Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dual specificity protein phosphatase H1

Gene

H1L

Organism
Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Serine/Tyrosine phosphatase which down-regulates cellular antiviral response by dephosphorylating activated host STAT1 and blocking interferon (IFN)-stimulated innate immune responses. Dephosphorylates the A17 protein (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
Protein serine phosphate + H2O = protein serine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei110Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host STAT1 by virus, Viral immunoevasion

Enzyme and pathway databases

SABIO-RKP33064.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase H1 (EC:3.1.3.-, EC:3.1.3.48)
Alternative name(s):
Late protein H1
Gene namesi
ORF Names:H1L, I1L
OrganismiVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Taxonomic identifieri587200 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000002060 Componenti: Genome

Subcellular locationi

  • Virion By similarity
  • Host cytoplasm By similarity

  • Note: Approximately 200 molecules of H1 are packaged within the virion and are essential for the viability of the virus.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948691 – 171Dual specificity protein phosphatase H1Add BLAST171

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

1171
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 14Combined sources9
Beta strandi31 – 37Combined sources7
Helixi39 – 44Combined sources6
Helixi45 – 47Combined sources3
Beta strandi53 – 57Combined sources5
Beta strandi59 – 61Combined sources3
Beta strandi71 – 74Combined sources4
Beta strandi80 – 83Combined sources4
Helixi86 – 88Combined sources3
Helixi89 – 102Combined sources14
Beta strandi106 – 109Combined sources4
Beta strandi111 – 115Combined sources5
Helixi116 – 128Combined sources13
Helixi134 – 149Combined sources16
Helixi156 – 166Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P4DX-ray1.80A1-171[»]
ProteinModelPortaliP33064.
SMRiP33064.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33064.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK14165.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKSLYKYL LLRSTGDMRR AKSPTIMTRV TNNVYLGNYK NAMNAPSSEV
60 70 80 90 100
KFKYVLNLTM DKYTLPNSNI NIIHIPLVDD TTTDISKYFD DVTAFLSKCD
110 120 130 140 150
QRNEPVLVHC VAGVNRSGAM ILAYLMSKNK ESSPMLYFLY VYHSMRDLRG
160 170
AFVENPSFKR QIIEKYVIDK N
Length:171
Mass (Da):19,746
Last modified:October 1, 1993 - v1
Checksum:i1D58C54FF17BADF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67119 Genomic DNA. Translation: CAA47583.1.
S55844 Genomic DNA. Translation: AAB24680.1.
X69198 Genomic DNA. Translation: CAA49025.1.
L22579 Genomic DNA. Translation: AAA60832.1.
X76264 Genomic DNA. Translation: CAA53838.1.
PIRiB72161.
I36845.
T28522.
RefSeqiNP_042128.1. NC_001611.1.

Genome annotation databases

GeneIDi1486439.
KEGGivg:1486439.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67119 Genomic DNA. Translation: CAA47583.1.
S55844 Genomic DNA. Translation: AAB24680.1.
X69198 Genomic DNA. Translation: CAA49025.1.
L22579 Genomic DNA. Translation: AAA60832.1.
X76264 Genomic DNA. Translation: CAA53838.1.
PIRiB72161.
I36845.
T28522.
RefSeqiNP_042128.1. NC_001611.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P4DX-ray1.80A1-171[»]
ProteinModelPortaliP33064.
SMRiP33064.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1486439.
KEGGivg:1486439.

Phylogenomic databases

KOiK14165.

Enzyme and pathway databases

SABIO-RKP33064.

Miscellaneous databases

EvolutionaryTraceiP33064.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUSP_VAR67
AccessioniPrimary (citable) accession number: P33064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.