P33109 (ASPA_SERMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate ammonia-lyase
Short name=Aspartase
EC=4.3.1.1

Gene names

Name:

aspA

Organism

Serratia marcescens

Taxonomic identifier

615 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate = fumarate + NH3.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Aspartase subfamily.

Ontologies

Keywords
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	aspartate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	aspartate ammonia-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Aspartate ammonia-lyase		PRO_0000161345
Regions
	Region	143 – 145	3	Substrate binding By similarity
Sites
	Binding site	104	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P33109 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 254A7B455838D2A5	FASTA	478	52,543
	10 20 30 40 50 60 MSNNIRIEED LLGTREVPAD AYYGVHTLRA IENFYISNSK ISDVPEFVRG MVMVKKAAAM 70 80 90 100 110 120 ANKELKTIPR KIADVIIQAC DEVLDKGKCM DQFPVDVFQG GAGTSLNMNT NEVLANIGLE 130 140 150 160 170 180 LMGHQKGEYQ YLNPNDHLNK CQSTNDAYPT GFRIAVYASN QKLIDAINQL REGFDRKAKE 190 200 210 220 230 240 FETILKMGRT QLQDAVPMTL GQEFHAFSVL LNEETRNLHR TATLLLEVNL GATAIGTALN 250 260 270 280 290 300 TPEGYQPLAV QKLAEVSGLP VVPAEDLIEA TSDCGAYVMV HSALKRLAVK LSKICNDLRL 310 320 330 340 350 360 LSSGPRAGLN EINLPELQAG SSIMPAKVNP VVPEVVNQVC FKVIGNDTCV TMAAEAGQLQ 370 380 390 400 410 420 LNVMEPVIGQ AMFESIHILT NACYNLLEKC INGITANKEV CEHYVFNSIG IVTYLNPFIG 430 440 450 460 470 HHNGDIVGKI CAETGKSVRE VVLERGLLTE AELDDIFSVE NLMHPAYKAK RYTDENEQ

References

[1]	"Construction of a versatile promoter analysis vector and its use for analysis of the Serratia marcescens aspartase promoter region." Omori K., Akatsuka H., Komatsubara S. Plasmid 32:233-237(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sr41.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13252 Genomic DNA. Translation: BAA02518.1.
PIR	JS0764.
3D structure databases
ProteinModelPortal	P33109.
SMR	P33109. Positions 1-459.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.275.10. 1 hit.
InterPro	IPR004708. ApsA. IPR003031. D_crystallin. IPR024083. Fumarase/histidase_N. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR022761. Fumarate_lyase_N. IPR008948. L-Aspartase-like. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00839. aspA. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPA_SERMA

Accession

Primary (citable) accession number: P33109

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program