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Protein

Glucans biosynthesis protein G

Gene

mdoG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of osmoregulated periplasmic glucans (OPGs).

Pathwayi: osmoregulated periplasmic glucan (OPG) biosynthesis

This protein is involved in the pathway osmoregulated periplasmic glucan (OPG) biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway osmoregulated periplasmic glucan (OPG) biosynthesis and in Glycan metabolism.

GO - Molecular functioni

GO - Biological processi

  • beta-glucan biosynthetic process Source: EcoCyc
  • response to osmotic stress Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG11885-MONOMER.
ECOL316407:JW1035-MONOMER.
UniPathwayiUPA00637.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucans biosynthesis protein G
Gene namesi
Name:mdoG
Synonyms:opgG
Ordered Locus Names:b1048, JW1035
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11885. mdoG.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22221 PublicationAdd
BLAST
Chaini23 – 511489Glucans biosynthesis protein GPRO_0000020221Add
BLAST

Proteomic databases

EPDiP33136.
PaxDbiP33136.
PRIDEiP33136.

2D gel databases

SWISS-2DPAGEP33136.

Interactioni

Protein-protein interaction databases

BioGridi4260686. 329 interactions.
DIPiDIP-10177N.
IntActiP33136. 6 interactions.
STRINGi511145.b1048.

Structurei

Secondary structure

1
511
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi25 – 3612Combined sources
Helixi56 – 594Combined sources
Helixi66 – 683Combined sources
Turni71 – 733Combined sources
Beta strandi79 – 846Combined sources
Beta strandi93 – 986Combined sources
Beta strandi103 – 1053Combined sources
Helixi110 – 1123Combined sources
Turni122 – 1243Combined sources
Beta strandi130 – 1378Combined sources
Beta strandi146 – 1538Combined sources
Beta strandi156 – 1594Combined sources
Beta strandi169 – 1724Combined sources
Beta strandi174 – 1763Combined sources
Beta strandi187 – 1948Combined sources
Beta strandi203 – 2119Combined sources
Beta strandi214 – 22310Combined sources
Beta strandi225 – 24016Combined sources
Beta strandi245 – 2528Combined sources
Beta strandi254 – 2574Combined sources
Beta strandi271 – 2733Combined sources
Beta strandi276 – 2805Combined sources
Beta strandi286 – 2905Combined sources
Beta strandi298 – 3069Combined sources
Beta strandi309 – 3135Combined sources
Helixi319 – 3213Combined sources
Turni325 – 3273Combined sources
Helixi329 – 3313Combined sources
Beta strandi334 – 3418Combined sources
Beta strandi345 – 3528Combined sources
Beta strandi363 – 3708Combined sources
Beta strandi378 – 38912Combined sources
Helixi391 – 3944Combined sources
Beta strandi399 – 41012Combined sources
Beta strandi423 – 43210Combined sources
Helixi436 – 4383Combined sources
Beta strandi446 – 4516Combined sources
Beta strandi455 – 46511Combined sources
Turni466 – 4694Combined sources
Beta strandi470 – 48011Combined sources
Beta strandi486 – 4938Combined sources
Beta strandi495 – 50713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TXKX-ray2.50A/B23-511[»]
ProteinModelPortaliP33136.
SMRiP33136. Positions 23-511.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33136.

Family & Domainsi

Domaini

Contains a large N-terminal domain that would serve as the catalytic core subunit, and a smaller C-terminal domain that would act to modulate this enzymatic activity.

Sequence similaritiesi

Belongs to the OpgD/OpgG family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DJ9. Bacteria.
COG3131. LUCA.
HOGENOMiHOG000288261.
InParanoidiP33136.
KOiK03670.
OMAiKQDEIMT.
OrthoDBiEOG6FZ487.
PhylomeDBiP33136.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.70.98.10. 1 hit.
HAMAPiMF_01069. MdoG_OpgG.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR007444. Glucan_biosyn_MdoG_C.
IPR014438. Glucan_biosyn_MdoGD.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR023704. MdoG_OpgG.
[Graphical view]
PfamiPF04349. MdoG. 1 hit.
[Graphical view]
PIRSFiPIRSF006281. MdoG. 1 hit.
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33136-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKMRWLSAA VMLTLYTSSS WAFSIDDVAK QAQSLAGKGY ETPKSNLPSV
60 70 80 90 100
FRDMKYADYQ QIQFNHDKAY WNNLKTPFKL EFYHQGMYFD TPVKINEVTA
110 120 130 140 150
TAVKRIKYSP DYFTFGDVQH DKDTVKDLGF AGFKVLYPIN SKDKNDEIVS
160 170 180 190 200
MLGASYFRVI GAGQVYGLSA RGLAIDTALP SGEEFPRFKE FWIERPKPTD
210 220 230 240 250
KRLTIYALLD SPRATGAYKF VVMPGRDTVV DVQSKIYLRD KVGKLGVAPL
260 270 280 290 300
TSMFLFGPNQ PSPANNYRPE LHDSNGLSIH AGNGEWIWRP LNNPKHLAVS
310 320 330 340 350
SFSMENPQGF GLLQRGRDFS RFEDLDDRYD LRPSAWVTPK GEWGKGSVEL
360 370 380 390 400
VEIPTNDETN DNIVAYWTPD QLPEPGKEMN FKYTITFSRD EDKLHAPDNA
410 420 430 440 450
WVQQTRRSTG DVKQSNLIRQ PDGTIAFVVD FTGAEMKKLP EDTPVTAQTS
460 470 480 490 500
IGDNGEIVES TVRYNPVTKG WRLVMRVKVK DAKKTTEMRA ALVNADQTLS
510
ETWSYQLPAN E
Length:511
Mass (Da):57,912
Last modified:October 1, 1993 - v1
Checksum:i0B53ECE270516C70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64197 Genomic DNA. Translation: CAA45521.1.
U00096 Genomic DNA. Translation: AAC74132.1.
AP009048 Genomic DNA. Translation: BAA35846.1.
PIRiS35417.
RefSeqiNP_415566.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74132; AAC74132; b1048.
BAA35846; BAA35846; BAA35846.
GeneIDi945005.
KEGGiecj:JW1035.
eco:b1048.
PATRICi32117335. VBIEscCol129921_1090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64197 Genomic DNA. Translation: CAA45521.1.
U00096 Genomic DNA. Translation: AAC74132.1.
AP009048 Genomic DNA. Translation: BAA35846.1.
PIRiS35417.
RefSeqiNP_415566.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TXKX-ray2.50A/B23-511[»]
ProteinModelPortaliP33136.
SMRiP33136. Positions 23-511.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260686. 329 interactions.
DIPiDIP-10177N.
IntActiP33136. 6 interactions.
STRINGi511145.b1048.

2D gel databases

SWISS-2DPAGEP33136.

Proteomic databases

EPDiP33136.
PaxDbiP33136.
PRIDEiP33136.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74132; AAC74132; b1048.
BAA35846; BAA35846; BAA35846.
GeneIDi945005.
KEGGiecj:JW1035.
eco:b1048.
PATRICi32117335. VBIEscCol129921_1090.

Organism-specific databases

EchoBASEiEB1831.
EcoGeneiEG11885. mdoG.

Phylogenomic databases

eggNOGiENOG4105DJ9. Bacteria.
COG3131. LUCA.
HOGENOMiHOG000288261.
InParanoidiP33136.
KOiK03670.
OMAiKQDEIMT.
OrthoDBiEOG6FZ487.
PhylomeDBiP33136.

Enzyme and pathway databases

UniPathwayiUPA00637.
BioCyciEcoCyc:EG11885-MONOMER.
ECOL316407:JW1035-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP33136.
PROiP33136.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.70.98.10. 1 hit.
HAMAPiMF_01069. MdoG_OpgG.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR007444. Glucan_biosyn_MdoG_C.
IPR014438. Glucan_biosyn_MdoGD.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR023704. MdoG_OpgG.
[Graphical view]
PfamiPF04349. MdoG. 1 hit.
[Graphical view]
PIRSFiPIRSF006281. MdoG. 1 hit.
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae."
    Loubens I., Debarbieux L., Bohin A., Lacroix J.-M., Bohin J.-P.
    Mol. Microbiol. 10:329-340(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-34.
    Strain: K12 / EMG2.
  6. "Structural analysis of Escherichia coli OpgG, a protein required for the biosynthesis of osmoregulated periplasmic glucans."
    Hanoulle X., Rollet E., Clantin B., Landrieu I., Oedberg-Ferragut C., Lippens G., Bohin J.-P., Villeret V.
    J. Mol. Biol. 342:195-205(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 23-511.

Entry informationi

Entry nameiOPGG_ECOLI
AccessioniPrimary (citable) accession number: P33136
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 16, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.