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P33129

- HTRE_ECOLI

UniProt

P33129 - HTRE_ECOLI

Protein

Outer membrane usher protein HtrE

Gene

htrE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 3 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Part of the yadCKLM-htrE-yadVN fimbrial operon. Could contribute to adhesion to various surfaces in specific environmental niches. Probably involved in the export and assembly of fimbrial subunits across the outer membrane.1 Publication

    GO - Molecular functioni

    1. transporter activity Source: InterPro

    GO - Biological processi

    1. response to stress Source: UniProtKB-KW

    Keywords - Biological processi

    Fimbrium biogenesis, Stress response, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11972-MONOMER.
    ECOL316407:JW0135-MONOMER.

    Protein family/group databases

    TCDBi1.B.11.3.3. the outer membrane fimbrial usher porin (fup) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer membrane usher protein HtrE
    Alternative name(s):
    Heat shock protein E
    Gene namesi
    Name:htrE
    Ordered Locus Names:b0139, JW0135
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11972. htrE.

    Subcellular locationi

    GO - Cellular componenti

    1. cell outer membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell outer membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Deletion of the operon under classical laboratory conditions does not result in any major effect on E.coli capacity to form biofilms compared with the wild-type strain.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 29291 PublicationAdd
    BLAST
    Chaini30 – 865836Outer membrane usher protein HtrEPRO_0000009319Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi838 ↔ 862Sequence Analysis

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP33129.
    PRIDEiP33129.

    Expressioni

    Inductioni

    Repressed by H-NS. Induced by heat shock.2 Publications

    Gene expression databases

    GenevestigatoriP33129.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-9953N.
    IntActiP33129. 9 interactions.
    MINTiMINT-1300601.
    STRINGi511145.b0139.

    Structurei

    3D structure databases

    ProteinModelPortaliP33129.
    SMRiP33129. Positions 61-850.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the fimbrial export usher family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane beta strand

    Phylogenomic databases

    eggNOGiCOG3188.
    HOGENOMiHOG000111416.
    KOiK07347.
    OMAiAYTTGET.
    OrthoDBiEOG6GJBP9.
    PhylomeDBiP33129.

    Family and domain databases

    InterProiIPR000015. Fimb_usher.
    IPR018030. Fimbrial_membr_usher_CS.
    IPR025949. PapC-like_C.
    IPR025885. PapC_N_dom.
    [Graphical view]
    PfamiPF13953. PapC_C. 1 hit.
    PF13954. PapC_N. 1 hit.
    PF00577. Usher. 1 hit.
    [Graphical view]
    PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P33129-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTIEYTKNYH HLTRIATFCA LLYCNTAFSA ELVEYDHTFL MGQNASNIDL    50
    SRYSEGNPAI PGVYDVSVYV NDQPIINQSI TFVAIEGKKN AQACITLKNL 100
    LQFHINSPDI NNEKAVLLAR DETLGNCLNL TEIIPQASVR YDVNDQRLDI 150
    DVPQAWVMKN YQNYVDPSLW ENGINAAMLS YNLNGYHSET PGRKNESIYA 200
    AFNGGMNLGA WRLRASGNYN WMTDSGSNYD FKNRYVQRDI ASLRSQLILG 250
    ESYTTGETFD SVSIRGIRLY SDSRMLPPTL ASFAPIIHGV ANTNAKVTIT 300
    QGGYKIYETT VPPGAFVIDD LSPSGYGSDL IVTIEESDGS KRTFSQPFSS 350
    VVQMLRPGVG RWDISGGQVL KDDIQDEPNL FQASYYYGLN NYLTGYTGIQ 400
    ITDNNYTAGL LGLGLNTSVG AFSFDVTHSN VRIPDDKTYQ GQSYRVSWNK 450
    LFEETSTSLN IAAYRYSTQN YLGLNDALTL IDEVKHPEQD LEPKSMRNYS 500
    RMKNQVTVSI NQPLKFEKKD YGSFYLSGSW SDYWASGQNR SNYSIGYSNS 550
    TSWGSYSVSA QRSWNEDGDT DDSVYLSFTI PIEKLLGTEQ RTSGFQSIDT 600
    QISSDFKGNN QLNVSSSGYS DNARVSYSVN TGYTMNKASK DLSYVGGYAS 650
    YESPWGTLAG SISANSDNSR QVSLSTDGGF VLHSGGLTFS NDSFSDSDTL 700
    AVVQAPGAQG ARINYGNSTI DRWGYGVTSA LSPYHENRIA LDINDLENDV 750
    ELKSTSAVAV PRQGSVVFAD FETVQGQSAI MNITRSDGKN IPFAADIYDE 800
    QGNVIGNVGQ GGQAFVRGIE QQGNISIKWL EQSKPVSCLA HYQQSPEAEK 850
    IAQSIILNGI RCQIQ 865
    Length:865
    Mass (Da):95,499
    Last modified:November 1, 1997 - v3
    Checksum:iF1748B551E4A5AAE
    GO

    Sequence cautioni

    The sequence AAA23721.1 differs from that shown. Reason: Incorrect in position 61 onward due to a cloning artifact.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti51 – 511S → T in AAA23721. (PubMed:8102362)Curated
    Sequence conflicti810 – 8112QG → HR in AAA23721. (PubMed:8102362)Curated
    Sequence conflicti849 – 8491E → P in AAA23721. (PubMed:8102362)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00680 Genomic DNA. Translation: AAA23721.1. Sequence problems.
    U00096 Genomic DNA. Translation: AAC73250.1.
    AP009048 Genomic DNA. Translation: BAE76043.1.
    PIRiC64737.
    RefSeqiNP_414681.1. NC_000913.3.
    YP_488442.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73250; AAC73250; b0139.
    BAE76043; BAE76043; BAE76043.
    GeneIDi12930744.
    944819.
    KEGGiecj:Y75_p0136.
    eco:b0139.
    PATRICi32115385. VBIEscCol129921_0144.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00680 Genomic DNA. Translation: AAA23721.1 . Sequence problems.
    U00096 Genomic DNA. Translation: AAC73250.1 .
    AP009048 Genomic DNA. Translation: BAE76043.1 .
    PIRi C64737.
    RefSeqi NP_414681.1. NC_000913.3.
    YP_488442.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P33129.
    SMRi P33129. Positions 61-850.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9953N.
    IntActi P33129. 9 interactions.
    MINTi MINT-1300601.
    STRINGi 511145.b0139.

    Protein family/group databases

    TCDBi 1.B.11.3.3. the outer membrane fimbrial usher porin (fup) family.

    Proteomic databases

    PaxDbi P33129.
    PRIDEi P33129.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73250 ; AAC73250 ; b0139 .
    BAE76043 ; BAE76043 ; BAE76043 .
    GeneIDi 12930744.
    944819.
    KEGGi ecj:Y75_p0136.
    eco:b0139.
    PATRICi 32115385. VBIEscCol129921_0144.

    Organism-specific databases

    EchoBASEi EB1915.
    EcoGenei EG11972. htrE.

    Phylogenomic databases

    eggNOGi COG3188.
    HOGENOMi HOG000111416.
    KOi K07347.
    OMAi AYTTGET.
    OrthoDBi EOG6GJBP9.
    PhylomeDBi P33129.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11972-MONOMER.
    ECOL316407:JW0135-MONOMER.

    Miscellaneous databases

    PROi P33129.

    Gene expression databases

    Genevestigatori P33129.

    Family and domain databases

    InterProi IPR000015. Fimb_usher.
    IPR018030. Fimbrial_membr_usher_CS.
    IPR025949. PapC-like_C.
    IPR025885. PapC_N_dom.
    [Graphical view ]
    Pfami PF13953. PapC_C. 1 hit.
    PF13954. PapC_N. 1 hit.
    PF00577. Usher. 1 hit.
    [Graphical view ]
    PROSITEi PS01151. FIMBRIAL_USHER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and transcriptional analysis of the Escherichia coli htrE operon which is homologous to pap and related pilin operons."
      Raina S., Missiakas D., Baird L., Kumar S., Georgopoulos C.
      J. Bacteriol. 175:5009-5021(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
      Strain: K12.
    2. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
      Fujita N., Mori H., Yura T., Ishihama A.
      Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. Raina S.
      Submitted (DEC-1994) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 30-33.
    6. "Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities."
      Korea C.G., Badouraly R., Prevost M.C., Ghigo J.M., Beloin C.
      Environ. Microbiol. 12:1957-1977(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiHTRE_ECOLI
    AccessioniPrimary (citable) accession number: P33129
    Secondary accession number(s): Q2MCG3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 123 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The operon is cryptic under classical laboratory conditions, but is functional when constitutively expressed.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3