Reviewed, UniProtKB/Swiss-Prot P33097 (AATC_BOVIN)

Names and origin

Protein names

Recommended name:
    Aspartate aminotransferase, cytoplasmic
    EC=2.6.1.1
Alternative name(s):
    Transaminase A
    Glutamate oxaloacetate transaminase 1

Gene names

Name:

GOT1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular function	L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 413	412	Aspartate aminotransferase, cytoplasmic		PRO_0000123877
Amino acid modifications
	Modified residue	66	1	Phosphoserine By similarity
	Modified residue	71	1	Phosphotyrosine By similarity
	Modified residue	259	1	N6-(pyridoxal phosphate)lysine
Experimental info
	Sequence conflict	228	1	G → A in CAA46818. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P33097-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: EB43960F08081DD4	FASTA	413	46,399
	10 20 30 40 50 60 MAPPSIFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDSQPWV LPVVRKVEQR 70 80 90 100 110 120 IANDSSINHE YLPILGLAEF RTCASRLALG DDSPALQEKR VGGVQCLGGT GALRIGAEFL 130 140 150 160 170 180 ARWYNGTNNK DTPVYVSSPT WENHNGVFIA AGFKDIRSYH YWDAAKRGLD LQGFLNDLEK 190 200 210 220 230 240 APEFSIFVLH ACAHNPTGTD PTPEQWKQIA SVMKRRFLFP FFDSAYQGFA SGSLEKDAWA 250 260 270 280 290 300 IRYFVSEGFE LFCAQSFSKN FGLYNERVGN LTVVAKEPDS ILRVLSQMEK IVRITWSNPP 310 320 330 340 350 360 AQGARIVART LSDPELFNEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITEQIGM 370 380 390 400 410 FSFTGLNPKQ VEYLINEKHI YLLPSGRINM CGLTTKNLEY VATSIHEAVT KIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of A cDNA encoding bovine cytosolic aspartate aminotransferase." Aurilia V., Palmisano A., Ferrara L., Cubellis M.V., Sannia G., Marino G. Int. J. Biochem. 25:1505-1509(1993) [PubMed: 8224363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X66020 mRNA. Translation: CAA46818.1. BT020856 mRNA. Translation: AAX08873.1. BC105372 mRNA. Translation: AAI05373.1.
IPI	IPI00694612.
PIR	S21560. I46006.
RefSeq	NP_803468.1.
UniGene	Bt.17296
3D structure databases
HSSP	HSSP built from PDB template 1AJS based on UniProtKB P00503.
SMR	P33097. Positions 2-413.
ModBase	Search...
Protein-protein interaction databases
STRING	P33097.
Genome annotation databases
Ensembl	ENSBTAT00000015873; ENSBTAP00000015873; ENSBTAG00000011960; Bos taurus. [Genome view]
GeneID	281206.
KEGG	bta:281206.
Organism-specific databases
CTD	281206.
Phylogenomic databases
HOVERGEN	P33097.
Enzyme and pathway databases
BRENDA	2.6.1.1. 251.
Family and domain databases
InterPro	IPR004839. Aminotransferase_I/II. IPR000796. Asp_trans. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11879. Asp_trans. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00799. TRANSAMINASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AATC_BOVIN

Accession

Primary (citable) accession number: P33097
Secondary accession number(s): Q2KJF2, Q5E9R4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	January 23, 2007
Last modified:	September 22, 2009
This is version 72 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)