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Protein

Aspartate ammonia-lyase

Gene

aspA

Organism
Serratia marcescens
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-aspartate = fumarate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate ammonia-lyase (EC:4.3.1.1)
Short name:
Aspartase
Gene namesi
Name:aspA
OrganismiSerratia marcescens
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Aspartate ammonia-lyasePRO_0000161345Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP33109.
SMRiP33109. Positions 1-459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni143 – 1453Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR004708. ApsA.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00839. aspA. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33109-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNNIRIEED LLGTREVPAD AYYGVHTLRA IENFYISNSK ISDVPEFVRG
60 70 80 90 100
MVMVKKAAAM ANKELKTIPR KIADVIIQAC DEVLDKGKCM DQFPVDVFQG
110 120 130 140 150
GAGTSLNMNT NEVLANIGLE LMGHQKGEYQ YLNPNDHLNK CQSTNDAYPT
160 170 180 190 200
GFRIAVYASN QKLIDAINQL REGFDRKAKE FETILKMGRT QLQDAVPMTL
210 220 230 240 250
GQEFHAFSVL LNEETRNLHR TATLLLEVNL GATAIGTALN TPEGYQPLAV
260 270 280 290 300
QKLAEVSGLP VVPAEDLIEA TSDCGAYVMV HSALKRLAVK LSKICNDLRL
310 320 330 340 350
LSSGPRAGLN EINLPELQAG SSIMPAKVNP VVPEVVNQVC FKVIGNDTCV
360 370 380 390 400
TMAAEAGQLQ LNVMEPVIGQ AMFESIHILT NACYNLLEKC INGITANKEV
410 420 430 440 450
CEHYVFNSIG IVTYLNPFIG HHNGDIVGKI CAETGKSVRE VVLERGLLTE
460 470
AELDDIFSVE NLMHPAYKAK RYTDENEQ
Length:478
Mass (Da):52,543
Last modified:October 1, 1993 - v1
Checksum:i254A7B455838D2A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13252 Genomic DNA. Translation: BAA02518.1.
PIRiJS0764.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13252 Genomic DNA. Translation: BAA02518.1.
PIRiJS0764.

3D structure databases

ProteinModelPortaliP33109.
SMRiP33109. Positions 1-459.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR004708. ApsA.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00839. aspA. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Construction of a versatile promoter analysis vector and its use for analysis of the Serratia marcescens aspartase promoter region."
    Omori K., Akatsuka H., Komatsubara S.
    Plasmid 32:233-237(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sr41.

Entry informationi

Entry nameiASPA_SERMA
AccessioniPrimary (citable) accession number: P33109
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 14, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.