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P33124

- ACSL6_RAT

UniProt

P33124 - ACSL6_RAT

Protein

Long-chain-fatty-acid--CoA ligase 6

Gene

Acsl6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid.

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: RGD
    3. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to insulin stimulus Source: RGD
    2. fatty acid transport Source: RGD
    3. long-chain fatty acid metabolic process Source: RGD
    4. neuroblast proliferation Source: Ensembl
    5. neuron development Source: RGD
    6. phospholipid biosynthetic process Source: RGD
    7. positive regulation of neuron projection development Source: RGD
    8. positive regulation of plasma membrane long-chain fatty acid transport Source: RGD
    9. positive regulation of triglyceride biosynthetic process Source: RGD
    10. response to gravity Source: RGD
    11. response to hypoxia Source: RGD
    12. response to nutrient Source: RGD
    13. response to steroid hormone Source: RGD
    14. triglyceride metabolic process Source: RGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi6.2.1.3. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long-chain-fatty-acid--CoA ligase 6 (EC:6.2.1.3)
    Alternative name(s):
    Long-chain acyl-CoA synthetase 6
    Short name:
    LACS 6
    Long-chain-fatty-acid--CoA ligase, brain isozyme
    Gene namesi
    Name:Acsl6
    Synonyms:Acs2, Facl6
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi69403. Acsl6.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. mitochondrial outer membrane Source: UniProtKB-SubCell
    4. nucleus Source: RGD
    5. peroxisomal membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 697697Long-chain-fatty-acid--CoA ligase 6PRO_0000193117Add
    BLAST

    Proteomic databases

    PaxDbiP33124.
    PRIDEiP33124.

    PTM databases

    PhosphoSiteiP33124.

    Expressioni

    Tissue specificityi

    Expressed predominantly in brain and, to a much lesser extent, in heart and adrenal.

    Developmental stagei

    Detected 5 days after birth, and increased in a coordinate fashion during the development of brain, and the amount did not decrease when myelination was completed.

    Gene expression databases

    ArrayExpressiP33124.
    GenevestigatoriP33124.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4564901.

    Structurei

    3D structure databases

    ProteinModelPortaliP33124.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini46 – 697652CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4521Helical; Signal-anchor for type III membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1022.
    GeneTreeiENSGT00690000101725.
    HOGENOMiHOG000159459.
    HOVERGENiHBG050452.
    KOiK01897.
    OMAiMFERMVQ.
    OrthoDBiEOG71CFKN.
    PhylomeDBiP33124.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P33124-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQTQEILRIL RLPELSDLGQ FFRSLSATTL VSMGALAAIL AYWLTHRPKA    50
    LQPPCNLLMQ SEEVEDSGGA RRSVIGDCTQ LLTHYYDDAR TMYQVFRRGL 100
    SISGNGPCLG FRKPEQPYQW LSYQEVAKRA EFLGSGLLQH DCKVGTEQFI 150
    GVFAQNRPEW IIAELACYTY SMVVVPLYDT LGPGSIRYII NTADICTVIV 200
    DKPHKAILLL EHVERKETPG LKLVILMEPF DDALRERGKK CGVDIKSMQA 250
    IEDSGQENHR VPVPPRPDDL SIVCFTSGTT GNPKGAMLTH GNVVADFSGF 300
    LKVTESQWAP TCADVHFSYL PLAHMFERMV QSVVYCHGGR VGFFQGDIRL 350
    LSDDMKALRP TIFPVVPRLL NRMYDKIFHQ ADTSLKRWLL EFAAKRKQAE 400
    VRSGIIRNNS IWDELFFNKI QASLGGHVRM IVTGAAPASP TVLGFLRAAL 450
    GCQVYEGYGQ TECTAGCTFT TPGDWTSGHV GAPLPCNHIK LVDAEELNYW 500
    TSKGEGEICV KGPNVFKGYL KDEDRTKEAL DSDGWLHTGD IGKWLPEGTL 550
    KIIDRKKHIF KLAQGEYVAP EKIENIYIRS EPVAQIYVHG DSLKAFLVGI 600
    VVPDPEVMPC WAQKKGIEGN YQELCKSKEL KKAILDDMVM LGKESGLHSF 650
    EQVKAIYIHC DMFSVQNGLL TPTLKAKRPE LREYFKKQIE ELYSISM 697
    Length:697
    Mass (Da):78,180
    Last modified:October 1, 1993 - v1
    Checksum:i0B0CA7EFD653E2BE
    GO
    Isoform 2 (identifier: P33124-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MLTFFLVSGGSLWLFAEIALSLLEKM

    Show »
    Length:722
    Mass (Da):80,949
    Checksum:i3E3F54AAFDF059F8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti558 – 5581H → D in AAB19809. (PubMed:1654331)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MLTFFLVSGGSLWLFAEIAL SLLEKM in isoform 2. 1 PublicationVSP_037825

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S56508 mRNA. Translation: AAB19809.2.
    D10041 mRNA. Translation: BAA00932.1.
    CB582512 mRNA. No translation available.
    PIRiJX0205.
    RefSeqiNP_570095.1. NM_130739.1.
    UniGeneiRn.33697.

    Genome annotation databases

    EnsembliENSRNOT00000030760; ENSRNOP00000033248; ENSRNOG00000026745. [P33124-1]
    ENSRNOT00000058817; ENSRNOP00000055608; ENSRNOG00000026745. [P33124-2]
    GeneIDi117243.
    KEGGirno:117243.
    UCSCiRGD:69403. rat. [P33124-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S56508 mRNA. Translation: AAB19809.2 .
    D10041 mRNA. Translation: BAA00932.1 .
    CB582512 mRNA. No translation available.
    PIRi JX0205.
    RefSeqi NP_570095.1. NM_130739.1.
    UniGenei Rn.33697.

    3D structure databases

    ProteinModelPortali P33124.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4564901.

    PTM databases

    PhosphoSitei P33124.

    Proteomic databases

    PaxDbi P33124.
    PRIDEi P33124.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000030760 ; ENSRNOP00000033248 ; ENSRNOG00000026745 . [P33124-1 ]
    ENSRNOT00000058817 ; ENSRNOP00000055608 ; ENSRNOG00000026745 . [P33124-2 ]
    GeneIDi 117243.
    KEGGi rno:117243.
    UCSCi RGD:69403. rat. [P33124-1 ]

    Organism-specific databases

    CTDi 23305.
    RGDi 69403. Acsl6.

    Phylogenomic databases

    eggNOGi COG1022.
    GeneTreei ENSGT00690000101725.
    HOGENOMi HOG000159459.
    HOVERGENi HBG050452.
    KOi K01897.
    OMAi MFERMVQ.
    OrthoDBi EOG71CFKN.
    PhylomeDBi P33124.

    Enzyme and pathway databases

    BRENDAi 6.2.1.3. 5301.

    Miscellaneous databases

    NextBioi 620076.

    Gene expression databases

    ArrayExpressi P33124.
    Genevestigatori P33124.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and sequencing of cDNA encoding the phosphatidylinositol kinase from rat brain."
      Yamakawa A., Nishizawa M., Fujiwara K.T., Kawai S., Kawasaki H., Suzuki K., Takenawa T.
      J. Biol. Chem. 266:17580-17583(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 491-503; 544-551; 573-579 AND 633-636.
      Tissue: Brain.
    2. Cited for: RETRACTION.
    3. "Cloning and functional expression of a novel long-chain acyl-CoA synthetase expressed in brain."
      Fujino T., Yamamoto T.
      J. Biochem. 111:197-203(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: Wistar.
      Tissue: Brain.
    4. "Amgen rat EST program."
      Amgen EST program
      Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-160 (ISOFORM 2).
      Tissue: Spinal ganglion.

    Entry informationi

    Entry nameiACSL6_RAT
    AccessioniPrimary (citable) accession number: P33124
    Secondary accession number(s): Q63835
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3