P33073 (SDHA_PEPAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-serine dehydratase, alpha chain
Short name=SDH
EC=4.3.1.17

Alternative name(s):
L-serine deaminase
Short name=L-SD

Gene names

Name:

sdhA

Organism

Peptostreptococcus asaccharolyticus (Peptococcus asaccharolyticus)

Taxonomic identifier

1258 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales incertae sedis › Clostridiales Family XI. Incertae Sedis › Peptoniphilus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-serine = pyruvate + NH3.
Cofactor	Binds 1 4Fe-4S cluster.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subunit structure	Heterooctamer of four alpha chains and four beta chains.
Sequence similarities	Belongs to the iron-sulfur dependent L-serine dehydratase family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW L-serine ammonia-lyase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	L-serine dehydratase, alpha chain		PRO_0000171915
Experimental info
	Sequence conflict	6	1	Missing AA sequence Ref.2
	Sequence conflict	12	1	C → Q AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P33073 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 4247702EEC932AE0	FASTA	292	30,770
	10 20 30 40 50 60 MLNTAREIID VCNERGIKIY DLVLEEEIKN SHTTEEEIRK KLDAVIDVMH ASATKNLTQS 70 80 90 100 110 120 DVTEYKMIDG FAKRTYEYAN SGKSIVGDFL AKAMAMAFST SEVNASMGKI VAAPTAGSSG 130 140 150 160 170 180 IMPAMLVAAT EKYNFDRTTI QNGFLTSIGI GQVITKYATF AGAEGGCQAE CGSASAMAAA 190 200 210 220 230 240 ALVEMLGGTV EQALHAASIT IINVLGLVCD PIAGLVQYPC TFRNASGVIN AFISADLALA 250 260 270 280 290 GVESLVPFDE VVIAMGEVGN SMIEALRETG LGGLAGSKTG QKIRRDFLKE GD

References

[1]

"Cloning and expression of the two genes coding for L-serine dehydratase from Peptostreptococcus asaccharolyticus: relationship of the iron-sulfur protein to both L-serine dehydratases from Escherichia coli." Hofmeister A.E., Textor S., Buckel W. J. Bacteriol. 179:4937-4941(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 14963 / DSM 20463 / NCIB 10074 / NCTC 11461.

[2]

"Purification and properties of an iron-sulfur-containing and pyridoxal-phosphate-independent L-serine dehydratase from Peptostreptococcus asaccharolyticus." Grabowski R., Buckel W. Eur. J. Biochem. 199:89-94(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-23. Strain: ATCC 14963 / DSM 20463 / NCIB 10074 / NCTC 11461.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U76260 Genomic DNA. Translation: AAC45546.1.
PIR	S16224.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00138.
Family and domain databases
InterPro	IPR005130. Ser_deHydtase-like_asu. IPR004642. Ser_deHydtase_asu. [Graphical view]
Pfam	PF03313. SDH_alpha. 1 hit. [Graphical view]
TIGRFAMs	TIGR00718. sda_alpha. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SDHA_PEPAS

Accession

Primary (citable) accession number: P33073
Secondary accession number(s): O33922

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	July 15, 1999
Last modified:	April 3, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program