420 430 440 450
ARHSIGYSIR RTSDKGQFFR VTKKGDIYNE KELDREVYPW YNLTVEAKEL
460 470 480 490 500
DSTGTPTGKE SIVQVHIEVL DENDNAPEFA KPYQPKVCEN AVHGQLVLQI
510 520 530 540 550
SAIDKDITPR NVKFKFILNT ENNFTLTDNH DNTANITVKY GQFDREHTKV
560 570 580 590 600
HFLPVVISDN GMPSRTGTST LTVAVCKCNE QGEFTFCEDM AAQVGVSIQA
610 620 630 640 650
VVAILLCILT ITVITLLIFL RRRLRKQARA HGKSVPEIHE QLVTYDEEGG
660 670 680 690 700
GEMDTTSYDV SVLNSVRRGG AKPPRPALDA RPSLYAQVQK PPRHAPGAHG
710 720 730 740 750
GPGEMAAMIE VKKDEADHDG DGPPYDTLHI YGYEGSESIA ESLSSLGTDS
760 770 780
SDSDVDYDFL NDWGPRFKML AELYGSDPRE ELLY
Length:784
Mass (Da):87,528
Last modified:February 8, 2011 - v5
Checksum:i6DBEBFC4DA6899D1
GO
Isoform 2 (identifier: P33151-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     380-494: Missing.

Note: No experimental confirmation available.

Show »
Length:669
Mass (Da):74,499
Checksum:i379A177E6E279C9A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti602 – 6021V → A in AAH96363. (PubMed:15489334)Curated
Sequence conflicti602 – 6021V → A in AAI17521. (PubMed:15489334)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti503 – 5031I → T.
Corresponds to variant rs16956504 [ dbSNP | Ensembl ].
VAR_028003
Natural varianti517 – 5171I → T.3 Publications
Corresponds to variant rs1049970 [ dbSNP | Ensembl ].
VAR_028004

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei380 – 494115Missing in isoform 2. 1 PublicationVSP_053861Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79981 mRNA. Translation: CAA56306.1.
U84722 mRNA. Translation: AAB41796.1.
AB035304 mRNA. Translation: BAA87418.1.
BC096363 mRNA. Translation: AAH96363.1.
BC096364 mRNA. Translation: AAH96364.3.
BC117520 mRNA. Translation: AAI17521.1.
X59796 mRNA. Translation: CAA42468.1.
CCDSiCCDS10804.1. [P33151-1]
PIRiS49893. IJHUC5.
RefSeqiNP_001786.2. NM_001795.3. [P33151-1]
UniGeneiHs.76206.

Genome annotation databases

EnsembliENST00000341529; ENSP00000344115; ENSG00000179776. [P33151-1]
ENST00000614547; ENSP00000479381; ENSG00000179776. [P33151-2]
GeneIDi1003.
KEGGihsa:1003.
UCSCiuc002eom.4. human. [P33151-1]

Polymorphism databases

DMDMi322510142.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79981 mRNA. Translation: CAA56306.1 .
U84722 mRNA. Translation: AAB41796.1 .
AB035304 mRNA. Translation: BAA87418.1 .
BC096363 mRNA. Translation: AAH96363.1 .
BC096364 mRNA. Translation: AAH96364.3 .
BC117520 mRNA. Translation: AAI17521.1 .
X59796 mRNA. Translation: CAA42468.1 .
CCDSi CCDS10804.1. [P33151-1 ]
PIRi S49893. IJHUC5.
RefSeqi NP_001786.2. NM_001795.3. [P33151-1 ]
UniGenei Hs.76206.

3D structure databases

ProteinModelPortali P33151.
SMRi P33151. Positions 48-576, 679-775.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107438. 13 interactions.
DIPi DIP-41172N.
IntActi P33151. 10 interactions.
MINTi MINT-235470.
STRINGi 9606.ENSP00000344115.

Chemistry

DrugBanki DB00480. Lenalidomide.

PTM databases

PhosphoSitei P33151.
UniCarbKBi P33151.

Polymorphism databases

DMDMi 322510142.

Proteomic databases

MaxQBi P33151.
PaxDbi P33151.
PRIDEi P33151.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000341529 ; ENSP00000344115 ; ENSG00000179776 . [P33151-1 ]
ENST00000614547 ; ENSP00000479381 ; ENSG00000179776 . [P33151-2 ]
GeneIDi 1003.
KEGGi hsa:1003.
UCSCi uc002eom.4. human. [P33151-1 ]

Organism-specific databases

CTDi 1003.
GeneCardsi GC16P066400.
H-InvDB HIX0038527.
HGNCi HGNC:1764. CDH5.
HPAi CAB028366.
HPA030562.
MIMi 601120. gene.
neXtProti NX_P33151.
PharmGKBi PA26301.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG270902.
GeneTreei ENSGT00760000118954.
HOVERGENi HBG005217.
InParanoidi P33151.
KOi K06533.
OMAi YGYEGSE.
OrthoDBi EOG773XFF.
PhylomeDBi P33151.
TreeFami TF329887.

Enzyme and pathway databases

Reactomei REACT_19195. Adherens junctions interactions.
REACT_228016. VEGFR2 mediated vascular permeability.

Miscellaneous databases

ChiTaRSi CDH5. human.
GeneWikii VE-cadherin.
GenomeRNAii 1003.
NextBioi 4216.
PMAP-CutDB P33151.
PROi P33151.
SOURCEi Search...

Gene expression databases

Bgeei P33151.
CleanExi HS_CDH5.
ExpressionAtlasi P33151. baseline and differential.
Genevestigatori P33151.

Family and domain databases

Gene3Di 2.60.40.60. 5 hits.
4.10.900.10. 1 hit.
InterProi IPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
IPR000233. Cadherin_cytoplasmic-dom.
IPR027397. Catenin_binding_dom.
[Graphical view ]
Pfami PF00028. Cadherin. 5 hits.
PF01049. Cadherin_C. 1 hit.
[Graphical view ]
PRINTSi PR00205. CADHERIN.
SMARTi SM00112. CA. 5 hits.
[Graphical view ]
SUPFAMi SSF49313. SSF49313. 5 hits.
PROSITEi PS00232. CADHERIN_1. 3 hits.
PS50268. CADHERIN_2. 5 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional properties of human vascular endothelial cadherin (7B4/cadherin-5), an endothelium-specific cadherin."
    Breviario F., Caveda L., Corada M., Martin-Padura I., Navarro P., Golay J., Introna M., Gulino D., Lampugnani M.G., Dejana E.
    Arterioscler. Thromb. Vasc. Biol. 15:1229-1239(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT THR-517.
    Tissue: Endothelial cell.
  2. "Vascular endothelial cadherin (VE-cadherin): cloning and role in endothelial cell-cell adhesion."
    Ali J., Liao F., Martens E., Muller W.A.
    Microcirculation 4:267-277(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Placenta.
  3. "Identification of three human type-II classic cadherins and frequent heterophilic interactions between different subclasses of type-II classic cadherins."
    Shimoyama Y., Tsujimoto G., Kitajima M., Natori M.
    Biochem. J. 349:159-167(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT THR-517.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-517.
  5. "Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue."
    Suzuki S., Sano K., Tanihara H.
    Cell Regul. 2:261-270(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-784 (ISOFORM 1).
    Tissue: Brain.
  6. "A novel endothelial-specific membrane protein is a marker of cell-cell contacts."
    Lampugnani M.G., Resnati M., Raiteri M., Pigott R., Pisacane A., Houen G., Ruco L.P., Dejana E.
    J. Cell Biol. 118:1511-1522(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Tissue: Endothelial cell.
  7. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
    Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
    J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-112; ASN-442; ASN-523 AND ASN-535.
    Tissue: Plasma.
  8. Cited for: GLYCOSYLATION AT ASN-61; ASN-112 AND ASN-442.
  9. "Cell-cell contact formation governs Ca2+ signaling by TRPC4 in the vascular endothelium: evidence for a regulatory TRPC4-beta-catenin interaction."
    Graziani A., Poteser M., Heupel W.M., Schleifer H., Krenn M., Drenckhahn D., Romanin C., Baumgartner W., Groschner K.
    J. Biol. Chem. 285:4213-4223(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRPC4.
  10. "CCM1 regulates vascular-lumen organization by inducing endothelial polarity."
    Lampugnani M.G., Orsenigo F., Rudini N., Maddaluno L., Boulday G., Chapon F., Dejana E.
    J. Cell Sci. 123:1073-1080(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KRIT1.
  11. "Structure and binding mechanism of vascular endothelial cadherin: a divergent classical cadherin."
    Brasch J., Harrison O.J., Ahlsen G., Carnally S.M., Henderson R.M., Honig B., Shapiro L.
    J. Mol. Biol. 408:57-73(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GLYCOSYLATION AT ASN-61; ASN-112; ASN-157; ASN-362 AND ASN-442.

Entry informationi

Entry nameiCADH5_HUMAN
AccessioniPrimary (citable) accession number: P33151
Secondary accession number(s): Q4VAI5, Q4VAI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 8, 2011
Last modified: November 26, 2014
This is version 136 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3