P33085 (SHAKB_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Innexin shaking-B

Alternative name(s):
Protein passover

Gene names

Name:	shakB
Synonyms:	Pas, shak-B
ORF Names:	CG34358

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Structural component of the gap junctions at electrical synapses in distal and mid-depth levels in the lamina. Isoform Lethal forms voltage sensitive intercellular channels through homotypic interactions. Ref.8 Ref.9
Subunit structure	Monomer (isoform Lethal). Ref.9
Subcellular location	Cell membrane; Multi-pass membrane protein Potential. Cell junction › gap junction.
Tissue specificity	Isoform Neural is expressed in synapses of giant fibers (GF), in a large thoracic cell in location of postsynaptic target and optic lobe lamina and medulla. Isoform Lethal is expressed in embryonic mesodermal derivatives. During metamorphosis, both isoforms are dynamically expressed in pupal nervous system.
Disruption phenotype	Flies fail to jump in response to a light-off stimulus. Neural-specific mutants exhibit modified giant fiber system and gustatory response. Ref.2
Sequence similarities	Belongs to the pannexin family.

Ontologies

Keywords
Biological process	Behavior Ion transport Transport
Cellular component	Cell junction Cell membrane Gap junction Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell communication by electrical coupling Inferred from mutant phenotype PubMed 19084406. Source: FlyBase gap junction assembly Inferred from mutant phenotype Ref.9. Source: UniProtKB ion transport Inferred from electronic annotation. Source: UniProtKB-KW jump response Inferred from mutant phenotype PubMed 2127576. Source: FlyBase phototransduction Inferred from mutant phenotype PubMed 12154069. Source: FlyBase regulation of membrane depolarization Inferred from mutant phenotype PubMed 19084406. Source: FlyBase
Cellular_component	gap junction Inferred from direct assay Ref.9. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 19084406. Source: FlyBase
Molecular_function	gap junction channel activity Inferred from mutant phenotype Ref.9. Source: UniProtKB photoreceptor activity Inferred from mutant phenotype Ref.8. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform Lethal (identifier: P33085-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Neural (identifier: P33085-2)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     2-58: LDIFRGLKNL...GNPIDCVHTK → VSHVKIDSPV...GNPIDCVHTR
     72-116: QSTYTLKSLF...YKYYQWVCFC → HSTYTVVDAF...TKYYQWVAFT

Isoform B (identifier: P33085-3)

The sequence of this isoform differs from the canonical sequence as follows:
     121-121: A → P
     123-372: Missing.

Isoform D (identifier: P33085-4)

The sequence of this isoform differs from the canonical sequence as follows:
     2-116: LDIFRGLKNL...YKYYQWVCFC → YKPDTLTRRG...TKYYQWVAFT

Note: No experimental confirmation available.

Isoform E (identifier: P33085-6)

The sequence of this isoform differs from the canonical sequence as follows:
     2-119: LDIFRGLKNL...YQWVCFCLFF → MGRHCHVPKQ...QKKIPTTYNE

Note: No experimental confirmation available.

Isoform F (identifier: P33085-5)

The sequence of this isoform differs from the canonical sequence as follows:
     2-36: LDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILM → KKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFT
     37-116: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Innexin shaking-B		PRO_0000208500
Regions
	Topological domain	1 – 21	21	Cytoplasmic Potential
	Transmembrane	22 – 42	21	Helical; Potential
	Topological domain	43 – 110	68	Extracellular Potential
	Transmembrane	111 – 131	21	Helical; Potential
	Topological domain	132 – 182	51	Cytoplasmic Potential
	Transmembrane	183 – 203	21	Helical; Potential
	Topological domain	204 – 267	64	Extracellular Potential
	Transmembrane	268 – 288	21	Helical; Potential
	Topological domain	289 – 372	84	Cytoplasmic Potential
Natural variations
	Alternative sequence	2 – 119	118	LDIFR…FCLFF → MGRHCHVPKQTHHIQFAQLA QSPAVCGLSESAANLRYKLL LICHRVGIKIPRQKKIPTTY NE in isoform E.		VSP_037474
	Alternative sequence	2 – 116	115	LDIFR…WVCFC → YKPDTLTRRGSLRSLRSAPL LSTVLESTLSLTRIHPIASL ELPGLDYAVHSQSAFGAYGL AHPRDLATCTSLRSGLAAIT AASASAAGGVSQSQSALLGR YGPNASIRHGERKIVQPKRV LSRKLKPHLVADTVKQYISR AQRTTKKGSQEQQNMEFLRG VYAFMQVSRSSVSHVKIDSP VFRLHTNATVILLITFSIAV TTRQYVGNPIDCVHTRDIPE DVLNTYCWIHSTYTVVDAFM KKQGSEVPFPGVHNSQGRGP LTIKHTKYYQWVAFT in isoform D.		VSP_036913
	Alternative sequence	2 – 58	57	LDIFR…CVHTK → VSHVKIDSPVFRLHTNATVI LLITFSIAVTTRQYVGNPID CVHTR in isoform Neural.		VSP_002678
	Alternative sequence	2 – 36	35	LDIFR…VMILM → KKQGSEVPFPGVHNSQGRGP LTIKHTKYYQWVAFT in isoform F.		VSP_036914
	Alternative sequence	37 – 116	80	Missing in isoform F.		VSP_036915
	Alternative sequence	72 – 116	45	QSTYT…WVCFC → HSTYTVVDAFMKKQGSEVPF PGVHNSQGRGPLTIKHTKYY QWVAFT in isoform Neural.		VSP_002679
	Alternative sequence	121	1	A → P in isoform B.		VSP_012897
	Alternative sequence	123 – 372	250	Missing in isoform B.		VSP_012898
Experimental info
	Sequence conflict	232	1	I → M in AAA28745. Ref.2
	Sequence conflict	232	1	I → M in ABE73233. Ref.7
	Sequence conflict	247 – 249	3	SGE → GEV in AAC46584. Ref.4
Isoform D:
	Sequence conflict	3	1	K → E in ABE73233. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform Lethal (A) [UniParc]. Last modified December 1, 2000. Version 3. Checksum: 3DD1049D8F1D4481	FASTA	372	44,355
	10 20 30 40 50 60 MLDIFRGLKN LVKVSHVKTD SIVFRLHYSI TVMILMSFSL IITTRQYVGN PIDCVHTKDI 70 80 90 100 110 120 PEDVLNTYCW IQSTYTLKSL FLKKQGVSVP YPGIGNSDGD PADKKHYKYY QWVCFCLFFQ 130 140 150 160 170 180 AILFYTPRWL WKSWEGGKIH ALIMDLDIGI CSEAEKKQKK KLLLDYLWEN LRYHNWWAYR 190 200 210 220 230 240 YYVCELLALI NVIGQMFLMN RFFDGEFITF GLKVIDYMET DQEDRMDPMI YIFPRMTKCT 250 260 270 280 290 300 FFKYGSSGEV EKHDAICILP LNVVNEKIYI FLWFWFILLT FLTLLTLIYR VVIIFSPRMR 310 320 330 340 350 360 VYLFRMRFRL VRRDAIEIIV RRSKMGDWFL LYLLGENIDT VIFRDVVQDL ANRLGHNQHH 370 RVPGLKGEIQ DA
	Isoform Neural (C) [UniParc]. Checksum: B914B25C8D6E8DD5	FASTA	361	42,904
	10 20 30 40 50 60 MVSHVKIDSP VFRLHTNATV ILLITFSIAV TTRQYVGNPI DCVHTRDIPE DVLNTYCWIH 70 80 90 100 110 120 STYTVVDAFM KKQGSEVPFP GVHNSQGRGP LTIKHTKYYQ WVAFTLFFQA ILFYTPRWLW 130 140 150 160 170 180 KSWEGGKIHA LIMDLDIGIC SEAEKKQKKK LLLDYLWENL RYHNWWAYRY YVCELLALIN 190 200 210 220 230 240 VIGQMFLMNR FFDGEFITFG LKVIDYMETD QEDRMDPMIY IFPRMTKCTF FKYGSSGEVE 250 260 270 280 290 300 KHDAICILPL NVVNEKIYIF LWFWFILLTF LTLLTLIYRV VIIFSPRMRV YLFRMRFRLV 310 320 330 340 350 360 RRDAIEIIVR RSKMGDWFLL YLLGENIDTV IFRDVVQDLA NRLGHNQHHR VPGLKGEIQD A
	Isoform B [UniParc]. Checksum: D71A9AC29FB0A361	FASTA	122	14,147
	10 20 30 40 50 60 MLDIFRGLKN LVKVSHVKTD SIVFRLHYSI TVMILMSFSL IITTRQYVGN PIDCVHTKDI 70 80 90 100 110 120 PEDVLNTYCW IQSTYTLKSL FLKKQGVSVP YPGIGNSDGD PADKKHYKYY QWVCFCLFFQ PI
	Isoform D [UniParc]. Checksum: 3C517141DBCE54EA	FASTA	532	61,391
	10 20 30 40 50 60 MYKPDTLTRR GSLRSLRSAP LLSTVLESTL SLTRIHPIAS LELPGLDYAV HSQSAFGAYG 70 80 90 100 110 120 LAHPRDLATC TSLRSGLAAI TAASASAAGG VSQSQSALLG RYGPNASIRH GERKIVQPKR 130 140 150 160 170 180 VLSRKLKPHL VADTVKQYIS RAQRTTKKGS QEQQNMEFLR GVYAFMQVSR SSVSHVKIDS 190 200 210 220 230 240 PVFRLHTNAT VILLITFSIA VTTRQYVGNP IDCVHTRDIP EDVLNTYCWI HSTYTVVDAF 250 260 270 280 290 300 MKKQGSEVPF PGVHNSQGRG PLTIKHTKYY QWVAFTLFFQ AILFYTPRWL WKSWEGGKIH 310 320 330 340 350 360 ALIMDLDIGI CSEAEKKQKK KLLLDYLWEN LRYHNWWAYR YYVCELLALI NVIGQMFLMN 370 380 390 400 410 420 RFFDGEFITF GLKVIDYMET DQEDRMDPMI YIFPRMTKCT FFKYGSSGEV EKHDAICILP 430 440 450 460 470 480 LNVVNEKIYI FLWFWFILLT FLTLLTLIYR VVIIFSPRMR VYLFRMRFRL VRRDAIEIIV 490 500 510 520 530 RRSKMGDWFL LYLLGENIDT VIFRDVVQDL ANRLGHNQHH RVPGLKGEIQ DA
	Isoform E [UniParc]. Checksum: 2124C91421ADF507	FASTA	316	37,711
	10 20 30 40 50 60 MMGRHCHVPK QTHHIQFAQL AQSPAVCGLS ESAANLRYKL LLICHRVGIK IPRQKKIPTT 70 80 90 100 110 120 YNEQAILFYT PRWLWKSWEG GKIHALIMDL DIGICSEAEK KQKKKLLLDY LWENLRYHNW 130 140 150 160 170 180 WAYRYYVCEL LALINVIGQM FLMNRFFDGE FITFGLKVID YMETDQEDRM DPMIYIFPRM 190 200 210 220 230 240 TKCTFFKYGS SGEVEKHDAI CILPLNVVNE KIYIFLWFWF ILLTFLTLLT LIYRVVIIFS 250 260 270 280 290 300 PRMRVYLFRM RFRLVRRDAI EIIVRRSKMG DWFLLYLLGE NIDTVIFRDV VQDLANRLGH 310 NQHHRVPGLK GEIQDA
	Isoform F [UniParc]. Checksum: 2BEC859714E49365	FASTA	292	35,073
	10 20 30 40 50 60 MKKQGSEVPF PGVHNSQGRG PLTIKHTKYY QWVAFTLFFQ AILFYTPRWL WKSWEGGKIH 70 80 90 100 110 120 ALIMDLDIGI CSEAEKKQKK KLLLDYLWEN LRYHNWWAYR YYVCELLALI NVIGQMFLMN 130 140 150 160 170 180 RFFDGEFITF GLKVIDYMET DQEDRMDPMI YIFPRMTKCT FFKYGSSGEV EKHDAICILP 190 200 210 220 230 240 LNVVNEKIYI FLWFWFILLT FLTLLTLIYR VVIIFSPRMR VYLFRMRFRL VRRDAIEIIV 250 260 270 280 290 RRSKMGDWFL LYLLGENIDT VIFRDVVQDL ANRLGHNQHH RVPGLKGEIQ DA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a cDNA from the neurologically active locus shaking-B (Passover) of Drosophila melanogaster." Crompton D.E., Griffin A., Davies J.A., Miklos G.L.G. Gene 122:385-386(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Strain: Oregon-R. Tissue: Embryo.
[2]	"Passover: a gene required for synaptic connectivity in the giant fiber system of Drosophila." Krishnan S.N., Frei E., Swain G.P., Wyman R.J. Cell 73:967-977(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NEURAL), DISRUPTION PHENOTYPE. Strain: Oregon-R. Tissue: Pupae.
[3]	"Essential and neural transcripts from the Drosophila shaking-B locus are differentially expressed in the embryonic mesoderm and pupal nervous system." Crompton D., Todman M., Wilkin M., Ji S., Davies J. Dev. Biol. 170:142-158(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LETHAL). Tissue: Embryo.
[4]	"Molecular basis of intracistronic complementation in the Passover locus of Drosophila." Krishnan S.N., Frei E., Schalet A.P., Wyman R.J. Proc. Natl. Acad. Sci. U.S.A. 92:2021-2025(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LETHAL).
[5]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[6]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[7]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D). Strain: Berkeley.
[8]	"The shaking B gene in Drosophila regulates the number of gap junctions between photoreceptor terminals in the lamina." Shimohigashi M., Meinertzhagen I.A. J. Neurobiol. 35:105-117(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Drosophila Shaking-B protein forms gap junctions in paired Xenopus oocytes." Phelan P., Stebbings L.A., Baines R.A., Bacon J.P., Davies J.A., Ford C. Nature 391:181-184(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[10]	"Nested transcripts of gap junction gene have distinct expression patterns." Zhang Z., Curtin K.D., Sun Y.A., Wyman R.J. J. Neurobiol. 40:288-301(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M98872 mRNA. Translation: AAA89079.1. X65103 mRNA. Translation: CAA46228.1. L13306 mRNA. Translation: AAA28745.1. S78495 mRNA. Translation: AAB34769.1. U17330 mRNA. Translation: AAC46584.1. AE014298 Genomic DNA. Translation: AAF50880.2. AE014298 Genomic DNA. Translation: AAF50882.2. AE014298 Genomic DNA. Translation: AAF50883.1. AE014298 Genomic DNA. Translation: ABW09456.2. AE014298 Genomic DNA. Translation: ACL82952.1. BT025062 mRNA. Translation: ABE73233.1.
PIR	A40734. JN0441.
RefSeq	NP_001097038.2. NM_001103568.2. NP_001138222.1. NM_001144750.1. NP_523425.2. NM_078701.3. NP_608410.2. NM_134566.2. NP_728361.1. NM_170660.1.
UniGene	Dm.2607.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	1.A.25.1.4. gap junction-forming innexin family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0112571; FBpp0111483; FBgn0085387.
GeneID	33062.
KEGG	dme:Dmel_CG34358.
Organism-specific databases
CTD	33062.
FlyBase	FBgn0085387. shakB.
Phylogenomic databases
eggNOG	NOG74896.
GeneTree	ENSGT00530000064205.
InParanoid	Q9VRC2.
OMA	CAPRIRY.
OrthoDB	EOG4QBZMV.
Gene expression databases
Bgee	P33085.
GermOnline	CG32508. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000990. Innexin. [Graphical view]
PANTHER	PTHR11893. PTHR11893. 1 hit.
Pfam	PF00876. Innexin. 1 hit. [Graphical view]
PRINTS	PR01262. INNEXIN.
PROSITE	PS51013. PANNEXIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	33062.
NextBio	781749.

Entry information

Entry name

SHAKB_DROME

Accession

Primary (citable) accession number: P33085
Secondary accession number(s): A8JUT3 , B7Z104, Q1RKZ8, Q24011, Q24520, Q26432, Q9VRB9, Q9VRC0, Q9VRC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project