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Protein

Aspartate aminotransferase, cytoplasmic

Gene

GOT1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity).By similarity

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
L-cysteine + 2-oxoglutarate = mercaptopyruvate + L-glutamate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39Aspartate; via amide nitrogenBy similarity1
Binding sitei141AspartateBy similarity1
Binding sitei195AspartateBy similarity1
Binding sitei387AspartateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-BTA-70263. Gluconeogenesis.
R-BTA-70614. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate aminotransferase, cytoplasmic (EC:2.6.1.1, EC:2.6.1.3)
Short name:
cAspAT
Alternative name(s):
Cysteine aminotransferase, cytoplasmic
Cysteine transaminase, cytoplasmic
Short name:
cCAT
Glutamate oxaloacetate transaminase 1
Transaminase A
Gene namesi
Name:GOT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 26

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Protein family/group databases

Allergomei11908. Bos d AATr.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001238772 – 413Aspartate aminotransferase, cytoplasmicAdd BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei259N6-(pyridoxal phosphate)lysine1

Proteomic databases

PaxDbiP33097.
PeptideAtlasiP33097.
PRIDEiP33097.

Expressioni

Gene expression databases

BgeeiENSBTAG00000011960.

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015873.

Structurei

3D structure databases

ProteinModelPortaliP33097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1411. Eukaryota.
COG1448. LUCA.
GeneTreeiENSGT00390000014081.
HOGENOMiHOG000185898.
HOVERGENiHBG000951.
InParanoidiP33097.
KOiK14454.
OMAiPNHKGVF.
OrthoDBiEOG091G06G3.
TreeFamiTF314089.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11879. PTHR11879. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PRINTSiPR00799. TRANSAMINASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPSIFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDSQPWV
60 70 80 90 100
LPVVRKVEQR IANDSSINHE YLPILGLAEF RTCASRLALG DDSPALQEKR
110 120 130 140 150
VGGVQCLGGT GALRIGAEFL ARWYNGTNNK DTPVYVSSPT WENHNGVFIA
160 170 180 190 200
AGFKDIRSYH YWDAAKRGLD LQGFLNDLEK APEFSIFVLH ACAHNPTGTD
210 220 230 240 250
PTPEQWKQIA SVMKRRFLFP FFDSAYQGFA SGSLEKDAWA IRYFVSEGFE
260 270 280 290 300
LFCAQSFSKN FGLYNERVGN LTVVAKEPDS ILRVLSQMEK IVRITWSNPP
310 320 330 340 350
AQGARIVART LSDPELFNEW TGNVKTMADR ILTMRSELRA RLEALKTPGT
360 370 380 390 400
WNHITEQIGM FSFTGLNPKQ VEYLINEKHI YLLPSGRINM CGLTTKNLEY
410
VATSIHEAVT KIQ
Length:413
Mass (Da):46,399
Last modified:January 23, 2007 - v3
Checksum:iEB43960F08081DD4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti228G → A in CAA46818 (PubMed:8224363).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66020 mRNA. Translation: CAA46818.1.
BT020856 mRNA. Translation: AAX08873.1.
BC105372 mRNA. Translation: AAI05373.1.
PIRiI46006. S21560.
RefSeqiNP_803468.1. NM_177502.2.
UniGeneiBt.17296.

Genome annotation databases

EnsembliENSBTAT00000015873; ENSBTAP00000015873; ENSBTAG00000011960.
GeneIDi281206.
KEGGibta:281206.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66020 mRNA. Translation: CAA46818.1.
BT020856 mRNA. Translation: AAX08873.1.
BC105372 mRNA. Translation: AAI05373.1.
PIRiI46006. S21560.
RefSeqiNP_803468.1. NM_177502.2.
UniGeneiBt.17296.

3D structure databases

ProteinModelPortaliP33097.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015873.

Protein family/group databases

Allergomei11908. Bos d AATr.

Proteomic databases

PaxDbiP33097.
PeptideAtlasiP33097.
PRIDEiP33097.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000015873; ENSBTAP00000015873; ENSBTAG00000011960.
GeneIDi281206.
KEGGibta:281206.

Organism-specific databases

CTDi2805.

Phylogenomic databases

eggNOGiKOG1411. Eukaryota.
COG1448. LUCA.
GeneTreeiENSGT00390000014081.
HOGENOMiHOG000185898.
HOVERGENiHBG000951.
InParanoidiP33097.
KOiK14454.
OMAiPNHKGVF.
OrthoDBiEOG091G06G3.
TreeFamiTF314089.

Enzyme and pathway databases

ReactomeiR-BTA-70263. Gluconeogenesis.
R-BTA-70614. Amino acid synthesis and interconversion (transamination).

Gene expression databases

BgeeiENSBTAG00000011960.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11879. PTHR11879. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PRINTSiPR00799. TRANSAMINASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAATC_BOVIN
AccessioniPrimary (citable) accession number: P33097
Secondary accession number(s): Q2KJF2, Q5E9R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.