P33066 (NTP1_VAR67) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside triphosphatase I
EC=3.6.1.15

Alternative name(s):
NPH-I
Nucleoside triphosphate phosphohydrolase I
Short name=NPH I

Gene names

Name:	NPH1
ORF Names:	D11L, N1L

Organism

Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus) [Reference proteome]

Taxonomic identifier

587200 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Variola virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent ATPase required for providing the needed energy to achieve the termination of early transcripts. Acts in concert with the RAP94 subunit of the virion RNA polymerase and the capping enzyme/VTF to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex. NPH-I must bind ssDNA in order to exhibit ATPase activity By similarity.
Catalytic activity	NTP + H2O = NDP + phosphate.
Subunit structure	Monomer. Interacts (via C-terminus) with RAP94 (via N-terminus). Interacts with the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase J3 By similarity.
Subcellular location	Virion By similarity. Note: Virion core enzyme By similarity.
Sequence similarities	Belongs to the helicase family. NPH I subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence CAA47600.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Virion
Developmental stage	Late protein
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 631	631	Nucleoside triphosphatase I		PRO_0000099093
Regions
	Domain	42 – 204	163	Helicase ATP-binding
	Domain	367 – 532	166	Helicase C-terminal
	Nucleotide binding	55 – 62	8	ATP By similarity
	Region	457 – 524	68	Binding to the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity
	Motif	141 – 144	4	DEXH box
Natural variations
	Natural variant	331	1	E → K in strain: Garcia-1966.
	Natural variant	577	1	E → K in strain: Garcia-1966.

Sequences

	Sequence		Length	Mass (Da)
	P33066 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 7C0BC2F82B534925	FASTA	631	72,353
	10 20 30 40 50 60 MSKSHAAYID YALRRTTNMP VEMMGTDVVR LKDYQHFVAR VFLGLDSMHS LLLFHETGVG 70 80 90 100 110 120 KTMTTVYILK HLKDIYTNWA IILLVKKALI EDPWMNTILR YAPEITKDCI FINYDDQNFR 130 140 150 160 170 180 NKFFTNIKTI NSKSRICVII DECHNFISKS LIKEDGKIRP TRSVYNFLSK TIALKNHKMI 190 200 210 220 230 240 CLSATPIVNS VQEFTMLVNL LRPGSLQHQS LFENKRLVNE KELVSKLGGL CSYIVNNEFS 250 260 270 280 290 300 IFDDVEGSAS FAKKTVLMRY VNMSKKQEEI YQKAKLTEIK TGISSFRILR RMATTFTFDS 310 320 330 340 350 360 FPERQNRDPG EYAQEIATLY NDFKRSLRDR EFSKSALDTF KKGELLGGDA SAADISLFTE 370 380 390 400 410 420 LKEKSAKFID VCLGILASHG KCLVFEPFVN QSGIEILLLY FKVFGISNIE FSSRTKDTRI 430 440 450 460 470 480 KAVAEFNQES NTNGECIKTC VFSSSGGEGI SFFSINDIFI LDMTWNEASL RQIVGRAIRL 490 500 510 520 530 540 NSHVLTPPER RYVNVHFIMA RLSNGMPTVD EDLFEIIQSK SKEFVQLFRV FKHTSLEWIH 550 560 570 580 590 600 ANEKDFSPID NESGWKTLVS RAIDLSSKKN ITNKLIEGTN IWYSNSNRLM SINRGFKGVD 610 620 630 GRVYDVDGNY LHDMPDNPVI KIHDGKLIYI F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence analysis of variola virus HindIII M, L, I genome fragments." Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S., Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V., Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G., Sandakhchiev L.S. Virus Res. 27:25-35(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms." Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S. FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Potential virulence determinants in terminal regions of variola smallpox virus genome." Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C. Nature 366:748-751(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bangladesh-1975.
[4]	"Alastrim smallpox variola minor virus genome DNA sequences." Shchelkunov S.N., Totmenin A.V., Loparev V.N., Safronov P.F., Gutorov V.V., Chizhikov V.E., Knight J.C., Parsons J.M., Massung R.F., Esposito J.J. Virology 266:361-386(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Garcia-1966.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67119 Genomic DNA. Translation: CAA47600.1. Different initiation. X69198 Genomic DNA. Translation: CAA49042.1. L22579 Genomic DNA. Translation: AAA60849.1. Y16780 Genomic DNA. Translation: CAB54701.1.
PIR	C72163. H36847. T28539.
RefSeq	NP_042145.1. NC_001611.1.
3D structure databases
ProteinModelPortal	P33066.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1486475.
Phylogenomic databases
ProtClustDB	CLSP2509799.
Family and domain databases
InterPro	IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR013676. NPHI_C. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF08469. NPHI_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP1_VAR67

Accession

Primary (citable) accession number: P33066
Secondary accession number(s): Q9QNI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	May 30, 2000
Last modified:	April 3, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program