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P33129

- HTRE_ECOLI

UniProt

P33129 - HTRE_ECOLI

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Protein

Outer membrane usher protein HtrE

Gene

htrE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Part of the yadCKLM-htrE-yadVN fimbrial operon. Could contribute to adhesion to various surfaces in specific environmental niches. Probably involved in the export and assembly of fimbrial subunits across the outer membrane.1 Publication

GO - Molecular functioni

  1. transporter activity Source: InterPro

GO - Biological processi

  1. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fimbrium biogenesis, Stress response, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11972-MONOMER.
ECOL316407:JW0135-MONOMER.

Protein family/group databases

TCDBi1.B.11.3.3. the outer membrane fimbrial usher porin (fup) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane usher protein HtrE
Alternative name(s):
Heat shock protein E
Gene namesi
Name:htrE
Ordered Locus Names:b0139, JW0135
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11972. htrE.

Subcellular locationi

GO - Cellular componenti

  1. cell outer membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the operon under classical laboratory conditions does not result in any major effect on E.coli capacity to form biofilms compared with the wild-type strain.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29291 PublicationAdd
BLAST
Chaini30 – 865836Outer membrane usher protein HtrEPRO_0000009319Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi838 ↔ 862Sequence Analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP33129.
PRIDEiP33129.

Expressioni

Inductioni

Repressed by H-NS. Induced by heat shock.2 Publications

Gene expression databases

GenevestigatoriP33129.

Interactioni

Protein-protein interaction databases

DIPiDIP-9953N.
IntActiP33129. 9 interactions.
MINTiMINT-1300601.
STRINGi511145.b0139.

Structurei

3D structure databases

ProteinModelPortaliP33129.
SMRiP33129. Positions 61-850.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the fimbrial export usher family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiCOG3188.
HOGENOMiHOG000111416.
InParanoidiP33129.
KOiK07347.
OMAiAYTTGET.
OrthoDBiEOG6GJBP9.
PhylomeDBiP33129.

Family and domain databases

InterProiIPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view]
PANTHERiPTHR30451. PTHR30451. 1 hit.
PfamiPF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view]
PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33129-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTIEYTKNYH HLTRIATFCA LLYCNTAFSA ELVEYDHTFL MGQNASNIDL
60 70 80 90 100
SRYSEGNPAI PGVYDVSVYV NDQPIINQSI TFVAIEGKKN AQACITLKNL
110 120 130 140 150
LQFHINSPDI NNEKAVLLAR DETLGNCLNL TEIIPQASVR YDVNDQRLDI
160 170 180 190 200
DVPQAWVMKN YQNYVDPSLW ENGINAAMLS YNLNGYHSET PGRKNESIYA
210 220 230 240 250
AFNGGMNLGA WRLRASGNYN WMTDSGSNYD FKNRYVQRDI ASLRSQLILG
260 270 280 290 300
ESYTTGETFD SVSIRGIRLY SDSRMLPPTL ASFAPIIHGV ANTNAKVTIT
310 320 330 340 350
QGGYKIYETT VPPGAFVIDD LSPSGYGSDL IVTIEESDGS KRTFSQPFSS
360 370 380 390 400
VVQMLRPGVG RWDISGGQVL KDDIQDEPNL FQASYYYGLN NYLTGYTGIQ
410 420 430 440 450
ITDNNYTAGL LGLGLNTSVG AFSFDVTHSN VRIPDDKTYQ GQSYRVSWNK
460 470 480 490 500
LFEETSTSLN IAAYRYSTQN YLGLNDALTL IDEVKHPEQD LEPKSMRNYS
510 520 530 540 550
RMKNQVTVSI NQPLKFEKKD YGSFYLSGSW SDYWASGQNR SNYSIGYSNS
560 570 580 590 600
TSWGSYSVSA QRSWNEDGDT DDSVYLSFTI PIEKLLGTEQ RTSGFQSIDT
610 620 630 640 650
QISSDFKGNN QLNVSSSGYS DNARVSYSVN TGYTMNKASK DLSYVGGYAS
660 670 680 690 700
YESPWGTLAG SISANSDNSR QVSLSTDGGF VLHSGGLTFS NDSFSDSDTL
710 720 730 740 750
AVVQAPGAQG ARINYGNSTI DRWGYGVTSA LSPYHENRIA LDINDLENDV
760 770 780 790 800
ELKSTSAVAV PRQGSVVFAD FETVQGQSAI MNITRSDGKN IPFAADIYDE
810 820 830 840 850
QGNVIGNVGQ GGQAFVRGIE QQGNISIKWL EQSKPVSCLA HYQQSPEAEK
860
IAQSIILNGI RCQIQ
Length:865
Mass (Da):95,499
Last modified:November 1, 1997 - v3
Checksum:iF1748B551E4A5AAE
GO

Sequence cautioni

The sequence AAA23721.1 differs from that shown. Reason: Incorrect in position 61 onward due to a cloning artifact.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511S → T in AAA23721. (PubMed:8102362)Curated
Sequence conflicti810 – 8112QG → HR in AAA23721. (PubMed:8102362)Curated
Sequence conflicti849 – 8491E → P in AAA23721. (PubMed:8102362)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L00680 Genomic DNA. Translation: AAA23721.1. Sequence problems.
U00096 Genomic DNA. Translation: AAC73250.1.
AP009048 Genomic DNA. Translation: BAE76043.1.
PIRiC64737.
RefSeqiNP_414681.1. NC_000913.3.
YP_488442.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73250; AAC73250; b0139.
BAE76043; BAE76043; BAE76043.
GeneIDi12930744.
944819.
KEGGiecj:Y75_p0136.
eco:b0139.
PATRICi32115385. VBIEscCol129921_0144.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L00680 Genomic DNA. Translation: AAA23721.1 . Sequence problems.
U00096 Genomic DNA. Translation: AAC73250.1 .
AP009048 Genomic DNA. Translation: BAE76043.1 .
PIRi C64737.
RefSeqi NP_414681.1. NC_000913.3.
YP_488442.1. NC_007779.1.

3D structure databases

ProteinModelPortali P33129.
SMRi P33129. Positions 61-850.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9953N.
IntActi P33129. 9 interactions.
MINTi MINT-1300601.
STRINGi 511145.b0139.

Protein family/group databases

TCDBi 1.B.11.3.3. the outer membrane fimbrial usher porin (fup) family.

Proteomic databases

PaxDbi P33129.
PRIDEi P33129.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73250 ; AAC73250 ; b0139 .
BAE76043 ; BAE76043 ; BAE76043 .
GeneIDi 12930744.
944819.
KEGGi ecj:Y75_p0136.
eco:b0139.
PATRICi 32115385. VBIEscCol129921_0144.

Organism-specific databases

EchoBASEi EB1915.
EcoGenei EG11972. htrE.

Phylogenomic databases

eggNOGi COG3188.
HOGENOMi HOG000111416.
InParanoidi P33129.
KOi K07347.
OMAi AYTTGET.
OrthoDBi EOG6GJBP9.
PhylomeDBi P33129.

Enzyme and pathway databases

BioCyci EcoCyc:EG11972-MONOMER.
ECOL316407:JW0135-MONOMER.

Miscellaneous databases

PROi P33129.

Gene expression databases

Genevestigatori P33129.

Family and domain databases

InterProi IPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view ]
PANTHERi PTHR30451. PTHR30451. 1 hit.
Pfami PF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view ]
PROSITEi PS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and transcriptional analysis of the Escherichia coli htrE operon which is homologous to pap and related pilin operons."
    Raina S., Missiakas D., Baird L., Kumar S., Georgopoulos C.
    J. Bacteriol. 175:5009-5021(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: K12.
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Raina S.
    Submitted (DEC-1994) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 30-33.
  6. "Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities."
    Korea C.G., Badouraly R., Prevost M.C., Ghigo J.M., Beloin C.
    Environ. Microbiol. 12:1957-1977(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiHTRE_ECOLI
AccessioniPrimary (citable) accession number: P33129
Secondary accession number(s): Q2MCG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The operon is cryptic under classical laboratory conditions, but is functional when constitutively expressed.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3