P33129 (HTRE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Outer membrane usher protein HtrE

Alternative name(s):
Heat shock protein E

Gene names

Name:	htrE
Ordered Locus Names:	b0139, JW0135

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	865 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable porin-like protein necessary for the assembly of a pilin-type protein.
Subcellular location	Cell outer membrane; Multi-pass membrane protein By similarity.
Induction	By heat shock.
Sequence similarities	Belongs to the fimbrial export usher family.
Sequence caution	The sequence AAA23721.1 differs from that shown. Reason: Incorrect in position 61 onward due to a cloning artifact.

Ontologies

Keywords
Biological process	Fimbrium biogenesis Stress response Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal Transmembrane Transmembrane beta strand
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Ref.5
	Chain	30 – 865	836	Outer membrane usher protein HtrE		PRO_0000009319
Amino acid modifications
	Disulfide bond	838 ↔ 862		Potential
Experimental info
	Sequence conflict	51	1	S → T in AAA23721. Ref.1
	Sequence conflict	810 – 811	2	QG → HR in AAA23721. Ref.1
	Sequence conflict	849	1	E → P in AAA23721. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33129 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: F1748B551E4A5AAE	FASTA	865	95,499
	10 20 30 40 50 60 MTIEYTKNYH HLTRIATFCA LLYCNTAFSA ELVEYDHTFL MGQNASNIDL SRYSEGNPAI 70 80 90 100 110 120 PGVYDVSVYV NDQPIINQSI TFVAIEGKKN AQACITLKNL LQFHINSPDI NNEKAVLLAR 130 140 150 160 170 180 DETLGNCLNL TEIIPQASVR YDVNDQRLDI DVPQAWVMKN YQNYVDPSLW ENGINAAMLS 190 200 210 220 230 240 YNLNGYHSET PGRKNESIYA AFNGGMNLGA WRLRASGNYN WMTDSGSNYD FKNRYVQRDI 250 260 270 280 290 300 ASLRSQLILG ESYTTGETFD SVSIRGIRLY SDSRMLPPTL ASFAPIIHGV ANTNAKVTIT 310 320 330 340 350 360 QGGYKIYETT VPPGAFVIDD LSPSGYGSDL IVTIEESDGS KRTFSQPFSS VVQMLRPGVG 370 380 390 400 410 420 RWDISGGQVL KDDIQDEPNL FQASYYYGLN NYLTGYTGIQ ITDNNYTAGL LGLGLNTSVG 430 440 450 460 470 480 AFSFDVTHSN VRIPDDKTYQ GQSYRVSWNK LFEETSTSLN IAAYRYSTQN YLGLNDALTL 490 500 510 520 530 540 IDEVKHPEQD LEPKSMRNYS RMKNQVTVSI NQPLKFEKKD YGSFYLSGSW SDYWASGQNR 550 560 570 580 590 600 SNYSIGYSNS TSWGSYSVSA QRSWNEDGDT DDSVYLSFTI PIEKLLGTEQ RTSGFQSIDT 610 620 630 640 650 660 QISSDFKGNN QLNVSSSGYS DNARVSYSVN TGYTMNKASK DLSYVGGYAS YESPWGTLAG 670 680 690 700 710 720 SISANSDNSR QVSLSTDGGF VLHSGGLTFS NDSFSDSDTL AVVQAPGAQG ARINYGNSTI 730 740 750 760 770 780 DRWGYGVTSA LSPYHENRIA LDINDLENDV ELKSTSAVAV PRQGSVVFAD FETVQGQSAI 790 800 810 820 830 840 MNITRSDGKN IPFAADIYDE QGNVIGNVGQ GGQAFVRGIE QQGNISIKWL EQSKPVSCLA 850 860 HYQQSPEAEK IAQSIILNGI RCQIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and transcriptional analysis of the Escherichia coli htrE operon which is homologous to pap and related pilin operons." Raina S., Missiakas D., Baird L., Kumar S., Georgopoulos C. J. Bacteriol. 175:5009-5021(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	Raina S. Submitted (DEC-1994) to UniProtKB Cited for: PROTEIN SEQUENCE OF 30-33.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L00680 Genomic DNA. Translation: AAA23721.1. Sequence problems. U00096 Genomic DNA. Translation: AAC73250.1. AP009048 Genomic DNA. Translation: BAE76043.1.
PIR	C64737.
RefSeq	NP_414681.1. NC_000913.2. YP_488442.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P33129.
SMR	P33129. Positions 35-850.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9953N.
IntAct	P33129. 5 interactions.
MINT	MINT-1300601.
STRING	511145.b0139.
Protein family/group databases
TCDB	1.B.11.3.3. outer membrane fimbrial usher porin (FUP) family.
Proteomic databases
PaxDb	P33129.
PRIDE	P33129.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73250; AAC73250; b0139. BAE76043; BAE76043; BAE76043.
GeneID	12930744. 944819.
KEGG	ecj:Y75_p0136. eco:b0139.
PATRIC	32115385. VBIEscCol129921_0144.
Organism-specific databases
EchoBASE	EB1915.
EcoGene	EG11972. htrE.
Phylogenomic databases
eggNOG	COG3188.
HOGENOM	HOG000111416.
KO	K07347.
OMA	AYTTGET.
ProtClustDB	PRK09828.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11972-MONOMER. ECOL316407:JW0135-MONOMER.
Gene expression databases
Genevestigator	P33129.
Family and domain databases
InterPro	IPR000015. Fimb_usher. IPR018030. Fimbrial_membr_usher_CS. IPR025949. PapC-like_C. IPR025885. PapC_N_dom. [Graphical view]
Pfam	PF13953. PapC_C. 1 hit. PF13954. PapC_N. 1 hit. PF00577. Usher. 1 hit. [Graphical view]
PROSITE	PS01151. FIMBRIAL_USHER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HTRE_ECOLI

Accession

Primary (citable) accession number: P33129
Secondary accession number(s): Q2MCG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program