ID   NTP1_VARV               Reviewed;         631 AA.
AC   P33066; Q9QNI4;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   16-JUN-2009, entry version 58.
DE   RecName: Full=Nucleoside triphosphatase I;
DE            EC=3.6.1.15;
DE   AltName: Full=Nucleoside triphosphate phosphohydrolase I;
DE            Short=NPH I;
GN   Name=NPH1; ORFNames=D11L, N1L;
OS   Variola virus.
OC   Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae;
OC   Orthopoxvirus.
OX   NCBI_TaxID=10255;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=India-1967 / Isolate Ind3;
RX   MEDLINE=93190624; PubMed=8383392; DOI=10.1016/0168-1702(93)90110-9;
RA   Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S.,
RA   Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V.,
RA   Gashikov P.V., Belanov E.F., Belavin P.A., Resenchuk S.M.,
RA   Andzhaparidze O.G., Sandakhchiev L.S.;
RT   "Nucleotide sequence analysis of variola virus HindIII M, L, I genome
RT   fragments.";
RL   Virus Res. 27:25-35(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=India-1967 / Isolate Ind3;
RX   MEDLINE=93202281; PubMed=8384129; DOI=10.1016/0014-5793(93)80041-R;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   MEDLINE=94088747; PubMed=8264798; DOI=10.1038/366748a0;
RA   Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R.,
RA   Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N.,
RA   Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J.,
RA   Venter J.C.;
RT   "Potential virulence determinants in terminal regions of variola
RT   smallpox virus genome.";
RL   Nature 366:748-751(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Garcia-1966;
RX   MEDLINE=20107289; PubMed=10639322; DOI=10.1006/viro.1999.0086;
RA   Shchelkunov S.N., Totmenin A.V., Loparev V.N., Safronov P.F.,
RA   Gutorov V.V., Chizhikov V.E., Knight J.C., Parsons J.M., Massung R.F.,
RA   Esposito J.J.;
RT   "Alastrim smallpox variola minor virus genome DNA sequences.";
RL   Virology 266:361-386(2000).
CC   -!- FUNCTION: Serves two roles in transcription; it acts in concert
CC       with viral termination factor/capping enzyme to catalyze release
CC       of UUUUUNU-containing nascent RNA from the elongation complex, and
CC       it acts by itself as a polymerase elongation factor to facilitate
CC       readthrough of intrinsic pause sites (By similarity).
CC   -!- CATALYTIC ACTIVITY: NTP + H(2)O = NDP + phosphate.
CC   -!- SIMILARITY: Belongs to the helicase family. NPH I subfamily.
CC   -!- SIMILARITY: Contains 1 helicase ATP-binding domain.
CC   -!- SIMILARITY: Contains 1 helicase C-terminal domain.
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DR   EMBL; X67119; CAA47600.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X69198; CAA49042.1; -; Genomic_DNA.
DR   EMBL; L22579; AAA60849.1; -; Genomic_DNA.
DR   EMBL; Y16780; CAB54701.1; -; Genomic_DNA.
DR   PIR; C72163; C72163.
DR   PIR; H36847; H36847.
DR   PIR; T28539; T28539.
DR   RefSeq; NP_042145.1; -.
DR   GeneID; 1486475; -.
DR   BRENDA; 3.6.1.15; 288297.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004386; F:helicase activity; IEA:InterPro.
DR   GO; GO:0006350; P:transcription; IEA:UniProtKB-KW.
DR   InterPro; IPR014001; DEAD-like_N.
DR   InterPro; IPR001650; DNA/RNA_helicase_C.
DR   InterPro; IPR014021; Helicase_SF1/SF2_ATP-bd.
DR   InterPro; IPR013676; NPHI_C.
DR   InterPro; IPR000330; SNF2_N.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF08469; NPHI_C; 1.
DR   Pfam; PF00176; SNF2_N; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Hydrolase; Late protein; Nucleotide-binding;
KW   Transcription.
FT   CHAIN         1    631       Nucleoside triphosphatase I.
FT                                /FTId=PRO_0000099093.
FT   DOMAIN       42    204       Helicase ATP-binding.
FT   DOMAIN      367    532       Helicase C-terminal.
FT   NP_BIND      55     62       ATP (By similarity).
FT   MOTIF       141    144       DEXH box.
FT   VARIANT     331    331       E -> K (in strain: Garcia-1966).
FT   VARIANT     577    577       E -> K (in strain: Garcia-1966).
SQ   SEQUENCE   631 AA;  72353 MW;  7C0BC2F82B534925 CRC64;
     MSKSHAAYID YALRRTTNMP VEMMGTDVVR LKDYQHFVAR VFLGLDSMHS LLLFHETGVG
     KTMTTVYILK HLKDIYTNWA IILLVKKALI EDPWMNTILR YAPEITKDCI FINYDDQNFR
     NKFFTNIKTI NSKSRICVII DECHNFISKS LIKEDGKIRP TRSVYNFLSK TIALKNHKMI
     CLSATPIVNS VQEFTMLVNL LRPGSLQHQS LFENKRLVNE KELVSKLGGL CSYIVNNEFS
     IFDDVEGSAS FAKKTVLMRY VNMSKKQEEI YQKAKLTEIK TGISSFRILR RMATTFTFDS
     FPERQNRDPG EYAQEIATLY NDFKRSLRDR EFSKSALDTF KKGELLGGDA SAADISLFTE
     LKEKSAKFID VCLGILASHG KCLVFEPFVN QSGIEILLLY FKVFGISNIE FSSRTKDTRI
     KAVAEFNQES NTNGECIKTC VFSSSGGEGI SFFSINDIFI LDMTWNEASL RQIVGRAIRL
     NSHVLTPPER RYVNVHFIMA RLSNGMPTVD EDLFEIIQSK SKEFVQLFRV FKHTSLEWIH
     ANEKDFSPID NESGWKTLVS RAIDLSSKKN ITNKLIEGTN IWYSNSNRLM SINRGFKGVD
     GRVYDVDGNY LHDMPDNPVI KIHDGKLIYI F
//