P33066 (NTP1_VAR67) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 76.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Nucleoside triphosphatase I EC=3.6.1.15 Alternative name(s): NPH-I Nucleoside triphosphate phosphohydrolase I Short name=NPH I | ||||
| Gene names |
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| Organism | Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus) [Reference proteome] | ||||
| Taxonomic identifier | 587200 [NCBI] | ||||
| Taxonomic lineage | Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › ![]() | ||||
| Virus host | Homo sapiens (Human) [TaxID: 9606] |
Protein attributes
| Sequence length | 631 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | DNA-dependent ATPase required for providing the needed energy to achieve the termination of early transcripts. Acts in concert with the RAP94 subunit of the virion RNA polymerase and the capping enzyme/VTF to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex. NPH-I must bind ssDNA in order to exhibit ATPase activity By similarity. |
| Catalytic activity | NTP + H2O = NDP + phosphate. |
| Subunit structure | Monomer. Interacts (via C-terminus) with RAP94 (via N-terminus). Interacts with the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase J3 By similarity. |
| Subcellular location | Virion By similarity. Note: Virion core enzyme By similarity. |
| Sequence similarities | Belongs to the helicase family. NPH I subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. |
| Sequence caution | The sequence CAA47600.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription |
| Cellular component | Virion |
| Developmental stage | Late protein |
| Ligand | ATP-binding DNA-binding Nucleotide-binding |
| Molecular function | Hydrolase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular_component | virion Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA bindingInferred from electronic annotation. Source: UniProtKB-KW helicase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 631 | 631 | Nucleoside triphosphatase I | PRO_0000099093 | |||||
Regions | |||||||||
| Domain | 42 – 204 | 163 | Helicase ATP-binding | ||||||
| Domain | 367 – 532 | 166 | Helicase C-terminal | ||||||
| Nucleotide binding | 55 – 62 | 8 | ATP By similarity | ||||||
| Region | 457 – 524 | 68 | Binding to the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity | ||||||
| Motif | 141 – 144 | 4 | DEXH box | ||||||
Natural variations | |||||||||
| Natural variant | 331 | 1 | E → K in strain: Garcia-1966. | ||||||
| Natural variant | 577 | 1 | E → K in strain: Garcia-1966. | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X67119 Genomic DNA. Translation: CAA47600.1. Different initiation. X69198 Genomic DNA. Translation: CAA49042.1. L22579 Genomic DNA. Translation: AAA60849.1. Y16780 Genomic DNA. Translation: CAB54701.1. |
| PIR | C72163. H36847. T28539. |
| RefSeq | NP_042145.1. NC_001611.1. |
3D structure databases | |
| ProteinModelPortal | P33066. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1486475. |
Phylogenomic databases | |
| ProtClustDB | CLSP2509799. |
Family and domain databases | |
| InterPro | IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR013676. NPHI_C. IPR000330. SNF2_N. [Graphical view] |
| Pfam | PF00271. Helicase_C. 1 hit. PF08469. NPHI_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view] |
| SMART | SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view] |
| PROSITE | PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NTP1_VAR67 | ||||||||
| Accession | Primary (citable) accession number: P33066 Secondary accession number(s): Q9QNI4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Viral Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with
