Dual specificity protein phosphatase H1 - Variola virus (isolate Human/India/Ind3/1967) (VARV)

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P33064 (DUSP_VAR67) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 73. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Dual specificity protein phosphatase H1
EC=3.1.3.-
EC=3.1.3.48

Alternative name(s):
Late protein H1

Gene names

ORF Names:

H1L, I1L

Organism

Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus) [Reference proteome]

Taxonomic identifier

587200 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus ›

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/Tyrosine phosphatase which down-regulates cellular antiviral response by dephosphorylating activated host STAT1 and blocking interferon (IFN)-stimulated innate immune responses. Dephosphorylates the A17 protein By similarity.
Catalytic activity	Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate. Protein serine phosphate + H₂O = protein serine + phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Virion By similarity. Host cytoplasm By similarity. Note: Approximately 200 molecules of H1 are packaged within the virion and are essential for the viability of the virus By similarity.
Induction	Expressed in the late phase of the viral replicative cycle.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host STAT1 by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Viral immunoevasion
Cellular component	Host cytoplasm Virion
Developmental stage	Late protein
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC suppression by virus of host STAT1 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 171

171

Dual specificity protein phosphatase H1

PRO_0000094869

Sites

Active site

110

Phosphocysteine intermediate By similarity

Secondary structure

171

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P33064 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 1D58C54FF17BADF7
Show »

FASTA

171

19,746

        10         20         30         40         50         60 
MDKKSLYKYL LLRSTGDMRR AKSPTIMTRV TNNVYLGNYK NAMNAPSSEV KFKYVLNLTM 

        70         80         90        100        110        120 
DKYTLPNSNI NIIHIPLVDD TTTDISKYFD DVTAFLSKCD QRNEPVLVHC VAGVNRSGAM 

       130        140        150        160        170 
ILAYLMSKNK ESSPMLYFLY VYHSMRDLRG AFVENPSFKR QIIEKYVIDK N

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence analysis of variola virus HindIII M, L, I genome fragments." Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S., Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V., Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G., Sandakhchiev L.S. Virus Res. 27:25-35(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms." Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S. FEBS Lett. 319:80-83(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Potential virulence determinants in terminal regions of variola smallpox virus genome." Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C. Nature 366:748-751(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bangladesh-1975.
[4]	Shchelkunov S.N., Balkin I.V., Totmenin A.V., Resenchuk S.M., Blinov V.M., Sandakhchiev L.S. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Garcia-1966.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X67119 Genomic DNA. Translation: CAA47583.1.
S55844 Genomic DNA. Translation: AAB24680.1.
X69198 Genomic DNA. Translation: CAA49025.1.
L22579 Genomic DNA. Translation: AAA60832.1.
X76264 Genomic DNA. Translation: CAA53838.1.

PIR

B72161.
I36845.
T28522.

RefSeq

NP_042128.1. NC_001611.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2P4D	X-ray	1.80	A	1-171	[»]

ProteinModelPortal

P33064.

SMR

P33064. Positions 5-167.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1486439.

Phylogenomic databases

ProtClustDB

CLSP2509785.

Family and domain databases

InterPro

IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]

PANTHER

PTHR10159. PTHR10159. 1 hit.

Pfam

PF00782. DSPc. 1 hit.
[Graphical view]

SMART

SM00195. DSPc. 1 hit.
[Graphical view]

PROSITE

PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P33064.

Entry information

Entry name

DUSP_VAR67

Accession

Primary (citable) accession number: P33064

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents