Reviewed, UniProtKB/Swiss-Prot P33051 (NTP2_VARV)

Names and origin

Protein names

Recommended name:
    Nucleoside triphosphatase II
      Short name=NTPase II
    EC=3.6.1.15
Alternative name(s):
    Nucleoside triphosphate phosphohydrolase II
      Short name=NPH II
    RNA helicase I8

Gene names

Name:	NPH2
ORF Names:	I8R, K8R, L8R

Organism

Variola virus

Taxonomic identifier

10255 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	676 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential for viral replication. Plays an important role during transcription of early mRNAs, presumably by preventing R-loop formation behind the elongating RNA polymerase. Acts as NTP-dependent helicase that catalyzes unidirectional unwinding of 3'tailed duplex RNAs. Might also play a role in the export of newly synthesized mRNA chains out of the core into the cytoplasm. Required for propagation of viral particles.
Catalytic activity	NTP + H2O = NDP + phosphate.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 676	676	Nucleoside triphosphatase II		PRO_0000055186
Regions
	Domain	172 – 347	176	Helicase ATP-binding
	Domain	366 – 542	177	Helicase C-terminal
	Nucleotide binding	185 – 192	8	ATP By similarity
	Motif	296 – 299	4	DEXH box
Natural variations
	Natural variant	33	1	M → T in strain: Garcia-1966.
	Natural variant	147	1	G → A in strain: Garcia-1966.
	Natural variant	450	1	A → T in strain: Bangladesh-1975 and Garcia-1966.
	Natural variant	595	1	E → K in strain: Garcia-1966.
	Natural variant	632	1	D → DNDNDND in strain: Garcia-1966.

Sequences

	Sequence		Length	Mass (Da)
	P33051-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 4D7589E5B012562F	FASTA	676	77,545
	10 20 30 40 50 60 MEKNLPDIFF FPNCVNVFSY KYSQDEFSNM SNMERDSFSL AVFPVIKHRW HNAHVVKHKG 70 80 90 100 110 120 IYKVSTEAHG KKVSPPSLGK PSHINLTAKQ YIYSEHTISF ECYSFLKCIT NAEINSFDEY 130 140 150 160 170 180 ILRGLLEAGN SLQIFSNSVG KRTDTIGVLG NKYPFSKIPL ASLTPKAQRE IFSAWISHRP 190 200 210 220 230 240 VVLTGGTGVG KTSQVPKLLL WFNYLFGGFS TLDKITDFHE RPVILSLPRI ALVRLHSNTI 250 260 270 280 290 300 LKSLGFKVLD GSPISLRYGS IPEELINKQP KKYGIVFSTH KLSLTKLFSY GTLIIDEVHE 310 320 330 340 350 360 HDQIGDIIIA VARKHHTKID SMFLMTATLE DDRERLKVFL PNPAFIHIPG DTLFKISEVF 370 380 390 400 410 420 IHNKINPSSR MAYIEEEKRN LVTAIQMYTP PDGSSGIVFV ASVAQCHEYK SYLEKRLPYD 430 440 450 460 470 480 MYIIHGKVLE IDKILEKVYS SPNVSIIISA PYLESSVTIH NVTHIYDMGR VFVPAPFGGS 490 500 510 520 530 540 QQFISKSMRD QRKGRVGRVN PGTYVYFYDL SYMKSIQRIN SEFLHNYILY ANKFNLTLPE 550 560 570 580 590 600 DLFIIPTNLD ILWRTKEYID SFDISTETWN KLLSNYYMKM IEYAKLYVLS PILAEELDNF 610 620 630 640 650 660 ERTGELTSIV QEAILSLNLR IKILNFKHKD NDTYIHFCKI LFGVYNGTNA TIYYHRPLTG 670 YMNMISDTIF VPVDNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of a 43 kbp segment of the genome of variola virus India-1967 strain." Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Sandakhchiev L.S. Virus Res. 30:239-258(1993) [PubMed: 8109158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: India-1967 / Isolate Ind3.
[2]	"Nucleotide sequence analysis of variola virus HindIII M, L, I genome fragments." Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S., Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V., Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G., Sandakhchiev L.S. Virus Res. 27:25-35(1993) [PubMed: 8383392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: India-1967 / Isolate Ind3.
[3]	Blinov V.M. Submitted (NOV-1992) to the EMBL/GenBank/DDBJ databases Cited for: COMPLETE GENOME. Strain: India-1967 / Isolate Ind3.
[4]	"Potential virulence determinants in terminal regions of variola smallpox virus genome." Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C. Nature 366:748-751(1993) [PubMed: 8264798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bangladesh-1975.
[5]	"Alastrim smallpox variola minor virus genome DNA sequences." Shchelkunov S.N., Totmenin A.V., Loparev V.N., Safronov P.F., Gutorov V.V., Chizhikov V.E., Knight J.C., Parsons J.M., Massung R.F., Esposito J.J. Virology 266:361-386(2000) [PubMed: 10639322] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Garcia-1966.

Web resources

The DExH/D protein family database

Poxviridae DExH proteins

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67119 Genomic DNA. Translation: CAA47562.1. X69198 Genomic DNA. Translation: CAA49003.1. L22579 Genomic DNA. Translation: AAA60810.1. Y16780 Genomic DNA. Translation: CAB54662.1. X76267 Genomic DNA. Translation: CAA53867.1.
PIR	D72158. F36843. T28500.
RefSeq	NP_042106.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1486463.
Enzyme and pathway databases
BRENDA	3.6.1.15. 288297.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR014021. Helicase_SF1/SF2_ATP-bd. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP2_VARV

Accession

Primary (citable) accession number: P33051
Secondary accession number(s): Q85378, Q89208, Q9QNJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)