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Protein

Dual specificity protein phosphatase H1

Gene

H1L

Organism
Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Serine/Tyrosine phosphatase which down-regulates cellular antiviral response by dephosphorylating activated host STAT1 and blocking interferon (IFN)-stimulated innate immune responses. Dephosphorylates the A17 protein (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
Protein serine phosphate + H2O = protein serine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei110 – 1101Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. protein tyrosine/serine/threonine phosphatase activity Source: InterPro
  2. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. suppression by virus of host STAT1 activity Source: UniProtKB-KW
  2. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host STAT1 by virus, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase H1 (EC:3.1.3.-, EC:3.1.3.48)
Alternative name(s):
Late protein H1
Gene namesi
ORF Names:H1L, I1L
OrganismiVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Taxonomic identifieri587200 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002060 Componenti: Genome

Subcellular locationi

Virion By similarity. Host cytoplasm By similarity
Note: Approximately 200 molecules of H1 are packaged within the virion and are essential for the viability of the virus.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 171171Dual specificity protein phosphatase H1PRO_0000094869Add
BLAST

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

1
171
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 149Combined sources
Beta strandi31 – 377Combined sources
Helixi39 – 446Combined sources
Helixi45 – 473Combined sources
Beta strandi53 – 575Combined sources
Beta strandi59 – 613Combined sources
Beta strandi71 – 744Combined sources
Beta strandi80 – 834Combined sources
Helixi86 – 883Combined sources
Helixi89 – 10214Combined sources
Beta strandi106 – 1094Combined sources
Beta strandi111 – 1155Combined sources
Helixi116 – 12813Combined sources
Helixi134 – 14916Combined sources
Helixi156 – 16611Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P4DX-ray1.80A1-171[»]
ProteinModelPortaliP33064.
SMRiP33064. Positions 5-167.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33064.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK14165.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P33064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKSLYKYL LLRSTGDMRR AKSPTIMTRV TNNVYLGNYK NAMNAPSSEV
60 70 80 90 100
KFKYVLNLTM DKYTLPNSNI NIIHIPLVDD TTTDISKYFD DVTAFLSKCD
110 120 130 140 150
QRNEPVLVHC VAGVNRSGAM ILAYLMSKNK ESSPMLYFLY VYHSMRDLRG
160 170
AFVENPSFKR QIIEKYVIDK N
Length:171
Mass (Da):19,746
Last modified:September 30, 1993 - v1
Checksum:i1D58C54FF17BADF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67119 Genomic DNA. Translation: CAA47583.1.
S55844 Genomic DNA. Translation: AAB24680.1.
X69198 Genomic DNA. Translation: CAA49025.1.
L22579 Genomic DNA. Translation: AAA60832.1.
X76264 Genomic DNA. Translation: CAA53838.1.
PIRiB72161.
I36845.
T28522.
RefSeqiNP_042128.1. NC_001611.1.

Genome annotation databases

GeneIDi1486439.
KEGGivg:1486439.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67119 Genomic DNA. Translation: CAA47583.1.
S55844 Genomic DNA. Translation: AAB24680.1.
X69198 Genomic DNA. Translation: CAA49025.1.
L22579 Genomic DNA. Translation: AAA60832.1.
X76264 Genomic DNA. Translation: CAA53838.1.
PIRiB72161.
I36845.
T28522.
RefSeqiNP_042128.1. NC_001611.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P4DX-ray1.80A1-171[»]
ProteinModelPortaliP33064.
SMRiP33064. Positions 5-167.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1486439.
KEGGivg:1486439.

Phylogenomic databases

KOiK14165.

Miscellaneous databases

EvolutionaryTraceiP33064.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms."
    Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.
    FEBS Lett. 319:80-83(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bangladesh-1975.
  4. Shchelkunov S.N., Balkin I.V., Totmenin A.V., Resenchuk S.M., Blinov V.M., Sandakhchiev L.S.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Garcia-1966.

Entry informationi

Entry nameiDUSP_VAR67
AccessioniPrimary (citable) accession number: P33064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1993
Last sequence update: September 30, 1993
Last modified: March 31, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.