Reviewed, UniProtKB/Swiss-Prot P33057 (MCEL_VARV)

Names and origin

Protein names

Recommended name:
    mRNA-capping enzyme large subunit
Including the following 2 domains:
    1- Recommended name:
            Polynucleotide 5'-triphosphatase
              EC=3.1.3.33
        Alternative name(s):
            mRNA 5'-triphosphatase
              Short name=TPase
    2- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.50
        Alternative name(s):
            GTP--RNA guanylyltransferase
              Short name=GTase

Gene names

ORF Names:

D1R, F1R

Organism

Variola virus

Taxonomic identifier

10255 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	844 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the first two reactions in the mRNA cap formation pathway.
Catalytic activity	A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate. GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
Subunit structure	Heterodimer of a large and a small subunit.
Sequence similarities	Belongs to the viral GTase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC polynucleotide 5'-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 844	844	mRNA-capping enzyme large subunit		PRO_0000210125
Sites
	Active site	260	1	N6-GMP-lysine intermediate Potential
Natural variations
	Natural variant	118	1	A → E in strain: Bangladesh-1975 and Garcia-1966.
	Natural variant	552	1	E → D in strain: Garcia-1966.
	Natural variant	600	1	E → G in strain: Bangladesh-1975 and Garcia-1966.
	Natural variant	741	1	V → A in strain: Garcia-1966.
	Natural variant	831	1	D → N in strain: Garcia-1966.

Sequences

	Sequence		Length	Mass (Da)
	P33057-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: CAA2474D798115D1	FASTA	844	96,729
	10 20 30 40 50 60 MDANVVSSST IATYIDALAK NASELEQGST AYEINNELEL VFIKPPLITL TNVVNISTIQ 70 80 90 100 110 120 ESFIRFTVTN KEGVKIRTKI PLSKVHGLDV KNVQLVDAID NIVWEKKSLV TENRLHKACL 130 140 150 160 170 180 LRLSTEERHI FLDYKKYGSS IRLELVNLIQ AKTKNFTIDF KLKYFLGSGA QSKSSLLHAI 190 200 210 220 230 240 NHPKSRPNTS LEIEFTPRDN ETVPYDELIK ELTTFSRHIF MASPENVILS PPINAPIKTF 250 260 270 280 290 300 MLPKQDIVGM DLENLYAVTK TDGIPITIRV TSKGLYCYFT HLGYIIRYPV KRIIDSEVVV 310 320 330 340 350 360 FGEAVKDKNW TVYLIKLIEP VNAISDRLEE SKYVESKLVD ICDRIVFKSK KYEGPFTTTS 370 380 390 400 410 420 EVVDMLSTYL PKQPEGVILF YSKGPKSNID FKIKKENTID QTVNVVFRYM SSEPIIFGES 430 440 450 460 470 480 SIFIEYKKFT NDKGFPKEYG SGKIVLYNGV NYLNNIYCLE YINTHNEVGI KSVVVPIKFI 490 500 510 520 530 540 AEFLVNGEIL KPRIDKTMKY INSEDYYGNQ HNVIVEHLRD QSIKIGDVFN EDKLSDVGHQ 550 560 570 580 590 600 YANNDKFRLN PEVSYFTNKR TRGPLGILSN YVKTLLISMY CSKTFLDDSN KRKVLAIDFE 610 620 630 640 650 660 NGADLEKYFY GEIALLVATD PDADAIARGN ERYNKLNSGI KTKYYKFDYI QETIRSNTFV 670 680 690 700 710 720 SSVREVFYFG KFNIIDWQFA IHYSFHPRHY ATIMNNLSEL TASGGKVLIT TMDGDKLSKL 730 740 750 760 770 780 TDKKTFIIHK NLPSSENYMS VEKIADDRIV VYNPSTMSTP MTEYIIKKND IVRVFNEYGF 790 800 810 820 830 840 VLVDNVDFAT IIERSKKFIN GASTMEDRPS TRNFFELNRG AIKCEGLDVE DLLSYYVVYV FSKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence analysis of variola virus HindIII M, L, I genome fragments." Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S., Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V., Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G., Sandakhchiev L.S. Virus Res. 27:25-35(1993) [PubMed: 8383392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: India-1967 / Isolate Ind3.
[2]	"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms." Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S. FEBS Lett. 319:80-83(1993) [PubMed: 8384129] [Abstract] Cited for: COMPLETE GENOME. Strain: India-1967 / Isolate Ind3.
[3]	"Potential virulence determinants in terminal regions of variola smallpox virus genome." Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C. Nature 366:748-751(1993) [PubMed: 8264798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bangladesh-1975.
[4]	"Alastrim smallpox variola minor virus genome DNA sequences." Shchelkunov S.N., Totmenin A.V., Loparev V.N., Safronov P.F., Gutorov V.V., Chizhikov V.E., Knight J.C., Parsons J.M., Massung R.F., Esposito J.J. Virology 266:361-386(2000) [PubMed: 10639322] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Garcia-1966.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67119 Genomic DNA. Translation: CAA47590.1. X69198 Genomic DNA. Translation: CAA49032.1. L22579 Genomic DNA. Translation: AAA60839.1. Y16780 Genomic DNA. Translation: CAB54691.1.
PIR	A72162. G36846. T28529.
RefSeq	NP_042135.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1486417.
Enzyme and pathway databases
BRENDA	2.7.7.50. 288297. 3.1.3.33. 288297.
Family and domain databases
InterPro	IPR019602. mRNA_cap_ATPase/GuylTrfase_vir. IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF10640. Pox_ATPase-GT. 1 hit. PF03291. Pox_MCEL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCEL_VARV

Accession

Primary (citable) accession number: P33057
Secondary accession number(s): Q9QNI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)